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《黑龙江畜牧兽医》2017,(17)
为了提高蓝舌病病毒(BTV)的分离效率,2015年在师宗县设置监控点,选择蓝舌病抗体阴性的10头牛和5只羊作为监控动物,5—10月份每周采血1次,每次每头动物分别采集常规全血、EDTA抗凝血和肝素抗凝血各1份,检测蓝舌病抗体、蓝舌病病毒核酸并进行病毒分离。结果表明:蓝舌病病毒核酸阳性可以持续4~5周,蓝舌病抗体阳性可以持续5~6周。BTV核酸开始转为阳性到核酸转阳后的第1周和第2周采集的肝素抗凝血,或者蓝舌病抗体转阳前1周到蓝舌病抗体转阳和蓝舌病抗体转阳后1周采集的肝素抗凝血BTV分离效果最佳。说明通过蓝舌病抗体检测或蓝舌病病毒核酸检测确定分离蓝舌病病毒最佳分离时间是可行的。 相似文献
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蓝舌病是由蓝舌病病毒引起反刍动物的严重传染病。以口腔、鼻腔和胃肠道黏膜发生溃疡性炎症变化为特征,主要侵害绵羊。世界动物卫生组织（OIE）将其列为A类疫病。蓝舌病在养殖牛、羊、鹿等动物的时候,是应当值得注意的。1病原蓝舌病是由蓝舌病病毒引起的主要发生于反刍动物的传染病。 相似文献
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蓝舌病是一种经济上重要的反刍动物病毒性疾病.蓝舌病是由蓝舌病病毒引起的,属于我国一类动物传染病,对多种反刍动物健康造成威胁,并可造成巨大的社会经济损失.本文对蓝舌病的历史和世界分布、经济影响、临床症状和鉴别诊断进行综述,旨在为蓝舌病的诊断提供参考. 相似文献
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蓝舌病是由蓝舌病病毒引起的一种主要发生于绵羊的传染病,临床上比较少见。但其他反刍类家畜也可能被感染。该病最早于1876年发现于南非的绵羊,1906年定名为蓝舌病。1949年后,该病在全世界50多个国家或地区陆续发生。我国于1979年在云南首次发现蓝舌病,并分离出蓝舌病病毒,从而确定了蓝舌病的存在。随后湖北、四川、安徽、山西也相继报导了该病。该病分布于全球大多数地区,成为世界性危害的虫媒传染病。 相似文献
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《畜牧兽医科技信息》2020,(4)
正牛蓝舌病是由蓝舌病病毒引起的一种非接触性传染病,患病动物症状为水肿、充血、食欲不振。本文阐述了牛蓝舌病的病原、发病机制、临床症状、诊断方法和防治手段,意在为牛蓝舌病的综合防治提供理论依据。1病因牛蓝舌病的病原是蓝舌病病毒,该病毒属于呼肠孤病毒 相似文献
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用禽类成髓细胞病毒（AMV）反转录酶将蓝舌病病毒RNA反转录成cDNA,以dATP、dGTP、dCTP、dTTP和bio-11-dUTP作为TaqDNA聚合酶的底物,应用PCR技术合成了生物素化蓝舌病病毒核酸探针,经斑点杂交试验证明,合成的探针为蓝舌病病毒特异性核酸探针。 相似文献
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蓝舌病是由蓝舌病毒(BTV)引起的一种非接触性传染病,主要侵害反刍动物,库蠓是蓝舌病毒传播最重要的媒介生物。本研究基于尖喙库蠓地理分布数据及环境数据,利用最大熵生态位模型(MaxEnt)对尖喙库蠓在中国大陆的分布情况进行了预测,并评估了环境变量对尖喙库蠓分布的影响。结果显示,最适合尖喙库蠓生存的地区主要分布在中国的西北和东北部分地区,以及南方的大部分地区;Jackknife分析结果显示,土壤有效水含量(25%)、最冷月最低温(18.1%)、最干月降雨量(18%)、平均最大风速(13.2%)是影响尖喙库蠓分布最主要的4个环境因子,其中最干月降雨量是模型中影响尖喙库蠓分布的最重要环境变量。本研究首次将这种生态位模型用于预测中国库蠓的分布,为蓝舌病的预防控制工作提供参考依据,同时也为库蠓监测管理提供信息。 相似文献
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竞争酶联免疫吸附试验检测蓝舌病抗体的研究 总被引：5，自引：0，他引：5
用已研制的BTV－11型VP7单克隆抗体建立了竞争酶联免疫吸附试验（C－ELISA）检测蓝舌病抗体的方法，并与琼脂免疫扩散试验（AGID）进行了检测比较，C－ELISA特异性强，不与相关环状病毒发生交叉反应，敏感性比AGID高。用研究制备的C－ELISA诊断试剂盒和美国、澳大利亚制备的诊断试剂盒对1377份临床样品的检测，以及对实验动物人工感染后抗体动脉检测。三种诊断试剂盒检测结果一致，且重复性好。本研究建立的蓝舌病C－ELISA是一种特异性强、敏感性高的蓝舌病抗体检测方法。 相似文献
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为了解近年来云南省师宗县蓝舌病病毒流行情况,2012年在师宗县五龙乡建立了10头蓝舌病血清学阴性黄牛的监控动物群。从2012年5~10月,每周采血1次,11~12月,每月采血1次,采用C-ELISA进行血清学监测。8月开始动物血清学检测结果转阳性,至11月,监控动物全部转为阳性。用转阳前1周、转阳本周、转阳后2~13周的经处理的红细胞静脉接种鸡胚,收获鸡胚肝脏,用PBS悬浮捣碎的鸡胚肝脏,上清接种于C6/36细胞一代、BHK-21三代后,出现细胞病变(cytopathic effect,CPE)。采用RT-PCR方法,针对蓝舌病较为保守的血清型群特异片段VP7设计了2对引物,扩增其相应片段。结果显示,共分离到86份疑似分离物,其中67份疑似分离物细胞培养液上清经RT-PCR扩增,均扩增出1156 bp片段,初步确认为蓝舌病病毒。采用国际24个蓝舌病标准毒及24个标准阳性血清对86份疑似分离物及其对应血清进行细胞微量中和试验,67份毒株为蓝舌病病毒,与RT-PCR结果一致。通过对2份经中和试验定型为BTV-1、BTV-16分离株的VP2基因测序分析发现,BTV-1株序列与同型Y863(登录号:KC879616)参考毒株的同源性为92%,BTV-16株序列与登录号为AB686221的毒株同源性为99%。结果表明共分离到67株蓝舌病毒株,分离株主要为BTV-1、BTV-9、BTV-16三个血清型。 相似文献
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Swiss Bluetongue surveillance from mid 2007 to mid 2008 was a combination of monthly bulk milk testing of 200 cattle herds in zones of higher risk for vector presence and intensification of passive clinical surveillance. The spatial scan statistic and Moran's I statistic were used to determine clustering of surveillance data. The results show a high level of surveillance intensity for BT in Switzerland in 2007. In the region encompassing the Cantons of Aargau, Basel-Landschaft, Basel-Stadt and Solothurn, where cases were detected in 2007, the surveillance was significantly higher than in the rest of Switzerland. Six cases of Bluetongue were detected within the surveillance system. The prevalence estimates 9.62% (95% CI = 3.25%-18.85% versus 0.98% (95% CI = 0.2%-2.22%) were also significantly higher in the area with higher surveillance intensity. Spatial variation in surveillance data should to be considered if a disease event is analysed on a national scale. The spatial variation of prevalence estimates should be considered in future Bluetongue surveillance in Switzerland. 相似文献
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De Ankan Das Tapan Kumar Chand Karam Debnath Bikas Chandra Dey Saikat Hemadri Divakar Barman Nagendra Nath Chaudhary Jitendra Kumar Muthuchelvan Dhanavelu Saxena Arpit Tewari Neha Chauhan Ankita Lohumi Ankita Biswas Sanchay Kumar 《Tropical animal health and production》2019,51(1):261-265
Tropical Animal Health and Production - Bluetongue (BT) is a notifiable multiple species transboundary viral disease of domestic and wild ruminants. Though the disease is enzootic in India, little... 相似文献
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Maclachlan NJ 《Preventive veterinary medicine》2011,102(2):107-111
Bluetongue is an arthropod-transmitted viral disease of ruminants and certain other animals that was recognized and described more than 100 years ago in southern Africa. Bluetongue virus (BTV) infection of ruminants and vector Culicoides insects is enzootic throughout tropical and temperate regions of the world; however, there have been drastic recent regional alterations in the global distribution of BTV infection, particularly in Europe since 1998. Multiple novel BTV serotypes also have been detected since 1998 in the south-eastern United States, apparently encroaching from the adjacent Caribbean ecosystem, and novel serotypes of BTV have been identified recently in other historically enzootic regions of the world, including the Middle East and Australia. It has been proposed, but certainly not proven, that global climate change is responsible for these events. BTV infection of ruminants is often subclinical, but outbreaks of severe disease occur with regular frequency especially at the upper and lower limits of the virus' global range where infection is highly seasonal - occurring in the late summer and autumn. Bluetongue disease results from vascular injury, likely through a process analogous to that of human hemorrhagic viral fevers in which production of vasoactive mediators from virus-infected macrophages and dendritic cells results in enhanced endothelial paracellular permeability with subsequent vascular leakage and hypovolemic shock. 相似文献
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Gür S 《Tropical animal health and production》2008,40(3):217-221
Bluetongue virus (BTV) is a vector-borne disease of ruminants disseminated in the tropic and sub-tropic zone of the world.
It is also an important problem in the Middle East. Three serotypes (4–9–16) of the virus have been isolated so far in Turkey.
Gazella subgutturosa subgutturosa (Goitred Gazella) is native species of Anatolia. Due to risk of extinction, captured Gazelles have been taken under protection
in a restricted area at Ceylanpinar state farm. In this study, the presence of Bluetongue virus (BTV) was investigated serologically
in 82 Goitred Gazella, 684 sheep and 100 cattle. Seropositivity rates in Gazella, sheep and cattle were detected as 40.2%,
295% and 88%, respectively. 相似文献
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Racloz V Straver R Kuhn M Thur B Vanzetti T Stärk KD Griot C Cagienard A 《Schweizer Archiv für Tierheilkunde》2006,148(11):593-598
Bluetongue (BT) is a vector-borne animal disease of economical importance due to the international trade restrictions likely to be put into place in a country once the infection is discovered. The presence of BT and its vectors in countries adjacent to Switzerland stresses the need of implementing a surveillance system and to raise disease awareness among potential stakeholders. A national survey in Switzerland 2003 indicated freedom of Bluetongue virus (BTV), although a single individual of the main BT vector Culicoides imicola was caught in the canton of Ticino. The survey also demonstrated that potential BT vectors, C. obsoletus and C. pulicaris are locally abundant in Switzerland. Therefore, a new surveillance method based on sentinel herds in high risk areas was implemented in 2004 for the early detection of both an incursion of BT vectors into Switzerland, and potential virus circulation among cattle. 相似文献
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By the distribution of a questionnaire between all Swiss cattle practitioners it was possible to investigate abortions and other animal health problems related to Bluetongue vaccination 2009. The questionnaire helped to obtain plausibility and timely relation of the reported disorders. 58 abortions in cattle and different herd health problems could be examined. Because there is no possibility to show that a vaccination itself leads to an abortion the results of proven causes of abortions prior and after Bluetongue vaccination were compared regarding their diagnosis. Due to the fact that diagnosis and solving rate of abortions did not differ before and after vaccination, the vaccination itself cannot be responsible for the abortions. Evaluation of different herd health problems showed that Bluetongue vaccination was not responsible for these disorders which often existed already prior to vaccination. Herd health problems generally have multifactorial causes what makes it difficult to asses the effect of Bluetongue vaccination in some cases. 相似文献