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猪流行性腹泻（Porcine epidemic diarrhea,PED）是由猪流行性腹泻病毒（Porcine epidemic diarrhea virus,PEDV）引起的一种高度接触性肠道传染病,以腹泻、呕吐、脱水和对哺乳仔猪高致死率为主要特征,给养猪业带来严重的经济损失。及时准确地诊断PEDV感染对于预防和控制PED至关重要,目前已经开发了许多用于PEDV、病毒蛋白及核酸的检测方法,包括病毒分离、酶联免疫吸附试验、直接免疫荧光检测、免疫组织化学技术、免疫层析技术等免疫学方法;RT-PCR、实时定量RT-PCR、核酸等温扩增技术、CRISPR-Cas系统检测技术等分子生物学方法。本文针对PEDV的抗原检测技术和方法进行综述,以期为PEDV的临床监测和PED的防控提供参考。 相似文献
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猪流行性腹泻病毒(Porcine epidemic diarrhea virus, PEDV)是猪流行性腹泻(Porcine epidemic diarrhea, PED)的病原,PEDV可导致猪的急性腹泻、脱水甚至死亡。如今PEDV已成为引起猪腹泻最常见病原体之一,给我国养猪行业造成极大的经济损失。PEDV的四种结构蛋白在病毒结构、感染宿主、复制和组装过程中发挥着重要功能,同时在逃避宿主天然免疫方面也起着重要作用。阐述了PEDV四种结构蛋白Spike(S)蛋白、Membrane(M)蛋白、Nucleocapsid(N)蛋白、Envelope(E)蛋白在病毒感染中与宿主细胞的互作机制,为PEDV防控以及疫苗研发等提供理论研究基础。 相似文献
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猪流行性腹泻（Porcine epidemic diarrhea,PED）是由猪流行性腹泻病毒（Porcine epidemic diarrhea virus,PEDV）引起的以腹泻、呕吐、脱水和对哺乳仔猪高致死率为主要特征的一种高度接触性肠道传染病。从2010年10月份开始, 相似文献
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近年来，猪流行性腹泻(porcine epidemic diarrhea, PED)给我国畜牧业造成了严重的经济损失。随着人们对猪流行性腹泻病毒(Porcine epidemic diarrhea virus, PEDV)研究的深入，其致病机制也逐渐被揭晓，即PEDV进入宿主体内通过抑制干扰素的表达和诱导小肠细胞凋亡等机制对宿主产生严重影响。文章综述了PEDV蛋白组成及其对肠黏膜屏障和细胞影响的分子机制，以期为PED科学防控提供参考。 相似文献
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猪流行性腹泻(Poiineepidemic diarrhea,PED)是由猪流行性腹泻病毒(Porcine epidemic diarrheaviru—PEDV)引細-种|性高度接触性肠道传染病。以呕吐、水样腹泻和脱水为特征,发病仔猪死亡率高。当前由PEDV主导的猪群腹泻是猪场重点防控的重要疫病之一,严重影响猪场经济效益及养猪业健康发展,其防控形势依然严峻。1971年英国首次报道PED,1977年比利时分离到病毒(经典代表毒株CV777)。 相似文献
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猪流行性腹泻(Porcine epidemic diarrhea.PED)是由猪流行性腹泻病毒(Porcine epidemic diarrhea virus.PEDV)引起的以呕吐、腹泻、脱水和对哺乳仔猪高致死率为主要特征的一种高度接触性肠道传染病。1971年首发于英格兰地区,随后该病在比利时等地暴发,自20世纪80年代,我国山东、上海、辽宁、吉林、黑龙江、四川等地陆续有本病发生的报道。该病给养猪业造成了巨大的经济损失。本文主要针对猪流行性腹泻病毒在流行病学、临床症状、病理变化以及诊断、预防与治疗措施进行概括,旨在为该病的防控提供理论基础和技术参考。1猪流行性腹泻的流行病学特点 相似文献
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猪流行性腹泻病毒(Porcine epidemic diarrhea virus, PEDV)是引起仔猪严重肠道疾病的病原之一,新生仔猪感染后常引起致死性水样腹泻、呕吐、出血性肠炎,死亡率高达100%,给全球养猪业带来了巨大的经济损失。2010年,高致病性PEDV变异毒株的出现与流行造成猪流行性腹泻(PED)在全球的再次暴发,以经典毒株为抗原的流行性腹泻疫苗无法有效控制高致病性变异毒株的流行。文章综述了PEDV基因组结构与蛋白功能、基因分型与流行现状,以期为PEDV新型疫苗的研究提供参考。 相似文献
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猪流行性腹泻病毒（PEDV）是高度接触性肠道传染病猪流行性腹泻（PED）的病原,是有囊膜的单股正链RNA病毒,基因组全长约28 kb。2010年以来,PEDV G2型高致病性毒株不断发生变异,给全国乃至全球的养猪业造成巨大的经济损失。反向遗传学系统,即构建RNA病毒的全长感染性克隆。近年来,PEDV主要基于靶向RNA重组、BAC系统和体外连接3种方法来建立全长感染性cDNA克隆。文章简述了反向遗传学的原理和方法。靶向RNA重组利用冠状病毒RNA的高同源重组的特点来实现病毒的拯救;BAC系统利用pBeloBAC11载体克服PEDV基因组中含有的毒性序列所导致的cDNA在高拷贝质粒中不稳定的困难;体外连接技术主要利用PEDV基因组本身存在的限制性内切酶的酶切位点或通过改造的酶切位点在体外将病毒分片段地连接成全长的cDNA克隆。另外,文章还总结了近年来基于反向遗传学技术的PEDV相关的研究进展。PEDV反向遗传学是研究PEDV病毒基因组结构功能及设计减毒活疫苗的有效工具,利用反向遗传学技术探究S基因等毒力相关基因,探究其突变或缺失对病毒致病机制的影响,揭示PEDV毒力衰减的分子机制,有望设计出具有良好免疫原性且避免毒株返毒和重组减毒活疫苗。总之,PEDV反向遗传学是研究PEDV基因组结构及功能、病毒宿主相互作用及致病机制的一种重要方法,同时也是设计PEDV减毒活疫苗一种合理有效的途径。 相似文献
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Gao Xing Zhang Lihong Jiang Xiong Mehmood Khalid Wang Lei Tong Xiaole Wang Meng Zhang Hui Li Jiakui 《Tropical animal health and production》2019,51(2):491-494
Tropical Animal Health and Production - Porcine epidemic diarrhea virus (PEDV) is a devastating cause of diarrhea in pigs worldwide, and has caused enormous economic losses in the pork industry. To... 相似文献
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猪流行性腹泻（porcine epidemic diarrhea,PED）是危害养猪业健康发展的重要疾病之一,具有急性、高度传染性的特征,给养猪业造成了严重的经济损失。猪流行性腹泻病毒（porcine epidemic diarrhea virus,PEDV）感染会破坏动物机体的消化系统,造成患病猪食欲下降、呕吐以及严重腹泻等,且药物治愈后的仔猪也会因为生长发育不良等因素严重影响生产,给养殖户带来巨大的困扰。2010年PEDV高致病变异毒株在中国暴发流行,导致PED的发病率和死亡率大幅升高,严重影响中国养猪生产。此次PED的暴发流行引起了养猪行业的密切关注,相关研究领域的学者对PEDV致病机制和PED新型疫苗进行了更加深入的研究,亚单位疫苗、病毒活载体疫苗、细菌活载体疫苗、转基因植物疫苗和核酸疫苗5种PED新型疫苗相继被开发,并取得全新的突破和进展。与传统的PED灭活苗和PED弱毒疫苗相比,PED新型基因工程疫苗具有安全性好、制备简单、免疫效果好等优点。文章着重对PEDV发病机理和5种PED新型疫苗的研究进展展开综述,从而加深对PEDV和PED新型疫苗的了解,以期为PEDV感染的预防和控制措施提供参考。 相似文献
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Porcine epidemic diarrhea (PED) is one of the important diseases that endanger the healthy development of the pig industry.It has acute and highly infectious characteristics,causing severe economic losses to the pig industry.Porcine epidemic diarrhea virus (PEDV) infection could destroy the animal's digestive system,cause the appetite of sick pigs to decline,vomit,and severe diarrhea.And the piglets cured by drugs will also have a serious impact on production due to factors such as poor growth and development,which will bring huge problems to the farmers.The outbreak of PEDV highly pathogenic mutant strains in China in 2010 led to a significant increase in the incidence and mortality of PED,which severely affected pig production in China.The outbreak of PED has drawn close attention from the pig industry.Scholars in related research fields have conducted more in-depth research on the pathogenic mechanism of PEDV and the new PED vaccine.Five new types of PED vaccines,including subunit vaccine,virus live vector vaccine,bacterial live vector vaccine,transgenic plant vaccine and nucleic acid vaccine,have been developed successively,and new breakthroughs and progress have been made.Compared with the traditional PED inactivated vaccine and PED attenuated vaccine,the new PED genetically engineered vaccine has the advantages of good safety,simple preparation,and good immune effect.This article focuses on the pathogenesis of PEDV and the research progress of five new PED vaccines,so as to deepen our understanding of new PEDV and PED vaccines,in order to provide references for prevention and control measures of PEDV infection. 相似文献
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猪流行性腹泻病毒检测方法研究进展 总被引：1，自引：0，他引：1
介绍了猪流行性腹泻病毒实验室检测方法的研究进展,在细胞生物学方面,主依靠病毒分离培养、病毒电镜形态检查、病毒特异性抗原及其抗体的检查等检测方法;在分子生物学方面,主依靠核酸杂交技术、RT—PCR法和实时荧光定量RT—PCR法等检测方法。介绍和比较了各种方法的优缺点和实用性,为临床兽医科技工作者提供参考。 相似文献
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Kimberly Crawford Kelly Lager Laura Miller Tanja Opriessnig Priscilla Gerber Richard Hesse 《Veterinary research》2015,46(1)
Clinical disease associated with porcine epidemic diarrhea virus (PEDV) infection in naïve pigs is well chronicled; however, information on endemic PEDV infection is limited. To characterize chronic PEDV infection, the duration of infectious virus shedding and development of protective immunity was determined. On Day 0 (D0), a growing pig was challenged with PEDV and 13 contacts were commingled. On D7, 9 contact pigs (principal virus group (PG)), were selected, moved to a separate room and commingled with one sentinel pig (S1). This process was repeated weekly with S2, S3 and S4. The PG was PEDV-positive by PCR from D3-11, with some pigs intermittently positive to D42. Pigs S1 and S2 were PEDV-positive within 24 hours of commingling. Antibodies were detected in all PG by D21 and by 7 days post-contact in S1 and S2. Pigs S3 and S4 were PCR and antibody negative following commingling. To evaluate protective immunity, 5 naïve pigs (N) and the PG were challenged (N/C, PG/C) with homologous virus on D49. All N/C pigs were PEDV PCR-positive by D52 with detection out to D62 in 3/5 N/C pigs. All PG/C pigs were PEDV PCR-negative post-challenge. By D63, all N/C seroconverted. Although PEDV RNA was demonstrated in pigs after primary infection until D42, infectious PEDV capable of horizontal transmission to naïve pigs was only shed 14–16 days after infection to age-matched pigs. Homologous re-challenge 49 days post initial PEDV exposure did not result in re-infection of the pigs. This demonstrates potential for an effective PEDV vaccine. 相似文献
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猪流行性腹泻病毒(PEDV)属于冠状病毒家族成员,是近年来引起新生仔猪水样腹泻致死的主要病原之一,给养殖业带来了巨大威胁。病毒感染后,宿主细胞通过模式识别受体(PRRs)识别病原相关分子模式(PAMPs),促进I型干扰素等细胞因子的产生,进而抑制病毒的增殖。近年来的研究表明,PEDV能够通过其编码蛋白对宿主的抗病毒天然免疫反应进行调节,成功逃避免疫识别或拮抗宿主的天然免疫反应,为自身的快速复制和增殖创造条件。本文结合目前关于PEDV与宿主细胞相互作用的研究进展,综合分析了PEDV编码的各种蛋白在感染过程中拮抗宿主天然免疫系统的分子机制,旨在为进一步认识PEDV乃至其他冠状病毒的致病机制,也为探索新的抗病毒药物靶标提供思路。 相似文献
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Kweon CH Kwon BJ Woo SR Kim JM Woo GH Son DH Hur W Lee YS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(9):961-964
Porcine epidemic diarrhea virus (PEDV) is the causative agent of neonatal diarrhea in piglets, which causes high mortality rates. In this study, the immunoprophylactic effects of chicken egg yolk immunoglobulin (Ig Y) against PEDV were investigated in neonatal pigs. Ig Y was found to reduce the mortality in piglets after challenge exposures. The field application of Ig Y also revealed significant differences in survival rates of piglets given Ig Y, as compared with placebo or control. The results in this study indicated that Ig Y against PEDV could be an alternative way of supplementing prophylactic measures like colostral antibodies from sows. 相似文献
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Enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea coronavirus antibodies in swine sera 总被引：1，自引：0，他引：1
An enzyme-linked immunosorbent assay (ELISA) for detecting serum antibodies to the porcine epidemic diarrhea coronavirus (PEDV) was established by using cell culture-grown PEDV as antigen for coating. Ultracentrifugation through 20 and 45% (w/w) sucrose cushions proved to be the best antigen purification method. Examination of 1024 swine sera showed a high specificity and a greater sensitivity of the ELISA, when compared with indirect immunofluorescence. Reference sera with high antibody titers to PEDV originated from two pigs experimentally infected with PEDV. Three different antigen purification methods and the advantages of the ELISA compared with an immunofluorescence test are discussed. 相似文献
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PEDV,TGEV and PoRV are the main pathogenies of porcine viral diarrhea that endanger the healthy development of swine industry,which are characterized by vomiting,severe diarrhea and dehydration, the morbidity and mortality of suckling piglets is higher,and the incidence of diarrhea is lower in replacement gilts,sows or fattening pigs. Especially since 2010, the prevalence of PEDV mutant strains has had a serious impact on the global swine industry,but the control measures can not been able to cope with the new epidemic situation. In recent years, many new techniques or methods have been applied to the control of swine viral diarrhea caused by PEDV, TGEV and PoRV. Based on the analysis of genetic variation and epidemic status of three kinds of viruses,this paper reviewed the prevention and control measures from the aspects of feeding management,immunization prevention, traditional Chinese medicine therapy,interference therapy,specific therapy and return therapy. 相似文献