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1.
The purpose of the present study was to investigate the influence of strain of Holstein-Friesian (HF) cow and feeding system (FS) on the lactation curve characteristics of spring-calving cows. The Wilmink model was used to analyse the lactation curves. The three strains of HF cows compared were high production North American (HP), high durability North American (HD) and high Breeding Worth New Zealand (NZ). The three FS compared were a high milk output from pasture feed system (MP), a high concentrate feed system (HC) and a high stocking rate feed system (HS). A repeated measures model with a factorial arrangement of treatments was used to determine the influence of strain of HF, FS, parity and their interactions on the shape of the lactation curve. The curve was described based on yield at calving, the degree of ascendancy between calving and peak yield, and the persistency after peak yield. Analysis of the residuals indicated a good fit of the Wilmink curve to the data set. Strain of HF, FS, parity and the interaction of strain of HF with FS had significant effects on lactation curve characteristics. In all three FS, the HP strain achieved the highest milk production post-claving and peak yield, with the lowest persistency of lactation. In the HC system, milk production post-claving and at peak yield were higher for all three strains. Offering higher levels of concentrate supplementation to the HP strain on a pasture-based system improved their persistency of lactation. The highest persistency of lactation was achieved with NZ strain. The highest milk production post-claving and at peak and lowest persistency was achieved with third parity cows. The existence of strain by feed system interactions for lactation curve parameters clearly exhibits that the optimum system of production varies with strain of HF.  相似文献   

2.
This study had two aims. Firstly, we wanted to quantify the effects of breed and parity on lactation curves. A parametric model for describing milk yield for cows [Friggens, N., Emmans, G., Veerkamp, R., 1999. On the use of simple ratios between lactation curve coefficients to describe parity effects on milk production. Livest. Prod. Sci. 62, 1–13] was used. The data contained 155,051 daily records of milk yield from 318 cows of three different breeds; Danish Red, Danish Holstein and Jersey. There were 276, 230, and 98 lactation curves for parities 1, 2 and 3 respectively. For every cow lactation, the parameters of the model were estimated using a least squares procedure for non-linear models. The resulting parameters were analysed in a mixed linear model. Significant effects of parity were observed on the same two parameters as in Friggens et al. [Friggens, N., Emmans, G., Veerkamp, R., 1999. On the use of simple ratios between lactation curve coefficients to describe parity effects on milk production. Livest. Prod. Sci. 62, 1–13]. Breed was also found to have a significant effect on some of the parameters. However, there was no significant interaction between breed and parity. The second aim of the study was to evaluate the properties of acceleration in milk yield in the context of providing an indicator for physiological stress and subsequent health problems. Milk yield acceleration was highest around calving and also reflected trends for higher stress/risk for higher yielding cows.  相似文献   

3.
A two‐dimensional random regression model with regressions on days in milk (DIM) and parity number was applied to lactational milk yields in Chinese Simmental cattle. Random regressions were fitted for additive genetic and permanent environmental effects using a two‐dimensional polynomial on DIM and parity number. A total of 4340 lactational milk yields from Chinese Simmental cattle which calved between 1980 and early 2000 were used in this study. Variance components were estimated using Bayesian methodology via Gibbs sampling. Variances of random regression coefficients associated with all terms of the polynomials were significant. A covariance function showed that heritabilities of lactational milk yields between 200 and 400 DIM over parities varied between 0.25 and 0.45. Heritabilities of 305‐day milk yields from 1st to 6–8th parities were 0.28, 0.30, 0.32 0.32, 0.32, and 0.31, respectively. Ratios of permanent environment variances to total variances at each DIM were greater than corresponding heritabilities. Generally, genetic correlations were higher between lactational milk yields with similar DIM and parity number.  相似文献   

4.
K. Togashi  C.Y. Lin   《Livestock Science》2007,110(1-2):64-72
First lactation milk production of Japanese Holstein cows was partitioned into ten stages. A quartic Legendre polynomial (k = 5) under an animal model was used to estimate the additive genetic covariance matrix between these ten stages. Various selection indexes with or without restrictions were constructed using the eigenvectors of the genetic covariance matrix. These eigen indexes were designed to improve lactation milk and persistency defined as difference in milk between DIM 280 and DIM 55. Two sets of economic weights between lactation milk and persistency and three levels of restriction on the intended gains in persistency were applied. The unrestricted index based on the first three eigenvectors achieved more than 99.9% of the net merit of the unrestricted index based on the first five eigenvectors. The advantage of unrestricted index over conventional selection based on lactation EBV increases with increasing economic weight assigned to persistency. The construction of the restricted eigen index does not require economic values between lactation milk and persistency and thus is more practical than the unrestricted eigen index which requires information on the economic values of both. The index coefficients of the eigen indexes reveal the relative selection emphasis among various stages of the lactation. When the degree of restriction or economic value for persistency increases, a greater selection emphasis was placed on the later part of the lactation than on the early part in order to realize the desired persistency. The developed eigen indexes provide useful information and easy understanding of the selection practice. A numerical example was given to illustrate the construction of the restricted eigen index.  相似文献   

5.
本研究通过对规模化牧场测定日数据进行分析,旨在估计不同地区305 d校正产奶量对应泌乳天数(DIM)的校正系数与胎次校正系数。利用Wood(不完全伽玛)模型对来自不同温度带9个参考群11 749头荷斯坦奶牛2010-2020年间的683 160条测定日产奶量拟合泌乳曲线,估计泌乳曲线参数,计算头胎牛和经产牛1~305 d泌乳天数对应的校正系数,分别分析参考群和验证群305 d校正产奶量和305 d实际累积奶量的差异;利用SAS 9.2中混合线性模型对1~6胎至少有前5个胎记录的牛估计305 d奶量的胎次效应值,并计算1~6胎各胎次的乘法校正系数;比较传统系数和新系数校正结果的差异。结果表明:①参考群头胎牛和经产牛中,泌乳曲线方程的拟合度R2范围分别为0.4593~0.4913和0.5796~0.6341,泌乳高峰日分别为79~85和53 d,泌乳高峰奶量分别为33.1~34.4和46.0~48.6 kg;②对于参考群,头胎牛和经产牛校正系数分别在泌乳≥90 d和泌乳≥30 d基本趋于一致,头胎牛和经产牛泌乳≥60 d的305 d校正产奶量均趋近于305 d实际累积奶量,奶量差值分别在100和200 kg以内;③对于验证群的305 d校正产奶量,头胎牛泌乳≥150 d和经产牛泌乳≥180 d的泌乳天数校正系数适用性更佳,305 d校正产奶量与305 d实际累积奶量比值高于79%;④1~6胎的胎次校正系数分别为1.2121、1.0380、1.0063、1.0000、1.0220和1.0290;⑤与传统系数相比,新泌乳天数校正系数校正效果更好,头胎牛和经产牛新系数校正的305 d奶量较305 d实际累积奶量相差分别在900和700 kg以内;利用新胎次校正系数,2~4胎产奶量可较为准确地校正到5胎成年当量。本研究结果表明,定期更新305 d校正产奶量的泌乳天数与胎次校正系数,便于准确地将不同泌乳天数、不同胎次状态下泌乳牛的产奶量调整到同一基准上,从而更好地比较奶牛个体泌乳性能的高低,为牧场管理提供参考依据。  相似文献   

6.
We examined the relationships between the shape of the first parity lactation curve and udder disease incidence at different stages of lactation in 538 Holstein cows. Data used were first‐parity daily milk yields and treatment records. Each cow was classified according to whether or not it had had udder disease at least once over the whole lactation period or in one of three stages within the lactation period. We then examined the differences in the shapes of the lactation curves between the disease incidence and non‐incidence group in each stage. Cows that had high rates of increase in milk yield and high milk yields in early lactation were predisposed to udder disease afterwards. Cows with high milk production over a long period but with low lactation persistency were predisposed to udder disease after the peak of lactation. There was no difference in total milk yield between incidence and non‐incidence groups in all stages, suggesting that, for a comparable level of lactation, cows without udder diseases have flatter lactation curves.  相似文献   

7.
Weekly milk records of 47 she-camels in a multibreed dairy camel herd were collected for over a period of 5 years. A total of 72 lactation curves were defined, and relationships with parity, calving season, lactation length, milk production level, following lactations, and dam weight were analyzed. Overall mean values were milk yield up to 12 months, 1,970?±?790 l; lactation length, 12.5 months; persistency, 94.7 %; weekly peak yield, 50.7 l; monthly peak yield, 220?±?90 l; and the number of weeks to reach peak yield, 28. The highest productivity was recorded in summer with a weekly mean of 48.2?±?19.4 l, compared with 34.1?±?16.3 l in winter. The highest average yield recorded was for camels at sixth parity, whereas the highest weekly peak was at eighth parity, and highest persistency at fifth parity. Camels that calved during the cold months (November to February) were most productives, with the highest persistency, peak yield, and longest lactation length. Four types of curves were identified corresponding to different parities and milk yield levels. Based on these data, specific models for camels are proposed.  相似文献   

8.
高产荷斯坦奶牛泌乳曲线的特征及相关分析   总被引:4,自引:0,他引:4  
选取285胎次第三、四胎305d产奶量超过8000kg的安徽荷斯坦牛的泌乳资料,利用Wood模型(Yt=ate^b-ct)进行高产荷斯坦奶牛泌乳曲线特征及相关的分析。结果表明:按照达到泌乳高峰周时间的不同对选取奶牛进行分级,11组Wood模型拟合泌乳曲线的平均拟合精度(R^2)为0.939(幅度为0.899-0.962)。随着达到泌乳高峰周时间的推迟,规模因子a具有逐渐降低的趋势;而产奶量上升率b、产奶量下降因子c、泌乳持久力表现与a相反的变化趋势。在相关分析中,MY(实际产奶量)与EMY(估计产奶量)、PY(高峰月产奶量)为强正相关,r(相关系数)分别为0.999和0.776,与持久力为中等正相关(r=0.379),与a、b、c的r分别是0.905、-0.875、0.731,与PY的相关程度较弱(r=-0.140)。  相似文献   

9.
To analyze how infection with Mycobacterium avium subsp. paratuberculosis (MAP) affects the shape of lactation curves, a three-level hierarchical test-day model was set up with fat-corrected test-day milk yield (FCTM) as response. Milk samples from 6955 cows in 108 Danish dairy herds were tested with ELISA to detect antibodies against MAP. Optical densities (ODs) recorded on a continuous scale were standardized according to parity and stage of lactation. In addition to standardized ODs (stOD), seven fixed covariates, quadratic terms and first-order interactions were included in the model. Cow and cow nested in herd were included as random effects. Cows of first, second and higher parities were analyzed separately. The lactation curves after peak yield were significantly less persistent in young infected cows, where an increase of one stOD unit was associated with a depression of the milk yield per day through day 305 of 3.7 kg FCTM in first parity and 2.7 kg FCTM in second parity. In second-parity cows, the lactation curve also was both depressed through the entire lactation and more steep after 60 days in milk (DIM). In third and older parities, a significant effect of the quadratic term of stOD indicated exponentially increased losses with increased ODs.  相似文献   

10.
[目的]研究产犊季节、胎次及不同牛场对奶牛泌乳性能的影响情况,以期为提高奶牛的泌乳性能及牛奶质量提供可靠的理论依据。[方法]收集3个千头以上奶牛场共8 872次相关信息,用多因素方差分析法分析产犊季节、胎次和牛场对泌乳天数、305d校正奶量、305d脂肪产量、305d蛋白产量和全泌乳期产奶量5个泌乳性能的影响进行分析。[结果]不同产犊季节、胎次和牛场都极显著影响奶牛的5项泌乳性能(P<0.01)。夏季和秋季产犊奶牛的泌乳性能较为理想。夏季产犊奶牛的泌乳天数和全泌乳期产奶量最高,其他三个泌乳性能都位居第二;秋季产犊奶牛的305d校正奶量、脂肪产量和蛋白产量都显著高于其他三个季节(P<0.05),泌乳天数和全泌乳期产奶量也仅次于夏季。头胎牛的各项泌乳性能都显著高于其他胎次的奶牛(P<0.05),其次为2胎产犊奶牛。随着胎次的增加,泌乳性能的各项指标都有不同程度的降低。除了泌乳天数之外,A牛场的各项泌乳性能都最佳,且显著高于B、C两个奶牛场(P<0.05)。[结论]在饲养条件良好的情况下,本研究中三个牛场的奶牛适宜在夏、秋两个季节产犊,此时泌乳性能较好;此外,头胎产犊奶牛的泌乳性能在各胎次中最高。  相似文献   

11.
Genetic parameters were estimated for loge somatic cell count (LSCC) for the first three lactations of 31 236 Holstein/Friesian cows with 308 534, 236 277 and 206 729 test day yields in parities 1, 2 and 3, respectively. An animal random regression model was employed in the analyses using Gibbs sampling with each parity regarded as different traits. Linear and quadratic functions were fitted for the animal and permanent environmental effects respectively, using orthogonal polynomials. Daily heritabilities increased with days in milk (DIM) and averaged about 0.07 in all three parities. This increase in heritabilities with DIM was due to an increase in genetic variance and decreases in both permanent and residual environmental variances with DIM. Environmental effects have a large influence on LSCC in early lactation in all three parities. Within lactation, genetic correlations were highest between adjacent DIM but decreased as DIM got further apart. However, this decrease was slowest in parity one and greatest in parity three. The lowest correlation within lactation was 0.10 between DIM 7 and 305 in parity 3. Across lactations, genetic correlations were highest between parities 2 and 3, intermediate between 1 and 3, and lowest between 1 and 2. The genetic correlations computed for completed lactations were 0.69, 0.79 and 0.98 between parities 1 and 2, 1 and 3, and 2 and 3, respectively. Corresponding phenotypic correlations between parities were 0.38, 0.31 and 0.52, respectively. A test day model, accounting for these variations in heritabilities and genetic correlations, should result in a more accurate evaluation.  相似文献   

12.
产犊季节、胎次及牛场对荷斯坦牛泌乳性能的影响   总被引:1,自引:0,他引:1  
本研究收集了3个千头以上奶牛场共8872条相关信息,采用多因素方差分析法分析了不同产犊季节、胎次和牛场对泌乳天数、305d校正奶量、305d脂肪产量、305d蛋白产量和全泌乳期产奶量5个泌乳性能指标的影响。结果表明,不同产犊季节、胎次和牛场都极显著影响奶牛的该5项泌乳性能(P〈0.01)。夏季和秋季产犊奶牛的泌乳性能较为理想。夏季产犊奶牛的泌乳天数和全泌乳期产奶量最高,其他三个泌乳性能指标都位居第二;秋季产犊奶牛的305d校正奶量、脂肪产量和蛋白产量都显著高于其他三个季节(P〈0.05),泌乳天数和全泌乳期产奶量也仅次于夏季。头胎牛的各项泌乳性能都显著高于其他胎次的奶牛(P〈0.05),其次为2胎产犊奶牛。随着胎次的增加,泌乳性能的各项指标都有不同程度的降低。不同的牛场极显著地影响奶牛的泌乳性能(P〈0.01)。  相似文献   

13.
The first breeding value for udder health of a bull is based on the performance of his daughters in their first lactation. However, clinical mastitis (CM) is not a problem in first lactation only. Therefore, the objective of this study was to estimate genetic parameters for CM and somatic cell count (SCC) for the first three lactations of Dutch Holstein cattle. Data from 250 Dutch herds recording CM were used to quantify the genetic variation of CM in parity 1, 2, and 3, respectively. The dataset contained 35,379 lactations from 21,064 animals of different parities. Test-day SCC was available from all lactations. Somatic cell counts were log-transformed to somatic cell scores (SCS) and averaged over test-day records between 5 and 335, 5 and 150, and 151 and 335 days in milk. Variance components for CM and SCS were estimated using a sire-maternal grandsire model. The heritability for CM was approximately 3% in all parities. Genetic correlations between CM in consecutive lactations were high (0.9), but somewhat lower between parity 1 and 3 (0.6). All genetic correlations between CM and SCS were positive, implying that genetic selection on lower SCC will reduce CM-incidence. Estimated genetic correlations were stronger for SCS in the first half of lactation than in the second half of lactation. Selection indices showed that most progress could be achieved when treating CM in parity 1, 2, and 3 as different traits and by including SCS between 5 and 150 days in the udder health index.  相似文献   

14.
The objectives of this study were to determine the effects of parity on milk production, body condition change, periparturient health, and culling in Korean dairy herds. The data utilized included; milk yield, body condition score, cow parity, calving condition, periparturient disorders, culling, and reproductive status, which were recorded from 1290 calvings in eight dairy herds. The mean milk yield in cows over 305 days increased with increasing parity (p < 0.01). Cows with parities of 3, 4, and 5 or higher lost more body condition than those with a parity of 1 during month 1 of lactation (p < 0.01), and body condition recovery by cows with parities of 4 and 5 or higher was slower (p < 0.01) than recovery by cows with parities of 1, 2, or 3 until month 3 of lactation. The risk of retained placenta, metabolic disorder, and endometritis also increased with advancing parity (p < 0.05). Moreover, the incidence of ovarian cysts was lower in cows with a parity of one than in cows with greater parities (p < 0.01). Culling rate due to reproductive failure also increased with advancing parity (p < 0.01). These results suggest that parity increases milk yield, body condition loss during early lactation, the risk of periparturient disorders, and culling due to reproductive failure in dairy herds.  相似文献   

15.
We propose a semi-parametric model for lactation curves that, along with stage of lactation, accounts for day of the year at milk recording and stage of gestation. Lactation is described as having 3 different phases defined by 2 change points of which the second is a function of gestation stage. Season of milk recording is modelled using cosine and sine functions. As an application, the model is used to estimate the association between intramammary infections (IMI) dynamics as measured by somatic cell count (SCC) over the dry period and the shape of the lactation curve. Milk recording data collected in 2128 herds from England and Wales between 2004 and 2007 were used in the analysis. From a random sample of 1000 of these herds, smoothed milk production was used to test the behaviour of the model and estimate model parameters. The first change point was set at 60 days in milk. The second change point was set at 100 days of gestation or 200 days in milk when the latter was not available. Using data from the 1128 remaining herds, multilevel models were then used to model individual test-day milk production within lactations within herds. Average milk production at 60 days in milk for cows of parities 1, 2, 3 and greater than 3 were 26.9 kg, 31.6 kg, 34.4 kg and 34.7 kg respectively and, after this stage, decreases in milk production per 100 days milk of lactation were 3.1 kg, 5.1 kg, 6.3 kg and 6.7 kg respectively. Compared to cows that had an SCC below 200,000 cells/mL on both the last milk recording in a lactation and the first milk recording in the following lactation, cows that had an SCC greater than 200,000 cells/mL on their first milk recording after calving had an estimated loss of milk production of between 216 and 518 kg depending on parity. These estimates demonstrate the impact of the dynamics of SCC during the dry period on milk production during the following lactation.  相似文献   

16.
In this study,the statistical analysis data of 185 340 Holstein dairy cows daily yield records were collected from 2011 to 2014 years in Changji area of Xinjiang,five kinds of models (Wood,IQP,Wilmink,ML,AS models) were used for the first,second,third,fourth and all parities fitting lactation curve.The results showed that the Wood,IQP,Wilmink,ML,AS models fitting precision were within the range from 0.9562 to 0.9828,0.9467 to 0.9809,0.8671 to 0.9752,0.8752 to 0.9175,0.8775 to 0.9127;Milmink model was the best fitting model for the first parity lactation curve,Wood model was the best fitting model for the second,third,and fourth parities lactation curve.  相似文献   

17.
本研究对新疆昌吉地区荷斯坦牛2011~2014年185 340条日单产记录进行统计分析,分别采用5种模型(Wood、IQP、Wilmink、ML、AS模型)对第1、2、3、4胎及所有胎次泌乳曲线进行拟合。结果表明,Wood、IQP、Wilmink、ML、AS模型拟合精度范围分别在0.9562~0.9828、0.9467~0.9809、0.8671~0.9752、0.8752~0.9175、 0.8775~0.9127范围内;Milmink模型为第1胎次泌乳曲线最佳拟合模型,Wood模型为第2、3、4胎次泌乳曲线的最佳模型。  相似文献   

18.
The epidemiology and genetic variability of clinical mastitis were examined. The data consisted of 70,775 Finnish Ayrshire cows. All cows were from milk recorded herds and calved during 1983. Each cow was under observation from the date of calving for 305 days. Only clinical mastitis diagnosed and treated by a veterinarian during the farm visits were taken into account. The lactation incidence rate of clinical mastitis (LIR) was 5.4 %. The cows calving in April-May had the highest LIR, but the seasonal variation was relatively small. The LIR increased with parity from the first to sixth parity. The cows treated for parturient paresis, infertility or ketosis had a higher risk of clinical mastitis than cows not treated for these diseases. The LIR was higher in herds with a high milk production level. The highest odds ratio estimated from parameters of the logit model was 14.8. The heritability estimates for clinical mastitis on the binomial scale were 3.2 % in parity 1, 1.6 % in parity 2, 0.6 % in parity 3 and 4, and 0.8 % in all parities (corresponding to 19.7 %, 8.3 %, 2.6 % and 3.8 % on the normal scale). These estimates indicate sufficient assurance for progeny testing of bulls and some possibilities of genetic selection against clinical mastitis. Genetic correlations between clinical mastitis and 305-days milk yield were 0.39 in parity 1, 0.51 in parity 2, 0.18 in parity 3–4 and 0.58 in all parities. This means that the best sires for milk yield had daughters with a higher LIR for clinical mastitis than the other sires.  相似文献   

19.
This study was conducted to evaluate the relationship between milk score (MS) and litter weight at 70 d (LW) for four sheep breeds in the United States. Milk score is a subjective measure of milk production used to assess milk production of range ewes when milk yield cannot be quantitatively determined. Variance components for MS and LW were estimated for each of Targhee, Columbia, Rambouillet, and Polypay breeds. Data collected from 1990 through 2000 at the U.S. Sheep Exp. Stn. in Dubois, ID, were analyzed with an animal model using REML. There were 13,900 records of MS and LW for 5,807 ewes. Records were grouped according to parity as first, second, and greater (mature), and all records (lifetime). Estimates of heritability for MS were in the range of 0.05 to 0.18 for first, 0.01 to 0.27 for second, 0.05 to 0.10 for mature, and 0.08 to 0.13 for all lifetime parity groups. Estimates of genetic correlation between MS at first and second parities ranged from 0.74 to 1.00. Similarly, mature MS was highly correlated genetically with MS at first (0.83 to 1.00) and at second (0.60 to 1.00) parities, suggesting that additive genetic value for milking ability at maturity could be evaluated as early as at first parity. Heritability estimates for LW ranged from 0.00 to 0.18 over all breeds and parity groupings. The genetic correlation between LW at first and second parity groups ranged from 0.43 to 1.00. Estimates of genetic correlation between LW at first or second parity with mature LW were mostly high and positive, except for Targhee (-0.10) and Polypay (0.14) at first parity. Litter weight for mature ewes could be improved by selection at first or second parity. Estimates of genetic correlation at first parity between MS and LW were high (1.00) for Rambouillet and Polypay, and near zero for Columbia and Targhee. At second parity, estimates of genetic correlation between MS and LW were positive and moderate for Rambouillet and Polypay but more variable for Columbia and Targhee. Estimates of genetic correlation between MS and LW were mostly positive and may be favorable with smaller estimates of standard errors using all lifetime records rather than first or second parity records. Although estimates are variable, the average of the estimates of the genetic correlation suggests that LW can be improved by selecting ewes for favorable MS.  相似文献   

20.

The main objective of this study was to estimate genetic correlations between fertility and production traits in first, second and third lactations as well as between fertility traits measured in the same way at different ages. The fertility traits studied were: number of inseminations per service period, number of treatments for reproductive disturbances, interval between first and last inseminations, interval between calving and first insemination, and interval between calving and last insemination. Early milk production was measured as the average of the energy-corrected milk yield at the second and third monthly testdays in a lactation. The number of records was approximately 450 000, 350 000, 180 000 and 75 000 in the heifer period, first, second, and third lactations, respectively. A linear, trivariate model that included the effects of herd-year, year, month, age and sire of the cow was applied. To reduce the effect of ongoing selection, 305-days kg protein production in first lactation was included as a variate in all of the analyses. Correlations between the herd-year effects indicated that factors of herd-year level conducive to increased production had a tendency to increase the number of inseminations as well as the number of reproductive treatments, although there was an earlier start and termination of the insemination period. Genetic correlations between fertility traits and production were in the range of 0.2-0.4, all of them unfavourable and higher at later parities. The genetic correlations between fertility traits in the heifer period and the same traits in first lactation were 0.7. Genetic correlations between the first and second lactation varied between 0.7 and 0.9, and between the second and third lactation they were all 0.9 or higher. In conclusion, fertility and production traits need to be selected for simultaneously if fertility is going to be maintained along further genetic improvement on production, and such selection should include fertility results from lactating cows.  相似文献   

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