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高效液相色谱-串联质谱法检测猪肝中苯并咪唑类药物及其代谢物的残留量 总被引：2，自引：0，他引：2
建立了猪肝中阿苯哒唑及其代谢物阿苯哒唑砜、阿苯哒唑亚砜、2-氨基阿苯哒唑砜,噻苯哒唑及其代谢物5-羟基噻苯哒唑,甲苯哒唑及其代谢物氨基甲苯哒唑、5-羟基甲苯哒唑,芬苯哒唑及其代谢物芬苯哒唑砜、等效物奥芬哒唑,氟苯哒唑及其代谢物2-氨基-氟苯哒唑共14种苯并咪唑类药物及其代谢物残留检测的高效液相色谱-串联质谱方法。样品用乙酸乙酯提取,MCX固相萃取柱净化。Waters Xterra C18色谱柱(2.1 mm×150 mm,5μm)分离,流动相A相为0.1%甲酸乙腈溶液;B相为0.1%甲酸水溶液,梯度洗脱,外标法定量。结果表明,14种苯并咪唑类药物及其代谢物标准溶液在10~1 000 ng/mL的浓度范围内呈现良好的线性关系,R2均大于0.99,方法检测限为5μg/kg,定量限为10μg/kg,14种苯并咪唑类药物及其代谢物在10~200μg/kg添加浓度范围内回收率均70%~120%之间。批内、批间相对标准偏差均小于20%。本方法灵敏、准确,满足食品安全检测法规的要求。 相似文献
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建立了动物肌肉组织中阿苯达唑及其代谢物阿苯达唑亚砜、阿苯达唑砜的多残留检测的高效液相色谱法。用乙酸乙酯提取肌肉组织样品中的药物,提取液浓缩后加入酸性乙醇和正己烷分配脱脂,过watersHLB固相萃取柱净化。以水-乙腈-甲酸为流动相,反相高效液相色谱紫外检测法检测。方法的平均回收率为86．4％,平均变异系数为1．96％。3种药物的检测限均为5μg／kg,定量限均为20μg／kg。 相似文献
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高效液相色谱-串联质谱法检测猪肝中苯并咪唑类药物及其代谢物残留 总被引：1，自引：0，他引：1
建立了猪肝中阿苯哒唑及其代谢物阿苯哒唑砜、阿苯哒唑亚砜、2-氨基阿苯哒唑砜,噻苯哒唑及其代谢物5-羟基噻苯哒唑,甲苯哒唑及其代谢物氨基甲苯哒唑、5-羟基甲苯哒唑,芬苯哒唑及其代谢物芬苯哒唑砜、等效物奥芬哒唑,氟苯哒唑及其代谢物2-氨基-氟苯哒唑共14种苯并咪唑类药物及其代谢物残留检测的高效液相色谱-串联质谱方法。样品用乙酸乙酯提取,MCX固相萃取柱净化。WatersXterraC18色谱柱（2．1mm×150mm,5μm）分离,流动相A相为0．1％甲酸乙腈溶液;B相为0．1％甲酸水溶液,梯度洗脱,外标法定量。结果表明,14种苯并咪唑类药物及其代谢物标准溶液在10～1000ng／mL的浓度范围内呈现良好的线性关系,尺。均大于0．99,方法检测限为5斗g／kg,定量限为10μg／kg,14种苯并咪唑类药物及其代谢物在10—200μg／kg添加浓度范围内回收率均70％～120％之间。批内、批间相对标准偏差均小于20％。本方法灵敏、准确,满足食品安全检测法规的要求。 相似文献
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《蚕业科学》2015,(1)
将苯并咪唑类药物阿苯达唑喷施桑树后采叶喂蚕可预防和治疗家蚕微粒子病。准确测定与分析阿苯达唑在桑叶中的持留量及消解规律,对明确药物时效性以及确定施药方案十分重要。建立了固相萃取-高效液相色谱(SPE-HPLC)联用技术测定桑叶中阿苯达唑持留量的方法:桑叶样品中的药物成分用乙腈提取,经固相萃取氨基柱净化后,用高效液相色谱分离,再用二极管阵列检测器(DAD)检测以及外标法定量。方法学验证结果表明:药物浓度检测标准曲线相关系数R2=0.999 1;日内和日间的精密度良好,色谱峰保留时间的变异系数(RSD)为1.33%~3.88%,峰面积的RSD为2.40%~5.69%;样品回收率在80.67%~108.30%;最低检测限(LOD)和最低定量限(LOQ)分别为2.43 ng/L、8.09 ng/L。利用该方法对2次试验中分别以1 000 mg/L和1 500 mg/L阿苯达唑悬浊液喷施后的桑叶样品进行检测分析,各浓度药液处理区桑叶中的药物消解规律均符合一级动力学方程,半衰期在2.22~3.22 d。该检测方法具有准确、灵敏、快速的特点,可应用于桑叶中阿苯达唑持留量及消解规律的检测分析。 相似文献
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试验旨在建立饲料中万古霉素的超高效液相色谱—串联质谱检测方法,以0.05 mol/L磷酸盐缓冲液—乙腈(85:15,V/V)提取样品中的万古霉素,提取液经过强阳离子交换固相萃取柱净化,采用液相色谱—串联质谱多反应监测模式进行测定,外标法定量。不同饲料基质中,万古霉素在25~1000 ng/mL浓度范围内呈良好的线性关系。本方法对万古霉素在饲料中的检测限为0.05 mg/kg,定量限为0.1 mg/kg。在不同饲料中添加浓度范围为0.1~100 mg/kg,其平均回收率为86.7%~117.1%,批内变异系数在1.0%~17.4%之间,批间变异系数在2.9%~10.1%之间。本方法分析速度快、灵敏度高、重现性好,各项技术指标均满足国内外相关法规要求,可用于饲料中万古霉素含量的测定。 相似文献
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建立了高效液相色谱法测定预混合饲料、配合饲料和浓缩饲料中艾司唑仑含量的检测方法。试样用正己烷-二氯甲烷(1∶1,V/V)提取,经MCX固相萃取柱进一步净化,采用反相高效液相色谱-紫外检测法进行定量分析。饲料中艾司唑仑的检测限为0.1 mg/kg,定量限为0.25 mg/kg。在饲料中添加0.25、0.5、2.5 mg/kg水平的药物回收试验中,平均回收率均在82.8%~105.9%之间,相对标准偏差为1.62%~8.40%。该方法灵敏、准确,适用于饲料中艾司唑仑含量的分析检测。 相似文献
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牛肉中阿苯达唑及其代谢产物残留的检测 总被引：2，自引：0，他引：2
阿苯达唑是一种广谱抗蠕虫药,但是由于用药不当等原因,其在动物可食性组织中的残留可能刺激神经系统及消化系统、触发过敏反应甚至出现肝功能异常。本试验基于5μg/kg的检验限建立牛肉中的阿苯达唑及其代谢产物的检测方法——高效液相色谱法。样品中残留的阿苯达唑及其主要代谢产物阿苯达唑砜和阿苯达唑亚砜,经乙醚提取,乙腈和己烷萃取,由高效液相色谱（配紫外检测器）测定得到阿苯达唑及其亚砜与砜的平均回收率分别为71.8%～88.6%、78.1%～85.6%和83.1%～91.9%,变异系数分别为2.8%～9.2%、0.9%～4.9%和1.4%～4.8%。 相似文献
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建立高效液相色谱荧光检测法测定牛羊组织中碘醚柳胺残留量。牛羊组织中残留的碘醚柳胺,经乙腈-丙酮溶液提取,阴离子交换固相萃取柱净化,高效液相色谱-荧光法测定,外标法定量。碘醚柳胺在10～5000 ng/mL的浓度范围内呈现良好的线性关系,碘醚柳胺在牛羊肌肉、肝脏、肾脏、脂肪中的检测限均为2.5 μg/kg,定量限均为5 μg/kg,牛羊肌肉、肝脏、肾脏、脂肪中15～500 ng/g添加浓度范围内的回收率为77.4%～93.8%,批内批间RSD值均<15％。 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2％的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。 相似文献
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