共查询到19条相似文献，搜索用时 218 毫秒
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裸小鼠人结肠癌术后转移模型的建立 总被引：2，自引：0，他引：2
将人结肠腺癌细胞株HCT-116接种于裸小鼠右侧背部近腋部皮下,得到皮下移植瘤,肿瘤在体内适应后用28只裸小鼠采用组织块法建立皮下移植瘤模型。4周后随机挑选15只裸小鼠作为实验组切除肿瘤组织,建立裸小鼠人结肠癌术后转移模型,其余13只裸小鼠作为对照组,观察动物生存情况及肿瘤远处转移情况。对照组裸小鼠9周时出现恶病质,解剖检查肺转移率为23%(3/13);实验组裸小鼠术部无肿瘤复发,17周后肺转移率达到100%(15/15),淋巴结转移率100%(15/15),脾脏转移率33.3%(5/15)。本模型模拟了临床肿瘤根除术后发生远处转移的过程,可为研究结肠癌转移机制和术后抗转移治疗提供理想的动物模型。 相似文献
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人癌裸小鼠原位移植瘤模型的研究及应用进展 总被引：1，自引：0，他引：1
自1969年Rygaard等首次将人类肿瘤成功地移植于探小鼠以来，大多数人类恶性肿瘤均已在棵小鼠体内建立了移植瘤模型，就其移植途径而言，多采取皮下接种方式，虽然这种移植方式简单易行，肿瘤的生长情况也便于观察，且在一定程度上也近似地模拟了原有肿瘤的形态学、生物学及生物化学特性［“j。然而，对于大多数人类恶性肿瘤来说，接种子棵小鼠皮下组织是属于异位移植，脱离J其起源组织器官的微环境，因此在实验中移植瘤的某些行为常不同于原先患者身上恶性肿瘤的生物学行为．如移植瘤常有包膜、呈局限性生长、很少发生局部或远处器官的转移… 相似文献
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通过在SCID小鼠前列腺内接种LNCaP前列腺癌细胞建立了原位接种模型并探讨其应用价值。用微量注射器在SCID小鼠的前列腺左右背外侧叶包膜下接种小鼠前列腺癌LNCaP细胞106个建立原位移植瘤模型,以皮下移植瘤模型作为对照比较两者的生存期、肿瘤转移发生情况,并在电镜下观察LNCaP前列腺肿瘤细胞和小鼠正常前列腺组织细胞的特征。结果发现原位模型组小鼠平均生存时间为(21±2.1)d较对照组(35.0±4.5)d明显缩短,差异非常显著(P0.01);原位模型组小鼠全部发生肿瘤转移,其中盆腔淋巴和肺的转移发生率分别为100%(15/15)、60%(9/15);电镜观察发现SCID小鼠的正常前列腺细胞与癌变后的细胞体积及细胞器均有很大差异。SCID小鼠的前列腺癌原位移植瘤模型较好地模拟了人癌体内的自然生长状况,为进行前列腺癌的研究提供有用的工具。 相似文献
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【目的】建立CT26细胞株BALB/c小鼠生殖器官移植肿瘤模型的方法.【方法】将CT26细胞株传代培养于小鼠皮下3代后取指数生长期细胞制成悬液,采用纤维蛋白凝胶移植接种和荷包缝合移植接种2种手术方法,移植固定于小鼠生殖器官皮下,观察小鼠生理状况、成瘤率、移植瘤成长形状和组织病理学变化.【结果】纤维蛋白凝胶移植接种成瘤率为72%,雄性雌性分组平均成瘤时间(13.37±3.06)d和(12.33±2.45)d,荷包缝合移植接种成瘤率为59%,雄性雌性组成瘤时间(15.66±3.77)d和(14.25±2.08)d,成瘤组织病理切片无明显差异.【结论】纤维蛋白凝胶移植成瘤组织病理特征符合肿瘤学动物模型需求且建模成功率高. 相似文献
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《吉林农业大学学报》2015,(5)
为比较酸奶中分离的短乳杆菌和鼠李糖乳杆菌对消化道肿瘤的潜在抑制效果,在肿瘤模型建立前2周给小鼠分别灌胃2株乳酸菌(2×108cfu),此后建立鼠结肠癌模型以比较2株乳酸菌对其是否具有抑制作用。通过给小鼠皮下注射CT26.WT细胞获得皮下增生物,经病理组织学检测为肿瘤后,通过腹部手术移植皮下瘤块至肠道表面,进而建立鼠结肠癌肿瘤模型。模型建立后通过腹部B超扫描监测肿瘤体积,并于模型建立2周后处死小鼠、分离肿瘤称重,计算抑瘤率。结果表明:皮下注射CT26.WT可以诱导获得皮下肿瘤,腹部手术、OB胶黏附瘤块建立了鼠结肠癌皮下移植瘤模型,荷瘤率为100%,术后存活率为96%以上。模型建立7 d后,腹部B超扫描检测结果表明,灌胃短乳杆菌组荷瘤鼠肿瘤的体积显著低于PBS组(P0.05),而鼠李糖乳杆菌灌胃组荷瘤鼠肿瘤体积也有下降趋势,但与PBS组相比差异不显著。分离荷瘤鼠肿瘤组织,灌胃2株乳酸菌组小鼠肿瘤重均有所下降,且短乳杆菌组更为明显,短乳杆菌抑瘤率为36.47%,鼠李糖乳杆菌抑瘤率为21.68%。构建了鼠结肠癌原位移植瘤模型,且短乳杆菌对鼠结肠癌具有较好的抑制效果,优于鼠李糖乳杆菌。 相似文献
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目的 探讨环孢菌素A(CsA)对建立皮下肺腺癌异种移植的BALB/C小鼠模型成瘤率的影响.方法 取50只BALB/C小鼠腋窝皮下接种人肺腺癌细胞系A549后,随机分为实验组和对照组各25只.实验组于接种第24h起每天腹腔注射CsA,连续10天.对照组则给予腹腔注射同体积的生理盐水.比较两组的成瘤率以及胸腺、脾脏重量指数、CD4+、CD8+T移植瘤免疫组化细胞数.结果 实验组移植成瘤率为88.00％,显著高于对照组的52.00％(P＜0.05).与对照组相比,实验组胸腺指数显著降低(P＜0.05),CD4+、CD8+T细胞数以及CD4+/CD8+T细胞比值亦显著降低(P均＜0.01),但脾脏指数差异无显著性(P＞0.05).结论 使用CsA可以提高人肺腺癌细胞系A549制备皮下异种移植的BALB/C小鼠肺癌模型的成功率. 相似文献
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Experimental therapy of human glioma by means of a genetically engineered virus mutant. 总被引：31，自引：0，他引：31
R L Martuza A Malick J M Markert K L Ruffner D M Coen 《Science (New York, N.Y.)》1991,252(5007):854-856
Malignant gliomas are the most common malignant brain tumors and are almost always fatal. A thymidine kinase-negative mutant of herpes simplex virus-1 (dlsptk) that is attenuated for neurovirulence was tested as a possible treatment for gliomas. In cell culture, dlsptk killed two long-term human glioma lines and three short-term human glioma cell populations. In nude mice with implanted subcutaneous and subrenal U87 human gliomas, intraneoplastic inoculation of dlsptk caused growth inhibition. In nude mice with intracranial U87 gliomas, intraneoplastic inoculation of dlsptk prolonged survival. Genetically engineered viruses such as dlsptk merit further evaluation as novel antineoplastic agents. 相似文献
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New method for detecting cellular transforming genes 总被引：24，自引：0，他引：24
D G Blair C S Cooper M K Oskarsson L A Eader G F Vande Woude 《Science (New York, N.Y.)》1982,218(4577):1122-1125
Tumor induction in athymic nude mice can be used to detect dominant transforming genes in cellular DNA. Mouse NIH 3T3 cells freshly transfected with either cloned Moloney sarcoma proviral DNA or cellular DNA's derived from virally transformed cells induced tumors when injected into athymic nu/nu mice. Tumors were also induced by cells transfected with DNA from two tumor-derived and one chemically transformed human cell lines. The mouse tumors induced by human cell line DNA's contained human DNA sequences, and DNA derived from these tumors was capable of inducing both tumors and foci on subsequent transfection. Tumor induction in nude mice represents a useful new method for the detection and selection of cells transformed by cellular oncogenes. 相似文献
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双歧杆菌完整肽聚糖对实验性胃癌抑制作用的研究 总被引：1，自引：0，他引：1
为研究双歧杆菌完整肽聚糖(WPG)对实验性胃癌生长的抑制作用,从两歧双歧杆菌中提取完整肽聚糖,以人胃癌裸鼠移植瘤为动物模型,观察不同浓度WPG对移植瘤生长的抑制情况,绘制肿瘤生长曲线,HE染色观察WPG对移植瘤组织形态的影响。结果显示,WPG低、中、高剂量组移植瘤的生长速度、平均瘤重均明显低于阴性对照组,抑瘤率分别为62.50%、67.87%和87.50%,与阳性对照(5-氟脲嘧啶)组相比无显著性差异(P>0.05)。HE染色结果显示,WPG组移植瘤中普遍存在不同程度的坏死。结果表明,双歧杆菌WPG能抑制裸鼠体内胃癌的生长,为开发新型高效、低毒的抗肿瘤药物提供试验依据。 相似文献
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Primary rat embryo cells transformed by one or two oncogenes show different metastatic potentials 总被引：20，自引：0，他引：20
R Pozzatti R Muschel J Williams R Padmanabhan B Howard L Liotta G Khoury 《Science (New York, N.Y.)》1986,232(4747):223-227
Second-passage rat embryo cells were transfected with a neomycin resistance gene and the activated form of the c-Ha-ras I gene, or with these two genes plus the adenovirus type 2 E1a gene. Foci of morphologically transformed cells were observed in both cases; however, the frequency of transformation was at least ten times higher with two oncogenes than with the ras gene alone. All the transformed cell lines gave rise to rapidly growing tumors when injected subcutaneously into nude mice. All but one of the cell lines transformed by the ras oncogene alone formed metastatic nodules in the lungs of animals that had been injected subcutaneously with transformed cells. When transformed cells were injected intravenously, all the ras single-gene transformants gave rise to many metastatic lung nodules. In contrast, cell lines transformed with ras and E1a did not generate metastases after subcutaneous injection and gave rise to very few metastatic lung nodules after intravenous injection. These data demonstrate that a fully malignant cell with metastatic potential, as measured in an immunodeficient animal, can be obtained from early passage embryo cells by the transfection of the ras oncogene alone. 相似文献
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Transfection of v-rasH DNA into MCF-7 human breast cancer cells bypasses dependence on estrogen for tumorigenicity 总被引：12，自引：0，他引：12
A Kasid M E Lippman A G Papageorge D R Lowy E P Gelmann 《Science (New York, N.Y.)》1985,228(4700):725-728
The natural history of estrogen-responsive breast cancers often involves a phenotypic change to an estrogen-unresponsive, more aggressive tumor. The human breast cancer cell line, MCF-7, which requires estradiol for tumor formation in vivo and shows growth stimulation in response to estradiol in vitro, is a model for hormone-responsive tumors. The v-rasH onc gene was transfected into MCF-7 cells. The cloned MCF-7ras transfectants, which expressed the v-rasH messenger RNA and v-rasH p21 protein (21,000 daltons), were characterized. In contrast to the parental cell line, MCF-7ras cells no longer responded to exogenous estrogen in culture and their growth was minimally inhibited by exogenous antiestrogens. When tested in the nude mouse, the MCF-7ras cells were fully tumorigenic in the absence of estrogen supplementation. Thus, cells acquiring an activated onc gene can bypass the hormonal regulatory signals that trigger the neoplastic growth of a human breast cancer cell line. 相似文献
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肝癌动物模型的研究及进展 总被引：1，自引：0，他引：1
[目的]建立良好的肝癌动物模型,为人类治疗肝癌服务。[方法]简单介绍几种比较常见的肝癌动物模型。[结果]目前比较常用的动物模型有：Hepa1-6细胞诱发C57BL/6J小鼠原位肝癌模型、采用Hep_G2细胞株建立裸鼠肝癌模型、直接移植法建立大鼠肝癌模型、直接注入法建立大鼠肝癌模型、免疫重建荷人高转移肝癌SCD鼠模型、其他移植性肝癌模型和基因性肝癌模型。[结论]动物模型是进行试验研究的重要手段和平台,建立肝癌动物模型对肝癌发生机制、诊断与治疗研究有很重要的意义。 相似文献
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Tumor formation dependent on proteoglycan biosynthesis 总被引：27，自引：0，他引：27
The role proteoglycans play in tumor formation was examined by measuring the tumorigenicity of proteoglycan-deficient Chinese hamster ovary cell mutants in nude mice. When 10(7) cells were injected subcutaneously, mutants with less than about 15% of the wild-type level of proteoglycan synthesis did not produce tumors. Mutants defective in the synthesis of heparan sulfate proteoglycans also did not form tumors, whereas mutants with altered chondroitin sulfate proteoglycans were tumorigenic. Tumors arose from mixtures of wild-type and nontumorigenic mutant cells and contained both cell types, suggesting that wild-type cell proteoglycans enabled mutant cells to survive. The failure of heparan sulfate-deficient mutants to form tumors depended on the ability of the host to mount a B cell-mediated immune reaction. 相似文献
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ZHANG De-Li LI Liu-jin XIA Geng-tian HE Xu-yu GAO Bu-xian BAI Xiao-hong HUANG Gao-sheng LIU Shang-gao YAN Long-Fei FANG Fu-de 《中国农业科学(英文版)》2002,1(7):816-827
The chromosomal number variations & structural aberrations of the MDCK cell line, primary feline or canine kidney cell(FKC or CKC) and Hela cell line were investigated and their karyotypes of conventional chromosome bands were analyzed. The carcinogenesis or tumorigenicity testing of these cell lines in about 232 nude mice and for colony formation in soft agarose and for haemagglutination under different concentration of plant lectins of these cells were carried out. Under the prerequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with primary feline or canine kidney cell cultures purified in vitro at passage 3 was 0 (0/22) and 0 (0/10), respectively. The incidence of the progressively-growing malignant tumor(MT) in positive-control nude mice inoculated subcutaneously with Hela cell cultures of KB, X, or NM20/X strain was 10/10, 25/25 and 5/51, respectively. The results showed that the incidence of tumor in nude mice with tetrapioid YA strain of MDCK cell during 20 - 45 passages, with hypodiploid JB strain of MDCK cell on passage 25, with di-and hypoploid JC strain of MDCK cell during 2 - 15passages or with hypoploid M strain of MDCK cell during 9 - 27 passages was 28/58, 1/5, 4/18 and 0/31,respectively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high (mcs + n) and lowest (mcs)passages was not more than 5 % - 15 % and the structure aberrations was generally 0 - 3%. These results proved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is species-specific. MDCK line has tumorigenicity no matter what its chromosome karyotype is, at least it has very low tumorigenicity even when its modal chromosome number is hypoploid. The repeatedly frozen, thawed and split controls of tumorigenicity-positive cell lines(X strain of Hela, M strain of BHK-21, JA strain of Vero, YA strain of MDCK) have much lower tumorigenicity or are even non-carcinogenesis, and the repeatedly frozen, thawed and split controls of very low tumorigenicity cell lines (M or JC strain of MDCK) are certainly non-carcinogenic and never have increased tumorigenicity.It is thus evident that MDCK cell of M, JB or JC strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YA strain can not be approved as substrate for the preparation of comattenuated viral vaccines. In summary, all strains of MDCK cell line have tunorigenicity, at least have low tumorigencity, never have non-cancinogenic MDCK, but very low tumorigenicity MDCK cell strains can certainly be used for the approval production of canine viral vaccines if the DNA content in viral cell cultures was remarkably decreased through conventional means in manufacturing process. Therefore, the master cell stock and working cell bank of MDCK line used for vaccine manufacture were established in China, which are free of infectious agents, and described with respect to cytogenetic characteristics and tumorigenicity. Tests showed that there were correlations among cell line chromosome number variations, anchorage independence in soft agarose, haemagglutination under plant lectins, and tumor-forming ability in nude mice, thus all the in vitro tests are economic, simple and reliable means for monitoring the tumor-forming ability of MDCK line in nude mice. 相似文献
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采用不完全双列杂交的方法,对4个籼型不育系和6个光身恢复系配成24个组合的株高等7个性状进行配合力分析．结果表明：光身恢复系的单株产量性状一般配合力效应为光恢1242〉光恢039〉明恢259〉光恢1240〉光恢1243〉明恢90,不育系的单株产量性状一般配合力效应为广抗13A〉钱江A〉Ⅱ-32A〉谷丰A．综合比较其他性状,光恢1242和光恢039是一般配合力效应较好的父本;广抗13A是一般配合力效应较好的母本．籼光交组合谷丰A和光恢039、钱江A和光恢1242的特殊配合力效应效应值较大,组合的单株产量竞争优势表现突出,具有较大利用价值．光身恢复系配组的约4／5组合的单株产量大于对照Ⅱ优明86． 相似文献