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1.
海水养殖杂交鲟对环境变化耐受性的试验研究   总被引:1,自引:0,他引:1  
试验结果表明:海水养殖杂交鲟的适宜温度为5.0~22.0℃,临界上限水温为26.0℃,临界下限水温为3.0℃;耐受盐度的范围比较广泛,为1.0~38.0,适宜养殖的盐度为8.0~32.0;耐pH值的范围为4.7~9.8,适宜养殖的pH值为6.9~8.8。杂交鲟对环境变化耐受性范围较大,可以保证杂交鲟可以更好地适应其生存环境,以避免由于环境因子的激变造成海水养殖杂交鲟的大规模死亡。  相似文献   

2.
为研究珍珠龙胆石斑鱼对海水温度、盐度、溶解氧等主要环境因子的耐受,选择健康活泼的珍珠龙胆石斑鱼个体,采用渐变式方法达到设计的要求,进行试验。结果表明:珍珠龙胆石斑鱼对水温、盐度和溶解氧耐受范围较广,适宜水温为20~35℃,临界下限水温为10℃,临界上限水温为39℃;适宜盐度为11~40,临界下限盐度为3,上限盐度可到45以上;耐受低氧:DO_1为0.70 mg/L,DO_(50)为0.67 mg/L,DO_(100)为0.60 mg/L。  相似文献   

3.
大黄鱼幼鱼对若干环境因子的适应性试验   总被引:1,自引:0,他引:1       下载免费PDF全文
在有实验装置的人工控制环境条件下,以每批100尾大黄鱼幼鱼进行光照强度、水温、溶氧量、盐度等的单因子适应性实验以及对温度、溶解氧和盐度正交适应性实验。其结果是:全长29~44mm,平均全长为39mm的幼鱼.适光范围为520~5500lx,最适范围为780—1830lx;适宜温度为21.4~24.9℃,最适温度为22.4~24.9℃;适宜盐度为25.5~28.8,最适盐度为26.8~28.8;适宜溶氧量为4.70~5.38mg/L,最适溶氧量为4.76~5.15mg/L。全长为59~86mn,平均全长为75mm的幼鱼,适光范围为620~5500lx,最适光照强度为850~1820lx;适宜温度为22.3~25.0℃,最适温度为22.3~24.3℃;适宜盐度为26.5~28.5,最适盐度为26.7~27.8;适宜溶氧量为4.65~5.38mg/L,最适溶氧量为4.75~5.21mg/L。温度对幼鱼的影响极为显著,盐度和溶解氧的影响次之。  相似文献   

4.
为研究黄斑蓝子鱼(Siganus oramin)幼鱼对养殖环境的耐受力,对体质量为(3.25±0.83) g的黄斑蓝子鱼幼鱼开展了水温、盐度和溶解氧等环境因子的耐受性实验,同时监测了极限水温和盐度条件下黄斑蓝子鱼幼鱼的耗氧率和呼吸频率变化,以期为黄斑蓝子鱼的养殖生产提供参考。结果显示,黄斑蓝子鱼幼鱼适宜水温为23.0~28.0℃,可耐受的水温范围为12.1~32.5℃;适宜盐度为8.0‰~59.0‰,可耐受的盐度范围为3.5‰~75.0‰;正常环境下窒息临界点溶解氧质量浓度为(1.47±0.52) mg·L-1。临近耐受极限的水温和盐度均对黄斑蓝子鱼幼鱼的窒息临界点、窒息点以及极限低氧耐受有较大影响,其中低盐对窒息点的影响最大。研究表明,黄斑蓝子鱼幼鱼对温度和盐度耐受范围较广,但超出适宜范围时耗氧率和呼吸频率均发生显著性变化,出现应激反应;黄斑蓝子鱼幼鱼窒息点高,易缺氧,建议养殖全过程溶解氧质量浓度不低于6 mg·L-1。  相似文献   

5.
莫桑比克鳗鲡对温度、盐度和pH的耐受性试验   总被引:2,自引:0,他引:2  
就莫桑比克鳗鲡对水温、盐度和pH的耐受性进行了初步研究.试验结果表明,适宜水温为12.0~35.0℃,临界上限为40.0℃.临界下限为0℃;适宜盐度为0~15.临界极限为45;适宜pH为4.0~9.5,临界上限为11.0,临界下限为2.5.  相似文献   

6.
单环刺螠增养殖生物学的研究   总被引:3,自引:0,他引:3  
单环刺螠个体鲜出肉率大致为冬季39~47%,春季17~27%。食性为滤食性,以水中悬浮性颗粒为食。气温7℃,干露时间持续72h.均能成活。生活水温是0~30℃,临界高温32.5℃,临界低温-4℃。生活的盐度范围是15~36,适宜盐度范围24.94~35.77。pH值的耐受范围是4.46~9.5,适宜范围7.5~9.42。在溶解氧含量达1.14mg/L时,活动正常。  相似文献   

7.
光裸方格星虫初期海球幼体对温度和盐度的耐受试验   总被引:1,自引:1,他引:0  
在实验室条件下开展光裸方格星虫初期海球幼体对温度和盐度的耐受实验。结果表明,在盐度32,试验时间24h内,光裸方格星虫幼体的正常发育生长温度范围为22~38℃,临界上限水温为39℃,临界下限水温为1℃。耐受的盐度范围较广,在试验时间48h内,温度26~27℃条件下,盐度17~47范围内幼体都可存活,其中盐度24~36试验组幼体发育正常,在中上水层游动,具趋光性和聚集性,生长速度较其它试验组快;临界上限盐度为50,临界下限盐度为14。这些表明光裸方格星虫初期海球幼体对低盐度相对敏感,对高盐度有较强的耐受力,而对温度特别是低温的耐受能力也较强。  相似文献   

8.
理化因子对原绿球藻生长及其色素含量的影响   总被引:4,自引:0,他引:4  
冯宪栋  蒋霞敏  符方尧 《水产科学》2007,26(12):643-647
以COA为基础培养基,进行了光照、温度、盐度等理化因子对原绿球藻生长和色素含量影响的单因子试验。试验结果表明,原绿球藻适宜生长的光照度为500~2500lx,最适光照度为500~1000lx:适宜生长的温度为10~30℃,最适温度为25~30℃;适宜生长的盐度为0~44.9,最适盐度为13.7~33.4;适宜生长的pH5~11,最适pH8~10;最适氮源为NH4-N,其次为(NH2)2CO;适宜生长的氮(NH4Cl)质量浓度为5~25mg/L,最适质量浓度为15~25mg/L。理化因子对该藻色素含量的影响与其生长相吻合。  相似文献   

9.
莫桑比克鳗鲡养殖生物学主要特性研究   总被引:5,自引:1,他引:4  
对莫桑比克鳗鲡(Anguillamor morata)玻璃鳗经形态学鉴定品种后,开展了主要养殖生物学特性研究。耐受高温和低温分别为39℃和7℃,适宜生存水温15℃~33℃;耐受盐度与水温相关,水温30℃致死盐度2.9%,适宜盐度小于1.1%,20℃致死盐度2.7%,适宜盐度小于1.8%,10℃致死盐度2.1%,适宜盐度小于2.0%;耐受pH值与水温相关,30℃致死pH值分别为3.5和11.0,适宜pH值6.0—7.0,20℃致死pH值分别为4.0和11.0,适宜pH值6.0~8.0,10℃致死pH值分别为3.5和10.5,适宜pH值5.5—8.5。常用药物S高聚碘、二氧化氯、三氯异氰尿酸、苯扎溴铵、溴氯海因、甲醛、敌百虫的安全浓度高于或接近常用浓度,硫酸铜、溴氰菊脂乳油、氯氰菊脂、甲苯咪唑的安全浓度低于常用浓度。水体中非离子态氨的安全浓度为2.51mg/L,亚硝酸氮的安全浓度为55.12mg/L。  相似文献   

10.
长蛸对几个环境因子的耐受能力及栖息习性研究   总被引:3,自引:0,他引:3  
研究了长蛸成体的栖息习性及对水体环境的盐度、温度、pH的耐受能力。通过设置红砖搭穴、瓦片搭穴、石块搭穴和白塑料管作为长蛸的隐蔽场所,使用控温电热棒和冰袋调温并保持水温,用NaOH和HCl调节水体的pH值,加食盐或淡水调配水体的盐度。试验结果表明,长蛸喜栖于砖、瓦、石块下,以瓦片为最佳隐蔽场所;喜欢泥质底,有钻泥打洞现象;适宜盐度为16.3~27.3,最适盐度为18.3~24.3;适宜温度为10~31℃,最适温度为12~27℃;适宜pH为6.2~9.7,最适为6.7~9.2。  相似文献   

11.
The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.  相似文献   

12.
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China.  相似文献   

13.
14.
In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.  相似文献   

15.
Growth hormone (GH) and reproduction: a review   总被引:5,自引:0,他引:5  
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations.  相似文献   

16.
Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation.  相似文献   

17.
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality).  相似文献   

18.
Protein and amino acid composition of the mantle of juvenile O ctopus vulgaris (Cuvier, 1797) during fasting for 27 days were determined. Average protein content of octopus mantle was of 711.19 ± 46.80 g kg?1 DW, and it decreased with increasing fasting days. The non‐essential amino acids content was higher (486.18 ± 11.08 g kg?1 protein) than essential amino acids (425.82 ± 9.15 g kg?1 protein) at the start of the experiment (unstarved animals). The results suggest that the amino acid profile of the mantle where the most abundant amino acids are Arg, His, Lys, Gly, Leu and Pro could indicate a prolonged fasting condition (>20 days) or poor nutrition of O . vulgaris. This study supports the idea of using mantle for metabolic needs of starved O . vulgaris suggesting that the degradation pathway of amino acids to pyruvate and tricarboxylic acid cycle intermediates was favoured contrary to the degradation pathway of ketogenic amino acids. Special considerations should be taken concerning Thr, Ile, Ser, Ala, Asx (Asp, Asn), Glx (Glu, Gln) (because of their fast intake) and Lys and His (due to their stable contents) during a prolonged period of fasting.  相似文献   

19.
The endemic, anadromous cyprinidChalcalburnus tarichi is the only fish species known to occur in alkaline Lake Van (Eastern Anatolia, Turkey). EightC. tarichi were maintained individually in Lake Van water (17 – 19°C; pH 9.8; 153 mEq·I–1 total alkalinity; 22 total salinity) and tank water samples analyzed for 24 h in 2 to 4 h intervals. At zero time, < 1µM ammonia was present and urea was undetectable in the tank water; at 24 h, total ammonia and urea made up 114±32 and 35±25µM, respectively. Over the experimental period, ammonia-N and urea-N excretion averaged 1041±494 and 607±169moles·kg–1 fish·h–1, respectively. The extent of urea excretion was highly variable between specimens. Uric acid excretion was not detectable.Urea was present at high concentrations in all tissues and plasma (25 – 35moles·g–1·ml–1) of freshly caughtC. tarichi; total ammonia content of the tissues was by a factor of 1.9 (liver) to 3.0 (brain) lower. High arginase activity (2.4±0.2 U·min–1·g–1) was detected in the liver ofC. tarichi but ornithine carbamoylphosphate transferase, a key enzyme of the ornithine-urea-cycle, was absent. Ureagenesis is likely through degradation of arginine and/or uricolysis. High glutamine synthetase activity (11±0.6 U·min–1·g–1) and low ammonia content in brain suggest that, like other teleosts,C. tarichi has an efficient ammonia detoxification in the brain, but in no other tissue.Nitrogenous waste excretion at alkaline pH is discussed. The ability ofC. tarichi to excrete high levels of ammonia at extremely alkaline pH is unique among teleosts studied so far. The mechanism of ammonia excretion under Lake Van conditions remains to be elucidated.  相似文献   

20.
There has been growing concern about the overuse of antibiotics in the ornamental fish industry and its possible effect on the increasing drug resistance in both commensal and pathogenic organisms in these fish. The aim of this study was to carry out an assessment of the diversity of bacteria, including pathogens, in ornamental fish species imported into North America and to assess their antibiotic resistance. Kidney samples were collected from 32 freshwater ornamental fish of various species, which arrived to an importing facility in Portland, Oregon from Colombia, Singapore and Florida. Sixty‐four unique bacterial colonies were isolated and identified by PCR using bacterial 16S primers and DNA sequencing. Multiple isolates were identified as bacteria with potential to cause disease in both fish and humans. The antibiotic resistance profile of each isolate was performed for nine different antibiotics. Among them, cefotaxime (16% resistance among isolates) was the antibiotic associated with more activity, while the least active was tetracycline (77% resistant). Knowing information about the diversity of bacteria in imported ornamental fish, as well as the resistance profiles for the bacteria will be useful in more effectively treating clinical infected fish, and also potential zoonoses in the future.  相似文献   

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