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1.
ＡＥＶＶａｎＲｏｅｋｅｌ鸡胚适应株在鸡胚成纤维细胞（ＣＥＦ）和鸡胚神经胶质细胞（ＣＥＢ）传代,用盲传至第６代的ＣＥＦ、ＣＥＢ制成荧光标本。建立了ＡＥＶ荧光抗体检测方法,确定了待检血清稀释度１∶１０和荧光抗体ＦＩＴＣ羊抗鸡ＩｇＧ的稀释度１∶１０的工作浓度。通过对ＮＤＶ、ＭＤＶ、ＩＢＤＶ、ＡＩＶ、Ｒｅｏ等标准阳性血清的交叉试验,证实该法特异性强,且简单、方便、经济,适合于鸡群ＡＥＶ抗体的检测。 相似文献
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应用宜兴赛尔生物化工厂产１９９、Ｆ－１０、１６４０和ＤＭＥＭ细胞培养基与美国Ｓｉｇｍａ公司产１９９、Ｆ－１０、１６４０和ＤＭＥＭ细胞培养基分别配制细胞培养液，培养ＳＰ２／０和Ｖｅｒｏ细胞系及原代鸡胚成纤维细胞（ＣＥＦ），做细胞培养动力学比较试验。比较这两种来源的细胞培养基对细胞生长的形态、分裂速度及鸡马立克氏病毒（Ｍａｒｅｋ＇ｓｄｉｓｅａｓｅｖｉｒｕｓ．ＭＤＶ）疫苗毒株ＨＶＴ－ＦＣ１２６和ＲｉｓｐｅｎｓＣＶＩ９８８在ＣＥＦ单层上增殖量的差异。研究结果表明，四种国产细胞培养基与对应的四种进口培养基对细胞生长及病毒增殖的影响无显著差异 相似文献
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鸡,鸭体内传染性法氏囊病病毒的分离及理化性质比较 总被引：2，自引：0，他引：2
从疑似传染性法氏囊病（ＩＢＤ）病鸡及同群饲养的鸭体内各分离到１株病毒，用传染性法氏囊病病毒（ＩＢＤＶ）单克隆抗体夹心ＥＬＩＳＡ试验证明两病毒均为ＩＢＤＶ，病毒血清型为Ⅰ型。病毒可致死鸡胚，适应于鸡胚成纤维细胞并产生细胞病变（ＣＰＥ）。理化性质比较表明，两病毒为同源ＩＢＤＶ。研究表明，鸭可成为ＩＢＤＶ的携带者或传染源。 相似文献
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鸭肝炎病毒A_(66)弱毒株在鸡胚成纤维细胞上的培养 总被引：4，自引：0，他引：4
将鸡胚致弱的鸭肝炎病毒（ＤＨＶ）Ａ６６株接种于鸡胚成纤维细胞（ＣＥＦ）进行细胞培养,通过对细胞培养物进行病毒滴度测定、鸡胚中和试验和电镜检查,证明了（ＤＨＶ）Ａ６６毒株能够在ＣＥＦ上增殖。试验还表明,犊牛血清对ＤＨＶ增殖具有明显的抑制作用。此外,还根据细胞培养物病毒滴度测定结果绘制出了病毒在ＣＥＦ上的生长曲线。从生长曲线可以看出,接种后８～１６ｈ病毒开始增殖,３６～６０ｈ细胞培养物中病毒滴度达最高水平。这与（ＤＨＶ）Ａ６６死鸡胚的时间基本一致鸭肝炎病毒A_(66)弱毒株在鸡胚成纤维细胞上的培养@张小飞$安徽… 相似文献
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将鸡胚致弱的鸭肝炎病毒（ＤＨＶ）Ａ６６侏接种于鸡胚成纤维细胞（ＣＥＦ）进行细胞培养,通过对细胞培养物进行病毒滴度测定、鸡胚中和试验和电镜检查,证明了ＤＨＶＡ６６毒株能够在ＣＥＦ上增殖。试验还表明,犊牛血清对ＤＨＶ增殖具有明显的抑制作用。此外,还根据细胞培养物病毒滴度毒滴度测定结果绘制出了病毒在ＣＥＦ上的生长曲线。 相似文献
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从疑似IBD病鸡的法氏囊组织中分离到1株ARV 总被引：5，自引：2，他引：3
从暴发类似传染性法氏囊病（ＩＢＤ）的江苏省某鸡场采集病鸡法氏囊组织制成组织悬液，接种鸡胚卵黄囊，部分鸡胚３～５ｄ死亡，部分鸡胚不死亡但有病变。用感染胚卵黄囊和绒尿膜混合物，接种鸡胚成纤维细胞（ＣＥＦ），盲传３代后，发现以合胞体为特征的细胞病变（ＣＰＥ）。感染细胞做超薄切片电镜观察，可见细胞浆内病毒粒子呈整齐的晶格状排列。用免疫沉淀法提取病毒抽提核酸，经ＳＤＳ－ＰＡＧＥ电泳，可见规律排列的１０条带，呈３－３－１－３排列。与禽呼肠孤病毒（ＡＲＶ）参考毒株Ｓ１１３３株的核酸谱带的数目和位置相同。血清学试验与ＡＲＶ呈阳性反应，与传染性法氏囊病病毒（ＩＢＤＶ）呈阴性反应。证明分离病毒为ＡＲＶ 相似文献
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犬瘟热,猫细小病毒,犬腺病毒弱毒株的理化特性及生物学试验 总被引：4，自引：2，他引：2
以鸡胚成纤维细胞（ＣＥＦ）、猫肾传代细胞（ＣＲＦＫ）、狗肾传代细胞（ＭＤＣＫ）从引进的疫苗中分别分离到犬瘟热ＣＤＶ－Ａ株、猫细小病毒ＦＰＶ株、犬腺病毒ＣＡＶ１株。对三个弱毒株的细胞病变规律,形态特征,病毒滴度,理化特性,核酸型,血清学交叉反应,本动物的安全性及免疫原性等内容进行了试验。鉴定结果表明,３个弱毒株均可作为制苗用毒种 相似文献
8.
本试验对鸡传染性法氏囊炎（ＩＢＤ）二价细胞苗在鸡胚成纤维细胞（ＣＥＦ）上培养所用条件进行了系统研究。对ＣＥＦ的制作，抗生素的选用，血清的浓度，接毒时间，接毒量，收获时间等进行了测定，其最佳条件为：用０．２５％的胰蛋白酶消化１０分钟制得的ＣＥＦ最好；在使用的ＰＢＳ缓冲液中加入１０００Ｕ／ｍｌ的庆大霉素可有效地防止污染；生长液用５％的小牛血清、维持液用１％的小牛血清最佳；接毒时间为生长２４小时的ＣＥＦ 相似文献
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Molecular characterization of avian polyomavirus isolated from psittacine birds based on the whole genome sequence analysis 总被引：1，自引：0，他引：1
Hiroshi Katoh Kenji Ohya Yumi Une Tsuyoshi Yamaguchi Hideto Fukushi 《Veterinary microbiology》2009,138(1-2):69-77
Seven avian polyomaviruses (APVs) were isolated from seven psittacine birds of four species. Their whole genome sequences were genetically analyzed. Comparing with the sequence of BFDV1 strain, nucleotide substitutions in the sequences of seven APV isolates were found at 63 loci and a high level of conservation of amino acid sequence in each viral protein (VP1, VP2, VP3, VP4, and t/T antigen) was predicted. An A-to-T nucleotide substitution was observed in non-control region of all seven APV sequences in comparison with BFDV1 strain. Two C-to-T nucleotide substitutions were also detected in non-coding regions of one isolate. A phylogenetic analysis of the whole genome sequences indicated that the sequences from the same species of bird were closely related. APV has been reported to have distinct tropism for cell cultures of various avian species. The present study indicated that a single amino acid substitution at position 221 in VP2 was essential for propagating in chicken embryonic fibroblast culture and this substitution was promoted by propagation on budgerigar embryonic fibroblast culture. For two isolates, three serial amino acids appeared to be deleted in VP4. However, this deletion had little effect on virus propagation. 相似文献
12.
Ha HJ Anderson IL Alley MR Springett BP Gartrell BD 《New Zealand veterinary journal》2007,55(5):235-238
AIM: To determine the prevalence of beak and feather disease virus (BFDV) infection in exotic parrots and cockatoos in the wild in New Zealand. METHODS: Eastern rosellas (Platycercus eximius, n=162) were caught from Te Puke, Wellington and Dunedin, using mistnets, between April 2004 and February 2006, and sulphur crested cockatoos (Cacatua galerita, n=255) were captured for pet-trading from November 2001 to September 2004. Feathers from both species were tested for BFDV, using an established polymerase chain reaction (PCR) test. Post-mortem examinations were conducted on some of the eastern rosellas, and selected tissues from 24 birds positive for BFDV were examined using routine histological methods for the presence of characteristic inclusion bodies. RESULTS: Of the eastern rosellas, 24/162 (14.8%) were positive for BFDV, and the 95% confidence interval (CI) for true prevalence was estimated as 8.6-20.4%, which varied between regions. Eastern rosellas that were positive for BFDV showed no clinical or histological signs of disease or inclusion bodies. Of the sulphur-crested cockatoos, 70/255 (28%) were positive for BFDV, and the 95% CI for true prevalence was calculated as 22-33%. CONCLUSIONS: The surprisingly high prevalence of BFDV in wild eastern rosellas and sulphur-crested cockatoos has serious implications for the conservation of native parrots and the export of wild-trapped parrots and cockatoos from New Zealand. Serological studies for BFDV in wild exotic parrots, and molecular studies of virus genotype, are recommended to further characterise the origin and epidemiology of the disease in populations of wild exotic parrots and cockatoos in New Zealand. 相似文献
13.
Rahaus M Wolff MH 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(8):368-371
Psittacine beak and feather disease (PBFD) is the most common viral disease of wild and captive psittacine birds. Here, we designed the first survey to investigate the existence of subclinical infections and the distribution of the causative agent named beak and feather disease virus (BFDV) inside the population of captive psittacine birds in Germany. DNA was isolated from feathers of 146 symptom-free birds from 19 different genera (all psittaformes) taken from 32 independent breeders from all over Germany. The presence of BFDV was analysed by performing polymerase chain reaction assays. Fifty-eight (39.2%) samples were found to be positive for BFDV. As expected, there was no significant predominance of one sex to be infected with BFDV. 相似文献
14.
Psittacine beak and feather disease (PBFD) is a common viral disease of wild and captive psittacine birds characterized by symmetric feather loss and beak deformities. The causative agent, beak and feather disease virus (BFDV), is a small, circular single-stranded DNA virus that belongs to the genus Circovirus. BFDV can be detected by PCR or the use of haemagglutination (HA) and haemagglutination inhibition (HI) assays that detect antigen and antibodies respectively. Erythrocytes from a limited number of psittacine species of Australian origin can be used in these tests. In South Africa, the high cost of these birds makes them difficult to obtain for experimental purposes. Investigation into the use of erythrocytes from African Grey parrots and Brown-headed parrots yielded positive results showing the haemagglutinating activity of their erythrocytes with purified BFDV obtained from confirmed clinical cases of the disease. The HA activity was further confirmed by the demonstration of HI using BFDV antiserum from three different African Grey parrots previously exposed to the virus and not showing clinical signs of the disease. 相似文献
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Breeding psittaciform birds (psittacines) from three geographically separated aviaries experiencing fledgling mortality were monitored during 1983 and 1984 for specific serum antibody to budgerigar fledgling disease virus (BFDV) using a fluorescent-antibody virus-neutralization test. Neither the time nor the extent of exposure to the virus was known. Serological titers were positive in 45% of birds sampled from Aviary 1, 25% from Aviary 2, and 11% from Aviary 3. Several species of psittacine birds within each aviary were serologically positive for BFDV. The results indicated that a papovavirus similar to BFDV appears to infect a wide range of captive adult psittacine birds. Macaws (Ara sp. and Anodorhynchus sp.) were evaluated for distribution of infection. Each species within these two genera showed positive serological titers to BFDV. Three groups of birds showed a decrease in serum antibody titer to BFDV at 1 and 2.5 months after the first sampling. Positive titers decreased from 66 to 20% for one group and from 60 to 50% for a second group in 1 month, and they decreased from 42 to 17% for a third group in 2.5 months. 相似文献
16.
Bert E Tomassone L Peccati C Navarrete MG Sola SC 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2005,52(2):64-68
Beak and feather disease (psittacine circovirus) and Budgerigar fledgling disease (avian polyomavirus) are viral diseases that can frequently affect captive psittacine birds. We designed the first survey to investigate the presence of beak and feather disease virus (BFDV) and Avian polyomavirus (APV) inside the population of captive psittacine birds in Italy. Samples were collected in 18 Italian psittacine breeding centres and four trade centres over a 4-year period. A total of 1516 birds were tested for BFDV and 877 birds were tested for APV by means of a polymerase-chain-reaction (PCR) assay. BFDV was found in 122 (8.05%) and APV in 7 (0.79%) birds. No significant difference in infection rate was found between imported and locally raised parrots. We report the first BFDV DNA isolation in wild birds imported to Italy from Papua New Guinea. 相似文献
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A universal PCR assay was designed that consistently detected psittacine beak and feather disease virus (BFDV) in psittacine birds affected with psittacine beak and feather disease (PBFD) from different geographic regions across Australia. Primers within open reading frame 1 (ORF1) of the BFDV genome consistently amplified a 717 bp product from blood and/or feathers of 32 birds with PBFD lesions. The PCR did not amplify a product from the feathers or blood from 7 clinically normal psittacine birds. Primers based on regions outside of ORF1 did not consistently produce a PCR product, suggesting there was some genomic variation outside ORF1. The amplified ORF1 PCR products of 10 BFDV isolates, from different psittacine species and from various regions around Australia, were cloned and comparative DNA sequence analysis demonstrated 88-99% of the ORF1 fragments. The derived amino acid sequences of the amplified ORF1 fragments demonstrated similar identity between all 10 isolates. Within ORF1, there was complete conservation of the putative nucleotide binding site and marked conservation of 2 other motifs previously identified as essential components of the replication-associated proteins of other circoviruses and geminiviruses. 相似文献
18.
Henriques AM Fagulha T Duarte M Ramos F Barros S Luís T Bernardino R Fevereiro M 《Avian diseases》2010,54(3):1066-1071
Beak and feather disease virus (BFDV), a member of the genus Circovirus, was detected in six dead African grey parrots (Psittacus erithacus) in Portugal. The complete nucleotide sequences of these six BFDVs (PT05, PT08, PT08-2, PT08-3, PT09, and PT09-2) were determined and analyzed. The seven open reading frames (ORFs) described for other BFDVs were detected in all strains, except for PT05 and PT08, in which ORFs 4 and 7 are absent. Bayesian inference of phylogeny based on complete genomes of BFDVs isolated in Portugal and 32 other BFDVs found in other parts of the world revealed that PT05 is included in lineage IV, whereas the others form a new proposed genotype lineage IX. The nucleotide diversity ranged from 2% to 12% between the BFDV strains isolated in Portugal and other BFDVs found worldwide. 相似文献
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应用鸡胚成纤维细胞培养鸡痘病毒 总被引：1，自引：0，他引：1
肖履中 《青海畜牧兽医杂志》2000,30(2):15-16
用丹麦产ＮＵＮＣ一次性细胞培养瓶,１９９培养基加小牛血清培养鸡胚成纤维细胞,结果显示,细胞生长快,贴壁良好,２４ｈ单层细胞即长满瓶底,无卷边脱落现象发生。将在鸡胚绒毛尿囊膜上适应了的鸡痘病毒接种鸡胚成纤维细胞,４ｄ后细胞出现预期的典型病变,并且可见大量多核巨细胞形成,证明鸡痘病毒具有诱发细胞融合的功能,而且说明,用鸡胚成纤维细胞增殖鸡痘病毒,可获得数量可观的病毒粒子。将细胞毒回归鸡胚绒毛尿囊膜,形 相似文献