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1.
vMDV感染雏鸡的Se-GSH-PX活性和LPO含量 总被引：2，自引：0，他引：2
以马立克氏病强毒（ｖＭＤＶ）人工感染１日龄ＡＡ肉用雏鸡,于感染后７,１４,２８,４２和５６日龄分别检测雏鸡中枢、外周淋巴器官及主要内脏器官的含硒谷胱甘肽过氧化物酶（Ｓｅ－ＧＳＨ－ＰＸ）活性和脂质过氧化物（ＬＰＯ）含量的变化。结果表明,ｖＭＤＶ感染雏鸡各器官Ｓｅ－ＧＳＨ－ＰＸ活性在检测各时期多显著低于健康对照雏鸡（Ｐ＜０．０５,Ｐ＜０．０１）,而各器官ＬＰＯ含量则多显著高于健康对照雏鸡（Ｐ＜０．０５,Ｐ＜０．０１）,提示马立克氏病（ＭＤ）及其肿瘤的发生和发展与Ｓｅ－ＧＳＨ－ＰＸ抗氧化活性降低和ＬＰＯ及其降解物对雏鸡的广泛病理损伤有关。 相似文献
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山羊内毒素休克诱发脂质过氧化作用及山莨菪碱对其影响的研究 总被引：13，自引：0，他引：13
本文将３０头杂交山羊分成３组,第１组（ｎ＝６）为对照组,第２组（ｎ＝１２）静注大肠杆菌内毒素复制休克模型,第３组（ｎ＝１２）在第２组基础上提前１０ｍｉｎ静注山莨菪碱性注射液,检测１２ｈ内不同时间血清丙二醛（ＭＤＡ）含量,总ＳＯＤ,Ｍｎ－ＳＯＤ,ＣｕＺｎ－ＳＯＤ,ＣＡＴ和血液ＧＳＨ－Ｐｘ活性,结果表明,第２组血清ＭＤＡ含量明显增加（Ｐ〈０．０１）,血清Ｔ－ＳＯＤ,Ｍｎ－ＳＯＤ,ＣｕＺｎ－ＳＯＤ,ＧＳ 相似文献
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饮用天然高钼水诱发的钼中毒耕牛主要生物酶测定 总被引：8，自引：0，他引：8
本研究对高钼饮水诱导钼中毒的耕牛及对照牛的血浆及组织（肝脏、肺脏、肾脏、脾脏、心脏、肌肉）中超氧化物歧化酶（ＳＯＤ）、黄嘌呤氧化酶（ＸＯＤ）及谷胱甘肽过氧化物酶（ＧＳＨ－ＰＸ）等３种生物酶活性进行了对比分析测定。结果表明,血浆和组织中ＳＯＤ、ＸＯＤ及ＧＳＨ－ＰＸ均随着钼中毒的加重其活性显著下降。这３种生物酶是钼中毒比较灵敏且相关较大的生化指标,对钼中毒具有一定诊断价值;测定ＳＯＤ。ＸＯＤ、ＧＳＨ－ＰＸ可作为钼中毒监测的重要手段。 相似文献
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１９９８年１０月至１９９９年３月,在宁夏吴忠宁兰奶牛繁殖场,对试验组（ｎ＝１５）乳牛按每１００ｋｇ混合精料添加含Ｇｅ＾１３２促长素１．５ｋｇ,对照组（ｎ＝１５）添加畜禽用微量元素添加剂,共饲喂１８２ｄ。试验组与对照组相比,每头乳牛多产奶２５．５ｋｇ,可提高血清ＧＳＨ－ＰＸ、ＣｕＺｎ－ＳＯＤ的活性,降低血清ＭＤＡ的含量,提高全血Ｇｅ＾１３２、Ｓｅ、Ｚｎ、Ｃｕ的含量,提高乳牛越冬抗春乏的能力及繁殖性能 相似文献
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将６０例役牛原发性前胃弛缓根据临床症状分为消化不良型（３３例）和外感型（２７例），以１３例健康役牛作为对照，研究了原发性前胃弛缓和自由基代谢相关指标超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＨＳ－Ｐｘ）、丙二醛（ＭＤＡ）及其胃肠道激素胃泌素（ＧＡＳ）、胃动素（ＭＴＬ）的关系。结果表明，原发性前胃弛缓病牛不论消化不良型还是外感型，血清ＳＯＤ与ＧＳＨ－Ｐｘ活力较健康对照组牛显著降低（Ｐ〈０．０５或Ｐ〈０．０１），血清ＭＤＡ含量较对照组显著升高（Ｐ〈０．０５）；两种类型前胃弛缓病牛血浆ＧＡＳ、ＭＴＬ均较健康对照极显著降低（Ｐ〈０．０１），消化不良型前胃弛缓病牛血清ＧＡＳ和ＭＴＬ又明显低于外感型前胃弛缓病牛（Ｐ〈０．０５）。提示役牛原发性前胃弛缓的发生、发展与体内自由代谢失去平衡和胃肠道激素的变化有一定相关性。 相似文献
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３５周龄海兰种蛋鸡１５０只，随机分成５组，每组３０只，第Ⅰ、Ⅱ、Ⅲ、Ⅳ组于基础日粮中分别添加β－羧乙基锗倍半氧化物（Ｇｅ－１３２）５０、１００、１５０、２００ｍｇ／ｋｇ，第Ⅴ组作为对照组，只喂基础日粮。持续饲喂２５ｄ。于试验前及试验后第５、１０、１５、２０日分别测定血锗含量、血清ＳＯＤ活性、全血ＧＳＨ－Ｐｘ活性以及血清ＬＰＯ含量。试验结果：（１）第Ⅰ～Ⅳ组鸡血锗含量比对照组（Ⅴ）显著增多（Ｐ＜０．０１），而第Ⅰ～Ⅳ组间未见明显规律性剂量依赖关系；（２）第Ⅰ～Ⅳ组鸡血清ＳＯＤ活性和全血ＧＳＨ－Ｐｘ活性比对照组（Ⅴ）明显增强，而血清ＬＰＯ含量却显著降低（Ｐ＜０．０１）；（３）血锗含量与血清ＳＯＤ和全血ＧＳＨ－Ｐｘ活性呈正相关（ｒ＝０．４６３和０．５２９），而与血清ＬＰＯ含量呈强负相关（ｒ＝－０．７５１）。试验结果表明，Ｇｅ－１３２能明显地增强鸡体抗氧化系统功能。 相似文献
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选用１日龄肉用雏鸡３００只，随机分为４组：Ｉ组为健康对照；Ⅱ、Ⅲ组喂１００ｐｐｍ锗─１３２（Ｇｅ－１３２）；Ⅲ、Ⅳ组人工感染马立克氏病病毒（ＭＤＶ）。经６０ｄ临床观察并每隔１０ｄ用电子自旋共振波谱（ＥＳＲ）法测定胸腺、法氏囊及脾组织中自由基含量，同时检测全血ＧＳＨ－Ｐｘ、血清ＳＯＤ活性及血清丙二醛含量。结果，健康雏鸡全血ＧＳＨ－ｐｘ活性均值为７．９３８７±１．２３７８Ｕ／ｍＬ血清ＳＯＤ活性为８０．４６４３±１．０９８７ｎＵ／ｍＬ血清丙二醛含量为２．９９７８±０１７６０ｎｍｏｌ／ｍＬ；健康雏鸡胸腺、法氏囊及脾组织中自由基平均含量分别为：０．２７±０．０４、０．３４±０．０８、０．２８±０．０６相对浓度（×１０１０ｓｐｉｎｓ／ｇ湿重）。通过与健康对照组比较，人工感染ＭＤＶ雏鸡全血ＧＳＨ－ｐｘ和血清ＳＯＤ活性明显降低，而上述各组织中自由基和血清丙二醛含量则显著增多。Ⅲ、Ⅳ两组对比结果表明：Ⅲ组的全血ＧＳＨ－ｐｘ和血清ＳＯＤ活性高于Ⅳ组，组织中自由基和血清丙二醛含量却低于Ⅳ组，从而证实Ｇｅ－１３２能增强人工感染ＭＤＶ雏鸡的抗氧化功能。 相似文献
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饲料锌水平对肉用仔鸡肝脏中含锌酶活性的影响 总被引：7，自引：0，他引：7
选择１０日ＡＡ肉用仔鸡３００只，随机分成６组，每组５０只。第一组为对照组，饲以含锌３０ｍｇ／ｋｇ的玉米～豆饼基础日粮，其它五组依次含锌６０、１１０１６０、２１０和２６０ｍｇ／ｋｇ。至５０日龄时，测肝脏中超氧化物歧化酶（ＳＯＤ）、乳酸脱氢酶（ＬＤＨ）、谷丙转氨酶（ＧＰＴ）、碱性磷酸酶（ＡＫＰ）的活性和肝组织中的含锌量。结果表明，在试验条件下，ＳＯＤ活性随饲料锌水平的提高基本不变，ＬＤＨ、ＡＫＰ、ＧＰＴ活性及锌含量随饲料锌水平的提高而提高。 相似文献
9.
马立克氏病病毒感染雏鸡的SOD活性变化 总被引：8，自引：4，他引：4
１日龄肉用ＡＡ雏鸡被马立克氏病病毒（ＭＤＶ）强毒人工感染后，马立克氏病（ＭＤ）发病率６２％，死亡率３４％；脾脏、胸腺、法氏囊、心脏、肝脏、肾脏和性腺超氧化物歧化酶（ＳＯＤ）活性与健康雏鸡相比，Ｔ－ＳＯＤ和Ｍｎ－ＳＯＤ活性均有显著降低（Ｐ＜０．０５），而Ｃｕ，Ｚｎ－ＳＯＤ活性无显著变化（Ｐ＞０．０５）。 相似文献
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硒,维生素E对鸡体脂质过氧化的影响 总被引：18，自引：0，他引：18
２１日龄健康肉鸡８０只,随机分为４组（每组２０只）,分别在基础饲料中添加维生素Ｅ（ＶＥ）＋硒（Ｓｅ）、ＶＥ、Ｓｅ和不添加任何物质。ＶＥ添加量为１００ｍｇ／ｋｇ,Ｓｅ添加量为０．５ｍｇ／ｋｇ。试验期为５周。试验结束,立即采取血液、肝脏、胸肌、腿肌样品,分别于２ｈ内和冷藏（２℃）４８ｈ后检测其谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）和超氧化物歧化酶（ＳＯＤ）的活性以及丙二醛（ＭＤＡ）含量。结果表明,添加ＶＥ和／或Ｓｅ的３个试验组肉鸡的ＧＳＨ－Ｐｘ、ＳＯＤ活性均显著高于对照组,ＭＤＡ含量显著低于对照组,提示饲料中添加ＶＥ和／或Ｓｅ,均可增强鸡体的抗氧化效果。ＶＥ和Ｓｅ联用显示明显的协同效应。 相似文献
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在低磷地区以分组对比的试验方法 ,用不同含磷量的牧草在同等条件下喂饲两组低磷奶牛 ,定期采样分析 ,对各组奶牛血钙、血磷、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH- Px)、丙二醛 (MDA)及牧草、土壤中磷含量、喂饲后奶牛生产性能等进行测定和观察。结果表明 :(1)在预试验期 ,对牧草施撒磷肥 ,收获后 ,土壤和牧草二者含磷量均明显提高。在其后的几年间再次测定该两项指标仍处于较高水平 (P<0 .0 5 ) ,后效应明显。 (2 )对低磷奶牛喂饲富磷牧草后 ,其血清磷由1.0 2 mm ol/ L提高到 2 .0 5 m mol/ L,两组差异极显著。 (3)对照组与试验组随着喂饲时间的延长 ,血清中抗氧化指标变化明显 ,试验组抗氧化能力增强 ,SOD、GSH- Px活性增强 ;MDA含量降低。(4)从对奶牛生产性能的测定看出 ,试验组生产性能有较大幅度地提高。 相似文献
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本试验旨在研究围产前期饲粮阴阳离子差(DCAD)对产后奶牛血清生理生化指标及犊牛血清抗氧化指标的影响。试验采取单因子随机区组试验设计,选取2~4胎次、体重相近、预产期相近的围产前期(产前28天)中国荷斯坦奶牛20头,分为4组,每组5头。4组分别在围产前期饲喂DCAD为+262.31、+130.26、+78.51、+6.67 mmol/kg(干物质基础)的饲粮,阴离子盐的添加量分别为0、15.0、21.9、29.1 g/kg。试验期49 d,其中预试期7 d,正试期42 d。结果表明:降低奶牛产前饲粮DCAD能显著提高其血清钙含量(P0.05);显著降低尿液p H(P0.05);显著提高产后血清维生素D含量(P0.05);显著提高犊牛血清抗氧化能力(P0.05);但对奶牛血清肿瘤坏死因子、甲状旁腺素、降钙素和β-羟丁酸含量没有显著影响(P0.05)。因此,添加阴离子盐降低产前奶牛饲粮的DCAD可以促进其血清钙稳态,诱发机体轻度代谢性碱中毒从而减少产褥热的发病率,并提高犊牛的抗氧化能力。本试验条件下,15.0 g/kg(干物质基础)为阴离子盐最佳添加量。 相似文献
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为进一步研究低磷引起血红蛋白尿症的发病机理,对低磷奶牛血清生化指标进行了检测。采用分组对比的方法,测定各组奶牛血清若干生化指标。结果,血尿组,低磷组,对照组血清无机磷含量差异显著。 相似文献
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Fifteen cows with milk fever were treated with 500ml of 40 % calcium borogluconate (group A) administered intravenously. Fifteen other cows with milk fever received the same treatment, supplemented with 500ml of 10 % sodium phosphate administered intravenously, and 80g calcium as calcium lactate and 70g inorganic phosphorus as sodium phosphate administered orally in drinking water. The cows were monitored and blood samples collected for 3 days to measure the concentrations of total and ionized calcium, inorganic phosphorus and magnesium and the activity of creatine kinase. The two groups did not differ significantly with respect to the course of the disease. In each group 14 cows were cured. A rapid and significant increase in serum calcium concentration from the hypo- to the hypercalcaemic range occurred in both groups within 10min of the start of treatment, followed by a slow and steady decrease to the hypocalcaemic range. Calcium lactate did not prevent the calcium concentration from returning to the hypocalcaemic range, and the calcium profiles of the two groups did not differ significantly. As expected, treatment had little effect on the concentration of inorganic phosphorus in group A. In group B, treatment caused a rapid increase in the concentration of inorganic phosphorus to a maximum 20min after the start of treatment. This was followed by a slow decrease in the phosphorus concentration to the normophosphataemic range. Our findings confirmed that combined intravenous and oral administration of sodium phosphate in cows with periparturient paresis attributable to hypocalcaemia and hypophosphataemia results in a rapid and sustained increase in serum phosphorus, but not in serum calcium concentration. This modified therapy did not improve the success rate of milk fever treatment and further studies are needed to improve treatment of periparturient paresis. 相似文献
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Relationship between serum TNF activity and insulin resistance in dairy cows affected with naturally occurring fatty liver 总被引：2，自引：0，他引：2
Ohtsuka H Koiwa M Hatsugaya A Kudo K Hoshi F Itoh N Yokota H Okada H Kawamura S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):1021-1025
To clarity the relationship between tumor necrosis factor (TNF) and insulin resistance in dairy cows affected with fatty liver, naturally occurring cases were investigated. The affected cows were classified into following three groups according to histopathologic findings of the liver: mild fat droplet deposition (group 1; n=11), severe fat droplet deposition (group 2; n=10), and cloudy swelling (group 3; n=8). Serum TNF activities in Group 2 (8.67 +/- 2.16 U/ml) and Group 3 (11.65 +/- 1.92 U/ml) were significantly higher than that in Group 1 (3.57 +/- 0.81 U/ml) (p<0.05). The insulin-tolerance tests showed that the insulin-stimulated glucose disposal rates (GDR) in Group 2 (27.6 +/- 7.8%) and Group 3 (15.8 +/- 9.1%) were significantly lower than that in Group 1 (41.7 +/- 9.8%). There was a significant negative correlation between serum TNF activity and GDR in affected cows (r=-0.56, p<0.01). These results indicate that serum TNF activity is correlated with insulin resistance in cows with fatty liver. 相似文献
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Grünberg W Constable P Schröder U Staufenbiel R Morin D Rohn M 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2005,19(6):894-898
Abnormal phosphorus homeostasis occurs in dairy cows with an abomasal displacement or volvulus. The goal of this study was to identify potential mechanisms for hypophosphatemia and hyperphosphatemia in cows with a left displaced abomasum (LDA), right displaced abomasum (RDA), or abomasal volvulus (AV). Accordingly, the results of preoperative clinicopathologic analyses for 1,368 dairy cows with an LDA (n = 1,189), RDA, or AV (n = 179) (data set 1) and for 44 cows with an AV (data set 2) were retrieved. Laboratory values were compared by Student's t-tests, and correlation and regression analyses were performed. Thirty-four percent of the animals from data set 1 (463/1,368) were hypophosphatemic (serum phosphorus concentration ([Pi]) < 1.4 mmol/L), and 9% (122/1,368) were hyperphosphatemic ([Pi] >2.3 mmol/L). Serum [Pi] was significantly lower (P < .05) in cows with an LDA (1.60 +/- 0.53 mmol/L; mean +/- SD) than in cows with an RDA or AV (1.85 +/- 0.68 mmol/L). For cows with an LDA, [Pi] was correlated with serum urea nitrogen concentration ([SUN]) (r = 0.34) and serum concentration of magnesium ([Mg]) (r = 0.20). For cows with an RDA or AV, linear correlations existed between [Pi] and [SUN] (r = 0.45), [Mg] (r = 0.43), and serum chloride concentration ([Cl]) (r = -0.27). Stepwise logistic regression analysis indicated that low [SUN] and the diagnosis of an LDA had the strongest associations with hypophosphatemia. In cows with hyperphosphatemia, [Pi] was most strongly associated with azotemia. In cows with an AV, the strongest correlations with [Pi] were found for [SUN] and serum creatinine. We conclude that hypophosphatemia in cows with an LDA is primarily due to decreased feed intake. In contrast, hyperphosphatemia in cattle with an RDA or AV appears to result from dehydration and decreased renal blood flow. 相似文献
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选择产奶量、胎次、分娩时间相近的荷斯坦奶牛12头,采用单因子随机分组设计,试验分A、B、C3组,A组为对照组饲喂基础日粮,B组为基础日粮+100g脂肪酸钙,C组为基础日粮+200g脂肪酸钙。每周测定一次血清钙、磷和羟脯氨酸的含量。结果奶牛血清磷和羟脯氨酸的含量差异不显著(P>0.05),添加200g脂肪酸钙,血清钙在4周后显著升高(P<0.05)。表明:脂肪酸钙不影响奶牛血清磷和血清羟脯氨酸的含量。在日粮中添加200g脂肪酸钙4周以后,有利于促进机体对钙的吸收。 相似文献
20.
本试验探讨了细胞因子含量的变化与体细胞数的关系,确定其变化是否由乳房炎引起,为今后乳房炎的诊治提供参考依据。依据奶牛生产性能测定体细胞数的检测结果,将82头泌乳奶牛分为低体细胞组（体细胞评分≤2分）、中体细胞组（29〈体细胞评分≤6分）和高体细胞组（体细胞评分〉6分）,分别检测各组奶牛血清和乳清中细胞因子TNF-a、IL-6的含量,对结果采用SPSS分析。结果显示,随着体细胞数的增加,血清和乳清中TNF—a的含量呈上升趋势;乳清样本各组间TNF-a含量差异显著,高体细胞组显著高于低体细胞组（P〈0．05）,中体细胞组TNF—a的含量极显著高于低体细胞组（P〈0．01）。血清样本中IL-6的含量差异显著,中体细胞组血清中IL-6的含量显著高于低体细胞组（P〈0．05）,高体细胞组与低体细胞组血清中IL-6的含量差异接近显著（P值为0．059）。 相似文献