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1.
Infected barks of chestnut blight cankers, caused by Cryphonectria parasitica , were collected from a naturally infected orchard and incubated at different temperatures. Cankers started to discharge ascospores about a week after incubation at 15–25°C; most ascospores were collected at 20 and 25°C. When incubated at 5, 10 or 30°C, only a few cankers released a small number of ascospores and only during the later stages of incubation. However, the rate of formation of perithecia was not affected by the incubation temperature. The number of airborne ascospores was monitored using a volumetric spore trap in a chestnut orchard during 1996 and 1997. In both years, the number of ascospores trapped daily varied greatly, but in general it increased sharply from March onwards, reached a peak in May, and then declined steeply. There was a significant correlation between daily counts of ascospores and air temperature. Time-series transfer function (TF) analysis showed a positive association of the daily number of ascospores with increasing temperature, rain events and wet/humid conditions. In general, values predicted by the TF model agreed well with the observed pattern. However, a multiple regression equation based on TF analysis failed to provide a satisfactory prediction of the daily number of ascospores.  相似文献   

2.
ABSTRACT Conventional models for the durability of resistant cultivars focus on the dynamics of the frequency of resistance genes. This leads to a definition of the durability of resistance as the time from introduction of the cultivar to the time when the frequency of the virulence gene reaches a preset threshold. It is questionable whether this is the most appropriate way to measure durability. Here we use a simple epidemiological model to link population dynamics and population genetics to compare three measures of durability: (i) the expected time until invasion of the virulent genotype, by mutation or immigration, and subsequent establishment of a population (T(invasion)); (ii) the virulence frequency related measure of the time for the virulent genotype to take-over the pathogen population ( T(take-over)); and (iii) the additional yield, measured by the additional number of uninfected host growth days (T(additional)). Specifically, we show how the measures of durability are affected by deployment and epidemiological parameters. We use a combination of numerical solution and analytical approximation of a model for the population dynamics of avirulent and virulent genotypes of a pathogen growing in dynamically changing populations of resistant and susceptible cultivars. The three measures of durability are compared. Some consequences of the results for durable resistance in multilines and mixtures and the regional deployment of resistant cultivars are discussed.  相似文献   

3.
塔克拉玛干沙漠腹地人工绿地三种灌木的离子吸收特性   总被引：6，自引：1，他引：5  
塔克拉玛干沙漠腹地人工绿地长期使用矿化度 4.0g/L以上的地下水灌溉 ,柽柳、梭梭和沙拐枣三种灌木仍然能够正常生长 ,表现出较强的抗盐特性。通过对三种不同年龄的灌木及其根、茎和凋落物的盐分分析 ,表明 :不同种类和年龄灌木对可溶性离子的吸收特性存在较大的差异 ;同一种灌木不同器官的离子吸收特性差异显著 ,表现出三种灌木在抗盐机理方面不同的生理和生态学特性 ,即 :柽柳从土壤中吸收的大量盐分离子 ,通过叶片的泌盐腺排出体外 ,使植物体内的盐分离子含量保持在相对较低的水平 ,这类植物属于泌盐类植物 ;梭梭能够从外界环境吸收大量的盐分 ,在植物体内储藏 ,并且依靠植物肉质同化枝吸收大量的水分 ,使得植物体内的盐分浓度维持在一定的限度内 ,这类植物属于盐多汁类植物 ;而沙拐枣通过根部运输系统的超滤 ,使得过量的盐被阻止在体外 ,保持植物体内较低的盐分浓度 ,此类植物主动避开盐分 ,属于拒盐类植物  相似文献   

4.
Intact spinach chloroplasts were used to determine if clomazone, 5-OH clomazone, and/or 5-keto clomazone inhibited the chloroplastic isoprenoid pathway. When isopentenyl pyrophosphate was used as a precursor, neither clomazone nor the clomazone metabolites (5-OH clomazone and 5-keto clomazone) inhibited the formation of products separated by HPLC in the organic phase. However, when pyruvate, a substrate for the first committed step of the pathway, was used as a precursor, both 5-keto clomazone and fosmidomycin reduced the formation of a non-polar product and increased the formation of a polar product in the organic phase. Only 5-keto clomazone, not 5-OH clomazone or clomazone, inhibited the formation of an additional product other than fosmidomycin in the aqueous phase from pyruvate incorporation. In an in vitro assay, 5-keto clomazone inhibited DXP synthase, the enzyme catalyzing the first committed step of the chloroplastic isoprenoid pathway. Therefore, our studies show that neither clomazone nor 5-OH clomazone inhibits the chloroplastic isoprenoid pathway, only 5-keto clomazone does.  相似文献   

5.
The neuropeptide AF2 has a complex set of actions on the dorsal muscle strip of Ascaris suum, including a potentiation of the acetylcholine-stimulated muscle contraction. Caffeine at 100 μm and 5 mm inhibited this potentiation, as did 100 μm theophylline in two out of six studies. The cyclic-AMP-potentiating compounds IBMX, dibutyryl cAMP and forskolin had no effect on the AF2-induced potentiation of the acetylcholine-stimulated muscle contraction. These preliminary data suggest that the potentiating action of AF2 is not mediated by a cAMP pathway.  相似文献   

6.
利用河西走廊东部5个气象站1961—2007年近47 a的逐月积雪深度和积雪日数的常规观测资料,分析河西走廊东部最大积雪深度和积雪日数月、季节和年的空间分布以及时间变化特征。结果表明:受海拔高度、地理位置以及天气系统等影响,河西走廊东部积雪日数、最大平均积雪深度和极端最大积雪深度的分布从东南向西北递减,高海拔地区多于低海拔区,山区多于平原区。时间变化上,积雪日数47 a来呈平稳变化趋势,震荡减少,变率很小,积雪深度呈增加趋势;河西走廊东部积雪日数最多出现在1月,最少出现在9月,积雪深度最大出现在3月,最小出现在12月;积雪日数冬季最大,春季次之,秋季最小;积雪深度春季最大,秋季次之,冬季最小;相关系数法分析表明,积雪日数和积雪深度都是冬季的变化主导了年尺度的变化;河西走廊东部积雪日数1990s中期以前以6~7 a、9~10 a和2~3 a的周期为主,1990s中期以后周期主要表现为6~7 a和2~3 a;积雪深度1970s中期以前以6~7 a和2~3 a周期为主,1970s中期~1990s中期9~10 a、12~13 a和2~3 a反应明显,1990s中期以后周期又表现为6~7 a和2~3 a。  相似文献   

7.
Ceratocystis platani is a quarantine fungal pathogen agent of canker stain, a destructive disease affecting Platanus. Despite its diagnosis being critical for disease control, there is still no effective diagnostic tool as all known mycological and biological detection assays are problematical. In this study we developed highly effective Real-Time PCR methods based on the use of an intercalating dye, EvaGreen, and on a Taqman probe. We designed primers and probe on the internal transcribed spacer (ITS) region of rDNA, and used them for the amplification and detection of a 95?bp C. platani amplicon. Inference of standard curves revealed that both Real-Time procedures have similar and high values of amplification efficiency when applied to a range of templates, e.g. genomic fungal DNA and DNA extracted from diseased wood. The methods were sensitive with a detection limit of 10?fg???l?1? C. platani genomic DNA. They were specific as they did not yield any detection signal when applied to non-target fungal taxa colonizers of Platanus wood. Reliability was demonstrated through the positive detection of a collection of C. platani isolates and of wood samples collected from naturally infected trees. Robustness was positively verified through detection on artificially infected trees, which were tested at different times after death, up to 27?months. Generating a standard curve with a target-amplicon-containing plasmid enabled an absolute quantification and a comparison between the discoloured wood of resistant and susceptible genotypes. The importance of the methods for studies on pathogen epidemiology and host resistance is also discussed.  相似文献   

8.
Brown JK 《Phytopathology》2000,90(4):320-323
ABSTRACT A method of estimating the frequency of recombination in field populations of ascomycete fungi is presented. This is a development of a technique described by J. Zhan, C. C. Mundt, and B. A. McDonald, in which field plots were inoculated with isolates of Mycosphaerella graminicola of known genotypes and then, later, the proportions of the fungal populations in the plots that originated as recombinant progeny of matings between the inoculated isolates or as immigrants from the local population were estimated. It is argued that J. Zhan, C. C. Mundt, and B. A. McDonald's method is inappropriate in terms of its approach to statistical inference and that the lack of confidence intervals for rates of recombination (r) or immigration (m) is a further weakness. An improvement to their method would involve the use of inoculated isolates that can be clearly distinguished from the local population, for instance, by the use of DNA markers. There should also be sufficient markers for there to be a negligible probability that progeny of matings between inoculated isolates will have the same genotype as either of their parents. With this experimental design, estimates of r and m can be found by a standard maximum likelihood method, while confidence intervals for the parameters can be estimated by a simple bootstrap procedure. The method appears to be about an order of magnitude more efficient than are the existing ways of estimating the frequency of recombination in ascomycete fungi.  相似文献   

9.
Addition of live mycelium ofRhizoctonia solani to three slightly, acid sands collected in winter from a field after a previous potato crop resulted in accumulation of hyperparasites and less infestation of sprouts of infected seed potatoes. A similar effect was observed in alkaline marine sandy loam soils, but only after a second addition of live mycelium. The predominant hyperparasite in these experiments wasVerticillium biguttatum.  相似文献   

10.
In a gnotobiotic piglet infected with 50.10(6) oocysts of Cryptosporidium parvum, developmental stages of the parasite were found in the duodenum, anterior jejunum and middle jejunum on the first day post infection (1 DPI). During the studies of the ultrastructure, trophozoites and meronts of C. parvum were encountered in the microvillous zone of enterocytes, and the outer membrane of parasites was a continuation of the membrane of microvilli. Unusual was the attachment of C. parvum trophozoite on the opening of a goblet cell, as well as a meront localized in the cytoplasm in the enterocyte of anterior jejunum. These findings show that C. parvum is very adaptive and capable of developing in a still undescribed location.  相似文献   

11.
张掖绿洲冷岛效应时空格局的遥感分析   总被引：3，自引：0，他引：3  
地表温度是生态系统中极其重要的环境因子,中小尺度区域的热量资源和热场分布对绿洲冷岛效应的研究以及评价绿洲的稳定性具有重要意义。利用LandsatTM卫星影像,采用单窗算法,反演了张掖绿洲1999年和2007年两个时期的陆地表面温度(LST),构建了绿洲"冷岛"比例指数,定量分析了张掖地区近8年来绿洲冷岛效应的时序变化,并且通过对张掖热场的分析,揭示了冷岛效应的空间变化特点。结果表明:①张掖绿洲冷岛效应明显,但近8年来有减弱的趋势;②南北向绿洲内部和荒漠内部温度差异均在减小,城区温差在扩大;东西向绿洲内部和城区内部温度差异均在减小,荒漠温差在扩大;③水体、耕地、草地、建设用地和林地的平均LST有所降低,荒漠等未利用土地LST有所升高,其中建筑用地的LST减幅最大。为研究绿洲的光、热、水、土的空间分布及区域变化,评价绿洲的稳定性提供了一种新的角度,同时对绿洲与荒漠之间的大气物理模型及动力学有一定参考意义。  相似文献   

12.
Thirty-six isolates of the fasciation-inducing bacterium Rhodococcus fascians were evaluated for the presence and location of the fas-1 gene, which codes for an isopentenyl transferase, the committed step in cytokinin biosynthesis. The presence of fas-1 was determined by PCR using a set of primers to the most conserved regions of the gene and by Southern hybridization to genomic digests using the PCR fragment as a probe. Both methods revealed the presence of the gene in 18 virulent isolates and the absence of the gene in 18 avirulent isolates. Thus, there is a strong relationship between the presence of the gene and virulence of the organism. The location of fas-1 was determined by probing blots of linear and circular DNA. For most of the virulent isolates, the gene was localized to a 200 ± 10 kb linear plasmid. Three virulent isolates lacked a plasmid of this size, but contained fas-1 either on a linear plasmid of 130 kb or on a large circular plasmid.  相似文献   

13.
Leaf mesophyll protoplasts and cell suspension cultures obtained from resistant and susceptible cultivars of hop ( Humulus lupulus ) exhibited differential cell death responses when treated with sterile culture filtrates (CF) obtained from four isolates of Verticillium albo-atrum showing a range of virulence towards hop. The extent of cytotoxicity was correlated with both the virulence of the isolates and the resistance of the cultivars. Cytotoxicity was attributed to a low-molecular-weight, heat-labile, component of CF. Cell cultures from a susceptible and a resistant cultivar exhibited similar, growth-related, patterns of sensitivity to CF. The extent of inhibition of callus growth caused by CF treatment was related to the level of the resistance of the cultivar.  相似文献   

14.
The pathogenicity-related traits of biotrophic plant pathogens are usually measured on the individual host plant, at the scale of a single pathogen life cycle, whereas epidemic development in the field encompasses a succession of cycles. It remains unclear which traits make the greatest contribution to pathogen fitness in the field and to epidemic severity. The objective of this study was to determine the contributions of elementary pathogenicity traits to epidemic development in field conditions. We challenged a set of wheat cultivars with three different leaf rust isolates, under both controlled and field conditions, in 3 consecutive years. Infection efficiency, latent period, lesion size, spore production per lesion and spore production capacity were measured in the greenhouse, whereas disease severity was measured in the field. Most, but not all, of the pathogenicity traits were related to each other. All traits contributed to epidemic development in the field, but to different extents. Surprisingly, lesion size and spore production per lesion were inversely correlated with epidemic severity. Conversely, there was a strong positive correlation between spore production capacity and pathogen fitness in the field, in accordance with the concept of propagule pressure as a strong determinant of invasion success. Severe epidemics were mostly associated with small lesions with a high spore production capacity, high infection efficiency and a short latent period.  相似文献   

15.
The carnations which are normally grown are practically all infected with various viruses, and therefore attempts were made to obtain virus-free plants of a number of varieties by means of heat treatment and meristem culture. Incubators were designed in which the plants could be subjected to a heat treatment at a temperature of 37°C and at a high degree of humidity of the air. The ultimate percentage of virus-free plants in 1960 amounted to an average of 1.3%. This is sufficient to obtain a virus-free clone, but not enough to build up a virus-free assortment within a short time. The factors which may affect the percentage of successful isolations are discussed, viz. the effect of the season, of varietal differences and of differences between the material supplied by different growers. When the virus-free plants are propagated in practice it is essential that the material should be closely inspected and tested at regular intervals. The new material was found to possess most favourable qualities. It is sold with a special certificate.  相似文献   

16.
Clubroot (Plasmodiophora brassicae) is an important disease of canola (Brassica napus) and other brassica crops. Accurate estimation of inoculum load in soil is important for evaluating producer risk in planting a susceptible crop, but also for evaluation of management practices such as crop rotation. This study compared five molecular techniques for estimating P. brassicae resting spores in soil: quantitative polymerase chain reaction (qPCR), competitive positive internal control PCR (CPIC-PCR), propidium monoazide PCR (PMA-PCR), droplet digital PCR (ddPCR) and loop-mediated isothermal DNA amplification (LAMP). For ddPCR and LAMP, calibrations were developed using spiked soil samples. The comparison was carried out using soil samples collected from a long-term rotation study at Normandin, Québec, with replicated plots representing 0-, 1-, 2-, 3-, 5- and 6-year breaks following susceptible canola infested with clubroot. CPIC-PCR and ddPCR provided repeatable estimates of resting spore numbers in soil compared with estimates from qPCR or LAMP alone. CPIC-PCR provided the most robust measurement of spore concentration, especially in the 2 years following a crop of susceptible canola, because it corrected for effects of PCR inhibitors. PMA-PCR demonstrated that a large proportion of the DNA of P. brassicae detected in soil after the susceptible canola crop was derived from spores that were immature or otherwise not viable. Each assay provided a similar pattern of spore concentration in soil, which supported the conclusion of a previous study at this site that resting spore numbers declined rapidly in the first 2 years after a susceptible crop, but much more slowly subsequently.  相似文献   

17.
Time- and concentration-course studies were conducted to determine the effect of thirteen herbicides on photosynthesis, respiration, RNA synthesis, protein synthesis, and lipid synthesis using isolated single leaf cells. Each herbicide was from a different chemical class. Appropriate 14C-substrates and product purification procedures were used for each process prior to liquid scintillation counting. The most sensitive metabolic site of inhibition was photosynthesis for atrazine, bromacil, dichlobenil, monuron, and paraquat; RNA synthesis for dalapon and dinoseb; protein synthesis for chlorpropham; and lipid synthesis for CDAA, chloramben, 2,4-D, EPTC, and trifluralin. However, with several herbicides, one or more process was almost as sensitive as the one mentioned above. All herbicides inhibited more than one process, and the most sensitive site of inhibition may not be the same process that was inhibited the greatest at the maximum concentration and maximum exposure time used. Therefore, a concept of metabolic sites of action, rather than a primary site of action, appears to be more meaningful for herbicides.  相似文献   

18.
Chloroneb (1,4-dichloro-2,5-dimethoxybenzene) is a fungicide with moderate systemic properties and selective activity toward certain fungi (e.g., Phytophthora, Pythium, Botrytis, Mucor). Although the compound is metabolized by some fungi to the toxic oxidation products DCMP (2,5-dichloro-4-methoxyphenol) and DCHQ (2,5-dichlorohydroquinone) the fungitoxic principle is chloroneb per se. It causes a lysis of the inner mitochondrial membrane of sensitive fungi, attacks the nuclear membrane and also other membranes at higher doses. Oxygen consumption by isolated mitochondria slowly decreases, but the respiratory control quotient remains nearly constant. No specific uncoupling activity or inhibition of the respiratory chain could be detected. Chloroneb is bound to the mitochondrial membrane in sensitive organisms, and a good correlation exists between binding capacity, damage to mitochondrial structure and function, and growth inhibitory effects. DCMP has a nearly identical effect, but some additional uncoupling activity whereas DCHQ is different in its mechanism of action. A comparison of a chloroneb-sensitive strain of Mucor mucedo and a resistant strain with respect to the amino acid composition of their mitochondrial membranes demonstrated a significantly diminished content of tyrosine in the resistant strain, which may be associated with the decreased binding capacity for chloroneb. Although the reason for mitochondrial destruction is not yet elucidated, counteracting effects of piperonylbutoxide and α-tocopherol acetate suggest that a radical mechanism may be involved.  相似文献   

19.
Imines, synthesised by the reaction of sec-butylamine (SBA, 2-amino-butane) with pentan-2-one, heptan-2-one, octan-2-one, undecan-2-one, benzaldehyde and 2-furaldehyde, were evaluated as vapour-phase treatments to prevent the infection of oranges by Penicillium digitatum. All the imines provided decay control equivalent to SBA when they were introduced into the fruit container at a rate equivalent to 16.4 mg SBA litre?1 air volume. The carbonyl compound component of the imines, tested alone at a five-fold higher rate, did not reduce fruit decay. All the imines were stable in the gaseous state in dry air. The imines of pentan-2-one and undecan-2-one were hydrolysed rapidly in a humid environment, indicating that their action against P. digitatum was due to the release of SBA into the atmosphere around the fruit. The instability of the imines of the aliphatic ketones makes them unsuitable as generators of SBA in packages. The imines derived from benzaldehyde and 2-furaldehyde were stable in moist air, but were hydrolysed to a significant degree on moist filter paper, especially at pH < 7. These aromatic imines were absorbed from the atmosphere by the fruit and were hydrolysed rapidly in water, and in a buffer solution that represented the environment in fresh injuries on fruit. Apparently all the imines tested generated SBA in the fruit environment, and this SBA accumulated at potential infection sites, where it prevented the development of decay.  相似文献   

20.
BACKGROUND: Aiming at the rational design of new herbicides, the availability of the three‐dimensional structure of the target enzyme greatly enhances the optimisation of lead compounds and the design of derivatives with increased activity. Among the most widely exploited herbicide targets is glutamine synthetase. Recently, the structure of a cytosolic form of the maize enzyme has been described, making it possible to verify whether steric, electronic and hydrophobic features of a compound are in agreement with inhibitor–protein interaction geometry. RESULTS: Three series of compounds (aminophosphonates, hydroxyphosphonates and aminomethylenebisphosphonates) were evaluated as possible inhibitors of maize glutamine synthetase. Aminomethylenebisphosphonate derivatives substituted in the phenyl ring retained the inhibitory potential, whereas variations in the scaffold, i.e. the replacement of the second phosphonate moiety with a hydroxyl or an amino residue, resulted in a significant loss of activity. A kinetic characterisation showed a non‐competitive mechanism against glutamate and an uncompetitive mechanism against ATP. A docking analysis suggested the mode of bisphosphonate binding to the active site. CONCLUSION: Results made it possible to define the features required to maintain or enhance the biological activity of these compounds, which represent lead structures to be further exploited for the design of new substances endowed with herbicidal activity. Copyright © 2009 Society of Chemical Industry  相似文献   

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