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《Medicinal Plant》2014,(1)
[Objective]To explore the methods for extraction and content determination of polysaccharides from Hedysayum polybotrys Hand.-Mazz.,and to observe its anti-inflammatory effect. [Methods] Polysaccharides were extracted by hot water. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chromomeric agent. [Results]The content of HPS was 4. 94% in same batch of radix hedysari slices,while that reached 45. 30% in refined radix hedysari samples. HPS inhibited the xylene-induced ear swelling in mice.[Conclusions]The phenol-sulfuric acid method is simple,rapid,and accurate,and HPS has anti-inflammatory effect. 相似文献
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[Objectives]This study aimed to establish a method for determination of adulteration dye auramine O in Gegen Qinlian tablets by HPLC-MS/MS.[Methods]The instrume... 相似文献
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GC-MS法检测广藿香挥发油的化学成分 总被引：1，自引：0，他引：1
摘要 [目的] 建立广藿香挥发油的指纹图谱检测方法,对广藿香的品质进行控制。[方法] 采用GC-MS法对广藿香挥发油成分进行定性和量化分析,并对市售的不同批次样品进行检测。[结果] 以9个共有峰为评价指标,实验的重复性、稳定性、重现性良好;不同批次的样品各组分的含量有显著差异。[结论] 以9个共有峰作为广藿香挥发油的指纹图谱,以广藿香酮与广藿香醇的峰面积比值作为控制质量的指标参数,建立了相应的指纹图谱。建议在使用广藿香时应根据产品的不同特点建立相应的指纹图谱质量标准。 相似文献
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《Medicinal Plant》2015,(Z5)
[Objective] To establish a rapid,fast and accurate high performance liquid chromatographic analysis for HERBA TAXILLI.[Methods]Quercitrin and quercetin were extracted from HERBA TAXILLI by acid hydrolysis and ultrasonic extraction. Quercitrin and quercetin contents were detected by RP-HPLC. [Results] Quercetin was the main component extracted by acid hydrolysis. There were few free quercetins by ultrasonic extraction; and they were mainly existed in the form of quercitrin. [Conclusions] Quercitrin extracted by ultrasonic extraction was used as the index for quantitative determination; detection was carried out by RP-HPLC. This method could rapidly and accurately reflect the quality of HERBA TAXILLI. 相似文献
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Hongyan WEI Jian ZHOU Jinguang LU Jingzheng SONG Qiongxi YU Zhihong JIANG 《Medicinal Plant》2019,(6):27-29
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol... 相似文献
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Chunli TANG Fengxian ZHAO Hongxia CHEN Jiabao MA Jiangcun WEI Meiyan QIU Zhen XIE 《Medicinal Plant》2019,(6):60-65
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination... 相似文献
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[Objectives] To establish a method for the determination of tannin in Sanguisorba officinalis L.,and provide guarantee for further control over quality of semi-... 相似文献
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植物根际土壤细菌的分离与鉴定 总被引：1，自引：1，他引：0
为了揭示不同植物根际土壤细菌的群落结构和多样性,以期为更好地利用植物根际土壤细菌奠定基础。笔者采用稀释分离法,对12种不同植物的根际土壤细菌进行了分离,并采用16SrDNA序列测定与系统发育分析法初步确定了细菌的分类地位。结果表明,不同种类植物的根际土壤中,可培养细菌的多样性不同,重茬地和多年生植物的根际土壤中细菌的种类和数量较多;12种植物的根际土壤中共分离得到芽孢杆菌属(Bacillus)、假单胞菌属(Pseudomonas)、短杆菌属(Brevibacterium)、节杆菌属(Arthrobacter)、沙雷氏菌属(Serratia)、类芽孢杆菌属(Paenibacillus)、不动杆菌属(Acinetobacter)和Massilia等8个属的137株细菌,其中,芽孢杆菌属为优势属,占81.0%。芽孢杆菌属内占主要比例的有：B.megaterium、B.aryabhattai、B.subtilis、B.amyloliquefaciens等种,其中B.subtilis,B.amyloliquefaciens等细菌种类据文献报道为有益微生物,可以在今后的研究中进行开发和利用。植物根际土壤中的可培养细菌种类丰富多样,有望成为有益微生物资源库进行开发利用。 相似文献
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离子注入线辣椒F2代表型观察及变异分析 总被引：1，自引：0，他引：1
【研究目的】观察离子注入线辣椒种子在F1代诱变产生的生物学效应在F2代的遗传特征。【方法】选取经离子注入后发生生物学诱变的5组F1代线辣椒种子继续在田间播种实验,观察F2代主要表型变化,并通过过氧化物同工酶的测定对离子注入引起生物化学变化进行分析。【结果】种子双面分别注入9×1011 P2+/cm2和1×1012 Cu2+/cm2组（No.21）单株产量在F2代继续保持明显优势,而其他4组F1代的一些生物学优良性状未能在F2代中继续遗传显现。【结论】F1代No.21处理组种子诱变的优良生物学性状在F2代继续得到了遗传,具有进一步选育的价值。 相似文献
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《Medicinal Plant》2014,(8)
[Objective] To determine the conten of mineral elements and analyze the characteristic elements in Gynostemma and evaluate its quality. [Methods] The content of 18 elements in Gynostemma samples of different producing areas were determined by the method of ICP-OES. The principal component analysis of SPSS was applied for the evaluation of characteristic elements in Gynostemma. [Results] The 18 mineral elements had good linear relationship. By principal components analysis,it concluded that four major factors could be used to evaluate the quality of Gynostemma. 12 mineral elements were the characteristic elements of Gynostemma. And according to the clustering results,the content of mineral elements in samples had correlation with their producing areas. [Conclusions]The method was accurate,reproducible and of high sensitivity,which could be used as the method for quantitative determination of mineral elements in Gynostemma. The clustering results indicated that 10 samples were clustered into 2 classes. 1,2,4,5,3,7 and 10 were clustered into a class and 6,9 and 8 were clustered into another class. 相似文献
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【研究目的】对甘草离体培养物中总黄酮提取和测定工艺进行优化,以建立在室内筛选高产细胞系和试管苗株系的方法;【方法】采用超声波提取技术,对提取液、浸泡时间、提取温度等进行优化;【结果】将样品以75%甲醇浸泡6h后在32℃下超声提取两次,比色法测定总黄酮含量,加样回收率为95.3%～101.4%,相对标准偏差为1.61%。测得甘草初代愈伤组织中总黄酮含量为0.0012%～0.05%,子叶愈伤组织调控后含量高达0.08%,试管苗中总黄酮含量为1.04%～1.31%,移栽后高达4.5%。【结论】此工艺准确度较高、稳定性好、简便可行,为优良细胞系的筛选和试管苗的早期鉴定奠定了技术基础。 相似文献
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[目的]为实现植株中磷元素的快速监测,考察了不同消解前处理对试验结果的影响。[方法]通过比较不同消解方法的准确度、精密度、试剂消耗量和所用时间,得出各方法的相对优劣。[结果]以植株标准物质GBW10049、GBW10048和GBW07603为参照对象,微波消解和湿法消解法的结果误差分别为-0.12～0.05和-0.19～0.01,符合质控误差范围要求,采用干灰化法消解样品时,测定结果偏低,相对误差达到了8.2%。在植株中分别添加2个水平磷,微波消解法开展试验时平均回收率为102.3 %～108.3 %,相对标准偏差小于4.4%。文章还对影响光谱学性质的因素进行了讨论和优化,最终选择20 min为显色时间。[结论]在最优条件下,磷浓度在0.1～1.0 μg/mL范围内具有良好的线性,线性相关系数大于0.9990,微波消解-分光光度法能用于实际植株样品中的检测。 相似文献
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《Medicinal Plant》2014,(2)
[Objective]To measure the heavy metals and trace elements in 11 Rhizoma chuanxiong samples collected from 5 representative producing areas and perform correlation analysis. [Methods]The contents of the heavy metals including Hg,Cd,Pb,As,Cu,Zn,Se,Mn and Fe and the trace elements in the Rhizoma chuanxiong samples tested were measured by inductively coupled plasma optical emission spectrometer( ICP-OES),then the correlation between heavy metals,trace elements and producing region was analyzed by partial least squares using software simca- P +. [Results] The regression equations for each element detected by ICP-OES all showed a good linear relationship, with the correlation coefficient between 0. 999 7- 1,and recovery of 90. 02%- 108. 02%. [Conclusions]The microwave digestion-ICP-OES established in this study is simple,efficient and accurate,and can be used for the determination of heavy metals and trace elements in Rhizoma chuanxiong. The contents of heavy metals and trace elements are to some extent correlated with producing regions,in detail,the content of Fe, Mn,Zn and Cu showed an ascending trend in traditional Rhizoma chuanxiong producing regions. To conclude,it is suggested to periodically monitor the soil condition in traditional Rhizoma chuanxiong producing regions,and the pollution of heavy metals caused by climate. 相似文献
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《Medicinal Plant》2015,(Z1)
[Objective] To conduct simultaneous determination of selenium and germanium content in traditional Chinese medicine,such as wild RHIZOMA GASTRODIAE,RADIX POLYGONI MULTIFLORI,RADIX ANGELICAE SINENSIS,RADIX ADENOPHORAE,and RHIZOMA COPTIDIS,by hydride generation-atomic fluorescence spectroscopy. [Methods] The samples were prepared by nitric acid-hydrogen peroxide microwave digestion,using phosphoric acid as the medium and thiourea as the pre-reductant,the selenium and germanium contents of traditional Chinese medicine were determined by dual channel atomic fluorescence spectrometry. [Results] Under the optimized conditions,the instrumental detection limits of selenium and germanium were 0. 25 and 1. 46 ng / m L,and the RSDs were 1. 9%( 5 ng / m L,n = 10) and2. 9%( 10 ng / m L,n = 10),and the recoveries were 91. 7%- 97. 2% and 89. 5%- 95. 7% with RSD less than 4%( n = 10),respectively.The linear ranges were all 0- 300 ng / m L. Interference ions as Cr3 +,Cu2 +,Zn2 +and Co2 +had the biggest influences on selenium,while Pb2 +and Ni2 +the biggest influences on germanium. These interference ions were not considered,because their contents were not high enough to disturb selenium and germanium determination. [Conclusions]The selenium contents of 5 kinds of medicinal materials were in the range of533. 1- 1 773 ng / g,the order was: wild RHIZOMA GASTRODIAE RHIZOMA COPTIDIS RADIX POLYGONI MULTIFLORI RADIX ANGELICAE SINENSIS RADIX ADENOPHORAE; the germanium content range was 253. 9- 842. 5 ng / g,the order was: wild RHIZOMA GASTRODIAE RADIX ADENOPHORAE RADIX ANGELICAE SINENSIS RADIX POLYGONI MULTIFLORI RHIZOMA COPTIDIS. Two element contents of wild RHIZOMA GASTRODIAE were higher than that of other cultivated samples. 相似文献
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Hong Shuai Zhang Ze Zhang Lifu Ma Lulu Hai Xingyan Wang Zhen Zhang Hui Lü Xin 《棉花学报》2019,31(2):138-146
[Objective] This article is aimed to estimate the chlorophyll content of cotton canopy leaves in drip irrigation fields at different growth stages in northern Xinjiang and establish a model for estimating chlorophyll content in growth time series by using hyperspectral. [Method] Using Xinluzao 45 as the experimental material, the chlorophyll content and the corresponding spectral reflectance of cotton canopy leaves at different nitrogen levels and growing stages were measured, and the relationship between 12 indices and the chlorophyll content was analyzed. The estimation models of the chlorophyll content in cotton canopy leaves under drip irrigation were established. [Result] The correlation coefficient between the chlorophyll content of canopy leaves and Vogelmann red edge index was high in the four growing periods of cotton, and the correlation coefficient was 0.944, 0.907, 0.895, 0.930, respectively. And the spectral reflectivity was the highest at the flowering and boll period. The precision of the model established by the multivariate regression method is higher than that of the single exponential linear model with the determination coefficient more than 0.8 and the root mean square error smaller than that of the single exponential linear model. The model of the budding stage (y＝82.509x1＋89.937x2－94.438) has the best precision. [Conclusion] The chlorophyll content can be estimated by the models established at different growth stages, and the budding stage model has the best monitoring effect. 相似文献
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《Medicinal Plant》2014,(6)
[Objective]To investigate the preventive protective effects and its mechanism of vitamin C and polysaccharide in Dioscrea pposite Thunb. on cadmium-induced liver damage in mice. [Methods]50 Kunming mice were randomly divided into control group,model group,D. pposite polysaccharides protection group,vitamin C protection group,D. pposite polysaccharides and vitamin C protection group. At the 7th day of feeding,the mice were given saline by gavage,and the mice in other groups were intraperitoneally injected with 3. 0 mg /kg CdCl2 solution. 24 hours after injection,blood was collected for ALT and AST test. Then,mice were sacrificed and the livers were removed for the calculation of hepatosomatic index(liver weight / body weight). Also,the liver homogenates were prepared for the determination of SOD and GSHPx activities and the of MDA and NO contents. [Results]Compared with the mice in model group,the liver-somatic index,serum ALT,AST activity,and contents of liver MDA and NO reduced,the activities of SOD and GSH-Px,and the content of GSH improved in D. pposite polysaccharides and vitamin C protection group(*P 0. 05,**P 0. 01). Compared with the model grfoup,the protective effects of D. pposite polysaccharides combined with vitamin C strengthened(**P 0. 01). [Conclusions]The combination of D. pposite polysaccharides and vitamin C has preventive protection effects on cadmium-induced liver injury in mice by antagonizing cadmium and /or oxidative stress. The application of D. pposite polysaccharides combined with vitamin C had a synergistic effect. 相似文献
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[Objective]We studied the process of pigment gland formation and the dynamic change of gossypol synthesis, the dynamic relationship and molecular mechanism between the pigment glands and gossypol during the process of embryo formation and seed germination in G. bickii Prokh. [Method] We chose G. bickii as meterial and studied the tissue section observation, determination of gossypol contents and gene expression profile analysis of pigment gland formation and gossypol synthesis genes. [Result] The results showed that a large number of pigment protoglands formed on the cotyledons during embryo development process in G. bickii. The pigment protoglands formed to cavity structure at 12 hours after seed germination, and then converted into mature pigment glands. Analysis of gene expression profiles related to the pigment gland formation and gossypol synthesis during seed germination showing that GoPGF gene was highly expressed on the cotyledons of seed germination of 12 hours, which may be related to pigment protoglands forming to cavity structure of G.bickii. Farnesyl pyrophosphate synthase gene (FPS) was highly expressed on the cotyledons of seed germination of 24 hours showing it may be associated with the gossypol transported into the cotyledon pigment glands. CYP706B1 gene was highly expressed on the cotyledons of seed germination of 0 hours and HMG1 were highly expressed on the cotyledons of seed germination of 0 hours, 36 hours and 48 hours showing these genes may be associated with the formation of pigmented cavities and the storage and transport of gossypol; Cadinene synthase genes(CAD1-A) and HMG2 genes were not closely related to the pigment gland formation. [Conclusion] The 12 hours after seed germination is the key period of the morphogenesis of the pigment glands, and the morphogenesis of the pigment glands is synchrony with the time of the occurrence of gossypol in G.bickii. GoPGF, FPS, CYP706B1 and HMG1 are associated with the formation of pigmented cavities and the storage and transport of gossypol. 相似文献