共查询到19条相似文献，搜索用时 218 毫秒
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为探究不同饲养模式下滩羊血清代谢通路及差异代谢物的变化，本试验选取24头9月龄体重为（30.12±3.23）kg、体况相似的去势滩羊，随机分为舍饲组和放牧组，3个月后颈静脉血采血进行非靶向代谢组学分析。结果显示：放牧组和舍饲组中共筛选出234个差异代谢物，其中143个差异代谢物上调，91个差异代谢物下调。通过富集分析得到49个代谢通路，根据富集程度及其与脂质相关性筛选出15个代谢通路，其主要参与机体核苷酸代谢、脂代谢、硒代谢和维生素代谢。综上，放牧饲养模式主要对滩羊的核苷酸代谢、脂代谢和维生素代谢产生影响从而对肉质起到调控作用，而舍饲饲养模式通过硒代谢来调控滩羊肉质。 相似文献
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本研究旨在运用气相色谱-质谱联用(GC-MS)技术分析急性热应激肉鸡血清物质代谢组学变化,鉴定出差异代谢物和差异代谢通路,从代谢组学层次揭示肉鸡急性热应激的营养物质代谢途径变化的发生机制。试验选择20只35日龄爱拔益加(AA)肉鸡,随机均分为2组,每组5个重复,每个重复6只鸡。对照组按常规饲养标准温度饲养,热应激组于(32±1)℃下饲养,均持续12 h。翅下静脉采血2 m L,分离血清,经衍生化处理,运用GC-MS技术获得2组肉鸡血清代谢图谱,对试验收集的数据进行代谢物主成分分析和偏最小二乘法分析,鉴定差异代谢物并进行差异代谢通路富集分析。结果表明:利用GC-MS技术,在肉鸡血清中共检测到144种代谢物,筛选出30种差异代谢物[变量权重值(VIP)1,P0.05]。其中延胡索酸、羟丁酸、丙氨酸等14种代谢物含量上调[差异倍数(FC)1],草酸、苹果酸、二羟基丙酮等16种代谢物含量下调(FC1)。代谢通路富集分析表明机体三羧酸循环、半乳糖代谢、丙酸代谢、脂肪酸生物合成等代谢通路发生显著改变(P0.05或P0.01)。由此可见,肉鸡发生急性热应激时血清代谢物和物质代谢途径发生显著改变。鉴定的30种差异代谢物和7种差异代谢通路可作为标志物用于揭示急性热应激的发病机理。 相似文献
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试验旨在利用色谱-质谱联用（liquid chromatograph-mass spectrometer,LC-MS）代谢组学技术分析植物乳杆菌发酵黄芪的代谢产物,并探索其互作发酵机制。分别采取植物乳杆菌发酵黄芪（FT组）和未发酵黄芪固态粉末（CT组）,经样本前处理、LC-MS分析、生物学信息分析等探寻差异代谢物并分析代谢通路。结果显示,总离子流图峰图重现性良好,发酵黄芪主要代谢物共鉴定出183种代谢成分,正离子和负离子模式样品间关系PCA图均能良好区分。火山图分析表明,FT和CT组间的代谢物变化具有差异性,正离子模式上调的1 416个代谢物富集到83个代谢途径;下调的935个代谢物富集到83个代谢途径;负离子模式上调的1 040个代谢物富集到52个代谢途径,下调的809个代谢物富集到45个代谢途径。发酵黄芪差异代谢产物酸类、脂类、酮类等氨基酸显著增加,烯类等氨基酸显著下调,其中上调代谢物15个,下调代谢物2个,关键代谢产物主要为α-硫酸二乙酯、2-甲基柠檬酸、3-异丙烯基-6-氧代庚酸等,涉及到丙酮酸代谢、丙酸代谢、半乳糖代谢等途径。本研究结果为发酵黄芪的代谢产物、发酵互作机制和临床应用提供理论依据。 相似文献
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驴肌内脂肪沉积关键调控因子研究 总被引：2，自引：2，他引：0
旨在基于转录组（RNA-Seq）和代谢组（UPLC-MS/MS）关联分析，筛选驴肌内脂肪沉积的关键调控因子。本研究选用饲养条件相同、平均体重为236.10 kg的雌性广灵驴30头，对其背最长肌进行肌内脂肪（IMF）含量的测定，选择年龄一致的驴，并根据其IMF含量的高低分为两组：低肌内脂肪组（L组，每组3头）与高肌内脂肪组（H组，每组3头），进行转录组学和代谢组学测序分析，对差异表达基因（DEGs）和差异代谢物（DAMs）进行KEGG关联分析和相关性分析，并构建相关性网络图。结果表明，在L组和H组之间共鉴定出72种差异代谢物，其中27种代谢物上调，45种代谢物下调；关联分析表明，在差异表达基因和差异代谢物之间共有35条共富集通路，其中甘油脂代谢、甘油磷脂代谢、花生四烯酸代谢、亚油酸代谢和不饱和脂肪酸的生物合成5条通路与IMF沉积相关，有8个差异表达基因和15种差异代谢物在这5条通路中富集，包括DGKA、DGAT2、PLA2G3、GPCPD1和SCD等差异表达基因以及甘油-3-磷酸（glycerol-3-phosphate，G3P）、溶血卵磷脂酸16：0（lysophosphatidic acid，LPA）、溶血卵磷脂酰胆碱16：0（lysophosphatidylcholine，LPC）、二十二碳六烯酸（docosahexaenoic acid，DHA）和花生四烯酸（arachidonic acid，AA）等差异代谢物。另外，通过网络图分析发现，甘油脂代谢通路和甘油磷脂代谢通路中的DGAT2、PLA2G3、GPCPD1和DGKA基因与它们相应的代谢产物具有高度相关性。本研究所鉴定差异表达基因可作为IMF沉积相关的潜在候选基因，为今后进一步探究驴IMF沉积的分子调控机制和驴的肉品质分子育种提供理论依据。 相似文献
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试验旨在比较不同羽色的毛囊代谢组学差异,以探究鸡不同羽色形成的代谢机制。试验对红羽、浅红羽和白羽三组每组各7只鸡,采集其毛囊组织,使用液相色谱-质谱（Liquid chromatography mass spectrometrhy,LC-MS）联用技术对毛囊组织进行代谢物检测,鉴定3组之间的差异代谢物,并对其代谢通路进行分析。结果显示：在红羽、浅红羽和白羽组中共检测到代谢物4 426个,筛选到红羽组和白羽组间的显著差异代谢物共464个,其中在红羽组显著上调的195个,下调的269个,对这些代谢物进行富集分析,筛选出8个代谢物作为导致羽色变化的标志物,其中cAMP、甲氧沙林、GDP和半胱氨酰多巴在红羽组上调,（S）-羟基己酰辅酶A、4-吡哆酸、抗坏血酸和丁酰辅酶A在红羽组下调。研究表明cAMP、甲氧沙林、GDP等8种差异代谢物和其涉及的通路可能是造成红羽、浅红羽和白羽这3种不同羽色的原因,试验可为鸡红羽表型的形成机制解析和育种应用提供参考。 相似文献
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《畜牧兽医学报》2018,(12)
旨在应用代谢组学技术筛选产后卵巢静止奶牛乳清和血清的差异代谢物,明确其与奶牛卵巢静止的关系。本研究选取产后45～60 d的正常发情奶牛和卵巢静止奶牛各14头进行试验。应用一维氢谱核磁共振(~1H-NMR)技术对两组奶牛的乳清和血清进行检测,结合多元统计分析和单变量分析,筛选出组间差异代谢物,并进行生物信息学分析,阐明差异代谢物参与的代谢通路与奶牛卵巢静止的关系。结果表明,在乳清和血清中分别筛选出13种差异代谢物。与发情奶牛相比,卵巢静止奶牛乳清中6种差异代谢物含量升高:琥珀酸、磷酸肌酸、甘氨酸、肌醇、乙醇酸、乳清酸,7种下降:丙氨酸、肌酐、磷酸胆碱、乳糖、牛磺酸、半乳糖、葡萄糖-1-磷酸;血清中4种差异代谢物升高:β-羟丁酸、乙酸、谷氨酰胺、甘氨酸,9种降低:丙氨酸、琥珀酸、柠檬酸、肌酐、磷酸胆碱、葡萄糖、肌醇、酪氨酸、组氨酸。两组奶牛乳清差异代谢物主要参与牛磺酸和亚牛磺酸代谢、半乳糖代谢、原代胆汁酸生物合成等。血清差异代谢物主要参与丙氨酸、天冬氨酸和谷氨酸代谢、乙醛酸和二羧酸代谢、柠檬酸循环、苯丙氨酸、酪氨酸和色氨酸生物合成等。本研究获得了卵巢静止奶牛乳清和血清的代谢谱及其差异代谢物,结果表明了主要的糖代谢、脂代谢和氨基酸代谢的异常途径在奶牛产后卵巢静止发生中所起的作用,为今后深入研究奶牛产后卵巢静止发生机制和防治策略提供了新的方向。 相似文献
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为研究不同日龄骡鸭肝脏生长代谢物的变化差异情况,试验通过代谢组学分析70和90日龄公骡鸭肝脏差异代谢物。结果显示：70和90日龄骡鸭肝脏显著差异代谢物共44种,除包括氨基酸类（9种）、脂肪酸类（7种）、有机酸类（4种）、碳水化合物（9种）、维生素（4种）和核苷酸类（6种）等物质外,还有尿囊素、肾上腺素和D-鞘氨醇等显著差异代谢物。与70日龄骡鸭相比,90日龄骡鸭的肝脏差异代谢物表达上调的有6种,表达下调的差异代谢物有38种。代谢通路分析表明嘌呤代谢、癌症中的中枢碳代谢、环腺苷酸信号途径、脂肪细胞脂解的调控、蛋白质的消化与吸收等代谢通路在70和90日龄骡鸭的肝脏代谢中起主要调节作用。研究表明,这些代谢物的变化差异和参与的代谢通路大部分对骡鸭的肝脏生长均起到一定的保护作用,维持肝脏的生长。 相似文献
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以紫花苜蓿为研究对象,采用非靶向代谢组学方法,利用液相色谱电离串联质谱(LC-ESI-MS)分析不同生育时期苜蓿叶片中代谢物的变化。采用XCMS程序处理原始数据,然后应用软件SIMCA-P 14.1进行模式识别。通过正交偏最小二乘法判别分析(OPLS-DA)方法与单变量统计分析,以OPLS-DA模型VIP1且T检验P值0.05为筛选标准,筛选显著性差异代谢物。结果发现共27个代谢物发生显著变化,11种表达下调,16种表达上调。其中,L-谷氨酸、嘌呤、嘧啶等与粗蛋白质含量相关的代谢产物在现蕾期到中花期主要表现为下调,L-苯丙氨酸及糖类等与木质素和半纤维素合成相关的代谢产物主要表现为上调,共同导致粗蛋白质含量降低,中性洗涤纤维、酸性洗涤纤维含量增加,苜蓿的营养品质及相对饲喂价值下降。此外,乳酸及一些可溶性碳水化合物含量在中花期相对于现蕾期表现为上调,因此,中花期收获的苜蓿更宜青贮,而现蕾期收获的苜蓿粗蛋白质含量较高,更宜调制干草。 相似文献
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WEI Renyue WANG Zheng ZHOU Zhixin DENG Chaoyang HOU Kaiwen ZHENG Jiasan 《畜牧兽医学报》1956,51(9):2293-2301
The purpose of this study was to investigate the variation of differential metabolites in mammary carcinomas bearing cats and healthy cats, and to explore the relationship between differential metabolites and the occurrence of feline mammary carcinomas. In this study, 6 feline mammary carcinomas serum samples were selected as test (T) group. Meanwhile, 6 healthy cats with similar age and same breed were selected as control (C) group. Serum samples were detected by ultra-high performance liquid tandem chromatography quadrupole time of flight mass spectrometry (LC-MS). Differential metabolites were screened by principal component analysis (PCA), orthogonal projections to latent structures-discriminant analysis (OPLS-DA) and Student's t-test. Then the hierarchical clustering analysis (HCA) was carried out for the screened differential metabolites. The KEGG annotation and the metabolic pathway of differential metabolites were analyzed. The results showed that a total of 159 differential metabolites were identified in the 2 groups. Compared with C group, 49 differential metabolites were down-regulated and 110 differential metabolites were up-regulated in T group. Finally, a total of 5 differential metabolites which closely related to feline mammary carcinomas were selected. Ergothioneine (EGT) and creatine in T group were down-regulated, while indolelactic acid (IAA), choline and uric acid were up-regulated compared to C group. These differential metabolites indicated that during the development of feline mammary carcinomas, the body changes involved multiple metabolic pathways, such as glycine, serine and threonine metabolism, arginine and proline metabolism, histidine metabolism, tryptophan metabolism, purine metabolism and glycerophospholipid metabolism. This study provides a new idea for further research of the pathogenesis of feline mammary carcinomas. 相似文献
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Murakami Y Tateyama S Rungsipipat A Uchida K Yamaguchi R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(7):743-750
The involvement of cyclin A, cyclin D1 and p53 proteins in canine and feline tumorigenesis was analyzed immunohistochemically. In the present study, a total of 176 cases were examined, among which there were 108 canine cases (75 mammary lesions, 16 squamous cell carcinomas and 17 basal cell tumors) and 68 feline cases (43 mammary lesions, 20 squamous cell carcinomas and 5 basal cell tumors). Speckled nuclear staining for cyclin A was observed in 19/38 (50%) canine malignant mammary tumors and 18/37 (48.6%) feline mammary carcinomas, while this was not seen in benign mammary tumors of either dogs or cats. Marked intense nuclear cyclin A staining was seen in 7/16 (43.8%) canine squamous cell carcinomas and 18/20 (90.0%) feline squamous cell carcinomas. Only 3/17 (17.6%) canine basal cell tumors showed slight and scattered staining for cyclin A. Expression of cyclin D1 was very rare in both canine and feline tumors. Nuclear staining of p53 was found in 7/37 (18.9%) feline mammary carcinomas. Intense immunoreactivity for p53 was found in 6/16 (37.5%) canine squamous cell carcinomas and 8/20 (40%) feline squamous cell carcinomas. These results suggest that cyclin A may have a role in the proliferation of canine malignant mammary tumors, feline mammary carcinomas and squamous cell carcinomas of dogs and cats, and p53 may associate with the tumorigenesis of feline mammary carcinomas and squamous cell carcinomas of dogs and cats. 相似文献
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基于非靶向代谢组学技术,分析腹腔注射苦参碱前后昆明小鼠粪便和血浆代谢物的差异,并通过与此前16S rDNA测序结果联合分析探究苦参碱发挥药理作用的可能机理。将20只昆明小鼠随机分为2组,分别是苦参碱处理组(MT)和生理盐水处理组(NC)。苦参碱处理组每天腹腔注射40 mg·kg-1的苦参碱,连续给药5 d,每天给药2次。生理盐水处理组按照同样方式和体积腹腔注射生理盐水。给药第6天,分别收集各组小鼠的粪便和血浆,进行非靶向代谢组学检测,并与苦参碱处理组肠道菌群的16S rDNA测序结果进行联合分析。苦参碱处理组的粪便及血浆中均存在显著差异代谢物,粪便中共鉴定出97种,血浆中有44种。聚类分析显示,粪便中苦参碱组有35种代谢物上调,104种代谢物下调;血浆中苦参碱组有20种代谢物上调,35种代谢物下调。KEGG通路分析显示粪便及血浆差异代谢物被映射到Protein digestion and absorption等代谢途径。与16S rDNA测序分析得到的显著差异菌种嗜酸乳杆菌关联分析,结果表明粪便及血浆代谢物与嗜酸乳杆菌存在相关性。苦参碱可调节昆明小鼠的体内代谢,在粪便和血浆中均存在显著差异代谢物。这些差异代谢物及与菌群之间的互作可能是其发挥药理作用的关键。 相似文献
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奶牛分娩前后瘤胃代谢物变化规律及其代谢通路研究 总被引：1，自引：1，他引：0
本试验旨在阐明奶牛分娩前后瘤胃代谢物及其代谢通路的变化规律。选取10头体况和胎次相近的健康待产荷斯坦奶牛,分别于分娩前后晨饲前采集瘤胃液,采用UPLC-MS/MS代谢组学技术和MetaboAnanlyst 5.0中的通路分析方法研究奶牛分娩前后瘤胃发酵产物及其代谢通路的变化规律。结果表明,奶牛分娩后瘤胃中57种代谢物含量较分娩前发生了明显变化,其中34种代谢物含量显著降低,23种代谢物含量显著升高,9个代谢通路（牛磺酸和亚牛磺酸代谢、精氨酸和脯氨酸代谢、精氨酸的生物合成、D-谷氨酰胺和D-谷氨酸代谢、淀粉和蔗糖代谢、半乳糖代谢、丙氨酸、天冬氨酸和谷氨酸代谢、嘌呤代谢和维生素B6代谢）发生了显著变化。本研究表明,奶牛在分娩前7~10 d与氨基酸、糖、核苷酸和维生素相关的瘤胃微生物菌群组成发生了应答性改变。 相似文献
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Biphasic epithelial myoepithelial (complex) carcinomas of the feline mammary gland are rare. This article describes the pathological and immunohistochemical features and clinical outcome of eight cases of feline mammary carcinomas displaying complex morphology. This tumour type is a low grade malignancy that shows histopathological features distinctive from more common feline mammary carcinomas and from complex mammary carcinomas of dogs. It appears to have a better overall survival than other carcinomas of the mammary gland of cats. 相似文献
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Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. 总被引：2，自引：0，他引：2
D Ivanyi J M Minke C Hageman E Groeneveld G van Doornewaard 《American journal of veterinary research》1992,53(3):304-314
Expression of keratins (cytokeratins, CK) in healthy feline epithelia and 2 established feline mammary carcinoma cell lines was examined immunohistochemically and by use of immunoblotting analysis. A panel of specific anti-CK monoclonal antibodies (MAb) identifying epitopes unique to individual keratins or shared by 2 (or 3) CK polypeptides was used. Besides already available anti-human CK MAb, this panel of MAb consisted of 9 newly generated anti-human CK MAb and 1 newly generated anti-feline CK MAb. Immunohistochemical analysis on normal epithelia revealed that most of the anti-human CK MAb and the anti-feline CK MAb reacted with both feline and human epithelia, with a comparable tissue distribution pattern. However, slight differences in CK tissue distribution pattern between human beings and cats were detected by one MAb. Immunoblotting analysis revealed that all anti-human CK MAb that were immunohistochemically reactive with feline tissues detected analogous CK in cats, indicating the presence of a number of common epitopes on human and feline CK. Two continuous cell lines derived from 2 distinct feline mammary adenocarcinomas, K248C and K266, were analyzed with respect to their CK phenotype. Although no difference in CK expression between the 2 cell lines was detected in vitro, a difference in CK phenotype was detected on subcutaneous transplantation of the 2 cell lines into nude mice. Although the K248C-induced adenocarcinomas maintained the same CK phenotype as observed in vitro, the CK pattern of the K266 heterotransplants, growing as adenosquamous carcinomas, changed with squamous differentiation. Our findings confirm the high degree of homology between mammalian CK, and on the basis of those findings, we suggest that CK proteins provide a set of markers valuable for the characterization of normal and neoplastic feline tissues and for studies of squamous metaplasia. 相似文献
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Pamela M. Lee Michele C. L. Faus Michael H. Court 《Journal of veterinary pharmacology and therapeutics》2019,42(1):16-25
Clopidogrel response variability has been identified in cats. In humans, evidence suggests that variable clopidogrel active metabolite (CAM) generation is the primary explanation for clopidogrel response variability with differences in body weight, sex, and variable metabolism of clopidogrel primarily due to polymorphisms of the gene encoding cytochrome P450 (CYP) 2C19 as some proposed mechanisms. The aim of this study was to evaluate whether variation in CAM concentrations exists in healthy cats and what the cause of such variation might be. Nineteen healthy cats were given 18.75 mg clopidogrel by mouth. Blood was collected 2 hr later. Plasma CAM concentrations were measured using high performance liquid chromatography and tandem mass spectrometry. Clopidogrel metabolism was estimated by calculating CAM metabolic ratio. DNA was collected, and feline CYP2C genotyping was performed. The cats demonstrated high interindividual variation of plasma CAM concentrations. Approximately 69% of this interindividual variation was primarily explained by differences in clopidogrel metabolism as measured by CAM metabolic ratio with some influence by sex but not by weight. A single nucleotide polymorphism was identified in the feline CYP2C gene that explained in part individual differences in CAM metabolic ratio and CAM plasma concentrations. 相似文献