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1.
《中国兽医学报》2020,(6)
为了解广东省候鸟副溶血性弧菌及其携带的毒力基因、抗生素敏感性和遗传进化特征,收集广东省候鸟粪便,用国标法和toxR基因PCR检测的方法进行分离鉴定获得副溶血性弧菌;通过PCR对毒力基因进行鉴定、用Etset测定MIC值,通过ERIC-PCR对副溶血性弧菌进行遗传进化分析。结果共获得122株副溶血性弧菌,这些细菌仅含有tlh(不耐热溶血素基因),而tdh(耐热溶血素基因)与trh(耐热溶血素相关毒素基因)等均为阴性。通过ERIC分群发现福田红树林保护区与湛江的鹭源菌株和湛江雷州的鹭源菌株具有高度相似性,深圳华侨城湿地菌株分布在3群,与雷州的鹭源相似度较高,为95%;福田红树林保护区分离株与深圳华侨城湿地菌株相似度大于75%。广东省候鸟携带的副溶血性弧菌具有较为丰富的遗传多样性,毒力基因携带单一,保持着较高的药物敏感性。 相似文献
2.
舟山沿海贝类产品中副溶血性弧菌的检测与毒力基因分析 总被引：1，自引：0，他引：1
为了解舟山沿海贝类产品中副溶血性弧菌污染情况,我们于2008年3个不同季节,从不同的农贸市场采集贝类标本,采用实时荧光定量PCR检测方法和常规培养方法,对舟山沿海贝类产品中的副溶血性弧菌进行了检测,同时对分离到的菌株进行血清学分型和毒力基因检测。结果60份贝类产品中,采用常规的分离培养方法,阳性率为83.33%,而采用实时荧光定量PCR检测方法,全部检出副溶血性弧菌。对分离到的50株副溶血性弧菌进行耐热溶血素(tdh)基因检测,结果4株tdh阳性。监测结果表明舟山贝类海产品中携带副溶血性弧菌情况比较严重,应引起足够的重视。 相似文献
3.
为了解广西部分地区(玉林市、大新县、那坡县、东兴市、靖西市和百色市)猪群中猪链球菌(Streptococcus suis,SS)毒力基因的流行情况和毒力水平,试验采用PCR方法检测分离到的201株(经鉴定猪链球菌2&1/2型24株,猪链球菌7型2株,猪链球菌9型15株,一共41株)猪链球菌的毒力基因orf2、sly、ef和mrp,并通过斑马鱼模型筛选毒力较强的菌株,测定菌株的半数致死剂量(LD50)。结果表明：从广西部分地区分离的201株猪链球菌的4种毒力基因携带率从高到低依次为orf2(85.6%,172/201)>sly(55.2%,111/201)>ef(33.8%,68/201)>mrp(26.4%,53/201);6个市县主要流行的毒力基因不同,玉林市、大新县、那坡县、东兴市、靖西市和百色市分离的猪链球菌携带最多的毒力基因分别为orf2(100%)、orf2(89.2%)、orf2(91.5%)、ef(44.4%)、orf2(86.7%)、orf2(40.0%);经鉴定分型的41株猪链球菌中,有7株(17.1%,7/41)同时携... 相似文献
4.
副溶血性弧菌（Vibrio parahaemolyticus）是海洋环境中常见的食源性致病茵。本研究从对虾中分离出1株细菌SHJLA,在TCBS和弧菌显色培养基上分别显示典型的蓝绿色和紫红色的菌落,且其生理生化特性具有典型副溶血弧菌的特性。以SHJLA菌株的基因组为模板。检测副溶血弧菌种特异性基因（tlh、toxR、groEL）均为阳性,gyrB基因序列分析表明SHJLA与副溶血弧茵的亲缘关系最近,同源性达98％～100％;检测副溶血弧菌主要毒力相关基因tdh和T3SS2（VopC2和vcrD2）均为阳性。该菌的神奈川溶血实验为阳性,对小鼠的半数致死量（LD50）为4．8×10^8 cfu／mL。结合SHJLA菌株的形态、生理生化、种特异性基因的检测、gyrB基因序列分析、毒力相关基因的检测以及小鼠半数致死量的测定结果,确定SHJLA是一株携带毒力基因的副溶血弧菌。 相似文献
5.
采用PCR方法检测辽宁锦州地区分离的150株鸡源大肠杆菌中耶尔森菌强毒力岛基因(HPI)和肠细胞脱落位点毒力岛(LEE),利用多重PCR方法检测HPI irp2和fyuA基因,以及LEE ler和eaeA基因.在分离的鸡源大肠杆菌中,HPI毒力岛基因检测结果为:18.7％的菌株irp2和fyuA基因扩增阳性,6.7％的菌株irp2基因阳性;LEE毒力岛基因检测结果为:15.3％的菌株ler和eaeA基因扩增阳性.结果表明,25.3％的鸡源大肠杆菌携带HPI,15.3％的鸡源大肠杆菌携带LEE. 相似文献
6.
《中国动物传染病学报》2015,(6)
根据副溶血弧菌种特异性基因(tox R)、耐热直接溶血素基因(tdh)和相对耐热直接溶血素基因(trh)为靶基因分别设计引物,进行PCR扩增及反应条件的优化,建立了检测含有溶血素毒力基因的致病性副溶血弧菌的多重PCR方法。3对引物能分别特异性地扩增出368、269、486 bp的目的片段。副溶血弧菌均能扩增出tox R基因,不含溶血素基因的菌株(tdh-/trh-)仅扩增出1条目的片段,而含不同溶血素基因的致病性副溶血弧菌则分别扩增出2条(tdh+/trh-或tdh-/trh+)和3条(tdh+/trh+)特异性条带。检测其他非副溶血弧菌的供试菌,则不出现任何扩增条带。人工模拟样品检测结果显示对致病性副溶血弧菌的最低检测浓度为103CFU/m L。结果表明该多重PCR检测方法具有较好的特异性和灵敏性,对检测致病性副溶血弧菌有重要意义。 相似文献
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8.
《中国兽医杂志》2014,(5)
为了获得产ESBLs和非产ESBLs菌株中毒力因子的携带情况,分析产ESBLs耐药菌株的毒力特性,为防治此类细菌性疾病提供依据。本试验采用CLSI标准方法和PCR方法分别检测青岛地区249株禽源大肠杆菌菌株的ESBLs和毒力基因。结果是83.13%的菌株产ESBLs;24种毒力基因均有检出;iutA、iroN、iss、iucA、sfa、fyuA、vat等7种毒力基因在产ESBLs菌株与非产ESBLs菌株中的检出率差异均有统计学意义。表明携带毒力因子的产ESBLs大肠杆菌菌株正在青岛地区流行和传播;毒力因子和耐药性之间具有一定相关关系,iutA、iroN、iss在产ESBLs禽源大肠杆菌中更加流行,而iucA、sfa、fyuA、vat在产ESBLs菌株中的流行率显著低于非产ESBLs菌株。 相似文献
9.
猪链球菌扁桃体分离株的毒力因子分布特征与致病性 总被引：6，自引：0，他引：6
本研究设计并合成7对引物,用PCR方法对猪链球菌7种主要毒力因子,包括谷氨酸脱氢酶(gdh)、溶血素(sly)、胞外蛋白因子(ef)、溶茵酶释放蛋白(mrp)、纤连蛋白/血纤蛋白原结合蛋白(fpbs)、三磷酸甘油醛脱氢酶(gadph)和毒力相关序列orf2进行检测,分析了29株猪链球菌扁桃体分离株的毒力因子分布特征.在被检的25株2型菌株中,共检测出7个基因型.其中10株(40%)的基因型为cps2/gdh+/sly+/ef+/mrp+/fbps+/gadph+/orf2+,7株(28%)表现为cps2/gdh+/sly-/ef-/mrp+/fbps+/gadph+/orf2+,4株(16%)表现为eps2/gdh+/sly-/ef+/mrp+/fbps+/gadph+/orl2+,基因型表现为eps2/gdh+/sly+/ef+/mrp+/fbps+/gadph+/orf2-、cps2/gdh+/sly-/ef-/mrp+/fbps+/gadph+/orf2-、eps2/gdh+/sly-/ef-/mrp*/fbps+/gadph+/orf2+、eps2/gdh+/sly-/ef-/mrp-/fbps-/gadph+/orf2-各1株;1株猪链球菌7型(SS7)分离株,基因型表现为cps7/gdh+/sly+/ef-/mrp-/fbps+/gadph+/orf2+;3株猪链球茵9型(SS9)扁桃体分离株,均表现为cps9/gdh+/sly-/ef-/mrp-/fbps+/gadph+/orf2+.可见我国SS分离株的毒力基因分布较为复杂,而且SS2的优势流行菌株是同时具有多种毒力因子的高致病茵株.通过对不同毒力基因型毒株对西藏小型猪的感染试验发现:毒力基因型为cps2/gdh+/sly-/ef+/mrp+/fbps+/gadph+/orf2+的健康扁桃体分离株SH06-21D对西藏小型猪有较强的致病性,仅次于标准株HA9801,而基因型为sly-/ef-/mrp-/fbps-/gadph+/orf2-的健康扁桃体分离株GZ06-122B对西藏小型猪不表现明显的致病性. 相似文献
10.
为了研究东北地区健康鸡源大肠杆菌毒力基因的携带分布以及PFGE分子分型情况,试验采用PCR技术对413株鸡源大肠杆菌的4种毒力基因进行检测,并运用XbaⅠ酶进行酶切后完成PFGE分析,利用软件分析菌株间的相关性和遗传关系。结果表明,在检测的413株菌株中,fimH毒力基因携带率为77.72%,iucD毒力基因的检出率为56.42%,强致病性毒力岛(HPI)的标志基因fyuA和irp2毒力基因携带率分别为44.07%和43.83%;29株大肠杆菌呈现出28种不同的PFGE型,每一株菌被XbaⅠ消化为14~20条带,菌株相似度为30%~100%;多数菌株携带毒力基因,且毒力基因的类型较为复杂,PFGE分型结果具有多样性和差异性。提示应加强鸡源大肠杆菌毒力基因的检测以及分子分型研究,为大肠杆菌病等防控提供参考。 相似文献
11.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献12.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
13.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献14.
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献17.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
18.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献19.
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
相似文献