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采用PCR产物直接测序法分别测定了条斑星鲽和圆斑星鲽线粒体基因组序列,并对12SrRNA和16S rRNA基因核苷酸全序列进行分析,条斑星鲽和圆斑星鲽线粒体12S rRNA的核苷酸序列长度分别为948 bp和949 bp,16S rRNA均为1716 bp。试验结果表明,由12S rRNA和16S rRNA基因所构建的两个系统树的结构基本一致,21种鱼主要分为3个大的分支:鲤形目、鲶形目聚为1支,鲑形目独立聚为1支,鲈形目和鲽形目聚为1大支。鲆鲽类与鲈形目的鲹科、鲷科鱼类聚类在一支,且与鲹科的日本竹荚鱼、大西洋竹荚鱼构成姊妹群,支持鲆、鲽类是从鲈形目分化出来的观点,而同属于鲽形目的鳎科鱼类塞内加尔鳎在12S rRNA树中成为一个独立的分支,在16S rRNA树中聚到鲈形目和鲽形目的分支中,并且与鲈形目关系更近些,鳎类是1个特殊类群,其分类地位有待进一步探讨。线粒体基因组序列已提交到GenBank中,序列号为:DQ403797和EF025506。 相似文献
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Characterization of the 26S Proteasome S7 Subunit Gene and Its Expression during Regeneration in Apostichopus japonicus 
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下载免费PDF全文 Yanjie Qin Xiangji Zhao Xia Li Yanfeng Wang Yang Liu 《Journal of the World Aquaculture Society》2015,46(2):159-170
The 26S proteasome, which includes 20S core and 19S regulatory particles, plays an important role in removing harmful and/or unnecessary proteins. In this study, we cloned a 2443‐bp full‐length cDNA sequence of the S7 subunit of 19S from the sea cucumber Apostichopus japonicus, including a 70‐bp 5′‐untranslated region (UTR) and 1070‐bp 3′‐UTR coding for a theoretical 435 amino acid protein. Sequence analysis showed that S7 belongs to the ATPases associated with a wide variety of cellular activities (AAA+) superfamily. S7 was widely expressed in different tissues as determined by real‐time quantitative polymerase chain reaction, and its expression was gradually upregulated in the regenerating body wall. Combined with histological observations, our results suggest that the S7 subunit or the 26S proteasome assembled based on S7 may be associated with the regulation of cell migration and cell cycle during body wall regeneration in A. japonicus. 相似文献
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用两种营养强化剂(ESP,S．S)强化轮虫、卤虫活饵料,以探讨这两种强化剂对大菱鲆生长、存活及色素沉积情况。结果发现ESP组能明显改善大菱鲆的色素异常,两组大菱鲆生长相似。用ESP强化组的存活率高于S．S强化组。 相似文献
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Carole R. Engle Nathan M. Stone 《Aquaculture Economics & Management (Blackwell Science)》2013,17(3):251-280
Increased attention has been paid in recent years to both positive and negative effects of increasing numbers of regulations on businesses in the United States. The decline in U.S. aquaculture has been attributed in part to increasing volumes of imports and high feed prices. However, there is increasing concern that the U.S. regulatory environment, as compared to that of international competitors, may also have contributed to this decline. More than 1,300 laws apply to U.S. aquaculture and even though the majority has been issued by individual states and apply only to specific types of aquaculture businesses in that state, the cumulative regulatory burden has increased over time. Major compliance categories include: 1) environmental management; 2) food safety; 3) legal and labor standards; 4) interstate transport of aquatic products; 5) fish health; and 6) culture of commercially harvested species. A substantial portion of the regulatory burden is the managerial and labor time spent on compliance in addition to the direct cost increases. The streamlined one-stop process adopted in Norway appears to have allowed growth of aquaculture within a comprehensive regulatory framework, yet the lack of such a streamlined approach in the United States appears to have contributed to the decline of existing industries and to serve as a deterrent to investment in newly emerging technologies. Favorable regulatory environments in countries that export to the U.S. contrast sharply with the increasingly inefficient, cumbersome and/or restrictive U.S. environment. Such disparities have created competitive disadvantages for U.S. producers. Attention is needed by policy makers to search for streamlining mechanisms and by the scientific community to address the growing competitive disadvantage to U.S. aquaculture to respond to increased global demand for farmed seafood. 相似文献
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Gina L. Shamshak James L. Anderson Frank Asche Taryn Garlock David C. Love 《Journal of the World Aquaculture Society》2019,50(4):715-727
U.S. seafood consumption has changed dramatically in recent decades and has become increasingly dominated by the consumption of a limited number of species that are primarily imported and predominantly sourced from aquaculture. In getting to this point, the United States has been, and still is, at the forefront of some of the most important trends in global seafood markets. Hence, discussing the factors influencing U.S. seafood consumption patterns is an interesting and informative endeavor and will most likely also have strong predictive power for the continued development of seafood markets in the United States. In this article, we will discuss the transitions in the U.S. seafood market, primarily focusing on the period from 1990 to the present, highlighting the main factors that facilitated this development. This article provides an overview of U.S. landings, aquaculture production, exports, and imports and also explores contributing trends in global export and import markets. This will be followed by a discussion of U.S. per capita consumption patterns and an examination of the consolidation of species consumed over time. Finally, implications for future trends in seafood consumption and production are discussed. 相似文献
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Henneke Pangkey Keniji Hara Katsuyasu Tachibana Min-Jie Cao Kiyoshi Osatomi and Tadashi Ishihara 《Fisheries Science》2000,66(6):1130-1137
SUMMARY: Cathepsin S was purified from carp hepatopancreas to homogeneity up to 300-fold. The amino acid sequence of its NH2 -terminus was determined to be V-P-D-A-M-D-W-Y-N-K-G-Y-V-T-D-V-K-N-Q. On the contrary, that of purified cathepsin L from carp hepatopancreas was to be V-P-N-S-L-D-W-R-E-K-G. Purified cathepsin S consisted of a single chain with 37 kDa estimated by sodium dodecylsulfate–polyacrylamide gel electrophoresis. The enzyme had strong hydrolytic activity toward Z-Phe-Arg-MCA with the pH optimum of 7.0, but this lacked the ability to hydrolyze most of the other MCA substrates. The optimum pH of cathepsin S for protein substrate (carp myosin heavy chain) was also to be pH 7.0. These properties of purified cathepsin S obviously differ from cathepsins B and L. The enzyme activity was totally inhibited by E-64, leupeptin, 5–5'-dithiobis (2-nitro-benzoic acid) and p -tosyl-lys chloromethylketone as well. 相似文献
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Henneke Pangkey Kenji Hara Katsuyasu Tachibana Min-Jie Cao Kiyoshi Osatomi and Tadashi Ishihara 《Fisheries Science》2000,66(6):1138-1143
SUMMARY: From 250 g of carp hepatopancreas, 0.29 mg of the purified enzyme was obtained. The bond specificity of cathepsin S for α-neoendorphin and neurotensin was 6 Arg-7 Lys and 3 Tyr-4 Glu, respectively. The cleavage sites for insulin B-chain were estimated to be the bonds at 3 Asn-4 Gln, 6 Leu-7 Cys, 12 Val-13 Glu, 13 Glu-14 Ala, 16 Tyr-17 Leu, 22 Arg-23 Gly, 24 Phe-25 Phe and 26 Tyr-27 Thr. P2 position on these peptides were bulky and hydrophobic amino acid residues such as Phe or Leu, small amino acid residues such as Gly, Ala and Val were also accepted in these positions. Regarding the protein substrates, cathepsin S degraded carp α-actinin, actin, tropomyosin and troponins T and I although the proteolyzing speeds were distinct from one another. 相似文献
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根据近源物种线粒体序列的同源比对,在16S rRNA基因上下游保守区域设计一对通用引物。PCR扩增获得特异的DNA片段,经克隆、测序和比对证实该片段包含了卵形鲳鲹线粒体16S rRNA全长序列1725bp。对5个个体分别测序后比对,发现卵形鲳鲹16S rRNA基因在钦州湾种群个体间存在至少7个变异位点,使5个个体分别具有5种不同的单倍型。将卵形鲳鲹和鲹科其它物种的16S rRNA序列进行比,根据比对结果构建的鲳鲹科各物种的系统进化树,支持鲹科下设四个亚科(鲹亚科,鰤亚科,鲳鲹亚科,鰆鲹亚科)的分类系统。综上所述,16S rRNA基因既可用于卵形鲳鲹种群遗传多样性分析,又适用于鲹科鱼类的系统进化分析。 相似文献
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美洲红点鲑和白斑红点鲑及其杂交子代幼鱼的生长曲线拟合 总被引：2，自引：0，他引：2
自2011年11月至2013年1月,对美洲红点鲑(Salvelinus fominalis)和白斑红点鲑(Salvelinus leucomaenis)及其杂交子代从受精卵到1年零两个月幼鱼的生长特性进行了研究。以美洲红点鲑和白斑红点鲑为亲本,建立2×2双列杂交组合,采用Logistic、Gompertz和Von Bertalanffy 3种动物生长模型对美洲红点鲑(A)和白斑红点鲑(B)及其杂交子代进行生长曲线拟合并分析比较其生长性能。实验设置自交组AA、BB和杂交组AB(A♀×B♂)、BA(B♀×A♂)4个实验组。根据模型拟合的R2值判断各组生长性能的拟合程度,并对其体质量、体长、成活率及肥满度进行比较。结果发现,Gompertz生长模型的拟合效果最好,R2均大于0.99,其中AB组生长优势明显。成活率由高到低依次为:AA、AB、BA、BB,但AA组与其他3组的成活率差异不显著(P0.05)。增重率(g/d)和肥满度(g/cm3)等生长指标均由高到低依次为:AB、AA、BA、BB,各组间差异显著(P0.05)。研究结果显示,正交子代(A♀×B♂)幼鱼生长性能明显高于反交子代(B♀×A♂)及其亲本,可见此杂交组合可能具有较高的推广潜力。 相似文献
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10种海参16SrDNA 序列多样性及其亲缘关系分析 总被引：1，自引：1，他引：0
运用PCR扩增10种海参的16SrDNA部分序列,并测序.获得大小为566bp的序列,利用DNAsp4.0软件分析比较10种海参的碱基组成、各碱基变异位点数、插入或缺失位点数,并采用MEGA4.0软件分析彼此间的遗传距离.结果发现,10种海参富含AT碱基,A T的含量平均为57.0%,不同种海参间发生碱基转换、颠换及发生插入或缺失变异的位点数差异很大,10种海参彼此间的遗传距离在0.007～0.316之间.所得结果与GenBank中10种海参的相应序列进行比对分析,同时用MEGA4.0和PAUP4.0软件构建进化树,分析其亲缘关系.结果表明,利用16SrDNA序列的差异可以将分属于海参科(Holothuriidae)与刺参科(Stichopodidae)的海参完全分开,瓜参科(Cucumariidae)的2个种与刺参科的亲缘关系较近.对不同海参种间亲缘关系的分析结果还表明,同属于剌参科的仿刺参(Apostichopus japonicus)与加州拟刺参(Parastichopus californicus)和具疣拟刺参(Parastichopus parvimensis)亲缘关系最近,同属于海参科的格皮氏海参(Pearsonothuria graeffei)与白尼参属的蛇目白尼参(Bohadschia argus)和图纹白尼参(B.marmorata)亲缘关系最近,而同样为海参科的墨西哥海参(Holothuria mexicana)则与红腹海参(Hothuria edulis)关系最近.这些资料为海参的种质资源管理、新种引进和种质改良提供了基础的分子生物学依据. 相似文献