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为探究褐菖鲉室内人工繁育技术,从野生亲鱼的收集、运输、驯化和强化培育到人工催熟催产、布苗和苗种培育,对上海地区褐菖鲉低盐度室内人工繁育模式进行了一系列探索。结果表明:2011年和2012年分别在浙江舟山地区收集野生褐菖鲉亲鱼800尾和1 200尾,经长途运输并于室内水泥池暂养48 h,分别获得存活亲鱼791尾和1 193尾,运输存活率分别为98.88%和99.42%;经3~4个月的驯化饲养,分别获得越冬前亲鱼773尾和1 158尾,驯化成活率分别为97.72%和97.07%;经越冬强化培育,分别存活728尾和1 112尾,越冬成活率分别为94.18%和96.03%。2015—2018年,经过4年室内人工繁育,通过开口生物饵料(轮虫)的培养、人工催熟催产、布苗和鱼苗培育(水体盐度17~19,水温16~18 ℃,以人工培养的海水轮虫作为开口饵料,培育周期50~60 d),分别获得体长30~40 mm的褐菖鲉幼鱼1.3万、2.1万、8.5万和26.1万尾。 相似文献
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唇(鱼骨)人工繁殖和鱼苗培育初步研究 总被引：1，自引：0，他引：1
对唇(鱼骨)(Hemibarbus labeo)的亲鱼培育、人工繁殖、孵化和鱼苗培育等技术进行了初步研究.试验结果表明:唇(鱼骨)野生亲本在池塘条件下经人工驯养可达性成熟;在水温19.0～21.0 ℃时一次性注射LHR-A2、HCG和DOM混合催产剂,其效应时间18～21.5 h;2005～2006年采用人工催产、自然产卵及人工授精、流水孵化、豆浆法培育鱼苗等方法,共催产雌鱼44尾,平均催产率81.5%,共计产卵45.7万粒,平均受精率82.6%,孵化率77.4%,共孵出鱼苗24.5万尾.鱼苗经25～30 d豆浆法培育后可达3 cm以上,平均成活率为65.5%,共培育夏花15.1万尾. 相似文献
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于2006年5月对菊黄东方鲀进行催产,催产130尾,获受精卵300万粒,孵出仔鱼160万尾,孵化率为53.3%;经39天室内培育,获平均全长25.6mm(20.1～28.4mm)苗种74万尾.成活率为46.3%. 相似文献
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双斑东方鲀全人工繁殖试验 总被引：2，自引：0，他引：2
对野生双斑东方鲀(Takifugu bimaculatus)人工繁殖的子一代进行人工培育,雌鱼须经三年性腺成熟,成熟系数为17.55%,雄鱼性腺成熟为两年。产卵前一个月,雌亲鱼经强化培育、催熟后,成熟系数可达到25.00%。对7组亲鱼注射LRH-A_2激素,共获鱼苗1.2048×10~6尾,其中全人工繁殖鱼苗9.592×10~5尾,对照组获鱼苗2.456×10~5尾。并就双斑东方鲀性成熟年龄等进行讨论。 相似文献
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对唇鱼骨(Hemibarbuslabeo)的亲鱼培育、人工繁殖、孵化和鱼苗培育等技术进行了初步研究。试验结果表明:唇鱼骨野生亲本在池塘条件下经人工驯养可达性成熟;在水温19·0~21·0℃时一次性注射LHR-A2、HCG和DOM混合催产剂,其效应时间18~21·5h;2005~2006年采用人工催产、自然产卵及人工授精、流水孵化、豆浆法培育鱼苗等方法,共催产雌鱼44尾,平均催产率81·5%,共计产卵45·7万粒,平均受精率82·6%,孵化率77·4%,共孵出鱼苗24·5万尾。鱼苗经25~30d豆浆法培育后可达3cm以上,平均成活率为65·5%,共培育夏花15·1万尾。 相似文献
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Alive eggs of marine species with pelagic eggs float, while dead eggs usually sink. A non-invasive method for counting the number of floating eggs therefore gives the possibility to track survival throughout experiments. In this paper we present an automatic image analysis method for counting live pelagic eggs of marine fish. Pelagic fish eggs are typically transparent and difficult to detect in images. Current image analysis methods for counting pelagic fish eggs are therefore done on eggs transfixed in a polymer to create contrast between the eggs and the background. This kills the eggs. The main advantage of the presented method is that it is non-invasive and only requires a minimum of handling of the eggs. As case studies we collected images of Atlantic haddock (Melanogrammus aeglefinus) and Atlantic cod (Gadus morhua) eggs. The eggs in the images were manually counted for verification of the methodology. The average counting error of false positives was 6% and the average counting error of false negatives was 2%. This demonstrates that the method is objective and accurate. 相似文献
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盐度对鮸状黄姑鱼(Nibea miichthioides)胚胎发育和仔鱼成活的影响 总被引：1，自引：0，他引：1
本研究采用不同盐度的海水对(鱼免)状黄姑鱼受精卵的沉浮性、胚胎发育的孵化时间、孵化率、仔鱼的分布状态和成活率的影响进行研究。结果表明:(鱼免)状黄姑鱼受精卵在盐度26.9‰以下的海水呈沉性,在盐度40.7‰以上的海水呈浮性,盐度26.9‰～40.7‰则由沉性向浮性过渡。(鱼免)状黄姑鱼受精卵在盐度13.7‰～53.0‰均可孵化,但孵化适合盐度为26.9‰～40.7‰。从仔鱼的分布状态、活动情况、成活率等几方面综合分析、(鱼免)状黄姑鱼仔鱼的合适盐度为26.9—33.4‰。 相似文献
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Chutima Khomvilai Masaaki Kashiwagi Chanin Sangrungruang Motoi Yoshioka 《Fisheries Science》2006,72(1):28-32
ABSTRACT: To protect chum salmon eggs from water mold infection during incubation, the eggs were treated daily with sodium hypochlorite solution (NaOCl) at 10 mg/L residual chlorine concentration for 15 min during their developmental period from fertilization to eyed stages. The number of infected eggs and number of eyed eggs were observed on day 23 of incubation. The percentage of infected eggs to total eggs was significantly lower with NaOCl treatment (1.8 : 33.4%) than in the control (11.3 : 59.3%, P < 0.01). The percentage of eyed eggs to total eggs was significantly higher with NaOCl (85.9 : 98.6%) than in the control (66.1 : 97.5%, P < 0.01). The antifungal activity of NaOCl resulted in improving egg survival. Accordingly, NaOCl is a useful antifungal agent against water mold infection on chum salmon eggs. 相似文献
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The effects of temperature and salinity on the embryonation period and hatching success of eggs of Benedenia seriolae were investigated. Temperature strongly influenced embryonation period; eggs first hatched 5 days after laying at 28 degrees C and 16 days after laying at 14 degrees C. The relationship between temperature and embryonation period is described by quadratic regression equations for time to first and last hatching. Hatching success was >70% for B. seriolae eggs incubated at temperatures from 14 to 28 degrees C. However, no B. seriolae eggs embryonated and hatched at 30 degrees C and <2% of eggs hatched when incubated at 24 degrees C after transfer to 30 degrees C for 48 h. Embryonation period was similar for eggs incubated in sea water at 25, 30 and 35 per thousand salinity, but increased for eggs incubated at higher or lower salinities. When incubated at salinities ranging from 25 to 45 per thousand, more than 70% of B. seriolae eggs embryonated and hatched. Hatching success was lower at 20 and 50 per thousand salinity and few or no eggs hatched at 10 and 15 per thousand. Hatching of B. seriolae eggs can be prevented by desiccation for 3 min, by immersion in water at 50 degrees C for 30 s or by treatment with 25% ethanol for 3 min. 相似文献
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虹鳟受精卵第一次卵裂组织学观察及四倍体制备最佳时机的研究 总被引：1，自引：0，他引：1
采用组织切片技术对虹鳟受精卵第一次卵裂进行观察,且利用热休克和静水压处理受精卵,诱导四倍体。其结果显示,静水压诱导效果较好,在受精后8h进行诱导四倍体的出现率最高,达到25～28%。热休克诱导未出现四倍体。受精卵在受精后400min(积温43.5℃.h)处于第一次卵裂前期,受精后490min(积温52.5℃.h)处于第一次卵裂中期,受精后640min(积温66.8℃.h),处于第一次卵裂后期,670min(积温69.3℃.h)处于第一次卵裂末期,700min(积温71.8℃.h)以后形成卵裂沟,并穿过整个受精卵,产生两个均等大小的两个卵裂球(blastomeres)。 相似文献
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Scanning electron microscopy indicated that stored resting eggs of the marine rotifer Brachionus plicatilis Müller were colonized by numerous bacterial cells that affect the hatchability of resting eggs. The effectiveness of chemical disinfectants on the hatching of the resting eggs was studied using selected chemicals and antibacterial drags. The hatching success rate of the fresh and stored eggs was 26 ± 5.8% and 0%, respectively. Sodium hypochlorite (1.0 p.p.m.) increased the hatching rate to 48 ± 1.3% in freshly-collected and 28 ± 1.8% in stored eggs. Physical disintegration of the bacterial mat occurred after 1 h of treatment with sodium hypochlorite. Among antibacterial drugs, sodium nifurstyrenate (Erubaju) enhanced the hatching success rate to 40 ± 1.2% and 20 ± 1.1% for the freshly-collected eggs and stored resting eggs, respectively. However, tetracycline and oxytetracycline did not significantly influence the hatching of the stored eggs. These observations are discussed in relation to the development of an improved method for the processing and hatching of rotifer resting eggs. 相似文献