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1.
《畜牧与兽医》2019,(12):25-29
试验旨在探究补饲颗粒料对断奶羔羊体重、肉品质及经济效益的影响,试验选取断奶湖羊公羔44只,随机分为对照组和试验组,试验羊只每天额外补饲200 g育肥颗粒料,试验期为90 d。测定试验期第0天、第30天和第90天的湖羊体重。试验结束屠宰采样测定湖羊背最长肌肉品质。结果显示,较对照组相比,补饲颗粒料可显著提高育肥湖羊3月龄重和5月龄重(P0.05),且极显著提高育肥羊总增重和平均日增重P0.01)。补饲颗粒料可显著提高5月龄湖羊背最长肌粗蛋白含量(P0.05),显著提高5月龄湖羊肌肉中谷氨酸、蛋氨酸、异亮氨酸、组氨酸、赖氨酸、精氨酸、必需氨基酸、非必需氨基酸和氨基酸总和的含量(P0.05)。另外,补饲颗粒料显著提高5月龄湖羊肌肉中EAA占TAA的百分比(P0.05)。试验组每只羊纯利润比对照组高62.1元,提高效益16.75%。结果表明,补饲颗粒料可提高湖羊生长性能,改善肉品质,增加经济效益。  相似文献   

2.
本试验旨在研究饲粮中添加甜菜碱和表没食子儿茶素没食子酸酯(EGCG)对育肥猪生长性能、肉品质、血清生化和抗氧化指标的影响。选取81头平均体重为90.03 kg的育肥期“杜×长×大”三元杂交猪,随机分为3组,每组3个重复,每个重复9头猪。对照组饲喂基础饲粮,试验Ⅰ组在基础饲粮中添加2 500 mg/kg的甜菜碱,试验Ⅱ组在基础饲粮中添加2 500 mg/kg的甜菜碱和1 000 mg/kg的EGCG。预试期5 d,正试期40 d。结果表明:1)与对照组相比,试验Ⅰ、Ⅱ组的平均日采食量均显著降低(P<0.05)。2)与对照组相比,试验Ⅰ、Ⅱ组的背膘厚和背最长肌24 h滴水损失均显著降低(P<0.05),试验Ⅰ组的背最长肌肌内脂肪含量显著升高(P<0.05)。3)各组之间背最长肌氨基酸含量均无显著差异(P>0.05)。与对照组相比,试验Ⅰ、Ⅱ组的背最长肌二十二碳酸含量均显著降低(P<0.05);各组之间背最长肌其他脂肪酸含量均无显著差异(P>0.05)。4)与对照组相比,试验Ⅰ组的背最长肌肌球蛋白重链Ⅱb(MyHCⅡb)的mRNA相对表达水平显著降低(P&...  相似文献   

3.
试验旨在研究不同蛋白营养水平精补料对隔栏补饲羔羊生长育肥性能的影响。采用随机分组试验设计,选择同期产羔母羊36只所生15日龄、体质量10 kg左右随母哺乳的滩寒杂种羔羊48只,随机分为4组,每组随母哺乳羔羊12只,每只羊为1个重复。羔羊与母羊隔栏补饲,羔羊精补料设4个蛋白营养水平,分别为13.10%、18.51%、23.84%和28.17%;哺乳母羊营养水平一致。结果表明:试验Ⅲ组净肉率极显著高于Ⅱ组(P0.01);Ⅰ组和Ⅱ组的GR值显著高于Ⅳ组和Ⅲ组(P0.05)。4个组的平均料重比排序依次是Ⅲ组Ⅱ组Ⅰ组Ⅳ组;纯利润为Ⅲ组Ⅱ组Ⅳ组Ⅰ组。结果提示,试验Ⅲ组的饲料组成在成本控制和提质增效之间达到最佳平衡,选用蛋白水平28.17%的饲料组成补饲羔羊经济效益较高,可作为羔羊补饲环节的首选配方。  相似文献   

4.
试验旨在探讨土豆渣与劣质牧草混合发酵饲料饲喂对小尾寒羊氨基酸及脂肪酸组成的影响。选择150只健康无病、体重相近(22 kg左右)的小尾寒羊羔羊,随机分成3组,每组5个重复,每个重复10只。对照组常规饲喂(精料+羊草),试验Ⅰ组和试验Ⅱ组精料基础上饲喂不同配方的土豆渣发酵饲料。3组精料饲喂量均为500 g/d,粗饲料自由采食。预试期7 d,试验期65 d。饲喂试验结束后进行屠宰采取背最长肌和肾周脂肪用于氨基酸和脂肪酸分析。结果表明:试验Ⅰ组相比对照组显著提高了缬氨酸、异亮氨酸、亮氨酸和成人必需氨基酸的含量(P0.05),试验Ⅰ组和试验Ⅱ组相比对照组显著提高了苯丙氨酸和婴儿必需氨基酸的含量(P0.05);试验Ⅰ组肌肉脂肪酸中二十一碳烷酸显著高于对照组(P0.05),试验Ⅰ组肾周脂肪酸中硬脂酸显著低于对照组(P0.05)。土豆渣与劣质牧草混合发酵饲料饲喂小尾寒羊可提高肌肉中氨基酸的含量,羊肉营养丰富。脂肪酸而言,饲喂发酵饲料并未改变肌肉和肾周脂肪中的脂肪酸组成,试验组间比较,试验Ⅰ组略优于试验Ⅱ组。  相似文献   

5.
为研究不同蛋白水平饲粮对牦牛肉品质以及饲养效益影响,选取24头健康状况良好,年龄接近,初始体重(200±20)kg的健康公牦牛,随机分为Ⅰ、Ⅱ组,每组各12头。Ⅰ组(低蛋白组)饲粮蛋白质水平为12%,Ⅱ组(高蛋白组)饲粮蛋白质水平为16%,两组饲粮代谢能水平均为11.7 MJ/kg。试验预试期10 d,正试期180 d,记录试验期间牦牛增重和饲喂耗料量,以计算养殖收益。试验结束后每组随机选取5头公牦牛,进行屠宰。屠宰后进行肉品质测定。结果表明:(1)不同蛋白水平饲粮对牦牛背最长肌pH45 min影响显著(P <0.05),均在正常范围。不同蛋白水平饲粮对于公牦牛背最长肌pH24 h、肱三头肌pH45 min和pH24 h影响均不显著(P> 0.05),不同蛋白水平饲粮对于公牦牛肉色亮度、黄度、红度、剪切力和蒸煮损失均影响不显著(P> 0.05)。(2)不同蛋白水平饲粮对于公牦牛肉硬度影响显著(P <0.05),对黏性、弹力、内聚性、可恢复形变、可恢复功、弹性、胶着性均影响不显著(P> 0.05)。(3)相较于高蛋白组,低蛋白组养殖毛利润更高,毛利润提升约8...  相似文献   

6.
本试验旨在探究不同能量和蛋白质水平饲粮交替饲喂对妊娠母猪繁殖性能、血浆激素含量及抗氧化指标的影响。试验分为2个阶段,配制6种试验饲粮,妊娠前期试验饲粮:低能量低蛋白质水平饲粮(12.54 MJ/kg、15.55%)、中能量中蛋白质水平饲粮(13.27 MJ/kg、16.47%)、高能量高蛋白质水平饲粮(13.44 MJ/kg、17.86%);妊娠后期试验饲粮:低能量低蛋白质水平饲粮(12.73 MJ/kg、15.96%)、中能量中蛋白质水平饲粮(13.27 MJ/kg、16.47%)、高能量高蛋白质水平饲粮(13.84 MJ/kg、18.13%)。选取2胎、体重相近的加系大白母猪48头,随机分为对照组(早上饲喂中能量中蛋白质水平饲粮,下午饲喂中能量中蛋白质水平饲粮)、试验Ⅰ组(早上饲喂低能量低蛋白质水平饲粮,下午饲喂高能量高蛋白质水平饲粮)、试验Ⅱ组(早上饲喂高能量高蛋白质水平饲粮,下午饲喂低能量低蛋白质水平饲粮),每组16个重复,每个重复1头母猪。每天饲喂2次。妊娠前期中能量中蛋白质水平饲粮每次饲喂1.125 kg,低能量低蛋白质水平饲粮每次饲喂1.150 kg,高能量高蛋白质水平饲粮每次饲喂1.140 kg;妊娠后期中能量中蛋白质水平饲粮每次饲喂1.805 kg,低能量低蛋白质水平饲粮每次饲喂1.800 kg,高能量高蛋白质水平饲粮每次饲喂1.800 kg。试验期114 d。结果表明:1)与对照组相比,试验Ⅰ组妊娠36~90 d的平均日增重(ADG)显著增加(P<0.05);试验Ⅰ组总产仔数高于对照组(P>0.05);试验Ⅰ组活仔数高于对照组(P>0.05)。2)与对照组相比,试验Ⅰ组妊娠35 d的血浆过氧化氢酶(CAT)活性显著升高(P<0.05),试验Ⅰ组妊娠80 d的血浆CAT活性极显著升高(P<0.01),且试验Ⅰ组较试验Ⅱ组显著升高(P<0.05);与对照组相比,试验Ⅱ组妊娠80 d的血浆丙二醛(MDA)含量极显著降低(P<0.01),试验Ⅰ组妊娠35 d的血浆MDA含量显著高于试验Ⅱ组(P<0.05)。3)与对照组相比,试验Ⅰ组妊娠80 d的血浆瘦素(LEP)含量显著升高(P<0.05);试验组妊娠105 d的血浆胰岛素样生长因子-Ⅰ(IGF-Ⅰ)含量极显著低于对照组(P<0.01)。综上所述,与早、晚饲喂相同营养水平饲粮相比,早、晚交替饲喂不同能量和蛋白质水平饲粮可提高妊娠母猪的繁殖性能,影响血浆中激素含量和抗氧化指标。  相似文献   

7.
该试验比较了哺乳期羔羊补饲三种颗粒料的饲喂效果,结果表明B公司的羔羊补饲颗粒料效果最好。饲喂效果主要取决于颗粒料的粗蛋白含量及赖氨酸含量,以及其转化效率。  相似文献   

8.
试验旨在研究营养限制期不同能氮水平和补偿期对羔羊肉品质的影响。选用80只蒙古羔羊,随机分为4组,分别为对照组(CG)、中等限制Ⅰ组(RG1)、中等限制Ⅱ组(RG2)和严重限制组(RG3)。营养限制期(60 d)4组羔羊日粮能量(ME)和蛋白质(CP)水平分别为10.88、10.88、9.41、8.62 MJ/kg和15.0%、10.0%、10.0%、5.7%。营养补偿期(90 d)4组羔羊日粮能量水平一致(ME 9.75 MJ/kg、CP 12%)。营养限制期结束和补偿期结束时,每组分别屠宰4只羔羊,测定背最长肌pH、失水率和剪切力值及胴体营养水平。结果表明,限制期结束时,RG3组羔羊背最长肌pH1和胴体粗蛋白质、粗灰分含量显著高于CG组(P<0.05),背最长肌剪切力值显著低于CG组(P<0.05),胴体干物质含量极显著低于CG组(P<0.01);补偿期结束时,RG2组羔羊背最长肌pH1显著高于CG组(P<0.05);RG1和RG2组羔羊背最长肌剪切力值和胴体粗蛋白质含量显著高于CG组(P<0.05);RG3组羔羊胴体粗灰分含量显著高于CG组(P<0.05)。因此,背最长肌pH、嫩度及胴体化学组成均受到营养限制的影响,经补偿后除嫩度外均能恢复到对照组水平,且随着受限程度的加剧,恢复速度越慢。  相似文献   

9.
[目的] 研究早期断奶对藏西北绒山羊背最长肌中氨基酸和脂肪酸含量的影响。[方法] 将18只健康羔羊按照体重相近原则随机分为试验组和对照组,试验组在28日龄(D28)实施早期断奶,对照组在60日龄(D60)自然断奶,羔羊断奶后饲喂相同的代乳料至75日龄;从两组随机选取5只羔羊进行屠宰采样,对其背最长肌中氨基酸和脂肪酸指标进行测定分析。[结果] 在藏西北绒山羊背最长肌中检测出17种氨基酸,包括赖氨酸、亮氨酸等7种必需氨基酸,天冬氨酸、精氨酸等10种非必需氨基酸。两组断奶羔羊肉中总氨基酸含量、必需氨基酸含量、非必需氨基酸含量均无显著差异(P>0.05),28日龄断奶组羔羊肉中EAA/TAA、EAA/NEAA比值显著(P<0.05)高于60日龄断奶羔羊。共检测出29种饱和脂肪酸、不饱和脂肪酸,以棕榈酸和油酸含量最高,早期断奶对羔羊肉中饱和脂肪酸含量、单不饱和脂肪酸含量以及多不饱和脂肪酸含量影响不显著(P>0.05)。[结论] 藏西北绒山羊羔羊肉中所含氨基酸和脂肪酸种类多、含量丰富,实施早期断奶、及早补充颗粒料对肉中脂肪酸和氨基酸含量无影响,且可提高EAA/TAA、EAA/NEAA比值,使其更符合FAO/WHO氨基酸评分模式;该试验可为藏西北绒山羊羔羊实施早期断奶、提高母羊的繁殖率提供参考。  相似文献   

10.
为了研究日粮中添加复合益生菌制剂对努比亚山羊肌肉营养成分的影响,试验将36只2月龄左右、健康且体重[(17.06±0.65) kg]相近的努比亚山羊随机分为3组,分别为试验A组(饲喂基础日粮)、试验B组(饲喂基础日粮+0.5‰复合益生菌制剂)、试验C组(饲喂基础日粮+1.0‰复合益生菌制剂),每组12只。各组山羊每天在07:00和18:00各饲喂1次,试验期为70 d。在饲养试验结束当天09:00,每组随机选取4只体重相近的羊只称重后进行屠宰,取背最长肌,检测水分、粗蛋白、粗脂肪、氨基酸及脂肪酸含量。结果表明:试验C组山羊背最长肌中水分含量显著低于试验A组(P<0.05);试验B、C组粗蛋白和粗脂肪含量均低于试验A组,但差异不显著(P>0.05)。山羊背最长肌中检测到7种必需氨基酸和9种非必需氨基酸,其中试验B、C组组氨酸含量高于试验A组,其余氨基酸(除赖氨酸、天冬氨酸外)含量均低于试验A组,均差异不显著(P>0.05)。山羊背最长肌中检测到14种脂肪酸,其中除试验B组的二十碳三烯酸(C20∶3n6)含量显著低于试验A组(P<0.05)外,其余各组间脂肪酸含量均...  相似文献   

11.
Effects of considering the comminution rate (kc) and the correction of microbial contamination (using 15N techniques) of particles in the rumen on estimates of ruminally undegraded fractions and their intestinal digestibility were examined generating composite samples (from rumen‐incubated residues) representative of the undegraded feed rumen outflow. The study used sunflower meal (SFM) and Italian ryegrass hay (RGH) and three rumen and duodenum cannulated wethers fed with a 40:60 RGH to concentrate diet (75 g DM/kgBW0.75). Transit studies up to the duodenum with Yb‐SFM and Eu‐RGH marked samples showed higher kc values (/h) in SFM than in RGH (0.577 vs. 0.0892, p = 0.034), whereas similar values occurred for the rumen passage rate (kp). Estimates of ruminally undegraded and intestinal digestibility of all tested fractions decreased when kc was considered and also applying microbial correction. Thus, microbial uncorrected kp‐based proportions of intestinal digested undegraded crude protein overestimated those corrected and kc?kp‐based by 39% in SFM (0.146 vs. 0.105) and 761% in RGH (0.373 vs. 0.0433). Results show that both kc and microbial contamination correction should be considered to obtain accurate in situ estimates in grasses, whereas in protein concentrates not considering kc is an important source of error.  相似文献   

12.
Capromorelin is a ghrelin receptor agonist that is FDA approved for appetite stimulation in dogs. The objective of this study was to evaluate the safety of daily oral administration of capromorelin to cats over a range of doses and for an extended period. Two randomized, controlled studies were conducted: in Study 1, cats (= 6 per group) received placebo or capromorelin at a dose of 9, 15, 30 or 60 mg/kg once daily for 14 days; and in Study 2, cats received capromorelin at 6 mg/kg (= 8) or placebo (= 4) once daily for 91 days. Cats were evaluated using clinical observations and clinical pathology test results for both studies, with the addition of postmortem examination in Study 1 and measurements of growth hormone and insulin‐like growth factor 1 in Study 2. Abnormal clinical observations were limited to emesis, hypersalivation, lethargy/depression, head shaking and lip smacking, which occurred more frequently in the capromorelin‐treated groups than in the placebo group. There were no clinically relevant differences in clinical pathology test results between the capromorelin and placebo groups in either study.  相似文献   

13.
Activation of polymorphonuclear cells (PMNs) leads to the formation of superoxide, which is in turn dismutated to H2O2 by superoxide dismutase (SOD) and is partly responsible for oxygen-dependent microbicidal activity. However, no comparative information is available on the effect of SOD inhibition before PMN activation to allow simulation of the SOD defects that are known to occur in some ruminants. This paper attempts to examine the degranulative and phagocytic responses in buffalo, cattle and goat PMNs exposed to diethyldithiocarbamate, a known SOD inhibitor. The activity of glutathione peroxidase and reductase was increased in the presence of SOD inhibitor. On activation, H2O2 production increased significantly (p<0.01), while SOD inhibition before the activation of PMNs caused a significant decline in the production of H2O2 (p<0.05) in all the species studied. There was a significant increase (p<0.05) in the phagocytosis of Candida albicans spores by buffalo PMNs activated with opsonized zymosan. Activation of bovine PMNs after exposure to the SOD inhibitor resulted in a significant decline (p<0.05) in phagocytic activity; in the other species, the two values only approached significance. Among the activators, opsonized zymosan caused a significant increase in phagocytic activity as compared to lipopolysaccharide, particularly in the PMNs of buffaloes (p<0.05). Increased fungicidal activity (p<0.05) occurred with opsonized zymosan-activated PMNs of all the species studied. The fungicidal activity was found to decline in PMNs exposed to SOD inhibitor before activation (p<0.05). Interestingly, the phagocytic activity of caprine PMNs was found to be lower than that of PMNs from cattle (p<0.05).  相似文献   

14.
分别利用筛选出的多态性高、谱带清晰的8条ISSR标记和18对SRAP标记对海南岛69份红毛丹种质资源进行PCR扩增,8条ISSR引物共检测到425个多态性条带,其中品种(系)特异条带47个,平均位点多态性信息含量(PIC)值为0.928,可鉴别的种质资源数25~67份;18对SRAP引物共检测到894个多态性条带,其中品种(系)特异条带69个,平均位点多态性信息含量(PIC)值为0.936,可区分的种质资源数为17~63份。综合各项指标利用ISSR16和ISSR5引物21个条带用于构建红毛丹种质DNA指纹图谱,该图谱可有效区分69份红毛丹种质资源。同时该DNA指纹图谱还可为下一步红毛丹种质资源鉴定、利用、品种权保护和分子育种等提供了理论依据。  相似文献   

15.
This study was conducted to investigate the effect of different dietary ratios of 13 C to 12 C or 15 N to 14 N on their relative incorporation into tissues. Eighty male rats were used in two 21-day feeding trials in which they were fed diets with either high δ13C levels (δ13C = −13.89‰ and δ15N = 2.37‰ in experiment 1 and δ13C = −19.34‰ and δ15N = 4.73‰ in experiment 2) or low δ13C levels (δ13C = −17.90‰ and δ15N = 3.08‰ in experiment 1 and δ13C = −21.76‰ and δ15N = 0.53‰ in experiment 2), meanwhile, the dietary δ15N levels were designed to two ranks. Blood, liver, adipose and muscle tissues were collected on day 0, 3, 7, 14, and 21 for determination of 13 C, 12 C, 15 N and 14 N isotopes. Rat growth rate, antioxidant capacity and metabolic parameters were also assessed. The results indicate that adipose tissue tend to deplete 13 C before the stable isotopic ratios achieved final equilibrium. Therefore, feeds with different isotopic signatures had different incorporation rates into tissues. Low dietary 13 C levels decreased tissue δ13C values whereas high dietary 13 C levels did not alter tissue δ13C values during the 21-d experiment. Blood δ15N values were a reliable parameter in assessing the relative contribution of dietary nitrogen to tissues. This study revealed a relationship between dietary isotopic signatures and their incorporation rates into rat tissues. However, more studies are needed to illustrate the mechanism through which dietary isotopic ratios influence the extent of isotopic incorporation into the tissues.  相似文献   

16.
对10个青贮玉米品种自然发病情况进行调查,并采用病级分类方法对抗病性分类。结果表明:对锈病表现抗性和中抗的品种有3个和5个,分别占供试品种的30%和50%;对大斑病表现抗性和中抗的品种有4个和5个,分别占供试品种的40%和50%;对褐斑病表现抗性和中抗的品种有6个和3个,分别占供试品种的60%和30%;对小斑病表现抗性和中抗的品种有6个和3个,分别占供试品种的60%和30%。多数品种对青贮玉米4种病害抗性表现较好,可从青贮玉米供试材料中选用优良抗病材料用于四川地区种植。  相似文献   

17.
The pharmacokinetic properties of three formulations of vitacoxib were investigated in horses. To describe plasma concentrations and characterize the pharmacokinetics, 6 healthy adult Chinese Mongolian horses were administered a single dose of 0.1 mg/kg bodyweight intravenous (i.v.), oral paste, or oral tablet vitacoxib in a 3-way, randomized, parallel design. Blood samples were collected prior to and at various times up to 72 hr postadministration. Plasma vitacoxib concentrations were quantified using UPLC-MS/MS, and pharmacokinetic parameters were calculated using noncompartmental analysis. No complications resulting from the vitacoxib administration were noted on subsequent administrations, and all procedures were tolerated well by the horses throughout the study. The elimination half-life (T1/2λz) was 4.24 ± 1.98 hr (i.v.), 8.77 ± 0.91 hr (oral paste), and 8.12 ± 4.24 hr (oral tablet), respectively. Maximum plasma concentration (Cmax) was 28.61 ± 9.29 ng/ml (oral paste) and 19.64 ± 9.26 ng/ml (oral tablet), respectively. Area under the concentration-versus-time curve (AUClast) was 336 ± 229 ng hr/ml (i.v.), 221 ± 94 ng hr/ml (oral paste), and 203 ± 139 ng hr/ml, respectively. The results showed statistically significant differences between the 2 oral vitacoxib groups in Tmax value. T1/2λz (hr), AUClast (ng hr/ml), and MRT (hr) were significantly different between i.v. and oral groups. The longer half-life observed following oral administration was consistent with the flip-flop phenomenon.  相似文献   

18.
本文以千日红组培苗为试验材料,研究培养基中氮形态及含量对千日红试管苗生长和开花诱导的影响。结果表明,1)相对于铵态氮(NH4+),硝态氮(No3-)作为唯一氮源更有利于千日红试管苗生长和开花诱导,但千日红在NH4+和NO3-同时存在的培养基中表现最佳。2)在20 mmol/LNH4+(NO3-)和5 mg/L PP333存在的条件下,试管苗生长基本随着培养基中NO3-(NH4+)含量的增加而增加,并在含40 mmol/L NO3-+20 mmol/L NH4+(即MS培养基中氮含量)的培养基中株高达到最大值5.91 cm;而叶片数和开花率则随着培养基中NH4+和NO3-含量的增加呈现先增加后下降的趋势,并在20 mmol/L NO3-+5 mmol/LNH4+培养基中达到最大值,分别为10.7片/株和38.89%。3)氮含量及形态配比结果表明,千日红试管苗开花率在培养基中氮总量为5 mmol/L、NO3-/NH4+为4/1时达到最大值39.95%,而株高和叶片数在氮总量为35 mmol/L,NO3-/NH4+为4/1时达到最大值8.52 cm和13.38片/株。千日红试管苗开花率与培养基中NO3-/NH4+显著正相关,而与氮总量及株高之间显著负相关。此外,培养基中氮含量及形态配比还显著影响无菌苗根系生长。  相似文献   

19.
Difficulties in applying in vivo methods encourage the prediction of energy values of feeds in horses from alternative methods. Previous attempts to predict energy values from chemical composition have ignored differences among experiments. The current evaluation aimed to find the most reliable predictors (chemical composition and nutrient digestibilities) of apparent total tract energy digestibility of hays. Prediction was done with treatment means from a diverse collection of studies conducted worldwide. Experiment was included as a random effect in models to control for differences among experiments, and treatments means were weighted to account for unequal variances of the means across experiments. Organic matter digestibility (OMD; %) proved to be the most reliable (root mean square error [RMSE] = 1.6486; R2 = 0.8871) predictor of gross energy digestibility (GED; %) when calculated from 44 treatment means (n) obtained from 28 experiments conducted in 16 different studies (GED = 5.527 + 0.8124 OMD). Neutral detergent fiber (NDF; g/kg dry matter) presented a single chemical entity that was preferred over any multipredictor equation (GED = 70.4638−0.0360 NDF; RMSE = 2.9462; R2 = 0.6309; n = 58). However, the obtained equations should be validated against observed values, whereas alternative methods should be explored to predict OMD.  相似文献   

20.

Objective

To test the postexposure analgesic efficacy of low doses of eugenol in zebrafish.

Study design

Prospective experimental study.

Animals

A total of 76 large adult zebrafish (Danio rerio).

Methods

Fish swimming behavior (median velocity, freeze time, high-speed swimming and distance moved in the vertical direction) was recorded in a 1.6 L video arena before and after exposure to eugenol (0, 1, 2, 5, 10 and 20 mg L?1). In a second experiment, fish were anesthetized with 2-phenoxy-ethanol and treated with an injection of 5% acetic acid (noxious stimulus), and then exposed to 0, 1, 2 and 5 mg L?1 eugenol. The fish swimming behavior was also recorded.

Results

The higher doses (10 and 20 mg L?1) reduced the median velocity, high-speed swimming and distance moved in the vertical direction, and increased the freeze time. Zebrafish behavior was not altered by eugenol (1, 2 and 5 mg L?1) after noxious stimulation.

Conclusions and clinical relevance

The change in the behavior of zebrafish associated with a noxious stimulus can be monitored and is a good model for studying analgesia in fish. Eugenol (10 and 20 mg L?1) induced zebrafish sedation. The response after a noxious stimulus was not affected by postexposure to lower doses, and thus we cannot recommend its use as an analgesic.  相似文献   

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