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1.
Barnett J Ainsworth H Boon JD Twomey DF 《Veterinary journal (London, England : 1997)》2008,176(2):251-253
Increased mortality was observed in a group of 2-3-week old goslings. At post mortem examination, gross lesions included congestion and haemorrhages in the viscera. Histopathological lesions included generalised congestion of tissues with multifocal bacterial colonies, an acute septic fibrinoid splenitis, and an acute septic hepatitis, all consistent with acute septicaemia. Gram-stained sections of liver and lung confirmed the presence of Gram-positive bacteria and Streptococcus gallolyticus subsp. pasteurianus (Streptococcus bovis biotype II/2) was isolated in pure growth from two of the goslings, and in mixed growth from a third. 相似文献
2.
Sasaki E Osawa R Nishitani Y Whiley RA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1467-1470
A total of 23 Streptococcus gallolyticus strains, consisting of 12 strains from feces of healthy animals and 11 from clinical cases of human or cow mastitis milk, were examined genealogically. Four strains of S. bovis "biotype II/1" and 3 strains of S. equinus, the closely related organisms to S. gallolyticus, were also analyzed for outgroup comparison. Neither the amplified ribosomal DNA restriction analysis (ARDRA) nor the randomly amplified polymorphic DNA (RAPD) analysis that had been designed to recognize S. gallolyticus strains virulent in pigeons could differentiate clinical strains from the others of S. gallolyticus. No correspondence between the DNA profile in either analysis and the host animal species was detected. 相似文献
3.
The cell wall protein profiles of 56 isolates of Streptococcus gallolyticus of differing virulence for pigeons were compared by SDS-PAGE. Additionally, Western blot analysis was performed on the cell wall proteins of 14 strains using sera of pigeons, experimentally infected with A(+)T1 or A(-)T2 strains of S. gallolyticus. The profile of silver stained gels exhibited a complex array of 20-50 bands ranging from less than 6.5-210kDa. A band with molecular mass of 114kDa was only observed in isolates that belonged to the highly virulent A(+)T1, A(+)T2, A(+)T3 and A(-)T1 culture supernatant groups. A band with a slightly higher molecular mass (115kDa) as well as a 207kDa band were only detected in isolates that belonged to the moderately A(-)T3 or low A(-)T2 virulent culture supernatant groups. The 114 and 115kDa band were recognised by all homologous and heterologous pigeon sera used whereas the 207kDa band was only recognised by sera of pigeons infected with a A(-)T2 strain. These findings may indicate that the 114, 115 and 207kDa bands are useful as additional virulence associated markers for pigeon S. gallolyticus strains. 相似文献
4.
Fourteen pigeon Streptococcus gallolyticus strains of differing virulence, were tested for their ability to adhere to immobilised fibronectin, collagen types I, III and IV. Eight, 2 and 13 strains were able to bind fibronectin, collagen types III and IV, respectively. None of the strains adhered to collagen type I. Heat treatment, proteolytic digestion or periodate treatment reduced the binding of S. gallolyticus to fibronectin and collagen type IV, suggesting that surface receptors contain proteins and carbohydrates. Although binding to these extracellular matrix proteins can play a role in the pathogenesis of streptococcosis in pigeons, binding properties could not be related to virulence, indicating that other factors determine differences in virulence among pigeon S. gallolyticus strains. Adhesion to collagen type IV may account in part for the distribution pattern of the lesions observed in naturally and experimentally infected pigeons. 相似文献
5.
OBJECTIVE: To identify Actinobacillus spp isolates recovered from fetuses and pericardial fluid from horses affected with mare reproductive loss syndrome (MRLS) and determine whether these bacterial species are the same as those isolated from clinically normal horses. SAMPLE POPULATION: Isolates of actinobacilli recovered from 18 horses with pericarditis and 109 fetuses aborted by mares affected by MRLS. Procedures-Actinobacillus spp isolates were identified to the level of species or subspecies by use of conventional phenotypic tests and biochemical and enzyme test kits. The 16S rRNA gene from selected isolates was amplified, purified, and sequenced. Sequence data were compared with sequence data for actinobacilli in GenBank. RESULTS: Of the 109 isolates obtained from fetuses, 14 were Actinobacillus equuli subsp equuli, 65 were A equuli subsp haemolyticus, 28 were Bisgaard taxon 10-like bacterium, and 2 were Actinobacillus genomospecies 1. Of the 18 isolates from horses with pericarditis, 4 were A equuli subsp equuli, 13 were A equuli subsp haemolyticus, and 1 was Bisgaard taxon 10-like bacterium. Comparisons with published data and GenBank data revealed that the isolates recovered from horses with MRLS were the same as those isolated from the oral cavity or alimentary tract of healthy horses. CONCLUSIONS AND CLINICAL RELEVANCE: Actinobacillus spp isolates recovered from fetuses and pericardial fluid samples of horses affected by MRLS in 2001 to 2003 were identical to Actinobacillus spp found in the oral cavity and alimentary tracts of healthy horses. 相似文献
6.
We investigated the ability of a high virulence (STR 357) and a low virulence (STR 598) strain of Streptococcus gallolyticus to attach to the intestinal tract of pigeons. For that purpose, first of all, two groups of six pigeons were anesthetized and ligatures were placed at the beginning of duodenum, jejunum, ileum, and colon. The obtained intestinal loops of the birds of the first and second group were injected with S. gallolyticus strains STR 357 and STR 598, respectively. At 15, 30, and 60 min postinoculation, two pigeons of each group were euthanatized and the various intestinal loops were sampled for histologic, immunohistochemical, and electron microscopic examination. Both the high and low virulence strains were able to adhere to the intestinal mucosa. Indeed, all samples dearly showed numerous coccal-shaped bacteria that stained positively with S. gallolyticus antiserum and were lining up against the intestinal epithelium. Likewise, on electron microscopic examination, cocci were seen in the mucus covering the intestinal epithelium. Second, the association of S. gallyticus strains of differing virulence with the intestinal tissue was determined quantitatively. Experiments were performed as described above. The number of S. gallolyticus bacteria that adhered to the intestinal epithelium was determined by plating out 10-fold serial dilutions of the segments. No significant differences in the number of adhered bacteria were found between the strains of high and low virulence. 相似文献
7.
S. gallolyticus, formerly known as S. bovis is known since 1988 as a facultative pathogen of racing pigeons. Important clinical signs include acute mortality, inability to fly, lameness, weight loss and slimy green diarrhea. A pathognomonic sign at post mortem examination is the presence of well circumscribed areas of necrosis in the pectoral muscle. Furthermore tenosynovitis of the supracoracoid muscle and arthritis of the knee, shoulder and hock can be observed. In one study S. gallolyticus septicaemia was diagnosed in 10% of necropsied pigeons. Since S. gallolyticus was also isolated from nearly 40% of clinical healthy pigeons it is regarded as a facultative pathogen. Various biotypes, serotypes and culture supernatant phenotypes can be distinguished. Supernatant phenotypes are identified on the basis of the presence of either a T1, T2 or T3 protein triplet and the presence or absence of an extracellular A protein. S. gallolyticus strains with A protein are highly virulent, while strains with only T3 or T2 protein are of moderately or low virulence respectively. Fimbriae are only seen in highly virulent and some of the moderately virulent strains. Possible virulence factors include survival in macrophages, adhesion to cells and toxin production. Infection with serotype 1 and 2 induces some degree of protection against re-infection with serotype 1, which offers perspectives for the development of a vaccine. Experimentally ampicillin, doxycycline and erythromycin have shown therapeutic effects. For the treatment of clinical cases the use of ampicillin is advocated, together with hygienic measures, such as the use of grid floors and avoiding overcrowding. 相似文献
8.
Five clinically normal chickens from three farms (farm A, farm B, and farm C), for a total of 15 clinically normal chickens, were examined bacteriologically. In a similar manner, five dead chickens with lesions of peritonitis from each of the same three commercial egg-laying operations were selected for bacterial culturing. Escherichia coli were isolated from the cloaca in 14 of 15 healthy chickens and from all 15 chickens with peritonitis. Oviducts of normal chickens did not contain E. coli (0/15) whereas oviducts from 13 of 15 hens with peritonitis were positive for this pathogen. No lesions and no E. coli (0/15) were found in the peritoneal cavity of healthy hens, but peritonitis lesions from 13 of 15 dead chickens yielded E. coli. On farm A and farm B, a flock consisted of all chickens within a single house and all chickens in each flock were of the same age and same genetic strain. In flock 1 from farm A, all five E. coli isolates from the oviduct and all five isolates from the peritoneal cavity were serogrouped as O78; contained the virulence genes iroN, sitA, iutA, tsh, and iss; and belonged to phylogenetic group A. In flock 2 from farm B, all four E. coli isolates from the oviduct and all four isolates from the peritoneal cavity were serogrouped as O111; contained virulence genes iroN, sitA, iutA, traT, iss, and ompT; and belonged to phylogenetic group D. These data suggest that all chickens with peritonitis in a single flock on farms A and B were likely infected by the same E. coli strain. Escherichia coli isolates from the magnum and peritoneum had the same serogroup, virulence genotype, and phylogenetic group, which is consistent with an ascending infection from the oviduct to the peritoneal cavity. 相似文献
9.
Between 1998 and 2001, mortalities due to verrucous endocarditis were experienced at several mink farms. Gram-positive cocci were isolated from the endocardium of all the animals examined but not always from other internal organs. Almost all the isolates were identified as Streptococcus bovis and only a few isolates belonged to other Streptococcus species. Typing by pulsed-field gel electrophoresis of a selection of isolates revealed several patterns and several different clones. Attempts to reproduce disease by the injection of cultures of a field isolate into healthy mink failed. 相似文献
10.
Yo Okuda Masaaki Ono Isao Shibata Shizuo Sato Hiroomi Akashi 《Journal of veterinary diagnostic investigation》2006,18(2):162-167
The fiber gene sequence and pathogenicity of the serotype-1 fowl adenovirus (FAdV-1) isolated from gizzard erosions and from clinically normal chickens were compared among isolates. The FAdV-99ZH strain, which induced gizzard erosions, had a nucleotide sequence of the long fiber gene that was different from that of the Ote strain, which did not induce gizzard erosions. The differences could be distinguished by use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The long fiber gene of 16 FAdV-1 isolates from gizzard erosions and 10 FAdV-1 isolates from the feces of clinically normal chickens was examined by use of PCR-RFLP analysis. All 16 FAdV-1 isolates from gizzard erosions had the same restriction patterns as those of strain 99ZH; however, 10 FAdV-1 isolates from normal chickens were classified into 3 groups. Specific-pathogen-free (SPF) chickens were inoculated orally with 2 FAdV-1 isolates from gizzard erosions or 3 FAdV-1 isolates from clinically normal chickens to determine the pathogenicity of each strain. Two of 2 FAdV-1 isolates from gizzard erosions induced gizzard erosions. Two of 3 FAdV-1 isolates from normal chickens had the same PCR-RFLP patterns as those of the Ote strain, but did not induce any gizzard erosions. However, 1 FAdV-1 isolate from clinically normal chickens had the same PCR-RFLP pattern as that of strain 99ZH and induced gizzard erosions. These results indicate that there are FAdV-1 strains that have different pathogenicity; one strain induces gizzard erosions, and the other does not. Use of PCR-RFLP analysis of long fiber genes may be able to distinguish between these two strains. 相似文献
11.
Proteolytic zymograms of Staphylococcus hyicus subsp. hyicus isolated from pigs, chickens and cows 总被引：1，自引：0，他引：1
The extracellular proteases of Staphylococcus hyicus subsp. hyicus were assayed by a zymogram showing caseinolysis and gelatinolysis. Four bands were associated with caseinolysis or with gelatinolysis. The patterns shown by strains isolated from pigs, chickens and cows were compared; isolates from pigs differed from those isolated from chickens or cows but strains isolated from diseased and healthy pigs could not be differentiated. 相似文献
12.
Devriese LA Hommez J Laevens H Pot B Vandamme P Haesebrouck F 《Veterinary microbiology》1999,70(1-2):87-94
Aesculin-hydrolyzing, catalase-negative, gram-positive cocci isolated from subclinical intramammary infections in dairy cows were identified to species level using growth characteristics and biochemical activity. The results indicated that the aesculin-hydrolyzing cocci associated with this type of infection are a very heterogenic group. S. uberis strains, including inulin- or beta-glucuronidase-negative isolates, accounted for only about one-third of the collection, and Enterococcus faecalis strains for one-fifth. Other species of some importance included (in descending order of isolation frequency) Aerococcus viridans, Streptococcus pluranimalium, Lactococcus garvieae, Streptococcus bovis and Streptococcus gallolyticus. 相似文献
13.
The presence and quantity of protein A in Staphylococcus hyicus subsp. hyicus isolates were examined by an enzyme-linked immunosorbent assay (ELISA) and immunoelectron microscopy. Cell-bound protein A was demonstrated in 45 (94%) of 48 isolates from diseased pigs and in 113 (86%) of 132 isolates from healthy pigs by ELISA using peroxidase-conjugated rabbit antibody, but was not found in isolates from chickens and cows. Most of these swine isolates contained about 100 to 300 ng of cell-bound protein A/ml. Extracellular protein A was not detected in any isolates from pigs, chickens or cows. In the immunoelectron microscopy assay, swine isolates were labeled with goat anti-mouse IgG conjugated to colloidal gold particles, but chicken and cow isolates were not labeled. 相似文献
14.
Urosevic M Lako B Milanov D Urosevic I Aurich C 《Berliner und Münchener tier?rztliche Wochenschrift》2010,123(9-10):365-368
Uterine microbiology, antimicrobial susceptibility and endometrial cytology were investigated in a total of 51 mares with fertility problems from 16 different stud farms in Serbia. Uterine cultures were performed after collection with a double guarded uterine swab, and endometrial cytology was evaluated after collection of endometrial cells with a special device (cytology brush). In 21 of 51 mares, at least one bacterial species was isolated from the uterus; the most frequent were Streptococcus equi subsp. zooepidemicus (13 isolates) and E. coli (four isolates). All isolates of Streptococcus equi subsp. zooepidemicus were susceptible to penicillin. Results from endometrial cytology were inconsistent; in 17 animals with positive bacteriological culture, cytology was not altered. It can be concluded that in Serbia, as in many other contries, Streptococcus equi subsp. zooepidemicus is the main cause for equine endometritis. It can be easily diagnosed by uterine culture but endometrial cytology does not always prove the existence of an endometrial infection with this agent. 相似文献
15.
Irfan Ahmad MIR Bablu KUMAR Anil TAKU Farah FARIDI Mohd. Altaf BHAT Naseer Ahmad BABA Tahir MAQBOOL 《Journal of Equine Science》2013,24(3):53-55
Present study was undertaken to study the prevalence of β-haemolytic streptococci in
equine of northern temperate region of Jammu and Kashmir, India. One hundred and forty one
samples were collected in duplicate from nasopharyngeal tract of diseased (53) and
apparently healthy equine (88) for isolation and direct PCR. A total of 77 isolates of
streptococci were recovered from 141 samples with an overall prevalence rate of 54.60%.
Out of these 77 isolates, 52 were from diseased and 25 from apparently healthy animals. Of
the 77 isolates, 4 were identified as Streptococcus equi subsp.
equi, 56 as S. equi subsp.
zooepidemicus and 17 as S. dysgalactiae subsp.
equisimilis. Thus the overall prevalence of S. equi subsp.
equi, S. equi subsp. zooepidemicus
and S. dysgalactiae subsp. equisimilis was 2.83, 39.71
and 12.05% respectively. The sensitivity of the PCR for the detection of S.
equi species was found higher when attempted from direct swab samples. 相似文献
16.
在成都、重庆等１０个市、地、县，从患有慢性呼吸道疾病的鸡场采集１０８份样品，分离的２７个菌株，经Ｌ型细菌检验，理化特性鉴定，符合支原体的生物学特性。通过血清学鉴定（生长抑制试验），证实分离的支原体与鸡毒支原体国际代表株Ｓ６为同一血清型。人工复制试验结果，表明分离的鸡毒支原体ＳＭＧ－１和ＳＭＧ－２具有高致病性，感染鸡出现典型的慢性呼吸道病症状 相似文献
17.
Frank LA Kania SA Hnilica KA Wilkes RP Bemis DA 《Journal of the American Veterinary Medical Association》2003,222(4):451-454
OBJECTIVE: To determine frequency with which Staphylococcus schleiferi could be isolated from dogs with pyoderma and antimicrobial susceptibility patterns of isolates that were obtained. DESIGN: Prospective study. ANIMALS: 54 dogs with a first (n = 14) or recurrent (40) episode of pyoderma. PROCEDURE: Specimens were obtained and submitted for bacterial culture. Isolates were identified as S schleiferi on the basis of growth and biochemical characteristics. Two isolates were submitted for DNA sequencing to confirm identification. Methicillin susceptibility was determined by means of disk diffusion with oxacillin-impregnated disks. RESULTS: 3 of 14 dogs examined because of a first episode of pyoderma and 12 of 40 dogs examined because of a recurrent episode of pyoderma were receiving antimicrobials at the time of specimen collection. Staphylococcus schleiferi was not isolated from any dog with first-time pyoderma but was isolated from 5 dogs with recurrent pyoderma that were not receiving antimicrobials at the time of specimen collection and 10 dogs with recurrent pyoderma that were receiving antimicrobials. Nine isolates were identified as S schleiferi subsp schleiferi, and 6 were identified as S schleiferi subsp coagulans. All S schleiferi subsp schleiferi isolates were resistant to methicillin, but only 2 S schleiferi subsp coagulans isolates were. Two methicillin-resistant isolates were also resistant to fluoroquinolones, and 1 isolate had intermediate susceptibility to fluoroquinolones. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that S schleiferi subsp schleiferi and S schleiferi subsp coagulans may be isolated from dogs with recurrent pyoderma. Although isolates from dogs with pyoderma were frequently resistant to methicillin, multiple drug resistance was uncommon. 相似文献
18.
Thu WL Wang CH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):325-328
Subgroup J avian leucosis virus (ALV-J) causes great economic losses in the poultry industry. One in 3 grandparent farms was closed due to ALV-J infection in 1998 in Taiwan. The remaining 2 farms were forced to import breeding chicks from different breeding companies afterwards. We report on the ALV-J infection status among these breeders, their progeny and Taiwan native chickens during 2000-2002. The weekly mortality for the male line among the infected breeders was higher than that for the female line. Sixty-three percent (5/8) of the broiler flocks were infected with ALV-J. The surface (SU) portion of the env gene from the ALV-J field isolates was cloned and sequenced. The phylogenetic results show that all of the isolates fell into 2 clusters. Unexpectedly, the isolates from the same breeds fell into different clusters, with a cluster including isolates from different breeding companies. ALV-Js from native chickens crossbred with imported chickens were placed into the same clusters as those from the imported breeds. The high similarities observed in different ALV-J isolates suggest that different ALV-Js were mixed in the pedigree generations in different breeding lines. 相似文献
19.
The objective of this study was to isolate and identify Streptococcus bovis from yak, and detect their hemolytic, pathogenicity and antimicrobial susceptibility. 45 fecal samples collected from the diarrheal yaks in Aba state in Northwest Sichuan province were used to isolate Streptococcus bovis with sheep blood culture media under 37℃ and 5% CO2 condition cultured for 24 h. The isolated bacteria were identified by 16S rRNA amplification and sequencing. Total 14 Streptococcus bovis were identified, among which, 8 Streptococcus lutetiensis strains and 6 Streptococcus gallolyticus strains. 9 Streptococcus bovis strains showed α hemolytic, and 5 Streptococcus bovis showed β hemolytic. Both Streptococcus lutetiensis and Streptococcus gallolyticus caused the experimental mice mild diarrhea. The isolated 14 Streptococcus bovis were sensitive to penicillin, cefotaxime, vacomycin, acetylspiramycin, ciprofloxacin, rifampicin, teicoplanin, ampicillin and gentamicin, and highly tolerant to streptomycin, kanamycin, lincomycin, erythrocin, tetracycline and clindamycin.This study illustrated the biological characteristics of the isolated Streptococcus bovis from yak, which made the basis for the prevention and control of diseases caused by Streptococcus bovis. 相似文献
20.
本研究旨在从腹泻牦牛粪便中分离鉴定牛链球菌,并分析其溶血性、对小鼠的致病性及对抗菌药物的敏感性。将川西北阿坝州45份腹泻牦牛粪便于血平板上划线,37℃、5% CO2培养24 h分离细菌,经16S rRNA序列扩增测序和系统发育分析鉴定出14株牛链球菌,其中8株巴黎链球菌,6株解没食子酸链球菌巴氏亚种;9株呈α溶血,5株呈β溶血。分离鉴定的牦牛源巴黎链球菌和解没食子酸链球菌巴氏亚种能引起试验小鼠的轻度腹泻;药敏试验结果显示分离菌株对青霉素、头孢噻肟、万古霉素、乙酰螺旋霉素、环丙沙星、利福平、替考拉宁、氨苄西林和庆大霉素共9种抗生素高度敏感,对链霉素、卡那霉素、林可霉素、红霉素、四环素和克林霉素耐药率较高。本研究阐明了牦牛源链球菌的部分生物学特性,为该病的防控奠定了基础。 相似文献