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1.
Ohe K Sakai S Sunaga F Murakami M Kiuchi A Fukuyama M Furuhata K Hara M Soma T Ishikawa Y Taneno A 《Veterinary research communications》2006,30(3):293-305
We analysed genogroups of four feline calcivirus (FCV) isolates (FCV-S, H10, Ao198-1 and ML89) obtained from cats that experienced
FCV infection after having been vaccinated against FCV. New PCR primer sets (8F/8R, Ao-S/Ao-A, cp-S/cp-A) were also designed,
since the conventional Seal primer failed to amplify the target sequences in two samples. The genogroups of the four isolates
as well as eight global and 17 domestic strains were determined by phylogenetic analysis of their amino acid sequences. One
out of the four strains (25%) isolated in this study, H10, was grouped into genogroup I, along with the vaccine strains F9
and FCV-255. The other three isolates (75%) belonged to genogroup II. Thus, there were more isolates in genogroup II than
in genogroup I. However, the antibody values of the four isolates against cat anti-F9 antisera were significantly decreased.
There may be no relationship between the neutralizing antibody titre and genogroup. Amino acid sequence alignment of the four
isolates showed that only a single amino acid in region C, which is involved in neutralization epitopes, was different in
ML89 strain from that of F9. The other three strains, H10, Ao198-1 and FCV-B, shared the same amino acid sequence with F9.
Alignment of amino acids for linear epitopes in the F9 strain, which are located at regions D and E, showed variations in
5' hypervariable region (HVR) of E, whereas D and conE had only synonymous substitutions i.e. no change in the amino acid
sequence. This mutation in 5' HVR of region E suggested a vaccine breakdown, as the region is known to be essential for antigenicity.
The genogroup II FCV is likely to be the cause of the FCV infection in this study, while the vaccine strains belong to genogroup
I. Thus, the existing vaccine may need reevaluation for its effectiveness. 相似文献
2.
The capsid protein of Australian feline calicivirus (FCV) isolates is demonstrably different from the prototype strain F9. Five Australian isolates of FCV, dating from 1970 to 1989, were analysed by western blotting and immunoprecipitation. Varying reactivity to a panel of F9 specific monoclonal antibodies (MAbs) was observed. DNA sequencing of RT-PCR generated clones supported the observation of variation between capsid proteins. Predicted amino acid sequences varied by 11 to 17.5% across the whole capsid when compared to the published F9 sequence. Differences in amino acid sequence were most apparent in previously described hypervariable regions (C and E). Within hypervariable region E differences of 22 to 34% were observed compared to F9. The observed lack of reactivity to F9 MAbs correlated with amino acid changes within previously characterized binding sites within region E. 相似文献
3.
Ohe K Sakai S Takahasi T Sunaga F Murakami M Kiuchi A Fukuyama M Furuhata K Hara M Ishikawa Y Taneno A 《Veterinary research communications》2007,31(4):497-507
Although prevention of feline calcivirus (FCV) infection by vaccination has been attempted, and isolation of FCV, development
of the disease, and a few fatal cases in vaccinated cats have been reported. Fifteen FCV strains isolated from cats that had
been vaccinated with commercially available FCV vaccines (F9, FCV-255, and FC-7) were genogrouped. Molecular analysis of viral
genomes involved the construction of a phylogenetic tree of capsid genes using the NJ method. Cat anti-F9 serum and rabbit
anti-FCV-255 serum were used for virus neutralization tests. Molecular phylogenetic analysis of the amino acid sequences of
15 virus isolates and those of the previously published and GenBank-deposited 9 global and 14 Japanese strains showed that
8 (53%) of the 15 virus isolates as well as the vaccine strains F9 and FCV-255 belonged to genogroup I (GAI), and 7 (47%) belonged to genogroup II (GAII). Of the 8 GAI strains, 2 were isolated from cats that had been vaccinated with an F9 strain live vaccine, 5 from cats vaccinated with
an FCV-255-derived vaccine, and 1 from a cat vaccinated with an FC-7-derived vaccine. Of the 7 GAII strains, 5 were isolated from cats that had been vaccinated with the F9 strain live vaccine, 1 from a cat vaccinated with
the FCV-255-derived vaccine, and 1 from a cat vaccinated with the FC-7-derived vaccine. These results indicate that more vaccine
breakdown strains isolated from the cats vaccinated with the F9 strain-derived vaccine belong to GAII than to GAI, whereas more vaccine breakdown strains isolated from the cats vaccinated with the FCV-255 strain-derived vaccine belong
to GAI than to GAII, and that when the FC-7 strain-derived vaccine is used, the vaccine breakdown strains belong almost equally to GAI and GAII. Thus, the genogroups of virus isolates varied with the vaccine strain used (p < 0.05). On the other hand, the neutralizing titres of feline anti-F9 serum and rabbit anti-FCV-255 serum against the 15
isolates were very low, showing no relationships between neutralizing antibody titres and genogroups. The DNA sequence identities
between the virus isolates and the vaccine strains were low, at 70.6–82.9%, and no strains were found to have sequences derived
from the vaccine strains. Alignment of amino acid sequences showed that the GAI or GAII virus isolates from the F9-vaccinated cats differed at position 428 of the 5’ hypervariable region (HVR) of capsid region
of the F9 strain, whereas those from the FCV-255-vaccinated cats differed at positions 438, 453, and 460 of the 5’HVR of capsid
region E of the F9 strain. We speculate that these differences influence genogrouping. The amino acid changes within the F9
linear epitopes common to G
A
I and G
A
II were noted at positions 450, 451, 457 of 5’HVR of the capsid region E in the isolates from F9-derived vaccine-treated cats,
and 449, 450, and 451 of 5’HVR of capsid region E in the isolates from FCV-255-derived vaccine-treated cats, suggesting that
these amino acid changes are involved in escapes. These results suggest that alternate vaccination with the F9 and FCV-255
strains or the use of a polyvalent vaccine containing GAII strains serves to inhibit development. 相似文献
4.
Rice CC Kruger JM Venta PJ Vilnis A Maas KA Dulin JA Maes RK 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(3):293-302
Feline caliciviruses (FCVs) are potential etiologic agents in feline idiopathic lower urinary tract disease (I-LUTD). By means of a modified virus isolation method, we examined urine obtained from 28 male and female cats with nonobstructive I-LUTD, 12 male cats with obstructive I-LUTD, and 18 clinically healthy male and female cats. All cats had been routinely vaccinated for FCV. Two FCVs were isolated; I (FCV-U1) from a female cat with nonobstructive I-LUTD, and another (FCV-U2) from a male cat with obstructive I-LUTD. To determine the genetic relationship of FCV-U1 and FCV-U2 to other FCVs. capsid protein gene RNA was reverse transcribed into cDNA, amplified, and sequenced. Multiple amino acid sequence alignments and phylogenetic trees were constructed for the entire capsid protein, hypervariable region E, and the more conserved (nonhypervariable) regions A, B, D, and F. When compared to 23 other FCV isolates with known biotypes, the overall amino acid sequence identity of the capsid protein of FCV-U1 and FCV-U2 ranged from 83 to 96%; identity of hypervariable regions C and E ranged from 58 to 85%. Phylogenetically, FCV-U1 clearly separated from other FCV strains in phenograms based on nonhypervariable regions. In contrast, FCV-U2 consistently segregated with the Urbana strain in all phenograms. Clustering of isolates by geographic origin was most apparent in phenograms based on nonhypervariable regions. No clustering of isolates by biotype was apparent in any phenograms. Our results indicate that FCV-UI and FCV-U2 are genetically distinct from other known vaccine and field strains of FCV. 相似文献
5.
Porter CJ Radford AD Gaskell RM Ryvar R Coyne KP Pinchbeck GL Dawson S 《Journal of Feline Medicine and Surgery》2008,10(1):32-40
Feline calicivirus (FCV) comprises a large number of strains which are related antigenically to varying degrees. The antigenic variability creates problems for choosing antigens to include in vaccines. Historically, these have been selected for use based on their cross-reactivity with a high proportion of field strains. However, it is important to determine the current level of cross-reactivity of vaccines and whether or not this may be decreasing owing to widespread vaccine use. In this in vitro study, we have compared the ability of antisera to two vaccine viruses (FCV strain F9 and FCV strain 255) to neutralise a panel of 40 recent UK field isolates. These 40 isolates were obtained by randomised, cross-sectional sampling of veterinary practices in different geographical regions of the UK so as to ensure they were representative of viruses circulating in the veterinary-visiting population of cats in the UK. Virus neutralisation assays showed that both vaccine strains are still broadly cross-reactive, with F9 antiserum neutralising 87.5% and 255 antiserum 75% of isolates tested with antiserum dilutions of 1 in 2 or greater. However, when antibody units were used, in order to take account of differences in homologous titres between antisera, fewer isolates were neutralised, with F9 antiserum showing a slightly higher proportion of isolates neutralised than 255. Multivariable analysis of the sample population of 1206 cats from which the 40 isolates were derived found that vaccinated cats were at a decreased risk of being positive for FCV, whereas cats from households with more than one cat, and cats with mouth ulcers were at increased risk. In addition as cats became older their risk of shedding FCV decreased. 相似文献
6.
Neutralizing feature of commercially available feline calicivirus (FCV) vaccine immune sera against FCV field isolates. 总被引：3，自引：0，他引：3
T Hohdatsu K Sato T Tajima H Koyama 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(3):299-301
Four types of commercially available feline calicivirus (FCV) vaccine were compared in terms of their efficacy on the basis of the ability of the sera of specific-pathogen-free cats immunized by two injections of each type of vaccine to neutralize FCV field isolates. Each vaccine immune serum neutralized relatively well strains F4, F9, and 255, which were FCV laboratory strains. As to 36 strains of field isolates, however, vaccines A, B, C, and D immune sera did not neutralize 18-20 of the strains (50.0%-55.6%), 19-22 of the strains (52.8%-61.1%), 22-25 of the strains (61.1%-69.4%), and 8-16 of the strains (22.2%-44.4%), respectively. These results indicate that there is much difference in neutralizing antigenicity between the existing vaccine strains and the FCV strains that are prevalent in Japan, suggesting the need for improvement of FCV vaccines. 相似文献
7.
Sung Jae Kim Cheongung Kim Hee Chun Chung Yong Ho Park Kun Taek Park 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(3)
Feline calicivirus (FCV) is a highly infectious pathogen in cats and widely distributed worldwide with high genetic variation. Full-length open reading frame 2 of 5 from recently isolated Korean FCV isolates were sequenced and compared with those of global isolates. The results of phylogenetic analysis supported dividing global FCV isolates into two genogroups (type I and II) and demonstrated the presence of genogroup II in Korea, indicating their geographic spread in East Asia. High sequence variations in region E of the FCV isolates emphasizes that a novel vaccine needs to be developed to induce protective immunity against various FCV strains. 相似文献
8.
Addie D Poulet H Golder MC McDonald M Brunet S Thibault JC Hosie MJ 《The Veterinary record》2008,163(12):355-357
This study examined a panel of 110 UK field isolates of feline calicivirus (FCV) for susceptibility to cross-neutralisation by a panel of eight antisera raised in cats infected with FCV strains F9, 255, FCVG1 and FCV431. The pairs of antisera raised against F9 or 255, neutralised 20 and 21 per cent or 37 and 56 per cent of field strains of virus respectively. In contrast, the pairs of antisera raised against the newer vaccine strains FCVG1 or FCV431 neutralised 29 and 70 per cent or 67 and 87 per cent of field strains respectively. Antisera raised against the two newer strains, namely FCVG1 and FCV431, neutralised a greater proportion of field strains of calicivirus than antisera raised against the older FCV vaccine strains F9 and 255. 相似文献
9.
东北某动物园鸽群发生异常死亡,采集病死鸽脏器,通过SPF鸡胚进行病毒分离,采用血凝试验和RT-PCR试验,鉴定结果显示尿囊液呈Ⅰ型副粘病毒核酸阳性。通过对F基因进行测序和遗传进化分析,结果表明,分离株BS0904裂解位点基序为112RRQKRF117,符合NDV强毒株的典型分子特征;分离株F基因与疫苗株LaS ota、B1、Clone 30、VG/GA、V-4、Mukteswar及经典强毒株F48E9的核苷酸同源性较低,仅为84. 2%—85. 8%;而与2011—2013年在中国多个地区鸽中所分离毒株核苷酸同源性则高达98. 8%—99. 9%,并且在遗传进化树上位于同一分支,属于ClassⅡ,基因型为Ⅵb-EU/re。该基因型毒株在中国鸽群中有较长时间、较大范围的流行传播,且与疫苗株遗传进化关系较远,说明疫苗株不能对鸽群提供有效的免疫保护,因此为了更好地对鸽群进行Ⅰ型副粘病毒的有效防控,需要针对Ⅵb基因型研发新疫苗。 相似文献
10.
为了解猫杯状病毒形态特征及遗传演化情况,采用F81细胞从患病宠物猫的鼻拭子样品中分离获得1株猫杯状病毒(feline calicivirus,FCV),命名为SH1。经电镜观察,病毒粒子呈球形,无囊膜,符合FCV的形态特征。采用RT-PCR方法扩增了该毒株的全基因组,并进行了序列测定和衣壳蛋白基因(ORF2)序列的分析。结果显示:分离株与国内外参考株的ORF2序列的核苷酸和氨基酸同源性分别为74.1%~79.7%和84.5%~90.9%;ORF2基因的遗传演化分析显示,30株FCV毒株形成两大分支,即基因群Ⅰ和Ⅱ,分离株属于基因群Ⅰ;进一步分析发现,基因群Ⅰ和Ⅱ主要在377、539和557氨基酸位点存在差异,基因群Ⅰ和Ⅱ分别为N、A、G和K、V、S。研究结果为FCV感染的防控提供科学依据。 相似文献
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12.
鹅副粘病毒F基因的克隆测序及核苷酸序列分析 总被引：18，自引：1，他引：17
以鹅副粘病毒 (GPV) SF0 2株的基因组 RNA为模板 ,通过 RT- PCR的方法扩增出 F基因片段 ,然后将其克隆至 T载体中。经 PCR鉴定后 ,对阳性克隆进行测序。测序后拼接得出 F基因的全序列。与 Gen Bank下载的 9株参考毒株比较 F基因编码区全核苷酸序列 ,发现所测 GPV- SF0 2毒株与参考毒株 L Z- NDV核苷酸序列同源性为 96 %,与F48E9同源性为 92 .5 %,与 L a Sota为 88%。 相似文献
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14.
Vlasova A Grebennikova T Zaberezhny A Greiser-Wilke I Floegel-Niesmann G Kurinnov V Aliper T Nepoklonov E 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(8):363-367
The ability to discriminate between various classical swine fever virus (CSFV) strains and isolates is a prerequisite for following the spread of the virus after an outbreak. To determine the relatedness between Russian CSFV isolates from different geographical regions, three fragments of the viral genome (5' NTR, the variable region of the E2 gene and a fragment of the NS5B gene) were sequenced and used for genetic typing. Thirty-one field isolates were obtained from CSF outbreaks which occurred between 1994 and 1999. In addition, three attenuated strains were included in the study, namely the LK and CS vaccine strains, and the moderately virulent 238H isolate. The vaccine strains have been used in Russia for more than 30 years. Our results showed that all field isolates are in subgroup 1.1 together with Alfort 187 and with the highly virulent strain Shimen. In contrast, the CS and LK vaccine strains belong to subgroup 1.2. While there is no evidence for the reversion of the two vaccine strains to wild type, it is feasible that the highly virulent Shimen strain, which has been used as a challenge strain for many years, contributed to field strain generation. The Russian field isolates from the 1990s can be distinguished from the CSF virus isolates which occurred in the EU Member States in the same decade, as here all outbreaks were caused by CSF viruses belonging to subgroup 2. 相似文献
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一株Class Ⅰ新城疫病毒中国分离株分子特性的研究 总被引：1，自引：1，他引：1
对从健康家鸭中分离到的一株Class I新城疫病毒分离株Duck/China/08-004/2008进行了遗传进化特性研究。利用RT-PCR扩增了该分离株F基因的主要功能区片段,并进行了克隆与序列分析。序列测定结果已经登录到GenBank,登录号为EU589149。该分离株F蛋白裂解位点的组成为112E-Q-Q-E-R-L117,具有典型新城疫弱毒株的分子特征。同源性分析表明其与国内普遍使用的弱毒疫苗株LaSota和V4核苷酸的同源性较低(分别为55.4%和57.7%)。通过构建F基因的遗传进化树,结果表明该分离株在分类地位上属于ClassI,与我国目前普遍使用的弱毒疫苗株LaSota(Class II中的基因II型)和V4(Class II中的基因I型)处在不同的进化分支。通过构建57株ClassI新城疫病毒的遗传进化树,表明本分离株与近年来香港活禽市场分离株较为类似,同属于基因3型。 相似文献
18.
Virus-like particles (VLPs) were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of feline calicivirus strain F9 (FCV-F9). The FCV VLPs were morphologically and antigenically similar to the native virus and contained a single capsid protein with a molecular weight of approximately 60 kDa that reacted with FCV antiserum. Moreover, following immunization of rabbits, VLPs were able to elicit neutralizing antibodies against several FCV strains isolated from clinical samples. Our preliminary results showed that FCV-VLP could be considered a candidate vaccine against FCV infections. 相似文献
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Feline calicivirus (FCV) can be typed by exploiting antigenic differences between isolates or, more recently, by the sequence analysis of a hypervariable region of the virus's capsid gene. These two methods were used to characterise FCV isolates from 20 vaccine failures which occurred after the use of a commercial, live-attenuated vaccine. Using virus neutralisation, the isolates showed a spectrum of relatedness to the vaccine; depending on the criterion adopted for identity, 10 to 40 per cent of them appeared to be similar to the vaccine virus. Using sequence analysis, the isolates fell into one of two categories; 20 per cent had a similar sequence to the vaccine (0-67 to 2-67 per cent distant), and the remainder had a dissimilar sequence (21-3 to 36-0 per cent distant). Sequence analysis identified one cat that appeared to be infected with two distinct FCVs. The serological and sequence-based typing methods gave the same result in 80 to 95 per cent of individual cases, depending on the criterion adopted for serological identity. It is suggested that molecular typing is a more definitive method for characterising the relatedness of FCV isolates. 相似文献