共查询到20条相似文献，搜索用时 31 毫秒
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Shun Maekawa Yi-Ting Wang Terutoyo Yoshida Pei-Chi Wang Shih-Chu Chen 《Journal of fish diseases》2020,43(9):963-970
Streptococcus dysgalactiae subsp. dysgalactiae (GCSD) is a Gram-positive, facultative anaerobic bacterium and mostly non-β-haemolytic with Lancefield group C antigen. GCSD infection has been identified in various vertebrates. From 2002 to the present, GCSD infection of fish has been reported to cause severe economic losses in aquaculture farms around the world. Moreover, GCSD isolates from teleosts have been identified in patients with ascending upper limb cellulitis. Therefore, the economic and clinical significance of GCSD has increased in aquaculture, livestock and human health. Many studies have been presented, from the first report of isolated GCSD in fish, to the pathogenesis, characterization, immune responses and vaccine development. In this review, we present the current knowledge of GCSD in teleosts. 相似文献
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Seo-Young Kim Hyun-Soo Kim Moonjae Cho 《Journal Of Aquatic Food Product Technology》2019,28(3):264-274
The effects of enzymatic hydrolysates of Hippocampus abdominalis on skeletal muscle growth in C2C12 myoblasts and zebrafish were investigated. The hydrolysates were prepared by enzymatic extraction using digestive proteases (trypsin and pepsin) and food grade proteases (Neutrase, Protamex, Alcalase, Kojizyme, and Flavourzyme). Among them, the Alcalase hydrolysate of H. abodminalis (AHHA) exhibited the highest muscle cell proliferation compared to other hydrolysates and aqueous extract. Moreover, AHHA treatment significantly increased creatine kinase (CK) activity and glycogen levels in the cells, which are indicators of skeletal muscle mass. In addition, AHHA downregulated the myostatin–Smad pathway, acting as a negative regulator, and upregulated the IGF-1-Akt pathway, acting as a positive regulator. In the zebrafish model, moreover, the administration of AHHA significantly enhanced the endurance against water flow and slope without training performance. These results suggested that AHHA would be a new functional food material from marine animals to grow muscle cells and to enhance muscular exercise capacity. 相似文献
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ABSTRACT: Four species of Chattonella , which are well known to form red tides that are lethal to fish, were subjected to phylogenetic analysis on the basis of the ribosomal RNA genes (rDNA), 5.8S rDNA, 18S rDNA, 28S rDNA, and the flanking internal transcribed spacers 1 and 2 (ITS1 and ITS2). The 18S rDNA sequences of C. antiqua , C. marina , and C. ovata isolated from different regions in Japan were compared. They were found to be identical with each other in a sequence 1818 bp long. The sequences of the D1/D2 region in the 28S rDNA, 5.8S rDNA, and ITS region that are known to be more variable regions were also found to be identical. These homogeneities of the rRNA gene family revealed the extremely close relatedness of C. antiqua , C. marina , and C. ovata . The sequences of C. verruculosa were different from those of these three species , resulting in an 89.2% homology in the 18S rDNA sequences, 70.4% homology in the D1/D2 region in the 28S rDNA sequences, and an 81.5% homology in 5.8S rDNA sequences and the ITS regions. Chattonella verruculosa was grouped within a single cluster composed of Dictyochophyceae rather than the other species of Raphidophyceae. 相似文献
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罗氏沼虾对维生素C的需求 总被引：9，自引：0，他引：9
以每公斤饲料分别添加０、５０、１００、２００、４００、８００ｍｇ维生素Ｃ多聚磷酸酯（ＬＡＰＰ）的半纯化饲料饲养初始体重约５．５２ｇ的罗氏沼虾Ｍａｃｒｏｂｒａｃｈｉｕｍｒｏｓｅｎｂｅｒｇｉ９周。结果表明,添加ＬＡＰＰ使罗氏沼虾的相对增重率显著升高,蜕壳频率增加,蜕壳周期缩短。添加量达到５０ｍｇ／ｋｇ的,可以保证罗氏沼虾正常蜕壳。肝胰脏和血淋巴中的Ｖｃ含量随饲料中ＬＡＰＰ水平的上升而上升,ＬＡＰＰ的添加量达２００ｍｇ／ｋｇ时,肝胰脏中的Ｖｃ达到饱和;而血淋巴中的Ｖｃ含量则随饲料中ＬＡＰＰ水平的上升一直上升。若饲料中缺乏ＬＡＰＰ,则罗氏沼虾全虾的蛋白质和粗脂肪含量下降,而肝胰脏中的粗脂肪含量上升。以增重率为指标,用突破点直线回归法确定罗氏沼虾饲料中Ｖｃ多聚磷酸酯的适宜含量为１１１．９ｍｇ／ｋｇ。 相似文献
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Vitamin C deficiency in the catfish Clarias gariepinus 总被引：3，自引：0，他引：3
Regardless of the absence of spinal deformities, vitamin C deprivation in adult-sized Clarias gariepinus challenged the physical integrity and survival of fish, inducing changes that greatly resembled those of fingerlings of other species. Vitamin C was presumed to be a dietary facilitator, which when deprived in the diet of C. gariepinus , would obstruct intestinal absorption of iron and/or impair its release from the reticulo-endothelial stores. This impaired erythrocyte synthesis led into anaemia (as indicated by lowered levels of red blood cells, haemoglobin and haematocrit). Leukopenia noted in fish on a scorbutic (lacking vitamin C) diet suggested functional depression of phagocytic engulfment with increased susceptibility to the deteriorating action of pathogenic agents. Changes in histology included hypertrophy, oedematous separation and telangiectasia of gill lamellae. Shrunken glomeruli, sloughed Bowman's capsules and tubular dilation and distortion were also shown. Shrunken hepatocytes and the occurrence of yellowish ceroid pigments were the concrete histopathological signs in liver. Vitamin C is thus suggested as a potent antioxidant that offers protection against oxidative damage to various fish tissues. 相似文献
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Jozef H. Blom Konrad Dabrowski James Ebeling 《Journal of the World Aquaculture Society》2000,31(1):115-118
Abstract.— Despite a large economical value of the ornamental fish trade, little information is available on the dietary ascorbic acid requirements of these species. Ascorbic acid requirements of fishes of the cichlid family appear to vary widely. Juvenile angelfish, a widely produced ornamental cichlid, were maintained on diets containing graded levels of ascorbyl monophosphate. Liver ascorbic acid concentrations after 96 d of feeding were significantly reduced in groups receiving 120 mg or less ascorbic acid equivalents/kg diet. However, no differences in growth or mortality between groups were found, and no external signs of ascorbic acid deficiency were observed, indicating a high resistance of this species against prolonged ascorbic acid deficiency. Based on the long possible life span of angelfish in the aquarium, we proposed a conservative dietary ascorbic acid requirement of 360 mg/ kg diet, necessary to maintain maximum tissue storage of this vitamin. 相似文献
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为了研究鱼类半胱氨酸蛋白酶抑制剂(cystatin)的功能并探索其在水产品加工和病害防治中的应用潜力，将改造后的中华鲟(Acipenser sinensis)cystatin C cDNA亚克隆到原核表达载体pBV220，构建表达cystatinc的大肠杆菌基因工程菌。该工程菌经温度诱导、SDS—PAGE检测，在约12．4kD处有一特异蛋白带，该特异蛋白的含量约为菌体总蛋白的25％。重组半胱氨酸蛋白酶抑制剂经洗涤、溶解、透析、复性后纯度为85％，实现了中华鲟cystatinC在大肠杆菌中的高效表达。木瓜蛋白酶活性抑制实验结果表明该重组cystatinc具有明显的酶活抑制作用。 相似文献
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利用逆转录聚合酶链式反应(RT-PCR)和cDNA末端快速扩增(RACE)等方法,克隆获得剑尾鱼卵黄蛋白原C(Vg C)基因的全长cDNA序列。剑尾鱼Vg C基因cDNA序列全长4 011 bp,其5′非编码区包含12 bp和3′非编码区包含246 bp;含有一个3 753 bp的开放阅读框(ORF),编码1 250个氨基酸,推测其编码氨基酸分子量大小为141.7 ku,编码氨基酸序列与其他鱼类卵黄白原C编码氨基酸序列相似性在44%～85%。荧光定量PCR结果显示,Vg C在剑尾鱼肝脏中表达量最高,脾、肾、卵巢中有微量表达,脑、肌肉、鳃中几乎没有检测到表达;对不同时间暴露在雌激素中剑尾鱼肝脏进行实时荧光定量PCR表达分析的结果表明,Vg C在剑尾鱼肝脏中第5天表达量最高,随后降低,第9天后维持相对较低的表达量。研究首次克隆了剑尾鱼Vg C基因全长cDNA序列,并对Vg C在剑尾鱼体内表达组织器官分布及雌激素诱导后不同时间表达谱进行了初步研究,为剑尾鱼生殖生理及环境污染物监测应用等不同领域研究打下重要基础。 相似文献
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采用半定量RT-PCR方法研究了在体外培养的猪成纤维细胞内,添加0.5,5,10,30μg/μL浓度的维生素C后,分别作用12、24、48和72h后,视黄醇结合蛋白基因(RBP4)的表达变化情况。结果表明:维生素C可显著抑制RBP4基因的表达。 相似文献
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Dietary vitamin C requirement of red drum Sciaenops ocellatus 总被引：5，自引：0，他引：5
A feeding trial was conducted to determine the minimum dietary vitamin C requirement of juvenile red drum and characterize signs of vitamin C deficiency. Semipurified diets containing 400 g crude protein kg–1 from lyophilized red drum muscle and crystalline amino acids were used in the feeding trial. The basal diet without supplemental vitamin C contained ≈ 6 mg vitamin C kg–1 and was fed for a 1-week conditioning period. Red drum, initially averaging ≈ 3.6 g each, were fed diets supplemented with 0, 10, 20, 30, 45, 60, 75, or 150 mg vitamin C kg–1 as ascorbate polyphosphate for a period of 10 weeks. Fish fed the basal diet began to exhibit overt signs of vitamin C deficiency, including reduced weight gain, lordosis, scoliosis and loss of equilibrium after 8 weeks. Total ascorbate was undetectable in liver of fish fed the basal diet while liver ascorbate levels in fish fed the other diets generally reflected dietary supplementation. Regression analysis of weight gain data using the broken-line model resulted in a minimum vitamin C requirement ( ± SE) of 15 ± 3 mg kg–1 diet. 相似文献
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为研究草鱼补体蛋白5a (complement component 5a,C5a)、补体蛋白5a受体(C5a receptor,C5aR)和凝血因子Ⅱ(blood coagulation factorⅡ,FⅡ)在感染草鱼呼肠孤病毒(grass carp reovirus,GCRV)时的蛋白表达和相互作用,针对草鱼C5a和FⅡ蛋白构建了原核表达体系、针对草鱼C5aR蛋白构建C5aR-KLH偶联物,纯化蛋白,免疫日本大耳白兔制备3种蛋白的多克隆抗体。用蛋白质印迹(Western blot)、免疫共沉淀(Co-IP)和拉下(pull down)实验检测3种蛋白表达与互作关系,Western blot结果显示,C5a和C5aR在健康草鱼的肝脏、脾脏、肾脏、头肾、肠、鳃和肌肉中均有蛋白表达,FⅡ在肝脏、脾脏和肠中表达,而在肾脏、头肾、鳃和肌肉中不表达;在感染GCRV的草鱼肝脏组织中C5a、C5aR和FⅡ蛋白均随病程进展呈现上升趋势。Co-IP结果显示,在GCRV处理后,C5a、C5aR和FⅡ蛋白具有相互作用关系。pull down结果显示,C5a pull down共鉴定得到C3、RIG-I等2... 相似文献
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Malcolm Jobling 《Aquaculture International》2011,19(6):1231-1232
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Influence of vitamin C on the immune system of salmonids 总被引：3，自引：0，他引：3
Abstract. Trials were run to determine the immunomodulatory effect of dietary vitamin C in rainbow trout. Oncorhynchus mykiss (Walbaum), and Atlanticsalmon, Salmo Salar L. We first investigated whether depletion of ascorbic acid (AA) could depress the immune system. Subsequently, we focused our studies on the stimulatory effect of a high dietary dose of stable vitamin C derivatives (1000mg AA-equivalent per kg of feed) in order to determine their prophylactic role. Three trials were run with rainbow trout fed, for periods of time varying from 2 to 6 months, a high dietary dose of ascorbate-2-polyphosphate (AAPP) or L-ascorbate-2-monophosphate calcium salt (AAMP) equivalent to 1000mg AA per kg of feed. The high dietary level of vitamin C was compared to 60mg AA-equivalent per kg of feed. Another experiment was conducted with Atlantic salmon pre-fed AAPP at 60mg/kg for 6 months followed by AAPP at 1000mg/kg for 2 months. Various immune parameters were tested such as mitogen-induced proliferation, serum complement level, phagocytosis and natural cytotoxicity. Leucocyte and plasma AA contents were also determined. The immune response in vitamin-C deficient trout was not strongly different from that in trout fed the minimal growth requirement level. Serum complement level was not affected by vitamin C. A high dietary dose of vitamin C was found to increase trout and salmon leucocyte AA content, mitogen-induced proliferation of lymphocytes, and chemiluminescence response of leucocytes with a higher constancy, when the cells were stimulated with opsonized zymosan and with luminol as a substrate. Natural cytotoxicity was enhanced in trout fed a high dietary dose of vitamin C. The results of these immunity studies support supplementation levels of vitamin C which are much higher than the minimal requirement known to prevent deficiency symptoms and promote normal growth. Further investigations on the time-dose response are needed to define quantitatively the high dietary dose of vitamin C. 相似文献
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Calcium uptake in experimentally induced scorbutic snake heads, Channa (= Ophicephalus) punctatus, fed vitamin C-deficient diet for 210 days is reported, using 45Ca as tracer. When compared with a parallel control on completely synthetic diet, the results show decreased absorption and utilization of calcium by gill, skin, muscle and bone of scorbutic fish from surrounding water. The physiological significance of these results is discussed, and its practical utility in aquaculture is indicated. 相似文献