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1.
Using observations from 38 ichthyoplankton surveys conducted near Shelikof Strait, Alaska between 1979 and 1992, we characterized the horizontal distribution and spatial patchiness of the early life stages of walleye pollock ( Theragra chalcogtamma ). Lloyd's index of patchiness ranged from 3.9-6.1 for eggs and 3.9–16.2 for larvae. This index was size (age) dependent: low for eggs, high for newly hatched larvae, then decreasing through late larval stage. By the early juvenile stage, patchiness increased as pollock began to school. The percentage of larvae in a patch (defined as the percentage of larvae present at stations where larval counts exceeded the mean by one standard deviation during the given survey) varied greatly (26–92%). Larval distributions were used to deduce physical mechanisms responsible for patches. Three categories of patches were identified: those created by interaction of larvae with time-dependent currents, those in the vicinity of Sutwik Island, and those associated with eddies. Simulation experiments were utilized to examine processes influencing patch formation and the role of larval swimming. Between 5 and 6 weeks after hatching, larvae have swimming abilities that enable them to maintain a patch already created by physical mechanisms.  相似文献   

2.
The occurrence and density of Pacific saury Cololabis saira larvae and juveniles were examined in relation to environmental factors during the winter spawning season in the Kuroshio Current system, based on samples from extensive surveys off the Pacific coast of Japan in 2003–2012. Dense distributions of larvae and juveniles were observed in areas around and on the offshore side of the Kuroshio axis except during a large Kuroshio meander year (2005). The relationships of larval and juvenile occurrence and density given the occurrence to sea surface temperature (SST), salinity (SSS), and chlorophyll‐a concentration (CHL) were examined by generalized additive models for 10‐mm size classes up to 40 mm. In general, the optimal SST for larval and juvenile occurrence and density given the occurrence was consistently observed at 19–20°C. The patterns were more complex for SSS, but a peak in occurrence was observed at 34.75–34.80. In contrast, there were negative relationships of occurrence and density given the occurrence to CHL. These patterns tended to be consistent among different size classes, although the patterns differed for the smallest size class depending on environmental factors. Synthetically, the window for spawning and larval and juvenile occurrence and density seems to be largely determined by physical factors, in particular temperature. The environmental conditions which larvae and juveniles encounter would be maintained while they are transported. The survival success under the physically favorable but food‐poor conditions of the Kuroshio Current system could be key to their recruitment success.  相似文献   

3.
Since there have been practically no surveys of the eggs of Pacific saury (Cololabis saira) in the western North Pacific (WNP), its spawning ground (SG) distribution has been poorly resolved, based mainly on the larval distribution. This means of estimating SG distribution is imprecise because saury eggs drift for more than a week before they hatch, in a region with intense western boundary currents and their extensions. To improve our understanding of the immature saury, a large number of larvae (body length <25 mm) collected in the WNP during 1993–96 were numerically backtracked to take into account the advection by geostrophic and wind‐forced Ekman currents, and the SG locations and ambient sea surface temperatures (SSTs) for the eggs and larvae on the backtracking trajectories were estimated. The resulting seasonal distributions of SGs indicated that both the locations and the intensities of spawning change from season to season. Moreover, the ambient SSTs for eggs just after fertilization ranged from a high of around 21.5°C in early autumn (September to October) to a low of around 15.0°C in late spring (May to June) with an intermediate of around 20.0°C in winter (January to February). The ambient SSTs showed seasonally different gradients while the individuals developed from eggs to early larvae: the SSTs decreased throughout the autumn (September to December), stayed rather constant in winter (January to February), and increased throughout the spring (March to June). The ambient SSTs for the early larvae were at around 19.0°C in autumn and winter (September to February) and around 16.5°C throughout the spring (March to June).  相似文献   

4.
Diel and ontogenetic changes in larval body density related to swim bladder volume were investigated in Pacific bluefin tuna, Thunnus orientalis, to determine the causality of larval mortality – adhesion to the water surface and contact with the tank bottom during seedling production. The density of larvae with deflated swim bladders increased with total length and days post hatch. Diel density change was observed after day 2 post hatch; owing to daytime deflation and night‐time inflation of the swim bladder, the density was relatively higher during the daytime. Increased swim bladder volumes clearly reduced larval density during the night‐time after day 9 post hatch. However, the density of larvae with inflated swim bladders was greater than rearing water density (Δρ>0.0099). The small density difference between larvae and rearing water (Δρ=0.0022?0.0100) until day 4 post hatch may have caused larval mortality by adhesion to the water surface because larvae can be easily transported to the water surface by aeration‐driven upwelling in rearing tanks. Density increased noticeably from day 5 to day 9 post hatch. The increased density difference (Δρ=0.0065?0.0209) in larvae and rearing water possibly induced mortality by contact with the tank bottom because larvae sink particularly during the night‐time on ceasing swimming.  相似文献   

5.
The role of behavior, especially vertical migration, is recognized as a critical component of realistic models of larval fish dispersion. Unfortunately, our understanding of these behaviors lags well behind our ability to construct three-dimensional flow-field models. Previous field studies of vertical behavior of larval Pacific cod ( Gadus macrocephalus ) were limited to small, preflexion stages (≤11 mm SL) in a narrow range of thermal conditions. To develop a more complete picture of larval behavior, we examined the effects of ontogeny, temperature, and light on vertical responses of larval Pacific cod in experimental columns. While eggs were strictly demersal, yolk-sac larvae displayed a strong surface orientation as early as 1 day post hatch (∼ 5 mm SL). Consistent with field observations, small preflexion larvae (<10 mm SL) showed no response to varying light levels. However, there was a direct effect of temperature on larval behavior: Pacific cod larvae exhibited a stronger surface orientation at 4°C than at 8°C. The behavior of larger, postflexion larvae (>15 mm SL) in experimental columns was consistent with a diel vertical migration and independent of water temperature: fish were more widely distributed in the column, and median positions were consistently deeper at higher light levels. These laboratory observations are combined with observations from discrete-depth (MOCNESS) sampling in the Gulf of Alaska to characterize the vertical distribution of larval Pacific cod and contrast ontogenetic patterns with walleye pollock ( Theragra chalcogramma ). The vertical movements of larval Pacific cod described here will be applied in the development of dispersal projections from Gulf of Alaska spawning grounds.  相似文献   

6.
ABSTRACT:   To investigate the changes in maternal effects during early development in Japanese flounder Paralichthys olivaceus , four-by-four factorial mating by artificial fertilization was performed to produce 16 families. Larvae and juveniles of each family were reared under common environmental conditions until completion of metamorphosis. The magnitude of maternal effects was estimated as the differences between sire and dam variance. Maternal effects were highest at hatching, and then disappeared at 30 days after hatching. During early development, larval size at hatching was largely affected by egg size, but this effect decreased as larvae grew. Estimated mean heritability during early development was nearly constant (0.14 ± 0.06 for total length and 0.10 ± 0.05 for body depth). Therefore, it is suggested that early development in Japanese flounder is affected by both maternal effect and genetic factors.  相似文献   

7.
Pinfish (Lagodon rhomboides Linnaeus) and pigfish (Orthopristis chrysoptera L.) are relatively new, cultured species commonly used as marine baitfish in the south‐eastern United States and currently have no defined protocols for egg incubation. Thus, experiments were conducted to determine efficient egg stocking densities during static and flow‐through incubation that yielded higher quality larvae. Eggs of each species were incubated statically at 250, 500, 1000 and 2000 eggs L?1, and after incubation, egg hatching success, larval survival to first feeding, larval morphometrics and water quality were assessed. Stocking densities above 250 eggs L?1 led to significant reductions in pinfish hatching success and water quality degradation, evident from decreased dissolved oxygen and pH levels and increased nitrogenous wastes. Increased stocking densities for pigfish also resulted in significant water quality degradation, although hatching success was unaffected up to a density of 1000 eggs L?1. A high flow‐through water exchange rate of 2000% daily resulted in significant reductions in nitrogenous wastes and greater stability in dissolved oxygen and pH levels during incubation when compared to static treatments of the same egg density. Additionally, the high exchange rate of 2000% was critical in maintaining high hatching success and larval survival to first feeding at stocking densities of 1000 eggs L?1 for pinfish and up to 4000 eggs L?1 for pigfish. No clear patterns in larval morphometrics were observed among stocking densities. Static incubation densities of 250 and 1000 eggs L?1 are recommended for pinfish and pigfish, respectively.  相似文献   

8.
The growth, survival, food selection and consumption of pompano larvae under different rotifer densities as well as their colour preference during the rotifer feeding stage were examined in this study. Growth and survival of fish larvae were not significantly affected when rotifer density was between 10 and 20 mL?1. Fish larvae grew slower at 1 and 40 rotifers mL?1 than at 10 and 20 rotifers mL?1, and higher fish survival was achieved when fish larvae were exposed to 10 and 20 rotifers mL?1. The rotifer density of 1 mL?1 not only reduced food ingestion during the early stage, but also delayed diet switch from rotifer to copepod nauplii. On 5 days post hatching (DPH), larval pompano ingested more rotifers in dark‐coloured tanks and ingested more rotifers when prey colour was green. Based on the results obtained in the present study, the culture of larval pompano larvae is recommended using dark wall tanks with a feeding density of 10–20 rotifers mL?1 during the initial feeding stage. This study proposes a management protocol to use appropriate type and quantity of live food to feed pompano larvae in a hatchery rearing condition, which could be applicable to the culture of fish larvae in other marine fish species.  相似文献   

9.
Abstract.— Nutritional condition in larval fish culture has generally been evaluated based on growth and survival, and stress tolerance by air exposure. Here we made direct observations of fish in rearing tanks to estimate the quality of the fish. Pacific threadfin Polydactylu.s sexfilis larvae were reared with rotifers and Artemia nauplii enriched with three levels of highly unsaturated fatty acids (HUFA). The three levels were defined as no-, medium-, or high-HUFA groups, in which the fatty acids emulsions contained 0, 30, or 50%. respectively. of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). The formation of patchiness (dense aggregations of fish on the surface) and development of schooling behavior were observed in four replicate rearing tanks for each feed treatment. Patchiness was formed in every tank with the highest densities of 60–250 fish/L on day 7–8 posthatch. Both medium-HUFA and high-HUFA groups showed schooling behavior at 19 d after hatching, whereas fish in the no-HUFA group did not form schools even at day 25. The body lengths of fish from each group were not significantly different, although the no-HUFA group suffered high mortality after day 20. Our results suggest that HUFA deficiency has a serious negative impact on P. sexfilis during metamorphosis rather than during the early larval stage. We also consider that direct observation rather than stress tests by air exposure may be an effective tool to measure the quality of juvenile fish.  相似文献   

10.
Juvenile walleye pollock of the Japanese Pacific population were collected from the Funka Bay [spawning ground; 16–64 mm fork length (FL)] in spring and the Doto area (nursery ground; 70–146 mm FL) in summer. Hatch dates were estimated by subtracting the number of otolith daily increments from sampling dates, and their early growth was back‐calculated using otolith radius–somatic length relationships. Interannual change of the hatching period was observed during 2000–02, and the peaks ranged from mid‐February in 2000 to early‐April in 2002. In 2000, when a strong year class occurred, early life history of the surviving juveniles could be characterized by early hatching and slower growth in the larval stage (<22 mm length). Higher growth rate in 2001 and 2002 did not always lead to good survival and recruitment success. Even though their growth was slow in 2000, the larvae hatched early in the season had larger body size on a given date than faster‐growing larvae hatched in later season in 2001 and 2002. Bigger individuals at a certain moment may have advantage for survival. The delay of hatching period may result in higher size‐selective mortality, and as a necessary consequence, back‐calculated growth in 2001 and 2002 could shift towards higher growth rate, although abundance of such a year class would be at the lower level. Variability in spawning period, early growth and their interaction might have a strong relation to larval survival through cumulative predation pressure or ontogenetic changes in food availability.  相似文献   

11.
Growth of Pacific halibut Hippoglossus stenolepis larvae was studied in the laboratory during 1989 and 1991. Larvae increased in length from 6.3 mm at hatching to 9.9 mm 20 d post-hatch. The average daily length increment was 0.17 mm. Dry weight of the larvae increased from an average of 210 μg at hatching to 570 μ g on day 20, providing a specific growth rate of 4.99. During the same period, mean yolk sac weight decreased from 1,390 μ g to 646 μ g, resulting in a yolk to body conversion efficiency of 48.5%. At hatching, the larval body made up only 13% of total dry weight. On day 20, the larval body made up 46.9% of the total weight. Larvae started feeding at a length of 12 mm after about 90% of their yolk sac had been absorbed.  相似文献   

12.
The effects of larval density, rearing media, and protein additions on fish larval survival were tested by rearing larvae in small containers. Yolk-sac larvae of Japanese flounder, Paralichthys olivaceus, were transferred 1 day after hatching to plastic dishes and cell-culture microplates by Pasture pipette at densities ranging from 0.3 to 30 individuals/ml and incubated at 16 °C without food. Dead larvae were removed and counted every 12 h. Three peaks of larval mortality were observed: the first peak appeared just after the transfer, the second corresponded to the completion of yolk absorption, and the third occurred 10–12 days after the transfer. Since about 50% of larvae survived until the third peak, it was clear that mortality at the second peak (completion of yolk absorption; equivalent to the first feeding) was not due to starvation. At the first mortality peak, better survival was unexpectedly recorded at higher larval densities (30 individuals/ml), suggesting that some material beneficial for survival is derived from the larvae. This idea was further supported by the improved survival in media previously used to rear larvae at high densities. The addition of bovine serum albumin (BSA) at 10 μg/ml effectively reduced mortality in microplates and in 30-l tanks. To reduce the larval mortality just after handling, addition of egg white at a dilution rate of 1/30,000 (vol/vol) or less is recommended as a practical alternative to BSA. Since the addition of polyethylene glycol 6000 (1 μg/ml) also effectively reduced mortality, reduction in mortality could not be attributed to a specific mechanism. The precise determination of the magnitude and timing of larval mortality was attained through the use of small containers, and a method to prevent mortality due to handling was identified in this study. These types of tests may allow the logical evaluation and establishment of better rearing procedures for seed production in aquaculture.  相似文献   

13.
Variability in growth rates of larval haddock in the northern North Sea   总被引:5,自引:0,他引:5  
The large-scale distribution of haddock ( Melanogrammus aeglefinus ) larvae in the northern North Sea was mapped in a grid survey carried out in late April 1996. A drifting buoy was deployed in the centre of one of the areas of concentration of larvae located off the east coast of the Shetland Isles, where intensive sampling was carried out for ≈ 10 days. Daily larval haddock growth variability, estimated from otolith microstructure analysis, was independent of the measured variability of the physical and biological environment of the larvae. The survey coincided with the onset of the spring plankton production bloom, and a likely explanation for the absence of environmental effects on larval growth was high food availability and larval feeding rates. Nevertheless, differences in growth were observed between cohorts, with larvae hatched later in the spring displaying higher growth at age than those hatched earlier. Particle-tracking modelling suggested that differences in temperature history between cohorts, on their own or compounded by a potential interaction between temperature and the development of plankton production, may explain the higher growth rate of the larvae hatched later in the season.  相似文献   

14.
The daily mortality rates of North Sea herring early‐stage larvae are found to vary over decades. Larval abundance data were used with a spatio‐temporal oceanographic model to reconstruct temperature histories of the observed larvae. The histories were used in conjunction with a temperature‐based growth model to estimate larval age. Mean daily mortality rates were then estimated for the four spawning components (Downs, Banks, Buchan and Orkney/Shetland) using the vertical life table approach, which considers instantaneous abundances across all ages rather than following distinct cohorts. All spawning components, but especially Downs (in the south), exhibited a steady rise in mortality associated with increasing population size. In addition, the three northern components shared a distinct trend in mortality that was significantly correlated with ambient water temperatures experienced by the larvae during the respective time periods after hatching. This trend was also significantly negatively correlated with the residuals of the whole stock‐recruitment relationship. These findings were generally robust to assumptions about growth and hatch length of larvae. The compensatory increase in productivity in the late 1980s and poor recruitment since 2000 coincide with changes in the mortality of larvae younger than 30 days post hatch and covary with larval density and temperature. Thus we suggest that the mortality of early‐stage larvae does impact on the population dynamics in North Sea herring in its current productivity regime, implying a critical period in the determination of year class strength.  相似文献   

15.
This work aimed to evaluate the relationship between morphological development, oxygen consumption and reduced mortality in larval fish. We measured the resting metabolic rate (RM), specific metabolic rate (SMR) and the change in the total length during the larval stage of four fish species. Resting metabolism decreased from hatching to mouth opening, and then increased after mouth opening. The changes in the SMR were variable during the larval stage. After hatching, there was no increase in SMR in yolk‐sac larvae. However, SMR increased between mouth opening and the onset of notochord flexion and then decreased during notochord flexion before finally stabilizing. We observed two peaks in mortality during the larval period of all species: between mouth opening to the onset of notochord flexion and from the completion of notochord flexion to the juvenile stage based on the per cent mortality and the number of dead fish collected from the bottom of the rearing tank. Interestingly, the changes in SMR coincided with these periods of mortality. We hypothesize that larvae require more energy during these periods of larval development and are thus more susceptible to mortality when energy is insufficient. Thus, it is important to supply enough nutrition to larvae in during early development to prevent mass larval mortality.  相似文献   

16.
ABSTRACT:   Lipovitellin (Lv), the major yolk protein derived from vitellogenin (Vg), was purified from vitellogenic ovaries of Pacific saury Cololabis saira using hydroxylapatite column chromatography followed by gel filtration. The apparent native mass of purified Lv was approximately 420 kDa, while the tertiary structure of Lv revealed by sodium dodecylsulfate–polyacrylamide gel electrophoresis was typical of teleost Lvs, consisting of a heavy chain (∼99 kDa) and a light chain (∼34 kDa). Western blot analysis using rabbit antiserum raised against Pacific saury Lv revealed a specific reaction with a polypeptide (∼194 kDa) that is present in serum from female Pacific saury but not in male serum, suggesting the approximately 194-kDa polypeptide to be the Vg monomer. This study describes the first step toward the development of specific immunoassays for Pacific saury Vg, which will be an effective tool for monitoring the reproductive development of this species.  相似文献   

17.
Recently, based on the histological studies it is suggested that Pacific bluefin tuna (PBF) spawns in the Kuroshio–Oyashio transition, off the Pacific coast of northeastern Japan, in addition to the previously identified two spawning areas of the northwestern Pacific around the Nansei Islands and the Sea of Japan. Distributional surveys for PBF larvae have not been conducted in the Kuroshio–Oyashio transition, and thus, we conducted PBF larval surveys at twenty stations in this area in early August 2018 to obtain evidence for the spawning of PBF. Twelve PBF larvae (3.9–7.2 mm in body length) were collected at six stations where sea surface temperature ranged from 27.2 to 28.2°C. Otolith microstructure analysis indicated that age of these larvae ranged from 4 to 11 days after hatching and the larvae hatched in late July. Growth of the collected larvae was comparable to those in the other two spawning grounds. Therefore, PBF spawns, hatches, and at least survives to the postflexion stage, 11 days after hatching in the Kuroshio–Oyashio transition. This fact potentially has a large impact on recruitment processes of PBF if they survive to recruitment in this third spawning ground.  相似文献   

18.
A fish bioenergetics model coupled with an ecosystem model was developed to reproduce the growth of Pacific saury. The model spatially covers three different oceanographic spatial domains corresponding to the Kuroshio, Oyashio, and interfrontal (mixed water) regions. In this coupled model, three (small, large, and predatory) zooplankton densities which were derived from the lower trophic level ecosystem model were input to the bioenergetics model of saury as the prey densities. Although certain model parameters were imposed from other species’ bioenergetics, several model parameters were estimated from observational data specific to Pacific saury. The integrated model results reproduced appropriate growth rates of Pacific saury. Model sensitivities to water temperature and prey density are examined and observational methods to evaluate the model parameters are discussed.  相似文献   

19.
为了提高秋刀鱼(Cololabis saira)渔情预报模型的时空分辨率, 提升生产经济效益, 本研究基于 2013─2016 年 7—11 月中国在西北太平洋公海的秋刀鱼生产数据及海洋环境数据, 利用广义可加模型(generalized additive models, GAM)分别拟合单位捕捞努力量渔获量(catch per unit effort, CPUE)的适宜性指数(suitability index, SI)与各海洋环境变量之间的 SI 模型, 结合提升回归树模型(boosting regression tree, BRT)进行权重分析, 建立以月份为周期的秋刀鱼栖息地适宜性指数(habitat suitability index, HSI)模型。结果表明, (1) GAM 能较好地拟合适宜性指数与环境变量的关系, 获得最优环境变量参数值;(2) 环境变量对 CPUE 影响权重的前 3 位分别为海表温度梯度、海表温度和混合层深度, 其中, 在秋季 9—11 月海表温度梯度的权重值均为最高;(3) HSI 模型的检验和评价总体准确率分别为 82.0%和 73.2%, 秋季可达 87.7%和 77.9%, 在盛渔期 10 月, 预测准确率达 89.4%;(4) HSI 高值区与秋刀鱼实际渔场在空间分布基本一致。研究表明该模型适用于秋刀鱼的渔情预报, 并在每天的速报中具有明显优势。  相似文献   

20.
This research examined the effect of initial stocking density and feeding regime on larval growth and survival of Japanese flounder, Paralichthys olivaceus. Larval rearing trials were conducted in nine 50‐L tanks with different initial stocking densities combined with different feed rations (20 larvae/L with standard feed ration [LD], 80 larvae/L with standard feed ration [HD], and 80 larvae/L with four times the standard feed ration [HD+]). Larvae were stocked on 0 days posthatch (DPH) following hatching of the fertilized embryos. Larval total length (TL), survival rates, and final densities were observed on larval settlement (32 DPH) to evaluate larval rearing performance. At 32 DPH, there were no significant differences (p > .05) in TL or survival rates between the LD (46.5 ± 17.0%) and HD+ (40.3 ± 9.4%). The TL and survival rate of HD (23.1 ± 3.5%) were significantly lower than that of LD and HD+ (p < .05). However, the larval density of HD was significantly higher than that of LD (p < .05). HD+ achieved the best larvae production (32.27 ± 7.51 larvae/L), supported by sufficient food source, high water exchange, and proper water quality management (routine siphoning, surface skimming). The larval‐rearing protocols and larval development from hatching to metamorphosis is described in detail, with corresponding photographs taken during the experiment.  相似文献   

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