共查询到16条相似文献，搜索用时 140 毫秒
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试验旨在建立结肠小袋纤毛虫RPMI 1640培养方法,为进一步研究其生活史及致病机制奠定基础。研究不同淀粉含量、不同血清含量、不同初始pH及不同接种量下RPMI 1640培养结肠小袋纤毛虫的培养效果。结果发现,用RPMI 1640培养结肠小袋纤毛虫,最适宜的淀粉含量为30 mg/安瓿,血清含量为20%,pH为7.0;种群自然增长率和种群所达到的最高种群密度随接种量的不同而明显改变。RPMI 1640培养基可用于结肠小袋纤毛虫的体外培养。 相似文献
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目的观察结肠小袋纤毛虫（Balantidium coli）在体外培养条件下的生长情况。方法取粪样分别置于4℃～8℃冰箱，28℃和37℃恒温培养箱中，观察记录虫体数量，统计不同温度条件下滋养体生存时间。28℃恒温条件下，将粪液加入不同稀释液（蒸馏水、生理盐水及洛克氏液）中，观察滋养体的活力及数量；采用同样的观察方法，统计滋养体在双相培养基以及免血水培养基内的生存时间。在28℃和37℃恒温条件下，分别观察滋乔体在RSS培养液（Ringer液＋血清＋米粉）及不加米粉的RSS培养液内的生存时间。结果结肠小袋纤毛虫滋养体在体外的最适生存温度为28℃，在洛克氏夜中生存时间明显长于生理盐水和蒸馏水，在RSS培养基及双相培养基中可在培养24h时达到高峰。在含有诺氟沙星的兔血水培养基中，滋养体的数量可在培养后96h达到高峰，生存时间可达到180h。结论结肠小袋纤毛虫体外培养受很多因素的影响，有待于进一步研究。 相似文献
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十种药物对结肠小袋纤毛虫疗效的体外试验 总被引：1，自引：0，他引：1
本实验以结肠小袋纤毛虫在培养基中对十种药物的抵抗力的观察，结果表明，不同浓度的药物对结肠小袋纤毛虫影响不同，浓度大的影响也大，浓度小的影响则小。经实验证实，5％浓度的甲硝咪唑对结肠小袋纤毛虫的药效最佳，其次为5％浓度的呋喃唑酮，再次为5％浓度的新诺明，其余浓度的药物虽有影响，但不甚明显。十种药物对结肠小袋纤毛虫杀灭效果初筛结果依次为：甲硝咪唑、呋喃唑酮、新诺明、土霉素、左旋咪唑。 相似文献
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通过结肠小袋纤毛虫对甲硝唑、氟苯尼考和血虫净（三氮咪）的急性毒性试验,以机率单位法获得半数致死浓度（LC50）,结果显示：甲硝唑、氟苯尼考对结肠小袋纤毛虫2h的半数致死浓度（LC50,2h）分别是457．8mg／L,1705mg／L,12h的半数致死浓度（LC50,12h）分别是25．64mg／L、973．8mg／L。血虫净（三氮咪）12h和24h半数致死浓度（LC50）分别是331．8mg／L、295．4mg／L。甲硝唑是杀灭结肠小袋纤毛虫的理想药物。 相似文献
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双抗夹心—ELISA诊断牛隐孢子虫病 总被引：15，自引：1，他引：14
为了建立双抗体夹心—ＥＬＩＳＡ检测牛粪便中隐孢子虫卵囊抗原的方法。采用抗小球隐孢子虫（Ｃ．ｐａｒｖｕｍ）卵囊壁单克隆抗体,经对６０头份牛粪便样本分别进行抗酸染色和双抗夹心—ＥＬＩＳＡ检测,结果抗酸染色法检出１２头份有隐孢子虫卵囊,而ＥＬＩＳＡ除对抗酸染色阳性的１２份粪样判为阳性外,还对抗酸染色阴性的４份粪样判为阳性,且不与牛球虫、牛结肠小袋纤毛虫发生类属反应。此外,本试验在稀释液中加入ＥＤＴＡ,并增加了反应温度,使得试验在抗体包被板并封闭后３０ｍｉｎ结束整个检测过程。结果表明,双抗体夹心—ＥＬＩＳＡ是敏感性高、特异性强的诊断牛隐孢子虫病的方法。 相似文献
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湖南省规模化猪场结肠小袋纤毛虫感染情况调查 总被引：1，自引：0，他引：1
目的为了解湖南省规模化猪场结肠小袋纤毛虫的感染情况。方法采用沉淀法检测了12个规模化猪场1097头生猪的粪便样品。结果12个猪场全部都检测到了结肠小袋纤毛虫,场阳性率为100%;各猪场的感染率为22.2%~83.1%。不同生长阶段的感染率为:种公猪41.3%,繁殖母猪40.0%,哺乳仔猪10.1%,保育猪26.8%,育肥猪53.7%。在检测的1 097份粪便样品中,有416份阳性,感染率37.9%。结论12个猪场各生长阶段的猪均有结肠,小袋纤毛虫感染以育肥猪最为严重,种猪次之,哺乳仔猪则较低。 相似文献
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A cross-sectional survey was performed on 20 pig breeding farms in southern Hesse, central Germany, to evaluate the prevalence and age-dependent occurrence of intestinal protozoan parasites in unweaned piglets. Faecal samples of 514 clinically unaffected piglets of different age (< 1 to 5-7 weeks) were examined using the sodium acetate-acetic acid-formalin (SAF) concentration technique. Infections with the following protozoan species were detected: Balantidium coli (16 of 20 farms), Entamoeba sp. (15), Jodamoeba sp. (14), Isospora (I.) suis (9), Chilomastix sp. (6) and Eimeria spp. (6). The protozoan species differed in the start and course of (oo)cyst excretion. I. suis oocysts and Jodamoeba cysts were detected already in the first week of life whereas shedding of the other parasites started later on. The prevalence of Isospora oocyst excretion increased to a maximum (18%) in 2-3 weeks old animals followed by a sharp decline. The proportion of Balantidium, Entamoeba or Jodamoeba positive suckling piglets continously increased until the age of 5-7 weeks to 60%, 52% and 22%, respectively, whereas that of Chilomastix positive animals remained on a low level of 8-12% independent of the age. Eimeria oocysts were found transiently in the faeces of 1-4 weeks old piglets. 相似文献
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目的摸清重庆市荣昌县生猪的肠道寄生虫感染情况,从而为有效防控猪的肠道寄生虫感染提供依据。方法荣昌是我国著名地方猪“荣昌猪”的故乡,我国养猪大县之一,作者于2007年4月至2007年11月,在该县20个镇选择具有代表性的规模化猪场、散养户和屠宰场,对种公猪、母猪、仔猪及肥育猪肠道寄生虫感染情况和屠宰场宰杀的肥猪肠道寄生虫虫体感染情况进行了调查。共检查了832份集约化饲养猪的粪便、l133份农户散养猪的粪便,并检查了200头屠宰肥猪的肠道。结果13．1％的集约化饲养猪感染猪蛔虫,5．4％感染猪鞭虫．14．6％感染食道口线虫,14．8％感染球虫（包括艾关尔球虫及等孢球虫）,3．6％感染结肠小袋纤毛虫。对散养猪．这些数值分别是6．7％,4．8％,23．2％,13．8％,及4．9％。4％的宰杀肥猪检出猪蛔虫,3．5％检出猪鞭虫,52．0％感染食道口线虫。混合感染很常见。结论本项调查表明,荣昌生猪的寄生虫感染较为严重,应引起有关方面的足够重视。应尽快开展猪肠道寄生虫感染的综合防控工作,以保障养猪业的健康快速发展。 相似文献
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Alizadeh AR Alikhani M Ghorbani GR Rahmani HR Rashidi L Loor JJ 《Journal of animal physiology and animal nutrition》2012,96(3):466-473
Safflower seed has the highest concentration of linoleic acid among 80 oilseeds but little information exists on the effective use of SS for lactation cows. It was hypothesised that a diet supplemented with an Iranian SS variety (IL-111) in combination with fish oil (FO) would result in higher concentrations of trans-18:1 (including vaccenic acid) and conjugated linoleic acids in milk fat than feeding an unsupplemented control diet. Our objective was to determine the effects of feeding diets containing: (i) Control: (C); (ii) 25 g of roasted SS IL-111 (RSS); (iii) 20 g FO and (iv) 25 g RSS + 10 g FO (RSS + FO) per kilogram of dietary DM on feed intake, ruminal fermentation, milk production and fatty acid profile. Eight multiparous Holstein cows were used in a replicated 4 × 4 Latin square design study. The experiment had four periods of 21 days. Milk Fat percentage was lower (p < 0.01) with FO supplementation and averaged 19.0 and 21.5 g/kg milk with FO and RSS + FO compared with 30.3 and 32.5 g/kg with C and RSS. Feed intake also was lower (p < 0.01) with FO vs. C (23.1 vs. 24.5 kg/day) but feeding RSS resulted in greater feed intake compared with other treatments (26 kg/day). Despite lower feed intake with FO, milk production did not change from controls but feeding RSS + FO resulted in greater milk yield than controls (42.6 vs. 39.3 kg/day). Ruminal pH was greater (p < 0.01) in cows fed FO than other treatments. Supplemental FO alone or in combination with RSS resulted in dramatic increases (p < 0.01) in c9,t11-18:2 in milk fat (12.7 and 13.2 g/day vs. 5.8 and 7.02 with C and RSS). It was surprising to note that 25 g/kg RSS can improve feed intake. 相似文献