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1.
为筛选合适狂犬病抗体检测的试剂盒，该研究以荧光抗体病毒中和试验（fluorescent antibody virus neutralization FAVN）方法为参照，与3个ELISA试剂盒方法进行比对，进行一致性分析，并计算了三种试剂盒的敏感性和特异性。结果显示：三种不同的试剂盒中，试剂盒A与FAVN一致性最好，Kappa值为0.605，有较高的一致性，符合率为86%；试剂盒B次之，Kappa值为0.485，为中度一致，符合率为80%；试剂盒C与FAVN的一致性最差，Kappa值为0.310，符合率也最低，为74%。在敏感性和特异性方面，试剂盒A的敏感性最高，为92.1%，试剂盒B的特异性最高，为72.7%。本研究为临床上科学的筛选试剂盒提供了依据。 相似文献
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高脚鸡的饲养管理要点 总被引：1，自引：0，他引：1
高脚鸡为地方品种，主要分布于贵州省普定县坪上苗族彝族布依族乡。具有跖高体大，公鸡全身羽毛红黄色，腹、翅羽呈黑色，尾羽墨绿色而有光泽，多为单冠，间有玫瑰冠，外观亮丽。母鸡羽毛多为麻黄色和黑褐色，多为单冠平头，少数为玫瑰冠，部分有胡须，喙、胫、爪多为黑色。育成母鸡的体重为1．9千克，公鸡为2．4千克。其风味独特、无腥味、味道鲜、品质优、价格高，深受消费者欢迎，市场开发前景良好。 相似文献
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蔬菜园艺是家庭园艺的重要组成部分，而发展蔬菜园艺重点依靠阳台。
阳台园艺至少有五大功能，一为观赏功能，二为实用功能，三为节流功能，四为保健功能，五为教育功能。 相似文献
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1影响母猪排卵数的因素
1．1品种 据资料报道，中国猪种的排卵数初产猪平均为17．21枚，经产猪为21．58枚，如中国嘉兴黑猪为25．68枚，二花脸为28．00枚，内江猪为15．00枚。国外猪种初产猪为13．50枚，经产猪为21．40枚，如大白猪平均为16．70枚，杜洛克为11．50枚，长白猪为15．22枚，品种不同，排卵数不同。 相似文献
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坚持以市场为导向，以科技为依托，以质量为核心，以效益为目的，以抓基地建设，典型引路为主，以点带面，适度规模，利用好现有桑树资源，以蚕业专业合作社为依托，培育小蚕专业户，推广省力化技术，搞好蚕桑综合利用，提高蚕桑综合效益。 相似文献
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胡锦涛同志2006年3月4日看望出席全国政协十届四次会议的委员时提出，要引导广大干部群众，特别是青少年树立“八荣八耻”的社会主义荣辱观。即以热爱祖国为荣、以危害祖国为耻，以服务人民为荣、以背离人民为耻，以崇尚科学为荣、以愚昧无知为耻，以辛勤劳动为荣、以好逸恶劳为耻，以团结互助为荣、以损人利己为耻．以诚实守信为荣、以见利忘义为耻，以遵纪守法为荣、以违法乱纪为耻，以艰苦奋斗为荣、以骄奢淫逸为耻。”八荣八耻“是中华传统美德和时代精神的完美结合，内容涵盖十分广泛。本文仅就有关中华传统从医美德略述如下，以供兽医界从业人员树立社会主义荣辱观参考。笔者这里所指的兽医界从业人员，不仅指临床兽医师，也包括从事兽医教学、科研、企业、行政管理等方面的从业人员。 相似文献
8.
从上海光明乳业股份有限公司车间发酵罐中分离出4株酸奶噬菌体。1号噬菌体头部呈六角形，平均直径为95.1nm，头部剖面平均面积为8184．8nm^2，尾部的平均直径为18．3nm，平均长度为403．4nm；2号噬菌体头部呈六角形，平均直径为85．4nm，头部剖面平均面积为8046．2nm^2，尾部的平均直径为23．3nm，平均长度为549．4nm；3号噬菌体头部呈六角形，平均直径为95．5nm，头部剖面平均面积为9792．3nm^2，尾部的平均直径为19．8nm，平均长度为450．5nm；4号噬菌体头部呈六角形，平均直径为119．3nm．头部剖面平均面积为12586．1nm^2，尾部的平均直径为23．6nm，平均长度为520．5nm。 相似文献
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我国奶业现状与发展对策 总被引：3，自引：0，他引：3
我国加入WTO后，发展奶业是我国畜牧业结构调整的必然选择，必须坚持以市场为导向，以效益为中心，增加科技含量，提高产品质量。不仅为加速发展奶业生产参与国际市场竞争力有着重要作用，而且为产业增效，农民增收开辟了新的致富途径。目前，奶业呈现良好的发展态势。 相似文献
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我国四川、山东、河北省蛋鸡配合饲料中镉、铬、铅污染程度评估 总被引：1，自引：0，他引：1
本试验旨在测定我国四川、山东、河北省(包括京津冀地区)蛋鸡养殖场产蛋高峰期配合饲料中镉(Cd)、铬(Cr)、铅(Pb)含量,评估蛋鸡养殖大省3种重金属污染程度。采集四川、山东、河北省67个养殖场268份蛋鸡配合饲料,采用高分辨率连续光源火焰原子吸收光谱法(HRCS FAAS)测定其Cd、Cr、Pb含量。结果表明:1)四川省蛋鸡配合饲料中Cd、Cr、Pb含量最大检出值所在城市分别为成都、乐山、乐山,最大检出值分别为0.48、15.94、14.12 mg/kg;Cr、Pb含量最高超标率所在城市分别为乐山、崇州,超标率分别为30%、50%。2)山东省蛋鸡配合饲料中Cd、Cr、Pb含量最大检出值所在城市分别为青岛和日照、菏泽、日照,最大检出值分别为0.45、5.88、4.11 mg/kg。3)河北省蛋鸡配合饲料中Cd、Cr、Pb含量最大检出值所在城市分别为张家口、张家口、保定,最大检出值分别为0.45、15.38、8.38 mg/kg;Cr、Pb含量最高超标率所在城市分别为张家口和保定,超标率均为20%。4)3省蛋鸡配合饲料中Cd、Cr、Pb平均含量分别为,四川:0.24、5.01、3.67 mg/kg;山东:0.26、4.19、2.61 mg/kg;河北:0.25、5.66、3.25 mg/kg。参照我国《饲料卫生标准》,3省蛋鸡配合饲料中Cd含量及山东省Cd、Cr、Pb含量均未超标;四川、河北省Cr、Pb含量超标率分别为9.38%、17.19%和6.73%、14.42%。5)河北省蛋鸡配合饲料中Cr、Pb平均含量显著高于山东省(P0.05);大规模养殖场配合饲料中Cd、Cr、Pb平均含量显著低于小规模养殖场(P0.05)。综上所述,山东省蛋鸡配合饲料中Cd、Cr、Pb含量均未超标。四川、河北省蛋鸡配合饲料中Cd含量未超标,但有超标风险,Cr、Pb含量均有不同程度超标,Pb含量超标最为严重,应引起重视。 相似文献
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Deborah C. Silverstein DVM DACVECC Janet Aldrich DVM Steve C. Haskins DVM MS DACVECC DACVA Kenneth J. Drobatz DVM MSCE DACVECC DACVIM Larry D. Cowgill DVM PhD DACVIM 《Journal of Veterinary Emergency and Critical Care》2005,15(3):185-192
Objective: To determine the continuous changes in blood volume in response to fluid administration using an in‐line hematocrit monitor. Design: Prospective study. Setting: Research laboratory. Animals: Four healthy dogs. Interventions: Each dog received intravenous boluses of 80 mL/kg of 0.9% saline (S), 4 mL/kg of 7.5% saline (HS), 20 mL/kg of dextran 70 (D), 20 mL/kg of hetastarch (HES), or no fluids (control, C) on separate occasions. Fluids were administered at 150 mL/min in the S, D, and HES groups, and at 1 mL/kg/min in the HS group. Measurements and main results: Blood volume changes were measured every 20 seconds for 240 minutes using an in‐line hematocrit monitor. There was a rapid rise in blood volume during all infusions. Immediately after the administration of crystalloid fluids, the rapid rise in blood volume ceased. Subsequently, there was a steep decline in blood volume for 10 minutes, and a slower decline thereafter. In contrast, the rise in blood volume continued for at least 10 minutes after the infusion of the colloids was complete, and a plateau was observed for the remainder of the experiment. The blood volume effect, as measured by area under the curve, was significantly greater in the saline group than the other groups during the infusion time and for the 0–240 minutes time period. The areas under the curve for the two colloid solutions were not significantly different from each other during any time periods. The percent increase in blood volume immediately following the infusions was 76.4±10.0 in the S group, 17.1±3.2 in the HS group, 23.0±10.5 in the D group, and 27.2±6.4 in the HES group. At 30 minutes from the start of the infusion, the mean percent increases in blood volumes were 35.2±9.3 in the S group, 12.3±0.9 in the HS group, 35.9±7.3 in the D group, and 36.8±6.5 in the HES group. At 240 h post‐infusion, the mean percent increases in blood volume were 18.0±9.7 in the S group, 2.9±6.1 in the HS group, 25.6±16.1 in the D group, and 26.6±8.6 in the HES group. The C group had a mean percent change in blood volume of ?3.7±3.4 at the end of the experiment. Conclusions: This study indicates that the rapid administration of saline at clinically relevant doses leads to the largest immediate increase in blood volume, although this change is transient because of rapid redistribution of the fluid. Despite a brief increase in blood volume that was almost 3 times the volume administered, hypertonic saline led to the smallest increase in blood volume post‐infusion. The synthetic colloid solutions increased the blood volume by an amount greater than that infused and the effect was sustained for a longer period of time than seen following crystalloid administration, but the maximum increase in blood volume was significantly less than saline. The measurement of continuous changes in blood volume, using an in‐line hematocrit monitor, was a useful means of assessing the dynamic effects of fluid administration in dogs in a research setting. 相似文献
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为了探讨吸附剂蒙脱石及营养元素铜、锌、铁、硒对肉牛体内铅、镉是否具有促排作用,试验选择40头杂交牛随机分为富硒组、微量元素组、蒙脱石组、对照组,每组10头,试验期为150 d,在试验期内每隔1个月收集1次肉牛尿液、粪便测定铅、镉含量;饲养试验结束后屠宰,采集各组织样品测定铅、镉含量。结果表明:肉牛尿液铅、镉排泄量没有明显增加;在8月份,各组粪镉含量均高于对照组(P<0.05或P<0.01);在9—11月份,与对照组相比,各组粪镉含量均无显著差异(P>0.05);在8,11月份,各组粪铅含量均无显著差异(P>0.05);在9,10月份,富硒组和微量元素组粪铅含量均低于对照组,蒙脱石组粪铅含量与对照组相比无显著差异(P>0.05)。说明在饲料中适量补充吸附剂蒙脱石,短期内对肉牛体内铅、镉有明显的促排作用;而补充营养元素铜、锌、铁、硒后,短期内在肉牛体内对镉有促排作用,对铅无促排作用,长期给予不能有效降低肉牛体内铅、镉的蓄积。提示应合理使用饲料添加剂。 相似文献
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Kazunari USHIDA Akio KISHIMOTO Shong-Ji PIAO Mitsugi ITOH Akira SHIGA Nobuo NAKANISHI Takamitsu TSUKAHARA 《Animal Science Journal》2009,80(5):556-561
Diarrhea in pigs has the potential to have a serious economic impact on the swine industry. Previously, we suggested that the likely cause of the presence of non-infectious diarrhea in pigs characterized by lactate accumulation was dyspepsia. In this experiment, the prevalence of enteropathogens and hyper-lactate accumulation in feces of piglets in 4 distinct growth stages was examined. The feces were collected when veterinarian experts recognized abnormalities in sporadic outbreaks. Prevalence of enteropathogens in diarrheal feces was 100% in fattening pigs (FP), 75% in weaning pigs (WP), 50% in suckling pigs (SP), and 42% in growing pigs (GP). Prevalence of enteropathogens in loose feces was 53% in WP, 50% in SP, 40% in FP, and 28% in GP. Prevalence of hyper-lactate accumulation in diarrheal feces was 33% in GP, 33% in SP, 25% in WP, and 25% in FP. Prevalence of hyper-lactate accumulation in loose feces was 40% in GP, 0% in SP, 7% in WP, and 5% in FP. Accordingly, non-infectious dyspepsia is frequent in growing pigs. In this period, pigs are potentially exposed to needless antimicrobial therapeutic treatments in sporadic cases. 相似文献
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本研究对规模化奶牛场产奶牛和育成牛粪中污染因子进行了四季监测,结果表明,鲜粪中含水率两类牛群夏季最高、春季最低;风干粪样中,除产奶牛夏季全氮和全磷外,产奶牛和育成牛5项检测指标及其年均值的季节变化规律均保持一致,即有机质＞全氮＞全磷＞锌＞铜;两类牛群全磷含量夏季最高、秋季最低,有机质含量春季最高、夏季最低,铜和锌含量夏季均最低;产奶牛全氮含量夏季最低、春季最高,铜含量春季最高,锌含量冬季最高;育成牛全氮含量夏季最高、秋季最低,铜含量秋季最高,锌含量春季最高. 相似文献
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体外胚胎生产作为一项高效的辅助生殖技术,对优质种质资源的保存利用及遗传改良具有重要意义。但与体内生产胚胎相比,体外生产胚胎在培养过程中出现生长发育缓慢、卵裂率低、凋亡比例增加等问题,移植后伴随着胎盘肥大、孕期延长等问题,还可能出现早产、出生体重降低、巨胎综合征(LOS)等,这与基因表达异常和表观遗传修饰异常有重要关系。文章简单回顾了近年来表观修饰学在体内、外生产胚胎的研究进展,主要介绍了印记基因的表观修饰、DNA甲基化及组蛋白乙酰化在哺乳动物体内、外生产胚胎之间的差异,简述了微阵列技术在体内、外生产胚胎之间的应用,以期找到导致体内、外胚胎质量差异的关键印记基因及其作用途径,探讨体外生产胚胎质量低于体内胚胎的原因,进而改善体外胚胎生产体系。 相似文献
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Miguel A. Perib ez Daniel F. Luco L. Garcí a Juan A. Castillo 《Preventive veterinary medicine》1997,32(3-4):287-297
The prevalence and parasite density of PKX (the unknown myxosporean that causes proliferative kidney disease [PKD] of salmonids) were investigated in eight fishfarms in Aragon, Spain. Tissue sections stained with the biotynilated lectin GS-I revealed the presence of this protozoan in only one of the farms. In rainbow trout, the renal prevalence and parasite density peaked in July, but in brown trout the maximum renal prevalence and maximum renal parasite density were reached in May and in July, respectively. In rainbow trout, after the acute phase of the disease, the number of PKX decreases in the kidney but increases in the muscle. In this species of fish, the prevalence and parasite density are much higher in the muscles than in the kidney in October. 相似文献
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驼鹿在距今约27万年的中更新世末期Piss冰期时代出现在欧洲，在距今约1万年的晚Wisconsinian时期出现在阿拉斯加，伴随着大动物区系的灭绝而出现在北美洲南部，并最终生存了下来，进而不断地进化，形成了现有的七个亚种。在近几十年的研究过程中，学术界开始更加关注分布在中国、蒙古和俄罗斯的远东驼鹿，成为研究的新热点。远东驼鹿面临着严重的狩猎威胁，存在着灭绝危险；更重要的是，这一亚种在驼鹿的进化和分类地位问题上，存在着相当大的学术争议。在以前的研究中，对于远东驼鹿的进化与分类地位的研究，主要基于形态学特征、地理分布特征以及古生物学研究来推断的，缺乏最有力的证据。但随着细胞生物学和分子生物学的发展，给驼鹿的进化和分类研究提供了更大的空间。 相似文献
19.
D. L. Doxey E. M. Milne M. P. Woodman J. S. Gilmour H. K. Chisholm 《Veterinary research communications》1995,19(6):529-543
The number of neurons in the coeliacomesenteric ganglia and the myenteric and submucosal plexuses of the jejunum, ileum and small colon, and the pathological changes induced in them, were studied in various types of equine dysautonomia. In all forms of dysautonomia, severe and extensive neuron loss and damage occurred in the ileum. In acute and subacute dysautonomia, jejunal neuron loss and damage were severe, but in chronic cases significantly less loss or damage occurred. The damage followed the same pattern in the small colon but it was always less obvious than in the jejunum. The distribution of the damage was uniform within a segment of the intestine. In fatal cases of dysautonomia, the clinical severity and duration of illness seems, in most instances, to be related to the amount of neuronal disruption occurring in the jejunum. Severe disruption results in acute/subacute dysautonomia, while milder damage leads to the chronic form.No case of dysautonomia was encountered in which enteric neuron loss and damage occurred without significant neuronal disruption also occurring in the coeliacomesenteric ganglia.Ileal neuronal damage and loss are not invariably worse than that in the jejunum, and the possible reasons for this, together with the relationship between neuronal damage and possible causes of dysautonomia, are discussed.Abbreviations H&E
haematoxylin and eosin
Deceased. Formerly of the Moredun Research Institute, 408 Gilmerton Road, Edinburgh, EH17 7JH, UK 相似文献
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为更有效地防治内蒙古自治区四子王旗地区家畜微量元素代谢病,合理使用微量元素添加剂,采用实地采样和实验室分析相结合的方法,对该地区短花针茅（Stipa breviflora）荒漠草地土壤-牧草-家畜生态系统中微量元素的水平进行检测。结果显示,土壤中铜和硒处于缺乏状态。牧草中铁和锰元素处于过量状态,硒为缺乏状态。与健康绵羊的各项指标相比较,血浆中铁和锰分别于夏秋季节和全年高于正常水平,硒和铜元素分别于秋季和冬季低于正常水平;肝脏中铁和锰全年高于正常水平,铜冬季低于临界值,硒元素秋冬季节低于正常水平;被毛中铁全年高于正常水平,而铜和硒全年低于正常水平。结合放牧绵羊对各种矿物质的进食量和消化率进行综合分析表明,该地区为高铁、高锰、低硒的生态环境,同时铜和锌处于季节性缺乏状态。 相似文献