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1.
We tested the hypothesis that poultry by-product meal would produce a thermogenic response (an increased requirement for oxygen) resulting in an increased incidence of pulmonary hypertension with right ventricular failure and ascites in commercial broiler chickens.

Four treatment groups, each with three replicates of 40 chicks, were fed a commercial broiler starter to day 21, grower to day 35, and the following experimental diets after day 35: group 1, commercial chicken broiler finisher; group 2, commercial chicken broiler finisher with poultry by-product meal added to replace part of the soyabean meal; group 3, commercial chicken broiler finisher with poultry fat added to replace the animal-vegetable (AV) fat; group 4, commercial chicken broiler finisher with both poultry by-product meal and poultry fat added to replace soyabean meal and AV fat. On day 35, pen temperature was reduced to 15°C, and on day 42 to 12°C.

Mortality from ascites between days 35 and 56 was 11(9%) in group 2, 5(4%) in group 4 and 3(2.5%) in groups 1 and 3 The incidence of pulmonary hypertension, as measured by an increased right ventricle: total ventricle (RV:TV) ratio (RV:TV > 0.249) at processing on day 57, was higher in the groups receiving poultry by-product and poultry fat: 27(22.5%) in group 2, 26(21.7%) in group 3, and 20(16.7%) in group 4 compared to that of the controls 12(10%).

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2.
The present study was conducted to evaluate the effect of dietary folic acid on the growth performance, intestinal morphology, and intestinal epithelial cells renewal in post-weaning piglets. Twenty-eight piglets (weaned at day 21, initial body weight of 6.73 ± 0.62 kg) were randomly allotted to 4 treatments with 7 pens per diet and 1 piglet per pen. The piglets were fed the same antibiotic-free and zinc oxide-free basal diets supplemented with folic acid at 0, 3, 9, and 18 mg/kg for 14 days. The results showed that dietary supplementation with folic acid increased villus height (VH) (P = 0.003; linear, P = 0.001), VH-to-crypt depth (VH:CD) ratio (P = 0.002; linear, P = 0.001), villus surface area (VSA) (P = 0.026; linear, P = 0.010). The analyzed parameters ADG, serum urea nitrogen (BUN) content, VH, VSA, and serum folate (SF) concentration responded linearly to the dietary folic acid concentration when the dietary folic acid concentration was below 4.42, 5.26, 4.79, 3.47, and 3.53 mg/kg respectively (R2 = 0.995, 0.995, 0.999, 0.999, 0.872, P = 0.09, 0.07, 0.09, 0.09, 0.36, respectively), as assessed by a two-linear broken-line regression. Above these breakpoints, the response of ADG, VH, VSA, and SF plateaued in response to changes in dietary folic acid concentration. Moreover, dietary supplementation with folic acid significantly increased the lactase (P = 0.001; linear, P = 0.001) and sucrase activities (P = 0.021; linear, P = 0.010) in the jejunal mucosa of weaned piglets. The mRNA expression of solute carrier family 6 member 19 (SLC6a19), solute carrier family 1 member 1 (SLC7a1), tumor necrosis factor-α (TNF-α), the number of Ki67 positive cells, and cell shedding rate had a significant linear contrast (P = 0.023, 0.021, 0.038, 0.049, and 0.008, respectively) in dietary folic acid groups. In conclusion, our results indicate that folic acid supplementation can improve the growth performance and intestinal morphology of weaned piglets by maintaining the balance of epithelial cell renewal.  相似文献   

3.
This experiment aimed to examine the effect of periodical application of bioactive peptides derived from cottonseed (BPC) in comparison with using sub-therapeutic doses of lincomycin and the excessive inclusion of vitamin E on performance, immunity, total antioxidant capacity of serum and intestinal morphology of broiler chickens. A total of 240 one-d-old male broiler chicks with similar initial weight (Ross strain) were randomly assigned to 6 groups (8 chicks/pen): non-treated group (basal diet), basal diet supplemented with 2 mg/kg lincomycin, basal diet supplemented with 50 IU vitamin E, basal diet supplemented with 6 g BPC/kg in starter period, basal diet supplemented with 6 g BPC/kg in starter and grower periods and basal diet supplemented with 6 g BPC/kg throughout the whole experiment. The highest final body weight was obtained in the group supplemented with BPC in starter and grower periods. In the finisher phase, broilers fed the diet containing BPC in the starter period and in the whole trial had significantly (P < 0.05) better feed conversion ratios (FCR). Jejunal villus height was significantly elevated in broilers supplemented with antibiotic (P < 0.001), furthermore it tended to be greater in broilers fed BPC in the starter period. The jejunal villus height-to-crypt depth ratio was significantly (P < 0.01) higher in broilers fed the diet containing antibiotic in comparison to other groups. Humoral immune response against Newcastle disease vaccine tended to be elevated in broilers fed the diet containing BPC in the whole trial (P > 0.05). Broilers supplemented with BPC in starter and grower, and in the whole trial had significantly (P < 0.05) higher antibody titers against sheep red blood cells (SRBC). The highest total antioxidant capacity was obtained in broilers supplemented with the excessive level of vitamin E, furthermore it tended to improve in broilers fed the diet containing BPC in the whole trial. In summary, the results of the study indicated that addition of BPC in broiler diets in the whole trial could improve FCR, immune responses and total antioxidant activity of serum, and BPC could be used in broiler diets as an alternative to in-feed antibiotics.  相似文献   

4.
The present study aimed to investigate the effects of dietary pioglitazone hydrochloride (PGZ) and l-carnosine (LC) supplementation on the growth performance, meat quality, antioxidant status, and meat shelf life of yellow-feathered broiler chickens. Five hundred broiler chickens were randomly assigned into 4 experimental diets using a 2 × 2 factorial arrangement with 2 PGZ supplemental levels (0 and 15 mg/kg) and 2 LC supplemental levels (0 and 400 mg/kg) in basal diets for 28 d. The feed-to-gain ratio decreased whereas the average daily gain increased with PGZ supplementation. Greater dressing percentages, contents of intramuscular fat (IMF) in breast and thigh muscles, C18:3n-6, C18:1n-9 and monounsaturated fatty acid (MUFA) percentages of thigh muscle were observed with PGZ addition. Additionally, significant synergistic effects between PGZ and LC on the C18:1n-9 and MUFA contents were found. Supplementation with LC decreased drip loss, cooking loss and total volatile basic nitrogen, and increased the redness (a∗) value, the superoxide dismutase and glutathione peroxidase activities in thigh muscles. Moreover, the malondialdehyde content decreased when diets were supplemented with LC, and there was a synergistic effect between PGZ and LC. Additionally, the mRNA abundance of lipogenesis-related genes, such as peroxisome proliferator-activated receptor γ (PPARγ), PPARγ co-activator 1α and fatty acid-binding protein 3, increased with PGZ supplementation, and relevant antioxidation genes, such as nuclear factor erythroid-2-related factor 2 and superoxide dismutase 1, were enhanced with LC supplementation. In conclusion, the results indicated that the supplementation of PGZ and LC could improve the growth performance, antioxidant ability, IMF content, and meat shelf life of yellow-feathered broiler chickens.  相似文献   

5.
Fifteen adult male rats, the “experimental” rats, were fed a daily diet which contained methyl mercury hydroxide combined with liver homogenate. The daily dose of methyl mercury in terms of metallic mercury was 1.8 mg per rat. Six similar rats, the “controls”, were fed the same diet without methyl mercury. After the experimental rats had developed symptoms typical of methyl mercury poisoning, which occurred on the 15th day of the experiment, all the test animals were decapitated. The average total dose of methyl mercury producing toxic symptoms was 58,1 ± 6.1 mg Hg per kg body weight. The sulfhydryl groups and the mercury content of the brains, livers and caudal femoral muscles of the animals were determined. The SH determinations were made by amperometric titration and the mercury determinations by neutron activation analysis.The mean number of SH groups in the brains (14.95 ± 1.97 µM/g) and livers (35.89 ± 4.10 µM/g) of the methyl mercury-fed rats was found to be significantly (P < 0.001 and P < 0.01 respectively) lower than the corresponding means of the livers (17.63 ± 1.12 µM/g) and brains (44.75 ± 5.60 µM/g) of the controls. The number of SH groups in the muscles did not differ significantly between the animal groups (0.5 < P < 0.6). The mean mercury content of the brains, livers and muscles was found to be 26.0, 124.6 and 39.1 p.p.m. respectively, corresponding to 0.12, 0.62 and 0.19 µM per g of tissue. The decrease in the number of SH groups in the brains, 2.68 µM/g, and in the livers, 8.86 µM/g, of the methyl mercury-fed rats is thus considered to be due mainly to other effects of methyl mercury than to the direct binding of this compound to the tissue SH groups.  相似文献   

6.
The work describes the effects of cimetidine on stress-induced gizzard erosions (Experiment A) and the influence of the long-term application (42 days) of the same drug on weight gain and feed consumption during broiler fattening (Experiment B). For Experiment A, 60 male, three-day-old chicks were divided into two groups: C (n = 30)--control chicks treated with 0.5 ml saline; CIM (n = 30)--chicks treated with cimetidine in a dose of 5 mg/kg body weight (b. w.) intragastrically. All chicks were stressed using a modified water-immersion stress method according to which the chicks, after 24 h of feed deprivation, were immersed in tap water (17 degrees C) for a few seconds. Under chloroform anaesthesia ten chicks from each group were killed 1, 2 and 3 h after the stressing. The morphometric analysis of gizzard erosion (GE) and histopathological examinations of gizzards were performed for each chick. In Experiment B, 32 one-day-old broilers of both sexes were used. The control group was untreated (n = 16) while the CIM group (n = 16) was fed the same diet supplemented with 10 mg of cimetidine per kilogram of feed throughout the fattening period (42 days). The results of Experiment A showed decreased mean length of the GE in the cimetidine-treated birds as compared with the GE lesions of the controls. In Experiment B, the treated chicks had reduced liveweight (1835.1 g), carcass weight (1474.6 g) and increased feed consumption (2115 g of feed per kilogram of weight gain) compared to the controls in which the same parameters were 1898.5 g, 1574.2 g and 1797 g, respectively. The results show that while stress-induced GE of chicks can be medicated pharmacologically, long-term application of the same substance impairs the results of fattening.  相似文献   

7.
Two experiments were conducted with Ross × Ross broiler chicks in battery brooders from 7 to 21 d of age to determine the Arg and Met requirements of young broiler chicks at control (25°C) and warm (35°C) temperatures. In both experiments, 1-d-old broiler chicks were fed a corn and soybean meal based starter diet for 7 d. Six replications (2 replicates in each of 3 rooms per temperature) with 8 chicks each were used for each treatment. In experiment 1, the basal diet was based on corn (34.52%), whey (26.96%), corn gluten meal (16.53%), soybean meal (11.74%), and poultry fat (23% of CP and 3.20 kcal/g of MEn). Six levels of Arg (0, 0.1, 0.2, 0.3, 0.4, and 0.5%), supplementing the basal diet containing 0.95% Arg, were the dietary treatments. A broken-line linear model was used to estimate chick Arg requirements. Based on body gain and feed conversion ratio (FCR) data, respectively, the Arg requirements of young Ross × Ross broiler chicks raised at 35°C were 1.15 ± 0.03% and 1.13 ± 0.02%, whereas those of chicks at 25°C were 1.26 ± 0.03% and 1.27 ± 0.02%. In experiment 2, the influences of temperature and dietary Arg on the Met requirements of young broiler chicks were investigated. The basal diet was based on corn (53.45%), soybean meal (37.72%), and poultry fat (23% of CP and 3.20 kcal/g of ME). Experiment 2 had a 6 × 2 factorial arrangement, with the basal diet (0.35% Met and 1.52% Arg) supplemented with 6 levels of dietary Met (0, 0.05, 0.1, 0.15, 0.2, or 0.3%) and 2 levels of dietary Arg (0 and 1.0%). When chicks were fed a corn-soybean meal basal diet containing 1.52% Arg, the Met requirements of young Ross × Ross broiler chicks raised at 35°C were 0.43 ± 0.02% and 0.43 ± 0.03%, whereas those of chicks at 25°C was 0.43 ± 0.01% and 0.48 ± 0.03%, based on body gain and FCR data, respectively. When Arg levels were increased to 2.52%, the Met requirement of young Ross × Ross broiler chicks was greater at both temperatures (P < 0.05). The requirements of chicks raised at 35°C were 0.50 ± 0.02% and 0.49 ± 0.02% and at 25°C were 0.59 ± 0.03% and 0.57 ± 0.02%, based on body gain and FCR data, respectively. Temperature and amino acid balance may both affect the amino acid requirements of broilers.  相似文献   

8.
Pathology and histopathology of gossypol toxicity in broiler chicks   总被引：1，自引：0，他引：1  
Two experiments were conducted to determine the toxicity, pathology, and histopathology of purified gossypol in broiler chicks. Gossypol was added to broiler feed at 0, 100, 200, and 400 mg/kg of feed in Experiment 1 and at 0, 800, and 1600 mg/kg of feed in Experiment 2. Day-old broiler chicks were fed these diets from 1 to 21 days in Experiment 1 and from 1 to 23 days in Experiment 2. In Experiment 1, body weight and feed intake at 21 days were not significantly affected by dietary gossypol. However, chicks fed gossypol at 400 mg/kg of feed had poor feed conversion ratio compared with the other treatment. Feed conversion ratios were 1.493, 1.564, 1.471, and 1.60 for chicks fed gossypol at 0, 100, 200, and 400 mg/kg of feed, respectively (Experiment 1). Chicks fed 400 mg/kg gossypol also had mild perivascular lymphoid aggregate formations and bilary hyperplasia in the liver. In Experiment 2, gossypol at 1600 mg/kg resulted in 28.1% mortality. Gossypol at 800 and 1600 mg/kg feed resulted in significant decreases in body weight and feed intake of chicks. The average body weights of 23-day-old chicks in Experiment 2 were 676, 224, and 111 g for 0, 800, and 1600 mg/kg gossypol, respectively. Feed conversion ratios of chicks fed 800 and 1600 mg/kg gossypol were significantly higher than those of chicks fed control diets (1.383 vs. 1.564 vs. 1.745 for 0, 800, and 1600 mg/kg gossypol, respectively). Plasma iron and hematocrit values were significantly reduced by gossypol at 800 and 1600 mg/kg of feed. Enlarged gallbladder was the only gross pathology symptom associated with gossypol levels. Severe cases of perivascular lymphoid aggregate formation, biliary hyperplasia, and hepatic cholestasis were observed in chicks fed 800 and 1600 mg/kg of gossypol in feed. No gossypol-related changes were observed in kidney tissues of chicks. These results show that gossypol is toxic to broiler chicks at high levels. This study also shows that histopathologic changes in liver due to gossypol also occur at levels lower than the levels that affect body weight.  相似文献   

9.
Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%).

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-015-0234-8) contains supplementary material, which is available to authorized users.  相似文献   

10.
We conducted 3 independent experiments to demonstrate functional G-coupled protein receptor 43 (GPR43) and GPR120 in bovine intramuscular (i.m.) and subcutaneous (s.c.) adipose tissues. We hypothesized that media volatile fatty acids and long-chain fatty acids would affect cAMP-activated protein kinase-alpha (AMPKα) protein expression and cAMP concentrations differently in i.m. and s.c. adipose tissue. Experiment 1: oleic acid (18:1n-9) decreased phosphorylated AMPKα protein (p-AMPKα) and the p-AMPKα/AMPKα protein ratio in i.m. preadipocytes, increased the p-AMPKα/AMPKα protein ratio in bovine satellite cells, and had no effect in s.c. preadipocytes. Experment 2: ex vivo explants from the 5th to 8th longissimus thoracic rib muscle section of Angus crossbred steers were cultured 48 hr in media containing 0.25 µM ciglitizone, 5 mM glucose, and 5 mM acetate, in the absence or the presence of 100 µM oleic acid. Oleic acid increased acetate incorporation into fatty acids and GPR43 gene expression in i.m. adipose tissue (P < 0.05), but oleic acid had no effect on fatty acid synthesis or GPR43 expression in s.c. adipose tissue. Experiment 3: fresh s.c. and i.m. adipose tissue from the 5th to 8th longissimus thoracic rib muscle section of Angus crossbred steers was transferred immediately to 6-well culture plates containing 3 mL of KHB/Hepes/5 mM glucose. Samples were preincubated with 0.5 mM theophylline plus 10 μM forskolin for 30 min, after which increasing concentrations of acetate or propionate (0, 10−3, 10−2.3, and 10−3 M) in the absence or the presence of 100 μM oleic acid or 100 µM palmitic acid (16:0) were added to the incubation media. Acetate had no effect on forskolin-stimulated cAMP production in s.c. adipose tissue but decreased cAMP in i.m. adipose tissue (P < 0.05); this indicates a functional GPR43 receptor in i.m. adipose tissue. The combination of 10−2 M acetate and oleic acid decrease cAMP production in s.c. adipose tissue, consistent with GPR120 receptor activity, but oleic acid and palmitic acid attenuated the depression of cAMP production caused by acetate in i.m. adipose tissue. Palmitic acid depressed cAMP production in s.c. adipose tissue, and increased cAMP production in i.m. adipose tissue (P < 0.05). Propionate had no effect on cAMP production in s.c. or i.m. adipose tissue. These results provide evidence for functional GPR43 receptors in i.m. adipose tissue and GPR120 receptors in s.c. adipose tissue, both of which would suppress lipolysis.  相似文献   

11.
ABSTRACT

1. The present study was conducted to evaluate the effects of conditioning times and processed sodium bentonite (PSB)-based pellet binder (G. Bind?) on pellet quality, performance, small intestine morphology, and nutrient retention in growing broiler chickens (d 11–24).

2. A total of 540, one-day-old male broiler chicks were fed a commercial starter diet (d 1–10). On day 11, birds were assigned to a 2 × 3 factorial arrangement including two conditioning times (2 and 4 min) and three levels of PSB (0, 7.5 and 15 g/kg) with six replicates of 15 chicks each. Feed intake and weight gain were recorded to calculate growth performance. The jejunal segment and excreta samples were collected to determine intestinal morphology and nutrient retention, respectively.

3. Diets produced with 2 min conditioning time and containing 15 g/kg PSB increased (P < 0.05) pellet durability index and hardness. Conditioning time and PSB levels had no significant effect on growth performance. The inclusion of PSB to broilers diet increased (P < 0.05) energy usage of the pelleting machine. Chickens fed the diet conditioned for 2 min and containing 15 g/kg PSB had the lowest (P < 0.05) relative jejunal length. Two minutes conditioning of diets containing 15 g/kg PSB increased (P < 0.05) apparent metabolisable energy retention in broilers.

4. It was concluded that 2 min conditioning of diet containing 15 g/kg PSB improved pellet quality and nutrient retention of broiler chickens, while the main effects of conditioning time and PSB levels were controversial in most evaluated parameters.  相似文献   

12.
The aim of this study was to evaluate the effects of dietary betaine (BET) on growth performance, redox state, and related gene expression in broilers under heat stress (HS). A total of 144 21-day-old male broiler chickens with similar body weights were assigned randomly to three treatments with six replicates (eight chickens per replicate cage). Broilers in the control (CON) group were kept at thermoneutral (TN, 22±1°C) conditions and fed a basal diet until they were 42 days of age. Broilers in the other two groups (defined as HS and HS + BET) were exposed to HS (34±1°C, 8 h/day) and fed the basal diet without or with 1000 mg/kg BET, respectively. Rectal and cockscomb temperature of broilers was increased (P<0.05) in HS and HS + BET groups compared with the CON group, whereas there was no difference between HS and HS + BET groups. Dietary BET supplementation restored (P<0.05) average daily gain (ADG) and average daily feed intake (ADFI) of broilers and reversed (P<0.05) the increase in serum alanine transaminase (ALT) activity and malondialdehyde (MDA) content in the liver tissue of broilers under HS. The HS + BET group had higher (P<0.05) activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the liver tissue and mitochondria than the HS group, and the same pattern was observed for glutathione (GSH) and GSH/glutathione disulphide (GSSG) in the liver tissue. The decreased mRNA levels of GPX1 and uncoupling protein (UCP) in the liver induced by HS were restored by BET supplementation. In conclusion, dietary BET supplementation can alleviate HS-induced hepatic and mitochondrial oxidative damage of broilers by regulating mRNA expressions of GPX1 and UCP.  相似文献   

13.
In this study, supplementation of two levels (5 and 25 parts per million; ppm) of boron into broiler diets including 125 kg−1(inadequate) and 2000 kg−1(adequate) vitamin D3as investigated. The effects of supplementation on performance and biochemical characters (Ca, P, Mg, glucose and ) of broilers from 1 to 45 days of age were evaluated. Boron provided significant increases in performances of chicks fed both adequate and inadequate vitamin D3-containing diets. The improvements in the inadequate vitamin D3-containing group were higher than that of adequate vitamin D3-containing group. The boron addition had a positive effect on Ca, P and alkaline phosphatase levels of chicks. Boron might be regarded as beneficial in inadequate vitamin D3-containing broiler feed.  相似文献   

14.
The aim of this study was to evaluate the effects of dietary brown rice on the growth performance, systemic oxidative status, and splenic inflammatory responses of broiler chickens under both thermo-neutral and chronic heat stress conditions. Forty 12-day-old male broiler chickens (ROSS 308) were randomly assigned to two groups and fed either a control diet (corn-based) or a brown rice-based diet. After seven days (19 days old), both groups were randomly divided into two sub-groups (n=10), one of which was exposed to heat stress (33°C for 14 days), while the other was maintained at 24°C. Heat exposure reduced the body weight gain and feed intake (p<0.01) of both groups. In terms of oxidative plasma states, heat exposure reduced the glutathione peroxidase activity and increased the ceruloplasmin content, while the 2-thiobarbituric acid reactive substance and reduced glutathione levels were not affected adversely. Heat exposure activated the immune responses, as evidenced by increased plasma immunoglobin levels, and altered splenic immune-related gene expressions including heat shock proteins, toll-like receptor 4, and interleukin-12. Under both thermo-neutral and heat stress conditions, dietary brown rice improved the growth performance, decreased the immunoglobulin levels, and down-regulated the expression of splenic immune-related genes of broilers, although their systemic oxidative status was not affected. Dietary brown rice should be considered as a valuable component of broiler chicken feeds subjected to both thermo-neutral and heat stress conditions. The positive effects of brown rice on bird performance may be associated with the modulation of the immune responses, as reflected by the decreased production of immunoglobulins and altered splenic immune-related gene expression.  相似文献   

15.
Glycation is a non-enzymatic reaction inducing the bonding of glucose to amino acids and proteins. Glycated amino acids are not useful for protein synthesis, suggesting that glycation reduces the utilization of amino acids. Metformin (MF) is well known as a therapeutic drug for type II diabetes that inhibits glycation. It is possible that treatment with MF raises the utilization of amino acids by the inhibition of glycation, thereby improving the growth performance of chickens. In the present study, therefore, we investigated the influence of dietary MF on the growth performance, and plasma concentrations of free amino acids and Nε-(Carboxymethyl)lysine (CML), which is an advanced glycation end product, in layer (Experiment 1) and broiler (Experiment 2) chickens. From 7 d of age, chicks were allowed free access to one of the experimental diets containing MF at 3 supplementation levels (0, 150, and 300 mg/kg diet) for 14 days. Body weight and feed intake were measured every week. At the end of the experiments, blood and breast muscle (M. pectoralis major) were collected for further analysis. Dietary MF did not affect weight gain, feed intake, or feed efficiency in both layer and broiler chickens. Dietary MF at the level of 150 mg/kg diet increased breast muscle weight in both layer and broiler chickens. Dietary MF increased plasma concentrations of branched chain amino acids and decreased concentrations of CML in layer chickens, although it did not affect plasma concentrations of glucose. The present study suggested that dietary MF might have the potency to increase breast muscle weight of layer chickens with an increment in plasma concentrations of branched-chain amino acids.  相似文献   

16.
The methylation status of pivotal genes involved in fat deposition in chickens has been extensively studied. However, the whole-genome DNA methylation profiles of broiler abdominal adipose tissue remain poorly understood. Using whole-genome bisulfite sequencing, we generated DNA methylation profiles of chicken abdominal adipose tissue from Northeast Agricultural University broiler lines divergently selected for abdominal fat content. We aimed to explore whether DNA methylation was associated with abdominal fat deposition in broilers. The whole-genome DNA methylation profiles of fat- and lean-line broilers abdominal adipose tissue were constructed. The DNA methylation levels of functional genomic regions in the fat broiler were higher than those in the lean broiler, especially in the 3′ untranslated regions (UTRs) and exons in the non-CG contexts. Additionally, we identified 29,631 differentially methylated regions and, subsequently, annotated 6,484 and 2,016 differentially methylated genes (DMGs) in the gene body and promoter regions between the two lines, respectively. Functional annotation showed that the DMGs in promoter regions were significantly enriched mainly in the triglyceride catabolic process, lipid metabolism-related pathways, and extracellular matrix signal pathways. When the DMG in promoter regions and differentially expressed genes were integrated, we identified 30 genes with DNA methylation levels that negatively correlated with their messenger RNA (mRNA) expression, of which CMSS1 reached significant levels (false discovery rate < 0.05). These 30 genes were mainly involved in fatty acid metabolism, peroxisome-proliferator-activated receptor signaling, Wnt signaling pathways, transmembrane transport, RNA degradation, and glycosaminoglycan degradation. Comparing the DNA methylation profiles between fat- and lean-line broilers demonstrated that DNA methylation is involved in regulating broiler abdominal fat deposition. Our study offers a basis for further exploring the underlying mechanisms of abdominal adipose deposition in broilers.  相似文献   

17.
1. A study was conducted to evaluate the effects of ochratoxin A (OA) on broiler chicks challenged with Salmonella gallinarum. 2. One hundred and seventy-six 1-d-old broiler chicks were divided into two groups of 88 chicks each, with one group fed on a control mash diet and the other given a mash diet containing 2 ppm OA. On d 14, each group was further subdivided into two groups with one group infected with S. gallinarum and the other uninfected. 3. Following S. gallinarum inoculation on d 14, 4 birds from each group were killed at 1, 2, 3, 5, 7, 10, 14 and 21 d post inoculation. 4. S. gallinarum infection caused dullness, depression, weakness, increased thirst, droopy wings, ruffled feathers and greenish-yellow diarrhoea. S. gallinarum infection in the absence of OA caused 11.5% mortality which increased to 28.8% in the presence of OA. 5. Decreased body weight and reduced feed intake were observed in chicks fed on the diet containing OA. S. gallinarum infection also reduced the body weights of chicks, with the effects being more marked in chicks receiving OA. The OA diet led to increased serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, uric acid and creatinine, and decreased levels of total proteins, albumin, globulins, calcium and phosphorus. S. gallinarum infection did not cause significant alteration in any of the serum biochemical parameters. 6. Mortality and the severity of S. gallinarum infection in broiler chicks were increased by the presence of OA in the diet.  相似文献   

18.
This study was carried out to evaluate the nutritional effects of rice feeding and carnitine addition to a diet for broiler chicks. Thirty‐six male 10‐day‐old broiler chicks were assigned to one of the following four treatment groups: corn‐based diet (corn group), rice‐based diet (rice group), and each diet with added carnitine (100 ppm). The experimental period was 2 weeks. Rice feeding resulted in significantly higher growth performance (body weight gain and feed efficiency) compared to corn feeding. Carnitine addition also resulted in higher growth performance. Breast muscle and thigh muscle weight (g) were significantly higher in broiler chicks fed rice and those fed diets with added carnitine. Liver mRNA expression of IGF‐I was significantly higher in broiler chicks fed rice compared to those fed corn. There was no significant difference in mRNA expression of muscle atrogin‐1 or liver CPT‐I between broiler chicks fed rice and those fed corn, not between broilers chicks fed diets containing carnitine or not. Overall, these results show that rice feeding and carnitine addition improve the growth performance of broiler chicks by increasing mRNA expression of liver IGF‐I. In addition, carnitine action is not affected by different cereals (corn and rice).  相似文献   

19.
Characteristics of 50 strains of G. jejuni/coli isolated from rectal swabs, caecal contents or bile of cow, sheep, goat, swine, broiler chicken and man were investigated. All the strains grew at 30 °C, 35°C 42°C, but not at 25 °C. The strains were nalidixic acid sensitive. Differences were noticed in salt-tolerance, in the growth with 2,3,5 tri-phenyltetracolium chloride and in the hippurate hydrolysis. Growth of the strains with certain bile salts was also investigated and the results are discussed.  相似文献   

20.
Two experiments were conducted with broiler chicks to determine the nutritive value of high-oil sunflower meal (HO-SFM), a sunflower oil extraction by-product obtained through screw-press extraction and expanding processes with a proximate composition of 32% CP, 12% crude fiber, and 19% ether extract. In Experiment 1, the effects of a high level (46.4%) of HO-SFM on chick performance and gastrointestinal organs were tested. The objective of Experiment 2 was to determine if pelleting the feed could overcome the bulkiness resulting from inclusion of HO-SFM at a high level. The results of Experiment 1 indicated that addition of 46.4% HO-SFM to broiler starter diets significantly (P < 0.05) depressed body weight, feed intake, and gain but not feed conversion. Fat pad and liver lipid were again significantly (P < 0.05) decreased in the HO-SFM treatment. Impaired performance might have been due to the difference of the density of HO-SFM diet (608 g/L) compared with the soybean meal control (723 g/L). When bulkiness was overcome by pelleting in Experiment 2, it was found that pelleting the feed significantly enhanced growth of broiler chicks compared with SFM or soybean meal mash diets. Liver weights and lipid content were again decreased in HO-SFM diets. The results of this study suggest that HO-SFM can be used up to 28% without adverse effects on broiler chicks. Further improvement was observed with pelleting. Liver weight and lipid content were consistently reduced by feeding HO-SFM.  相似文献   

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