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鸡白痢沙门氏菌PCR检测技术的建立与应用 总被引：1，自引：0，他引：1
为鸡沙门氏菌的临床检测提供了一种更快速、敏感、特异的诊断,参照GeneBank中已公布的的鸡白痢沙门氏菌fliC基因序列,合成出了一对引物,使用PCR法对1株沙门氏标准菌株和其他3株非沙门氏菌标准菌株进行DNA抽提扩增并检测其敏感度。采用上述技术,对12份可疑病料及10份饲料进行PCR检测,同时与传统检测方法进行比较。结果表明1株沙门氏菌PCR产物出现600 bp的特异性DNA扩增带,而非沙门氏菌均未出现扩增条带,证明所设计引物具有沙门氏菌特异性;通过敏感度检测,此PCR体系能检出50 pg以上的细菌DNA,敏感性较高。运用PCR法阳性检出率及敏感性均高于常规检测方法。由此可见,沙门氏菌PCR检测是一种快速、敏感和高度特异的诊断方法。 相似文献
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《中国预防兽医学报》2017,(3)
为建立鸡白痢沙门氏菌与其他常见致病性沙门氏菌的血清型特异性快速PCR检测方法,本研究通过对Gen Bank中鸡白痢沙门氏菌、鸡伤寒沙门氏菌和肠炎沙门氏菌全基因组进行生物信息学分析,确定SEEP17495基因为鸡白痢沙门氏菌的检测靶基因,设计特异性引物,建立了一种能够直接从粪便样品中检测鸡白痢沙门氏菌的PCR检测方法。结果显示:该方法对于两株鸡白痢沙门氏菌均可以特异性地扩增出356 bp的目的片段,但对于7株常见非沙门氏菌致病菌和29株其他常见致病性沙门氏菌血清型的扩增结果均为阴性。该方法无论检测鸡白痢沙门氏菌纯培养菌,还是检测模拟鸡粪样品中的鸡白痢沙门氏菌,检测的灵敏度均为103cfu/m L。本研究建立的PCR检测方法具有良好的特异性和灵敏性,并且能够快速检测出粪便样品中的鸡白痢沙门氏菌,为鸡白痢沙门氏菌的检测提供了一种快速、有效的方法。 相似文献
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饲料中沙门氏菌的PCR检测方法研究 总被引：1，自引：0，他引：1
研究应用PCR技术建立饲料中沙门氏菌的快速检测方法,根椐沙门氏菌的invA基因核苷酸序列设计1对特异性引物,分别对4种沙门氏菌的标准菌株及2种非沙门氏菌株进行PCR扩增。结果:4种沙门氏菌均扩增出331bp的特异条带,非沙门氏菌皆无特异条带,特异性达100%,敏感性达104cfu/ml。DNA序列分析证实,所扩增片段为沙门氏菌的invA基因的特异性片断。本研究建立的沙门氏菌检验方法具有较高的特异性和灵敏性,invA基因的序列分析表明,其基因序列较保守,可以做为PCR检验的目的模板。 相似文献
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食源性沙门氏菌快速检测技术的应用研究 总被引：4，自引：0，他引：4
通过样品试验,得到优化的沙门氏菌检验方法,了解上海地区沙门氏菌 污染情况.在免疫胶体金技术、单抗直接ELISA、PCR法检测沙门氏菌的基础上,将 PCR法和单抗直接ELISA三种快速检测方法进行比较研究.用免疫胶体金技术、直接E LISA、PCR法对鸡蛋、水样、牛奶、猪肉、牛肉、虾仁等样品401份进行沙门氏菌检测,并与 常规国标方法对比,直接ELISA法的敏感性和特异性达100%和97.3%,免疫胶体金技术敏感 性和特异性达100%和95.3%,PCR法的敏感性和特异性均为100%.通过对样品的检测, 最终确定较优化的沙门氏菌检测程序.PCR法的特异性优于免疫胶体金技术和直接ELISA法.对大量样品检测,可采用免疫胶体金技术或直接ELISA法筛检,除去大量阴性样品,阳性样品用PCR法作进一步鉴定;需要确定血清型时则用国标法. 相似文献
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PCR技术检测饲料中沙门氏菌的应用研究 总被引：2，自引：0，他引：2
用聚合酶链反应(PCR)技术检测饲料中沙门氏菌,对已知被沙门氏菌污染的动物饲料培养物均能检出阳性,说明本方法对检测饲料中沙门氏菌具有特异性高、灵敏、快速等特点,适用于快速、准确地检测饲料中沙门氏菌的需要。 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2％的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。 相似文献
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