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1.
为了解河南省肉鸡屠宰加工生产链中沙门菌血清型分布及耐药性,采用随机抽样的方法采集肉鸡屠宰加工厂的肉鸡样本,按照国家标准进行沙门菌的定性检测和血清型鉴定,通过微量肉汤稀释法对阳性分离株进行药敏试验。结果表明:554份肉鸡样本中检出沙门菌77份,其中肛拭子阳性率(32份)8.7%,肉鸡胴体阳性率(45份)24.2%。共鉴定出5种血清型,以印第安纳沙门菌和肠炎沙门菌为主。耐药性结果显示分离株对磺胺类、氨基糖苷类、喹诺酮类、β-内酰胺类、四环素类等均具有严重的多重耐药性,其中,喹诺酮类耐药性高达100.0%,耐药最小抑菌浓度分布主要集中在中高浓度区间。提示河南省肉鸡屠宰加工生产链中沙门菌污染以印第安纳沙门菌和肠炎沙门菌血清型为主,且存在较严重的耐药性,应从源头加强肉鸡孵育、养殖环节卫生控制,减少沙门菌的污染和抗菌药物的使用。  相似文献   

2.
为了解银川地区鸡源沙门菌的耐药性及优势血清型,采用药敏纸片扩散法测定了69株鸡源沙门菌对常用12种抗菌药物的耐药率,同时采用玻板凝集法对沙门菌进行了血清型鉴定。结果显示,所有菌株对12种抗菌药物均产生了不同程度的耐药性,其中对萘啶酸、复方新诺明、氨苄西林、头孢唑啉的耐药率较高;对美洛培南、环丙沙星的耐药菌株比率较低;多重耐药情况比较严重,部分菌株同时具有对12种抗菌药物的耐药性,具有5重耐药能力的菌株占21.74%。分离到的菌株中主要血清型为鸡白痢沙门菌(S.gallinarum),其次为鼠伤寒沙门菌(S.typhimurium)和伤寒沙门菌(S.typhi)。  相似文献   

3.
为了解上海市农贸市场和超市中鸭源沙门菌的污染情况、血清型分布及耐药情况,于2014年从上海市各大农贸市场和超市随机采集鸭肉、鸭粪便样品186份,通过常规分离鉴定和PCR鉴定,获得了44株鸭源沙门菌。对分离株采用Kauffman-White法进行血清学分型和K-B纸片法进行16种抗生素的药敏试验。结果显示,鸭源沙门菌分离率为23.65%,共鉴定出13种血清型,优势血清型为鼠伤寒(43.18%)。耐药性结果显示,分离株对萘啶酸(63.64%)的耐药率最高,对磺胺异恶唑(47.73%)、链霉素(47.73%)、四环素(40.91%)的耐药率次之,对头孢吡肟(4.54%)、头孢噻肟(4.54%)、环丙沙星(2.27%)、庆大霉素(2.27%)等耐药率低,对氧氟沙星(0.00%)和亚胺培南(0.00%)两种药物敏感。其中,多重耐药率为43.18%,最多可耐受11种抗菌药物。表明上海市鸭源沙门菌的污染率较高,血清型分布广且耐药模式多样,多重耐药菌株较严重。提示鸭源沙门菌的流行和耐药情况值得我们密切关注,同时为进一步做好食品中沙门菌的防控提供数据支持。  相似文献   

4.
对2020年在宁夏和新疆部分动物养殖场和屠宰场分离的沙门菌(Salmonella)进行了相关抗菌药物的耐药性调查，以了解沙门菌的流行特点以及耐药性情况，一方面为国家动物源细菌耐药网提供数据，另一方面为相关养殖场临床抗菌药物的合理使用提供科学依据。首先采用沙门菌选择性培养基进行菌株的分离、纯化及鉴定，采用微量肉汤稀释法检测16种抗菌药物对分离菌株的最小抑菌浓度(MICs),采用玻片凝集法对分离菌株进行血清型鉴定。结果显示：试验共分离到60株沙门菌，其血清型有16种，主要包括鼠伤寒沙门菌(S. Typhimurium)、里森沙门菌(S. Rissen)、汤普森沙门菌(S. Thompson)、肠炎沙门菌(S. Entertidis)、德尔卑沙门菌(S. Derby)、明斯特沙门菌(S. Muenster),检出率分别为26.67%(16株)、15.00%(9株)、11.67%(7株)、10.00%(6株)、8.33%(5株)、6.67%(4株),其他9种血清型的检出率为1%～5%。分离菌株对磺胺异噁唑、四环素和氨苄西林耐药程度较高，耐药率分别达75.00%、70.00%和66.67%;对奥...  相似文献   

5.
通过调查山东省不同市肉鸡屠宰生产链中沙门菌分离株血清型、耐药性及毒力相关基因,为肉鸡的健康安全生产提供数据支撑。对前期获得的233株沙门菌通过血清型快速分型试剂盒鉴定其血清型,采用最小抑菌浓度(MIC)进行13种抗菌药物的药敏试验;应用PCR技术检测位于菌株毒力岛(SPI)和毒力质粒(spv)上共15种毒力基因的携带情况。结果显示,233株沙门菌共分属25种不同的血清型,其中肠炎沙门菌、印第安纳沙门菌、汤普逊沙门菌和德尔卑沙门菌为优势血清型。233株肉鸡屠宰生产链中沙门茵对庆大霉素的耐药率最高(100%);对多西环素、氨苄西林、大观霉素、四环素、氟苯尼考和磺胺异恶唑耐药率分别为84.94%、75.73%、67.78%、56%、52%和50%。72%的菌株表现为多重耐药(≥3),产生了57种耐药谱型。沙门菌的毒力岛基因sptP、sseL、mgtC、siiE、sopB的携带率是100%,毒力基因spvA、spvB、spvC、spvD、spvR、iacP、avrA、prgK、fima和stn的检出率分别是60%、34%、49%、51%、63%、99%、38%、99%、99%和99%。结果表明,在4种优势血清型中并非均出现高耐药性,出现高耐药性的血清型主要以汤普逊沙门菌为主,汤普逊沙门菌相对于其他血清型毒力基因的携带率较高,沙门菌耐药性和毒力基因的携带间并没有明显的关联性。  相似文献   

6.
为了有效防控雏鸡白痢沙门菌病,试验自保定某蛋种鸡场疑似为鸡沙门菌病雏鸡采取肝脏病料进行细菌的分离培养、生化鉴定、鸡白痢沙门菌多价血清学鉴定及沙门菌inv A基因PCR鉴定,并对分离菌进行致病性检测及对7种抗菌药物的耐药性检测。结果表明,10株分离菌的形态特征、生化特性、血清学鉴定结果符合鸡白痢沙门菌的特征,说明从病死雏鸡组织中分离出的细菌为鸡白痢沙门菌。沙门菌inv A基因PCR扩增出373 bp目的片段,进一步证实分离菌为鸡沙门菌。用分离菌株接种雏鸡,其死亡率高达90%,表明该菌株有很高的致病性。10株分离菌株对阿莫西林和青霉素耐药率达100%,对阿齐霉素的耐药率达90%,而对阿米卡星、头孢唑林和庆大霉素耐药性较弱、对氧氟沙星较为敏感。3耐菌株多重耐药比例为42.86%,4耐菌株多重耐药比例为57.14%,5耐菌株多重耐药比例为71.43%,说明雏鸡沙门菌分离株对所试抗菌药物均表现出不同程度的耐药性,且表现出多重耐药现象。本试验为鸡白痢沙门菌病的防治提供参考。  相似文献   

7.
为了解市售鲜鸡肉中污染的与临床病鸡感染的沙门菌在血清型与耐药性的差异,对2012-2014年间自南宁市售鲜生鸡肉和同期送检病鸡中分离到的沙门菌进行了血清型分析以及耐药性的比较。结果显示,32株鸡肉源性沙门菌的血清型分布广泛,包括有德比、纽兰和阿贡纳沙门菌等16种血清型;39株病鸡源性沙门菌的血清型则较为单一,只有鸡白痢、鼠伤寒和鸡伤寒沙门菌3种血清型;两种来源的沙门菌均对四环素、复方新诺明、甲氧苄啶,氨苄西林及阿莫西林有较高的耐药性;另外,病鸡源性分离株中多重耐药菌株的比例(89.7%)比鸡肉源性的(56.2%)高。本研究表明,南宁市售鲜生鸡肉与病鸡中的沙门菌分离株在血清型来源上完全不同,提示市售鸡肉中污染的食源性沙门菌并非来自饲养阶段的患病鸡;病鸡源性沙门菌的耐药情况较鸡肉源性沙门菌的更为严重。  相似文献   

8.
本试验旨在通过监测沙门菌在生猪屠宰各环节和猪肉产品中的污染分布和耐药状况，揭示沙门菌在猪肉生产过程中的传播及耐药情况。选取山东省5个代表性生猪屠宰场为风险观测点，采集肛拭子、屠宰环境拭子、水样品和胴体拭子共552份，参照食品安全国家标准和基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)对其中沙门菌进行分离培养和鉴定；参照微量肉汤稀释法检测其药物敏感性；利用多位点序列分型(MLST)鉴定沙门菌的基因型。结果显示，共分离获得58株沙门菌，总阳性检出率为10.5%(58/552);中小型屠宰场(15.6%,33/212)较大型屠宰场(7.4%,25/340)沙门菌污染严重；屠宰环节预冷前样品检出率最高，为26.7%(8/30);不同类型样品中肛拭子样品的检出率最高，为15.1%(16/106)。耐药性检测结果显示，58株沙门菌对氨苄西林的耐药率最高(96.6%,56/58),多重耐药率为96.6%(56/58);中小型屠宰场分离株较大型屠宰场的整体耐药严重，胴体拭子(46.4%,26/56)和肛拭子(28.6%,16/56)分离株的多重耐药率高于环境拭子(25.0%,14/56)...  相似文献   

9.
为探究不同健康状态和不同饲养环境下沙门菌对临床常用抗菌药物的耐药情况及耐药基因携带情况,采用琼脂稀释法对健康与患病、家养与流浪宠物粪样中分离出的99株沙门菌进行临床常用12种抗菌药物的耐药性检测;用PCR方法对获得的耐药菌株进行相关耐药基因的检测。结果:分离出的宠物源沙门菌对所有被检的抗菌药物耐药率均未超过10.0%,对头孢噻呋、安普霉素、阿米卡星、卡那霉素和硫酸庆大霉素100%敏感;患病动物源沙门菌对被检抗菌药物的耐药率高于健康动物源沙门菌,家养动物源沙门菌对被检抗菌药物的耐药率高于流浪动物源沙门菌(P<0.05)。耐药基因检测结果显示:floR的检出率为3.0%;ant(3″)-Ia、tetA、tetB检出率均为2.0%;blaTEM、oqxA、aac(6′)-Ib-cr基因检出率均为1.0%,未检出其他被检基因。结果表明,新疆乌鲁木齐市不同健康状态和不同饲养环境下宠物源沙门菌对被检抗菌药物的耐药率及耐药基因携带率低,仅有2株沙门菌存在ant(3″)-Ia+tetA+tetB 3种耐药基因共存情况。根据试验结果发现检出的耐药表型与耐药基因之间存在相关性,可根据药敏试验结果合理选用抗菌药物进行临床细菌病的治疗。  相似文献   

10.
为了解青岛地区肉鸡生产链中各环节沙门菌的分布及其血清型状况,采用常规培养与PCR鉴定对不同环节沙门菌进行分离。采用玻片凝集试验与x MAP~#174;液相芯片分型法进行血清型鉴定。结果青岛地区肉鸡生产链沙门菌总阳性率为10.10%。养殖环节中种鸡场、孵化场、商品鸡场的分离率分别为0.67%、37.76%、9.20%;屠宰加工过程中淋洗前、淋洗后、预冷液、预冷后、分割环节分离率分别为3.69%、3.86%、5.7%、16.16%、31.43%;市售成品为17.15%。78.65%菌株为肠炎沙门菌。结论种鸡场沙门菌净化与孵化场种蛋消毒及淘汰弱雏是青岛地区肉鸡养殖环节沙门菌的关键控制措施;预冷液与分割车间的环境卫生是其屠宰加工环节的关键控制点。青岛地区肉鸡生产链中流行血清型为肠炎沙门菌。  相似文献   

11.
The objective of this study was to compare Salmonella prevalence and antimicrobial resistance between pasture and conventional poultry farms. We collected the first Salmonella prevalence and antimicrobial resistance data on pasture poultry farms. Fecal droppings were collected from 31 farms from Wisconsin (nine farms from each production type) and the Southeast (North Carolina, Virginia, and South Carolina; five conventional and 10 pasture poultry farms) in a 1-yr period. The specimens were cultured for Salmonella and tested for resistance to 12 antimicrobials. A univariate analysis was conducted to determine the significant differences in prevalence and resistance. At the farm level, no significant difference in Salmonella prevalence was found on 33% pasture and 47% conventional poultry farms (P= 0.4928). On an individual specimen level, flocks reared conventionally had higher prevalence than in pasture (P < 0.0001). Of all the isolates found to have resistance, 80% were from the Southeast. Of all the pasture isolates tested, 5% (8 of 162) were resistant to ceftriaxone, which is higher than previously reported from any production setting. None of the isolates from conventional flocks showed resistance to ceftriaxone. Multidrug resistance (resistance to three or more classes of antimicrobials) was found in 69% of the isolates from conventional farms and 11% on pasture farms in the Southeast (P < 0.0001), with the predominant resistance type of AmCSSuTeAx (ampicillin, chloramphenicol, streptomycin, sulfasoxazole, tetracycline, amoxicillin/clavulanic acid; 62%). About 5% of the pasture isolates from the Southeast showed the AmAxCFCRO (ampicillin, amoxicillin/clavulanic acid, cephalothin, ceftriaxone) multidrug resistance pattern. None of the isolates from Wisconsin were found to be multidrug resistant.  相似文献   

12.
In 2009, 1462 Escherichia coli isolates were collected in a systematic resistance monitoring approach from primary production, slaughterhouses and at retail and evaluated on the basis of epidemiological cut-off values. Besides resistance to antimicrobial classes that have been extensively used for a long time (e.g. sulphonamides and tetracyclines), resistance to (fluoro)quinolones and third-generation cephalosporins was observed. While in the poultry production chain the majority (60%) of isolates from laying hens was susceptible to all antimicrobials tested, most isolates from broilers, chicken meat and turkey meat showed resistance to at least one (85-93%) but frequently even to several antimicrobial classes (73-84%). In the cattle and pig production chain, the share of isolates showing resistance to at least one antimicrobial was lowest (16%) in dairy cows, whereas resistance to at least one antimicrobial ranged between 43% and 73% in veal calves, veal and pork. Resistance rates to ciprofloxacin and nalidixic acid in isolates from broilers were 41.1% and 43.1%, respectively. Likewise, high resistance rates to (fluoro)quinolones were observed in isolates from chicken meat and turkey meat. In contrast, ciprofloxacin resistance was less frequent in E.?coli isolates from the cattle and pig production chain with highest rate in veal calves (13.3%). Highest resistance rates to cephalosporins were observed in broilers and chicken meat, with 5.9% and 6.2% of the isolates showing resistance. In dairy cattle and veal, no isolates with cephalosporin resistance were detected, whereas 3.3% of the isolates from veal calves showed resistance to ceftazidime. Resistance to (fluoro)quinolones and cephalosporins in E.?coli isolates is of special concern because they are critically important antimicrobials in human antimicrobial therapy. The emergence of this resistance warrants increased monitoring. Together with continuous monitoring of antimicrobial usage, management strategies should be regularly assessed and adapted.  相似文献   

13.
OBJECTIVE To identify the common serotypes and antimicrobial resistance patterns of Salmonella spp. associated with diarrhoea in Australian dairy calves under the age of 6 weeks. DESIGN Cross-sectional study. METHODS Faecal samples were collected from outbreaks of diarrhoea in dairy and dairy beef calves less than 6 weeks old. Samples were screened for Salmonella using standard enrichment culture techniques. The antimicrobial susceptibility to 12 commonly used veterinary and human antimicrobials was assessed using the Kirby-Bauer disk diffusion method and the susceptibility profiles of dairy and dairy beef properties were compared using Fisher's exact test. RESULTS Salmonella ser. Dublin, S. ser. Typhimurium and S. ser. Bovismorbificans were the three most common salmonella serotypes isolated. The majority of properties had one serotype. Most of the Salmonella isolates were not resistant to any of the antimicrobials tested. No resistance was seen to amikacin and nalidixic acid, and only one isolate was resistant to ceftiofur or amoxicillin-clavulanic acid. The most common antimicrobial resistance was to streptomycin, ampicillin or combination sulfonamides. Multi-drug resistance was detected in S. ser. Anatum, S. ser. Bovismorbificans, S. ser. Muenster, S. ser. Newport and S. ser. Typhimurium. Isolates from dairy beef properties were more likely to be resistant to ampicillin, kanamycin, neomycin, sulfamethoxazole/trimethoprim and tetracycline (P < 0.05) and were more likely to exhibit multi-drug resistance. CONCLUSION The prevalence of antimicrobial resistance in Salmonella isolates from dairy calves in Australia is low compared with that reported overseas. From a human health perspective, resistance to antimicrobials used in the treatment of human salmonellosis was infrequent.  相似文献   

14.
Surveillance is an important component of an overall strategy to address antimicrobial resistant bacteria in food animals and the food chain. The poultry market has many points of entry into the Canadian food chain, and some production practices are underrepresented in terms of surveillance. For example, pathogen carriage and antimicrobial resistance surveillance data are limited in smallholder chicken flocks raised for slaughter at provincially inspected abattoirs. In Canada, antimicrobial resistance in Escherichia coli and Salmonella isolated from commercial broiler chicken flocks, slaughtered at federally inspected abattoirs, is monitored by the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS ). The objective of this study was to establish baseline information of antimicrobial resistance presence in E. coli and Salmonella isolated from smallholder flocks in Ontario, utilizing CIPARS collection and isolation methodologies, and to compare findings with CIPARS federally inspected abattoir data from Ontario, Canada. Five chickens per flock were sampled from 205 smallholder flocks. Of 1,025 samples, the E. coli prevalence was 99% (1,022/1,025), and 47% (483/1,022) of positive E. coli isolates were resistant to one or more of the 14 antimicrobials. Furthermore, as compared to results reported for the CIPARS commercial flocks, E. coli isolates from smallholder flocks had significantly lower resistance prevalence to six of 14 individual antimicrobials. Recovery of E. coli did not differ between federally inspected and provincially inspected flocks. Salmonella prevalence at the bird level in smallholder flocks was 0.3% (3/1,025), significantly lower (p  ? 0.0001, 95% CI 0.080%–0.86%) than federally inspected commercial flocks. The overall differences found between the commercial and smallholder flocks may be explained by differences in poultry husbandry practices and hatchery sources.  相似文献   

15.
The resistance patterns of Campylobacter spp. isolated from retail broiler chicken meat originating either from Estonia, Lithuania or Latvia collected in Estonia were determined. Additionally, in collaboration with the laboratories of several Estonian hospitals, antimicrobial susceptibility patterns were determined for Campylobacter isolates from patients with severe Campylobacter enteric infections. The isolates were identified at the species level by the PCR method. Respectively, 88.8% of the isolates were C. jejuni, and 11.2% were C. coli. In total, 126 Campylobacter isolates of broiler chicken meat and human origin were tested for minimal inhibitory concentrations (MICs) with the broth microdilution VetMICTH method (National Veterinary Institute; Uppsala, Sweden) for a total of six antimicrobials. Resistance to one or more antimicrobials was detected in 62 (63.3%) of Campylobacter broiler chicken meat isolates and in 20 (71.4%) of human‐origin isolates. Large proportions of the broiler chicken meat isolates were resistant to ciprofloxacin (60.2%). Multidrug resistance (i.e. to three or more unrelated antimicrobials) was detected in five (5.1%) C. jejuni isolates. Among the human isolates, 20 (71.4%) were resistant to fluoroquinolones, and two (7.1%) C. jejuni isolates exhibited multidrug resistance. The chicken meat isolates of Estonian origin were the most susceptible. However, a high proportion of fluoroquinolone‐resistant C. jejuni isolates were found in Latvian and Lithuanian products. The results of this study indicate that the problems caused by the inappropriate use of antimicrobials extend beyond the country in which a food originates; therefore, both domestic and international interventions and agreements are required to implement common policies on antimicrobial usage and to minimize the emergence of Campylobacter drug resistance.  相似文献   

16.
OBJECTIVE: To evaluate the antimicrobial susceptibility patterns of Salmonella isolates from feedlot cattle. DESIGN: Cross-sectional study. SAMPLE POPULATION: 263 Salmonella isolates. PROCEDURES: Fecal samples were collected from the floor of 2 pens in each of 100 feedlots. Two hundred eighty Salmonella isolates were recovered after bacteriologic culture from 38 pens. Of these, 263 isolates were available for antimicrobial susceptibility testing to 16 antimicrobials, using microbroth dilution breakpoint plates. RESULTS: Less than 5% of isolates were resistant to any of the antimicrobials tested, with the exception of sulfamethoxazole (15; 5.7%) and tetracycline (61; 23.2%). Most isolates (197; 74.9%) were susceptible to all antimicrobials tested, whereas 18 (6.8%) were resistant to 2 or more antimicrobials. The percentage of isolates with resistance to any antimicrobial varied by serotype. The percentage of isolates resistant to various antimicrobials was not related to concurrent use of antimicrobials in the feed. CONCLUSIONS AND CLINICAL RELEVANCE: With the exception of tetracycline and sulfamethoxazole, resistance of Salmonella isolates to any of the antimicrobials was uncommon. Most isolates were susceptible to all antimicrobials tested. Antimicrobial resistance was not related to the presence of antimicrobials in the ration being fed at the time of sample collection.  相似文献   

17.
The current study aimed at determining the prevalence and the antimicrobial resistance profiles of thermophilic Campylobacter spp. infecting broiler chickens. A total of 240 caecal samples from six slaughterhouses were examined for the presence of Campylobacter spp. C. jejuni was detected in 40.4% (97/240) of the samples and C. coli in 12.1% (29/240). The agar disc diffusion method and the E-test were used for testing the antimicrobial susceptibility of C. jejuni and C. coli isolates. C. jejuni isolates were most resistant to nalidixic acid (79.4%) followed by tetracycline (76.3%), ciprofloxacin (74.2%) and enrofloxacin (15.5%). Among the C. coli isolates, the frequency of resistance to nalidixic acid and ciprofloxacin was the same at 65.5%. The predominant profiles of multidrug resistance to three or more antimicrobials in C. jejuni and C. coli were determined as tetracycline/nalidixic acid/ciprofloxacin resistance (48.5%) and tetracycline/nalidixic acid/ciprofloxacin/enrofloxacin resistance (51.7%), respectively. To prevent the transmission of antimicrobial-resistant bacteria of animal origin to humans, it should be noted that high proportions of multidrug resistance were found in both species.  相似文献   

18.
A survey to estimate the prevalence of antimicrobial resistance in Escherichia coli was conducted in 7 Canadian federally inspected processing plants during 2001. Escherichia coli isolates were recovered during routine hazard analysis critical control point sampling from beef carcasses and trim and subsequently tested for their antimicrobial susceptibility by using susceptibility panels. Of the 2653 isolates analyzed, 68% were sensitive to all 18 antimicrobials tested. For 14 of the 18 antimicrobials evaluated, the percentage of resistant isolates was < or = 1. Twenty-five percent of the isolates were resistant to tetracycline, 9% to sulfamethoxazole, 7% to streptomycin, and 3% to ampicillin. Multiple resistance was found in 12% of the isolates, with 7% showing resistance to 2 antimicrobials, 2% to 3 antimicrobials, 2% to 4 antimicrobials, and 1% to 5 or more antimicrobials. Forty-five different antimicrobial resistance patterns were observed. The reasons for the development of the antimicrobial resistance were not investigated in this study. This study was useful as a pilot to help to develop a national antimicrobial resistance surveillance program in Canada. This study indicates that laboratory standardization is possible for consistent results across the country and that the indicator organism, E. coli, is fairly easy to obtain for surveillance but Salmonella are not, due to their low prevalence in beef.  相似文献   

19.
为了解广东省禽场和猪场沙门菌的血清型和耐药性情况,本研究2015年从广东省多个养殖场共采集样品126份,分离出沙门菌24株,分离率为19.04%。采用Kauffmann-White法、Kirby-Bauer法和PCR方法对分离株进行了血清型鉴定、药敏试验和Ⅰ类整合子检测。血清型鉴定结果显示,24株沙门菌共鉴定出4种血清型,分别是鼠伤寒沙门菌(14株)、印第安纳沙门菌(8株)、科瓦利斯沙门菌(1株)和阿尔巴尼沙门菌(1株)。药敏试验结果显示,分离株对四环素(83.33%)、氨苄西林(70.83%)、磺胺异恶唑(70.83%)、萘啶酸(66.67%)、复方新诺明(58.33%)、卡那霉素(54.17%)、阿米卡星(54.17%)、庆大霉素(50.00%)的耐药率较高,有54.17%(13/24)的菌株对8种及8种以上抗菌药物耐药。Ⅰ类整合子检测结果显示,Ⅰ类整合酶阳性率为29.17%(7/24),整合酶阳性菌株中仅有1株扩增到携带耐药基因aadA2的基因盒。上述结果表明,广东省禽场和猪场沙门菌分离率较高,耐药情况严重,菌株的耐药性与其血清型和Ⅰ类整合子的携带有一定的相关性。  相似文献   

20.
The study objective was to describe and evaluate antimicrobial resistance profiles in Salmonella isolated from Alberta swine finishing farms. Salmonella isolates (n = 322) were obtained from 192 fecal and 84 environmental samples of the 60 Salmonella-positive swine finishing farms. Isolates were classified susceptible, intermediate or resistant based on NCCLS guidelines. More than half of the isolates (53.4%) were susceptible to all of the 18 antimicrobials in the testing panel. No resistance was observed to amikacin, amoxicillin/clavulanic acid, cefoxitin, ceftiofur, ceftriaxone, cephalothin, ciprofloxacin, imipenem or nalidixic acid. Less than 1% of isolates were resistant to apramycin, gentamicin and trimethoprim/sulfamethoxazole. Higher frequencies of resistance were observed for chloramphenicol (4.7%), ampicillin (7.8%), kanamycin (11.8%), sulfamethoxazole (21.1%), streptomycin (25.5%) and tetracycline (38.8%). Eleven Salmonella serovars had isolates with resistance to > or =3 antimicrobials. The most frequently resistant serovar was Salmonella Derby, with 27 (38.0%) isolates resistant to > or =3 antimicrobials, including resistance to five and six antimicrobials. An absence of resistance to cephalosporins and fluoroquniolones and a low proportion of isolates resistant to amikacin, amoxicillin/clavulanic acid, apramycin, gentamicin and trimethoprim/sulfamethoxazole are encouraging findings from public health and animal health perspectives. Frequent resistance observed for ampicillin, kanamycin, sulfamethoxazole, streptomycin and tetracycline, antimicrobials commonly used in veterinary medicine for decades, indicates an urgent need to utilize these antimicrobials more prudently if their benefits are to be preserved.  相似文献   

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