共查询到19条相似文献，搜索用时 265 毫秒
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以MM-3基因工程活菌苗口服免疫妊娠73d、83d、103d母猪各4头,口服及肌注免疫妊娠92d母猪各5头,用间接BA-ELISA法检测血清及乳清中IgG、IgA和IgM型抗K_(88ac)及LTB抗体的动态变化.结果表明,无论口服还是肌注免疫母猪,抗K_(88ac)及抗LTB抗体均极显著增高.肌注以IgG增加为主,口服以IgA增加为主.肌注免疫IgG型抗K_(88ac)抗体滴度极显著地高于口服免疫(P<0.01);口服免疫IgA型抗LTB抗体滴度显著高于肌注免疫(P<0.05).口服及肌注免疫乳清中抗K_(88ac)及抗LTB抗体滴度很高,达1:3200～204800,但在1周内即降低,约减少一半(约3～5个滴度),2～3周内各型抗体下降缓慢,维持在对照组周内水平,4周后达最低水平.妊娠不同时期口服免疫,其血清中抗K_(88ac)抗体的动态变化相似,但乳汁中抗体则差异非常明显,妊娠83d和92d免疫组IgG、IgA和IgM均显著增加,且高于103d组(P<0.01)和73d组(P<0.05).口服免疫的最佳时间为分娩前26～30d,肌注免疫最佳免疫时间为分娩前21d. 相似文献
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从88例病猪分离到1177个菌株,对其中42例病猪的138个菌株,用自制的大肠埃希氏菌抗血清鉴定出O_8:K_(87),K_(88ac)血清型是引起河南省乳猪腹泻的优势血清型.调查了八个地区,其中七个地区分离到O_8群,说明分布很广,主要危害3～6日龄的初生乳猪.O_(141):K_(85ac),K_(88ac)和O_6:K_(2ac)在较小范围内流行,主要危害3～4日龄的新生乳猪。O_(139):K_(82)为引起70日龄左右的仔猪水肿病的血清型.大多数含有K_(88)抗原和能溶解绵羊红血球,有的能产生肠毒素,有较强的致病力。 相似文献
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分别于不同日龄采集海兰褐蛋用型雄性雏鸡和AA肉用型雏鸡血清，测定其对新城疫病毒(NDV)HI抗体水平．以此确定抗NDV母源抗体的衰减规律及半衰期。结果表明，雏鸡出壳后抗体水平持续升高，到4日龄左右达到最高峰．随后快速下降．三周后抗体几乎消失。蛋用型雏鸡血清中对NDV母源抗体的半衰期为2．19d左右。肉用型雏鸡血清中对NDV母源抗体的半衰期为3．10d左右。 相似文献
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猪瘟母源抗体在仔猪体内持续时间的研究 总被引：11，自引：0，他引：11
仔猪吮吸免疫猪瘟疫苗母猪的初乳后,监测母源抗体在仔猪体内的消长规律,以探索仔猪猪瘟疫苗最佳首免日龄.试验选择3个场共12头母猪,产前136～140天进行猪瘟兔化弱毒(细胞苗)疫苗接种,4毫升/头.通过猪瘟抗体酶联免疫吸附试验(竞争性ELISA)检测其所产仔猪7、14、21、28、35和42日龄时血清中抗体水平.结果发现,3个猪场试验仔猪母源抗体下降幅度不一,甲场第28天降到最低,丙场第21天降到最低,之后其抗体水平均出现升高现象.但3个猪场之间不同日龄仔猪抗体水平差异较大. 相似文献
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抗大肠杆菌K88卵黄粉对断奶仔猪生长和腹泻的影响 总被引：1，自引：0，他引：1
采用单因子随机化设计,将60头健康断奶仔猪按体重和性别一致的原则分成3个组,每组4个重复,每个重复5头猪。各组仔猪分别采食3种试验日粮(基础日粮;基础日粮+卵黄粉0.1%;基础日粮+卵黄粉0.3%)。结果表明,添加含抗大肠杆菌K88的卵黄粉可显著提高断奶仔猪的生产性能,其中断奶后前2周的平均日增重分别比对照组提高了11.34%和1.03%,饲料报酬分别改善4.65%和7.44%。添加卵黄粉也显著降低断奶仔猪采食量,添加0.1%卵黄粉增加仔猪采食量6.35%,0.3%的卵黄粉则使采食量降低6.35%。结果还显示,使用含抗大肠杆菌抗体K88的卵黄粉添加剂显著降低了腹泻率,改善断奶仔猪健康状况。 相似文献
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用猪瘟、猪肺疫、猪丹毒三联疫苗和猪丹毒1a、2型氢氧化铝吸附甲醛灭活疫苗分别免疫断奶仔猪，采用Dot-PPA-ELISA监测免疫猪血清抗体变化。结果，弱毒疫苗免疫后第7d猪丹毒血清抗体效价开始升高，第2～3周达最高值；灭活疫苗免疫后第3～4周抗体效价达最高值，且灭活疫苗免疫猪的抗体水平明显高于三联疫苗免疫猪。免疫6周后，用C43004(1a型)、C43179(1b型)和C43-12(2型)3株不同血清型的猪丹毒混合强毒同时对不同抗体效价的免疫猪和对照猪进行了皮肤内接种攻毒，并根据其皮肤和体温反应确定1：32为猪能抵抗混合强毒攻击的抗体保护效价。经对44头50～60日龄未免疫的断奶仔猪血清检测，其抗体效价在1：32以下的猪占88．36％，278头免疫猪血清的抗体效价在1：32或以上的猪占92．81％。表明Dot-PPA-ELISA适用于猪群猪丹毒的免疫监测和免疫力测定。 相似文献
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分离、纯化产肠毒素大肠杆菌(ETEC)K88ac菌毛蛋白，将其免疫产蛋鸡，经喷雾干燥制备抗ETEC菌毛抗原特异性卵黄抗体粉。间接ELISA法检测卵黄抗体效价，试验结果表明，喷雾干燥(140℃进气，65℃出气)对卵黄抗体活性无影响，加工前后抗体滴度均为1：1280；卵黄抗体粉在90℃干热条件下处理5min后抗体效价下降50％，而90℃湿热条件下处理5min抗体效价无明显变化，但处理15min后下降75％；经过1h的干热或湿热处理，抗体均基本失活；4℃和常温下放置半年对卵黄抗体粉活性无影响；体外抑制粘附试验表明，抗K88卵黄抗体能抑制K88对肠上皮细胞的粘附。 相似文献
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为了摸清猪瘟母源抗体的作用情况 ,了解乳猪实施接种猪瘟三联苗后产生免疫力的确切效果 ,笔者于 2 0 0 0年 3～ 6月对我州坝固种畜场的 4头孕猪 (大约克 )及其仔猪 (与长白杂交的后代共 36头 )进行了被动与主动免疫效果的监测。应用正向血凝试验检测生产母猪及其仔猪血清中的抗猪瘟抗体滴度 ,同时还检测实施主动免疫后乳猪不同时期血清中抗猪瘟抗体的消涨变化。发现孕猪有抗猪瘟抗体的比率为75 % ;其仔猪通过母体获得抗猪瘟抗体的比率为 41 67% ;对未满双月的仔猪接种猪瘟三联苗后 ,其血清中产生有效抗猪瘟抗体的比率比通过母体被动免疫而获… 相似文献
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本试验通过研究免疫状况良好的母猪群所产仔猪母源抗体的获得方式,仔猪体内母源抗体的持续时间,仔猪不同免疫程序的免疫效果,发现初生仔猪遵守被动免疫传递规律,仔猪体内的口蹄疫抗体水平也有自己的消长规律:哺乳后3～7d抗体水平达到最高,35日龄后开始下降,63日龄左右抗体保护率已经低于60%。所以,仔猪口蹄疫疫苗首次免疫的时间应定在35日龄,49日龄还需加强一次;疫苗的剂量:首免为1头份(2mL),加强时为2头份(3mL)。 相似文献
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试验旨在了解产蛋鸡对犬瘟热病毒(canine distemper virus,CDV)抗原的免疫反应及其血清抗体和卵黄抗体的消长规律,为制备卵黄抗体提供依据。将CDV接种Vero细胞和DF1细胞进行传代并测定其病毒滴度,选取细胞病变出现早、病毒滴度高的Vero细胞毒作为接种病毒抗原免疫蛋鸡。每10 d免疫蛋鸡1次,第4次免疫后每30 d加强免疫1次。免疫前及免疫后每10 d采血分离血清,每5 d收集鸡蛋提取卵黄抗体,用琼脂扩散法和间接ELISA法测定其抗体水平。结果显示,血清抗体比卵黄抗体的滴度高,两者呈正相关性,卵黄抗体出现较血清抗体迟3~5 d,卵黄抗体在第3次免疫后3~5 d就已达到较高水平,此时即可开始收集鸡蛋制备卵黄抗体。 相似文献
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禽霍乱免疫母鸡血清抗体和蛋黄抗体消长规律及其相互关系的研究 总被引：3，自引：0，他引：3
通过对禽霍乱菌苗免疫产蛋母鸡的血清和蛋黄的抗体定时检测后发现，免疫母鸡的血清抗体和蛋黄抗体的升降趋势基本一致（ｒ＝０．９４），但后者较前者迟后３～６ｄ；血清抗体和蛋黄抗体的滴度分别在加强免疫后的１～３ｄ和３～６ｄ都有不同程度下降，加强免疫前的滴度越高，免疫后的滴度下降幅度越大；蛋黄抗体水平普遍比血清抗体水平低，两者间的差异极显著（Ｐ＜０．０１），这可能是与禽多杀性巴氏杆菌本身的主要抗原成分（ＴＩ抗原）在鸡体内主要产金ＩｇＭ有关。 相似文献
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de Souza EM Starke-Buzetti WA Ferreira FP Neves MF Machado RZ 《Veterinary parasitology》2004,122(1):67-78
Humoral immune response of water buffalo naturally infected with Toxocara vitulorum was monitored using three different antigens of this parasite in serum and colostrum of buffalo cows and calves. Soluble extract (Ex) and excretory/secretory (ES) larval antigens and perienteric fluid antigen (Pe) of adult T. vitulorum were used to measure the antibody levels by an indirect ELISA. Serum of 7-12 buffalo cows for the first 365 days and colostrum of the same number of buffalo cows for the first 60 days of parturition, and serum of 8-10 buffalo calves for the first 365 days after birth were assayed. The ELISA detected antibodies against all three T. vitulorum antigens in the colostrum and serum of 100% of buffalo cows and calves examined. The highest antibody levels against Ex, ES and Pe antigens were detected in the buffalo cow sera during the perinatal period and were maintained at high levels through 300 days after parturition. On the other hand, colostrum antibody concentrations of all three antigens were highest on the first day post-parturition, but decreased sharply during the first 15 days. Concomitantly to the monitoring of immune response, the parasitic status of the calves was also evaluated. In calves, antibodies passively acquired were at the highest concentrations 24 h after birth and remained at high levels until 45 days coincidentally with the peak of T. vitulorum infection. The rejection of the worms by the calves occurred simultaneously with the decline of antibody levels, which reached their lowest levels between 76 and 150 days. Thereafter, probably because of the presence of adults/larvae stimulation, the calves acquired active immunity and the antibodies started to increase slightly in the serum and plateaued between the days 211 and 365. All three antigens were detected by the serum antibodies of buffalo calves; however, the concentration of anti-Pe antibody was higher than anti-EX and anti-ES, particularly after 90 days of age. By conclusion, the buffalo cows develop immunity and keep high levels of antibodies against T. vitulorum-Ex, ES and Pe antigens and these antibodies are transferred to their calves through the colostrum. This passively acquired immunity does not protect the calves against the acquisition of the infection, but these antibodies, passively or actively acquired, may have an important role during worm rejection by the calves and prevention of intestinal reinfection. 相似文献
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Withanage GS Sasai K Fukata T Miyamoto T Baba E 《Veterinary immunology and immunopathology》1999,67(2):185-193
The production and secretion of Salmonella enteritidis whole cell antigen-specific antibodies in the oviducts and in the serum of laying hens experimentally infected with Salmonella enteritidis, was analyzed by ELISA. The dynamics of the antibody levels in the oviducts were identical to that in the serum. Subclasses of antibodies (IgA, IgG, and IgM) in the infected hens were found to increase significantly (p < 0.01) compared to those in the control uninfected hens throughout the experiment. IgG and IgM levels in both oviducts and in sera reached to a peak by 14 days post-inoculation, and remained elevated throughout. The secretion of IgA seemed to be transient since the IgA levels increased to a peak 7 days after both primary and secondary inoculations, and declined rapidly. The elevated levels of antibodies were followed by partial clearance of Salmonella organisms from the oviducts. The present results indicate a significant local immune reaction against the Salmonella infection and suggest an association of the local antibodies with the clearance of Salmonella from the oviducts at least partially. 相似文献
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Toxocara vitulorum, a parasite of the small intestine of cattle and water buffaloes, is mainly acquired by calves via the colostrum/milk from infected cows. To understand the development of immune responses in calves, antibody levels to a soluble extract antigen (Ex) from T. vitulorum infective larvae were measured by an indirect ELISA with sera of 15 buffalo calves, which were sampled every 15 days for the first 180 days after birth and 9 buffalo cows during the perinatal period. From all serum samples examined during the first 180 days, antibody level was lowest and highest in calves at 1 day of age before and after suckling colostrum, respectively, suggesting that the origin of antibodies was the colostrum. Immediately after birth, antibody levels in suckled calves remained at high levels until day 15, began to decrease to lower levels between 15 and 30 days and remained relatively stable until 120 days. By comparing the immune responses of these animals with their parasitological status it was considered possible to determine if passively acquired or actively produced antibodies provided protection against the infection. High numbers of T. vitulorum eggs in the feces between 30 and 60 days indicated that passively acquired antibodies did not provide protection against the infection, at least during these first days, and the maximum fecal egg counts during 30-45 days were coincident with decreased antibody levels. Between 60 and 120 days, when serum antibodies were detected at reduced, but stable levels, adult nematodes were expelled from the intestines and no more T. vitulorum eggs were found, suggesting development of acquired resistance. However, the potential and functional protective role of the antibodies against T. vitulorum infection and the process of self-cure requires further investigation. 相似文献
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Transfer of maternal antibody against group A rotavirus from sows to piglets and serological responses following natural infection 总被引：1，自引：0，他引：1
An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of antirotaviral antibody in sera and faeces from pigs and used to study the dynamics of antirotaviral antibody responses in three cohorts of pigs. Piglets acquired antirotaviral antibody by sucking their dams soon after birth. Antirotaviral antibodies of IgA and IgG classes were detected in both colostrum and milk of all sows tested but IgM class antibodies were not. The antibody levels in colostrum were eight to 32 times higher than those in milk which was collected 18 days post partum. The levels of antibody in piglets' sera were comparable to those in colostrum but declined quickly to low levels by one month old. Maternal antibody was also detected in the faeces of piglets up to 18 days old. Natural rotavirus infection occurred in each of these cohorts when the geometric mean ELISA titres of maternal antibody in their sera declined to 1/1600 (by days 21, 25 and 30 for cohorts 1, 2 and 3, respectively). However, a positive correlation was not obtained between the levels of antirotaviral antibody and protection in individual litters within each of the cohort groups. In each of the cohorts, rotavirus infection usually occurred in one or two piglets first and then spread to other piglets in the same cohort. It is therefore suggested that maternally derived antibody is protective against rotavirus infection in piglets only for the first one or two weeks. Following natural infection with rotavirus, increases in serum antibodies were detected in two of the three cohorts by 20 to 30 days after the average time of onset of faecal shedding of virus.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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为研究小反刍兽疫病毒N蛋白抗体与H蛋白抗体在羊体内的代谢消长规律,试验通过建立小反刍兽疫N蛋白双抗原夹心ELISA抗体检测方法、H蛋白阻断ELISA抗体检测方法与血清中和抗体试验方法,分别检测了羊免疫疫苗后体内N蛋白抗体与H蛋白抗体在每个免疫阶段的代谢消长变化。结果表明:羊免疫小反刍兽疫疫苗后,血清内的N蛋白抗体与H蛋白抗体在6~8周时血清抗体效价较高,对应的羊体内中和抗体效价为1∶512,且效价至少可以持续到10周以上,2种抗体具有一定消长代谢规律的相关性。该研究结果也为以N抗原与H抗原为基础建立相应的抗原捕获ELISA方法、竞争ELISA检测方法、间接ELISA检测方法以及双抗原夹心ELISA检测方法奠定了一定的理论依据。 相似文献