共查询到20条相似文献，搜索用时 203 毫秒
1.
《黑龙江畜牧兽医》2015,(7)
为了研究利巴韦林对感染猫细小病毒(FPV)细胞的作用,试验采用F81猫肾细胞,在猫细小病毒感染后不同时间点收集细胞,应用MTT法检测细胞的增殖活性;利用利巴韦林毒性试验,摸索出药物对细胞的安全浓度;利用不同浓度利巴韦林处理已攻毒细胞,MTT法检测细胞的增殖活性,酶标法检测细胞氧化损伤情况。结果表明:利巴韦林能明显抑制FPV引起的细胞死亡率,病毒组过氧化氢(H2O2)含量和细胞培养液中乳酸脱氢酶(LDH)活性在各个采样时间点均显著高于对照组,显示细胞氧化损伤严重,致使LDH从细胞内渗出。病毒组细胞内超氧化物歧化酶(SOD)活性和还原型谷胱甘肽(GSH)含量均明显低于对照组,揭示病毒所引起的细胞氧化损伤进一步激发了细胞自体保护性ROS清除机制,最终导致了细胞内SOD活性和GSH含量的下降。利巴韦林药物组LDH活性和H2O2含量都呈现升高趋势,但其增长幅度明显低于病毒组;在各采样时间点,药物组细胞内SOD活性和GSH含量均明显(P0.05)高于病毒组,低于对照组。说明利巴韦林对感染猫细小病毒细胞具有一定的修复治疗效果。 相似文献
2.
《中兽医医药杂志》2020,(5)
研究桃金娘鞣质抗腹泻作用机理。以正常小鼠为模型,测试胃内容物排空率、小肠内容物推进率,观察桃金娘鞣质对胃肠推进运动的影响;以乙酸注射腹腔炎症小鼠为模型,测试血液中伊文斯蓝腹腔透入量,观察桃金娘鞣质对腹腔毛细血管通透性影响;以豚鼠离体肠道为模型,测试生理收缩反应峰值、乙酰胆碱诱导收缩反应峰值,观察桃金娘鞣质对肠道平滑肌收缩反应的影响。结果显示,桃金娘鞣质能显著抑制小鼠的胃排空运动及小肠推进运动(P0.05);显著降低腹腔炎症小鼠血液中伊文斯蓝腹腔透入量(P0.05);显著抑制豚鼠离体肠道生理收缩反应(P0.05),不能拮抗乙酰胆碱诱导的收缩反应(P0.05)。说明桃金娘鞣质主要通过抑制胃肠收缩运动或降低肠道蠕动;降低腹腔毛细血管、肠绒毛膜通透性;抑制炎症介质合成释放或抑制炎症介质泻下作用等机制产生抗腹泻作用;与阿片激动剂类药物配伍使用抗腹泻效果可能更好。 相似文献
3.
《黑龙江畜牧兽医》2015,(16)
为了研究利巴韦林对感染猫细小病毒(FPV)细胞的影响,试验将猫肾细胞(F81细胞)随机分为对照组、病毒组、药物组,采用MTT法检测细胞的生长曲线,Hochest 33258染色观察细胞的细胞核形态,流式细胞仪检测细胞周期。结果表明:Hochest 33258染色试验中药物组与病毒组差异不显著(P0.05);药物组细胞的增殖活性始终高于病毒组,但低于对照组;流式细胞仪检测利巴韦林治疗后48小时G0/G1期细胞增多,S期细胞减少,G2/M期细胞变化不大;72小时G2/M期细胞增多;药物组与病毒组细胞的流式检测在G1峰的左侧均出现典型的亚G1峰,但各时间点药物组细胞的凋亡指数均明显低于病毒组。说明利巴韦林能抑制FPV诱导的细胞凋亡,具有一定的治疗指导意义。 相似文献
4.
试验旨在研究桃金娘4种酚类成分抗猪流行性腹泻病毒(PEDV)的药理活性。以PEDV感染PK细胞为模型,采用细胞病变(CPE)效应法、MTT比色法检测细胞存活率,测定4种酚类成分半数中毒浓度(TC50)、半数有效浓度(EC50),计算药物的治疗指数(TI),分析4种酚类成分对PEDV的抑制作用。结果显示,桃金娘4种酚类成分均有不同程度抗PEDV的药理活性,鞣花酸、白藜芦醇、没食子酸、白皮杉醇的TI分别为65.48、32.59、14.58、3.74;抗病毒效应鞣花酸最好,白藜芦醇优于没食子酸,白皮杉醇最弱。结果表明,桃金娘4种酚类成分具有体外抗PEDV药理活性,鞣花酸、白藜芦醇、没食子酸具有抗猪流行性腹泻病毒药物研究和开发价值。 相似文献
5.
《中国预防兽医学报》2016,(10)
为研究猪流行性腹泻病毒(PEDV)在感染仔猪各主要器官中的增殖和分布情况,本研究根据PEDV N基因和宿主细胞GAPDH基因建立荧光定量RT-PCR法,并以该方法检测各个发病期的哺乳仔猪各器官内PEDV的病毒载量。结果表明,仔猪感染PEDV后,在49 h~72 h出现典型的猪流行性腹泻症状,肠、肠系膜淋巴结、肺等器官出现严重病变。利用本研究建立的检测方法在仔猪体内肠、肠系膜淋巴结、肺、脾、肾、心、肝这些器官中均可以检测到PEDV;其中肠、肠系膜淋巴结、肺中病毒载量最高,而且感染时间早、持续时间长。研究表明,PEDV的感染呈持续性、多脏器性,并对肠和肺有组织嗜性;结合本实验室前期的病理研究,推测其在消化器官、呼吸器官中增殖能够导致功能细胞的损伤,在免疫器官的增殖能够造成免疫抑制和混合感染。本研究为PEDV的感染特性、定植规律和致病机理的研究及分子生物学检测方法的建立奠定了基础。 相似文献
6.
试验选取昆明种小鼠,采用经口灌胃法,研究磺胺二甲嘧啶对小鼠亚慢性毒性、骨髓细胞微核和精子畸形的影响。结果显示,亚慢性毒性试验中,高、中剂量组小鼠体重与对照组和低剂量组差异极显著(P＜0.01);高剂量组雄性小鼠心脏的脏器系数与对照组和低剂量组差异显著(P＜0.05);高剂量组雄性小鼠肾脏的脏器系数显著低于对照组(P＜0.05),雌性小鼠肾脏的脏器数显著低于对照组与低剂量组(P＜0.05);小鼠骨髓细胞微核试验中高剂量组与空白对照组差异显著(P＜0.05),高、中、低剂量组间无显著差异;小鼠精子畸形试验中,高、中剂量组与空白对照组差异极显著(P＜0.01),低剂量组与空白对照组差异显著(P＜0.05),高剂量组与中、低剂量组差异显著(P＜0.05)。结果表明,高剂量的磺胺二甲嘧啶对小鼠的心脏和肾脏及微核发生率有一定影响;中剂量的磺胺二甲嘧啶对雌性小鼠的微核发生率具有一定影响;高、中、低剂量的磺胺二甲嘧啶对精子畸形率均具有一定影响。 相似文献
7.
《黑龙江畜牧兽医》2016,(22)
为了研究左旋肉碱作为新型功能性添加剂在小鼠不同生长期的体重变化情况,试验将昆明小鼠随机分为高剂量组、低剂量组和空白对照组,通过在饮水中加入不同剂量的左旋肉碱,连续测定6周的体重,分离小鼠的心脏、肝脏、脾脏、肺脏、肾脏并称取重量。结果表明:高剂量组(♀)和对照组(♀)第2周体重差异显著(P0.05);高剂量组(♂)和对照组(♂)第1周和第6周体重差异显著(P0.05);低剂量组(♂)和对照组(♂)在第2周、第3周体重差异显著(P0.05),第4,5,6周体重差异极显著(P0.01);其他与对照组之间差异不显著(P0.05);无论是雌性小鼠还是雄性小鼠,高剂量组、低剂量组和对照组脏器重均无显著性差异(P0.05);脏器系数均无显著性差异(P0.05)。说明左旋肉碱对昆明小鼠在不同生长周期的生长性能有促进作用,脏器无特殊变化。 相似文献
8.
为研究中药组方加味麻杏石甘汤对人工感染传染性支气管炎病毒(Infectious bronchitis virus,IBV)雏鸡的保护作用及其作用机制,将240只15日龄雏鸡随机分为6组:健康对照组、IBV接种组、利巴韦林治疗组及加味麻杏石甘汤高、中、低剂量治疗组。IBV接种组及各治疗组雏鸡的接种病毒量为3×10-5.68EID50/0.3 mL,于攻毒后48 h进行治疗,剂量分别为0.01%利巴韦林和0.6%、0.4%、0.2%加味麻杏石甘汤。ELISA方法检测各组雏鸡血清中细胞因子γ干扰素(interferonγ,IFN-γ)与白介素4(interleukin 4,IL-4)含量。结果显示:IBV接种组雏鸡血清中IL-4水平显著升高,IFN-γ水平显著降低;经过药物干预治疗以后,血清中IL-4水平降低,IFN-γ水平提高。各药物治疗组之间,中药高、中剂量组IFN-γ水平高于低剂量组和利巴韦林组,IL-4水平较之偏低。可见,加味麻杏石甘汤可使IBV感染雏鸡血清中IFN-γ含量上调,IL-4含量下调,其保护机制可能是通过改善IBV感染导致的Th1/Th2失衡而起作用。 相似文献
9.
将60只昆明小鼠采用分层分组法分为空白对照组（CON）、免疫功能低下组(环磷酰胺)、左旋咪唑(levamisole)阳性对照组,以及苦参碱(matrine)高剂量组、中剂量组和低剂量组,每组10只。免疫功能低下组(环磷酰胺)及matrine高、中、低剂量组4组进行免疫力低下小鼠模型的制备,之后将matrine高、中、低剂量组腹腔注射matrine,高剂量组160 mg/kg、中剂量组80 mg/kg、低剂量组40 mg/kg。连续给药7 d,测定脾/胸腺指数、耳肿胀度、T细胞增殖、血清溶血素含量、碳廓清试验和腹腔巨噬细胞增殖试验。Matrine能提高免疫低下小鼠的脾/胸腺指数,显著增加免疫低下小鼠的耳肿胀度、胸腺增殖指数,并增强免疫低下小鼠的碳廓清能力。Matrine能增强免疫低下小鼠的细胞免疫和非特异性免疫功能,但对体液免疫功能无影响。 相似文献
10.
为了研究蓝刺头黄酮对昆明小鼠血液生理、生化指标及脏器指数的影响,评价蓝刺头黄酮的安全性,试验将80只昆明小鼠分为4组,每组20只,即高、中、低剂量组(分别按体重灌胃蓝刺头黄酮400 mg/kg、200 mg/kg、100 mg/kg)和对照组(灌胃纯化水0.5 mL/只),连续用药30 d,试验结束时对小鼠进行眼球采血,测定血液生理、生化指标,处死各组小鼠,计算脏器指数并取肝脏、肾脏制备病理组织学切片,观察病理组织学变化。结果表明：高、中、低剂量组间血常规指标均无显著差异(P>0.05);高剂量组平均红细胞数显著高于对照组(P<0.05),高剂量组和中剂量组血红蛋白含量均显著高于对照组(P<0.05);各组血常规指标均在正常范围内。高、中、低剂量组间血液生化指标无显著差异(P>0.05);高剂量组总胆固醇含量显著低于对照组(P<0.05),高剂量组和中剂量组天门冬氨酸氨基转移酶活性、三酰甘油含量均显著低于对照组(P<0.05)。高、中、低剂量组间脏器指数差异不显著(P>0.05),但高剂量组肝脏、肺脏和心脏指数显著高于对照组(P<0.05... 相似文献
11.
An enzyme-linked immunospot (ELISPOT) has been developed to detect porcine epidemic diarrhea virus (PEDV)-specific antibody secreting cells (ASC) in gut associated lymphoid tissues (duodenum and ileum lamina propria and mesenteric lymph nodes) and systemic locations (spleen and blood) of conventional pigs so as to characterise the mucosal and systemic antibody response generated by the infection with PEDV. A total number of 28 eleven-day-old conventional pigs were orally inoculated with the field isolate of the PEDV strain CV-777. Diarrhea was observed in 32% of the pigs and virus shedding was demonstrated in 100% between postinoculation day (PID) 1 and 8. Serum IgG and IgA antibodies to PEDV were detected by isotype ELISA from PID 12 and 15, respectively, reaching maximum values at PID 32 (IgG) and 21 (IgA). PEDV specific IgM ASC occurred in all the tissues between PID 4 and 7, with the strongest response in the intestinal lamina propria. IgA and IgG ASC responses were evident in the intestinal lymphoid tissues from PID 21, the highest number of specific ASC corresponded to the duodenum lamina propria. In the systemic lymphoid tissues the number of IgG and IgA ASC detected were lower than in the mucosal tissues, however, in the blood, presence of IgA ASC was constantly detected from PID 14 until the end of the experiment. Memory antibody response to the PEDV was also studied by secondary in vitro stimulation of the mononuclear cells (MNC) isolated from mesenteric lymph nodes, spleen and blood. The memory B cell response was prominent at PID 21 and 25 and consisted in IgG and IgA ASC. To our knowledge, this is the first report to research into the presence and distribution of specific ASC in different locations of the systemic and the gut associated lymphoid tissues after a PEDV infection as well as the presence of memory B cells. 相似文献
12.
给8头生后3d的哺乳仔猪经口感染猪流行性腹泻病毒(PEDV)“吉”毒株,于感染后18、30、45和96h各扑杀2头,以透射电镜和扫描电镜观察了小肠粘膜上皮细胞及肠系膜淋巴结的超微结构。结果表明,小肠上皮细胞的病变因感染时间不同而有明显差异。上皮细胞的脱落和残留上皮细胞超微结构的破坏,以感染后30h最严重,病毒在这些上皮细胞内的增殖最显著。感染后45h,见有大量新生上皮细胞修补损伤的肠绒毛。感染后96h,小肠绒毛短缩、粗大乃至发生融合。实验仔猪肠系膜淋巴结内巨噬细胞和淋巴细胞的超微结构均遭到破坏,在巨噬细胞内见有PED冠状病毒粒子。 相似文献
13.
PEDV分离株S1基因的重组杆状病毒真核表达 总被引：1，自引：1，他引：0
为了研究猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)分离株(PEDV/LA/2014/02)纤突蛋白(S1)的真核表达及其反应原性,本研究利用杆状病毒真核表达系统表达出重组His-PEDV-S1蛋白。利用在线软件分析PEDV S1基因在sf9细胞内的稀有密码子,经优化密码子后的基因进行人工合成,合成后的PEDV S1基因被克隆至杆状病毒的穿梭载体(pFastBac HT A)中,转化大肠杆菌DH10Bac感受态细胞进行重组,PCR方法验证后将重组成功的杆状病毒基因组转染sf9细胞,获得包装成功的杆状病毒,该病毒进一步接种sf9细胞,显微镜观察重组病毒引起的细胞病变,RT-PCR方法验证PEDV S1基因的mRNA表达水平,SDS-PAGE、Western blotting方法验证重组PEDV S1蛋白的表达及其反应原性。结果显示,试验成功构建了重组穿梭质粒pFastBac HT A-PEDV-S1(pSL598),成功包装表达了PEDV S1的重组杆状病毒,重组杆状病毒能使sf9细胞出现细胞变大、胞内有空泡等典型病变,PEDV S1基因的mRNA获得表达,重组蛋白His-PEDV-S1在sf9细胞中得到表达,蛋白质大小为83 ku左右,主要存在于细胞沉淀中,表达的重组蛋白能与小鼠抗His抗体和猪抗PEDV阳性血清反应,说明该蛋白具有较好的反应原性。本研究为研制PEDV新流行毒株新型亚单位疫苗和防控该毒株的流行提供了材料。 相似文献
14.
猪流行性腹泻病毒(PEDV)和猪丁型冠状病毒(PDCov)都属于冠状病毒科,它们会引起仔猪腹泻、脱水的临床症状,在某些情况下还会导致死亡.本研究旨在评估PEDV和PDCov对仔猪感染后42 d的生长性能和组织增生的影响.将断奶后14 d、平均体重为(10.82±0.78)kg的225头仔猪随机分为3组,每组5个重复,每... 相似文献
15.
本试验利用猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)SC株免疫产蛋鸡,收集高免卵黄,采用水稀释法和水稀释-盐析法获得鸡源抗PEDV卵黄抗体,采用已建立的ELISA方法对卵黄抗体效价进行测定。以3日龄无母源抗体的易感仔猪为试验动物,对抗PEDV卵黄抗体的治疗效果及安全性进行测定,并进一步利用自然发病猪场的治疗效果进行验证。结果表明,经PEDV SC株细胞毒免疫的鸡可在2次加强免疫后15 d产生高水平的特异性抗体。在实验室治疗试验中,攻毒治疗组仔猪的存活率达60%,攻毒对照组存活率为20%;用于自然发病猪场时,饲喂抗PEDV卵黄抗体饲料的仔猪存活率亦为60%,而自然发病对照组的存活率为10%。以上结果表明抗PEDV卵黄抗体对感染PEDV的仔猪有一定治疗作用。 相似文献
16.
de Arriba ML Carvajal A Pozo J Rubio P 《Veterinary immunology and immunopathology》2002,85(1-2):85-97
Eleven-day-old conventionally reared piglets were inoculated orally with two different doses of the cell-culture adapted strain CV-777 of the porcine epidemic diarrhoea virus (PEDV) or the virulent isolate of the same strain and challenged with the same virulent PEDV 3 weeks later. Pigs inoculated with the two doses of the attenuated virus did not show any typical sign of the disease, and virus shedding was not frequent. In contrast, 31% of pigs exposed to the virulent PEDV developed diarrhoea and virus shedding was demonstrated in 100%. At different postinoculation day (PID) and postchallenge day (PCD) virus-specific antibody-secreting cells (ASC) in gut associated lymphoid tissues (duodenum and ileum lamina propria and mesenteric lymph nodes) and systemic locations (blood and spleen) were assessed by enzyme-linked immunospot (ELISPOT). Only a small response was detected in the groups inoculated with attenuated PEDV, whereas in the group previously exposed to the virulent virus on PID 21 a large number of IgG and IgA ASC was detected. Isotype-specific antibody responses in serum were investigated by ELISA. IgG responses were detected in all groups, although the highest response corresponded to the group inoculated with virulent virus and only this group showed an IgA response. The pigs exposed to virulent PEDV were completely protected against the challenge with a higher dose of the same virulent virus on PID 21 and none of them shed the virus. The pigs inoculated with the attenuated strain were partially protected against the challenge, and 25% of the low dose- and 50% of the high dose-exposed pigs did not shed virus after challenge. All the pigs from a control group, not previously exposed to the virus, excreted the virus in faeces. A strong positive correlation was established between protection and the ASC responses detected in gut associated lymphoid tissues and blood at the challenge day and also between protection and serum isotype-specific antibody titers on that day. In addition, the IgA and IgG ASC responses detected in the blood on PID 21 also correlated with the responses found in the gut associated lymphoid tissues. The ASC and serum antibody responses after the challenge corresponded to a secondary immune response in the groups inoculated with attenuated virus, whereas a primary response was evident in the control group. No increase was seen in any of the parameters studied in the pigs inoculated with virulent PEDV. 相似文献
17.
Experimental primary postnatal bovine viral diarrhea viral infections in six-month-old calves 总被引：1，自引：0，他引：1
C L Wilhelmsen S R Bolin J F Ridpath N F Cheville J P Kluge 《Veterinary pathology》1990,27(4):235-243
Eight clinically healthy calves were inoculated intranasally, four with either noncytopathic or four with cytopathic bovine viral diarrhea virus, and were necropsied 5 or 12 days post-inoculation. The most frequent gross lesion associated with noncytopathic or cytopathic viral infection was proximal colonic mural edema. Consistent microscopic findings were acute to subacute tracheitis, mild enterocolitis with edema, petechial hemorrhages of mesenteric lymph nodes with mild follicular lymphocytic depletion, and paracortical lymphocytic hyperplasia. At necropsy, cytopathic virus was recovered from 4/4 calves and noncytopathic virus was isolated from 2/4 calves. Neutralizing antibodies to noncytopathic bovine viral diarrhea virus were detected in the two calves from which noncytopathic virus was not recovered. Immunohistochemical analysis of lymphoid tissues demonstrated a small, randomly distributed population of mononuclear cells that contained bovine viral diarrhea viral antigen in 7/8 calves. 相似文献
18.
Bovine syncytial virus was isolated from mesenteric lymph nodes of young dairy calves, beef cattle, and mature dairy cattle. Isolations were made in cocultures of lymph node specimens and bovine embryonic lung cells or by subpassage of cocultures in which cytopathic effects were not initially detected. The bovine syncytial viral isolates after storage at -65 C for 3 months in either infected cell cultures of supernate fluids from suspensions or sonically treated infected cell monolayers were usually recovered. Bovine syncytial virus also was recovered from cell cultures after storage for 6 months at -25 C. 相似文献
19.
Samir Smeti Souha Tibaoui Juan Ramon Bertolín Yathreb Yagoubi Ilyes Mekki Margalida Joy Naziha Atti 《Journal of animal physiology and animal nutrition》2021,105(3):452-461
Despite the fact that the use of rosemary and thyme residues and essential oils in animal feeding was widely documented, that of myrtle is scarce. To test the hypothesis that myrtle essential oils (MEOs) could improve goats' carcass characteristics and meat quality traits, twenty-one male goats received a ration consisted of 40% oat hay and 60% concentrate. Experimental goat kids received the control diet supplemented with 0, 0.3 and 0.6% of myrtle essential oils (MEOs) for C, Myrt1 and Myrt2 groups respectively. The administration of MEO did not improve the daily DM intake (p > 0.05). Kids of C and Myrt2 groups had higher average daily gain than Myrt1 group (75 versus 55 g). The goats slaughtered at 19.9 kg of weight did not differ (p > 0.05) in carcass weights and carcass yield in terms of commercial dressing percentage (CDP = 41%) and real dressing percentage (RDP = 52%). The administration of MEO increased the meat polyphenol content, being higher in both Myrtle groups (87 versus. 56 μg gallic acid equivalents g-1 fresh matter, p < 0.05). Myrtle EO administration protected kids’ meat against oxidation (0.48 versus. 0.91 mg MDA/kg of meat for Myrtle and C groups, respectively, at the 9th day of storage; p < 0.05). It could be useful to include MEO as a dietary supplement in goats’ rations since it improves meat's oxidative status without negative effects on FA profile. 相似文献
20.
An immunohistochemistry technique was developed for the diagnosis of porcine epidemic diarrhea virus (PEDV). The technique was tested on formalin-fixed, paraffin-embedded intestinal tissues from piglets naturally infected with PEDV. Five different monoclonal antibodies (MAbs) were tested in this study. PEDV antigen was consistently detected in the PLP (4% paraformaldehyde, 100 mM L-lysine dihydrochloride, 10 mM sodium m-periodate in phosphate-buffered saline)-fixed PEDV-infected Vero cells or formalin-fixed, paraffin-embedded intestinal tissues from piglets naturally infected with PEDV. The C9-2-2 MAb gave the strongest reactivity and least background staining, detecting 10 of 10 infected pigs. The positive reaction was cytoplasmic. Positive enterocytes were distributed over the tip and along the sides of atrophied or fused villi in the jejunum and ileum. Positive-staining cells were not detected in the crypts. No staining was observed in cecum and colon. No positive cells were observed when the C9-2-2 MAb was reacted with the tissue sections from noninfected piglets or from transmissible gastroenteritus virus (TGEV)- and rotavirus-infected piglets. The selected anti-PEDV MAbs tested on formalin-fixed, paraffin-embedded tissue sections are useful for diagnosis when virus isolation is not available. This method would be of particular value in countries where both PEDV and TGEV are epizootic and would aid in differentiating between PEDV and TGEV infection. 相似文献