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应用法氏囊弱毒疫苗GYMBOR0 PLUX对蛋鸡和肉鸡进行免疫效果试验，试验结果表明：该疫苗用于蛋鸡8～12日龄首免，间隔10d加强免疫后，可获得强毒攻击的完全保护；疫苗免疫后5d观察法氏囊变化，囊体比和囊指数在安全范围内；疫苗免疫不影响ND抗体效价滴度，对机体不产生苗源性免疫抑制用。法氏囊弱毒疫苗GUMBORO PLUX用于肉鸡，16日龄一次免疫，可获得强毒攻击的完全保护；疫苗免疫后5d观察法氏囊变化，囊体比和囊指数在安全范围内；疫苗免疫不影响ND抗体效价滴度，对机体不产生苗源性免疫抑制用。 相似文献
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鸡传染性法氏囊疫苗毒对禽脑脊髓炎油乳剂灭活苗免疫效果的影响 总被引：1，自引：0，他引：1
21日龄SPF鸡接种法氏囊中等弱毒疫苗A株后,用禽脑脊髓炎油乳剂灭活苗进行免疫,观察法氏囊疫苗毒对禽脑脊髓炎油乳剂灭活苗免疫效果的影响,用ELISA检测禽脑脊髓炎病毒抗体滴度,结果表明试验组血清抗体水平明显下降,其阳性率与对照组比较存在显著差异,说明法氏囊中等弱毒疫苗A株能造成机体免疫抑制,引起禽脑脊髓炎抗体水平下降。 相似文献
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通过观察疫苗免疫后鸡群的生长情况和临床症状,检测免疫后不同时期鸡群的ELISA抗体水平和攻毒保护率以及对鸡新城疫（ND）疫苗免疫后不同时间鸡群的HI抗体水平,评价和检测鸡传染性法氏囊病活疫苗（B87株 CA株 CF株）在石家庄地区临床应用的安全性、免疫效果、免疫持续期以及是否会产生免疫抑制。结果表明,试验疫苗接种蛋鸡和肉鸡后,均没有观察到因疫苗引起的不良反应,对蛋鸡和肉鸡均安全。疫苗接种蛋鸡后14 d、28 d、42 d、60 d和90 d,接种肉鸡后14 d、21 d、36 d攻毒保护率均达80%以上;商品蛋鸡接种疫苗后90 d其ELISA抗体水平仍高达6500以上。ND HI抗体检测结果表明,试验疫苗接种后对ND疫苗的免疫应答无显著影响,不引起免疫抑制。结论：试验疫苗安全、有效,质量稳定,适用于预防鸡传染性法氏囊病。 相似文献
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利用Laso ta系鸡新城疫病毒(NDV)接种无特定病原(SPF)鸡胚获取的含毒尿囊液,加入一定比例的矿物油佐剂制成鸡新城疫油乳剂灭活苗,和Laso ta 系弱毒疫苗联合使用免疫不同日龄不同品种的鸡群。获得了抗体滴度上升均匀在体内持续时间长,抗ND 强毒攻击的满意效果。疫苗效价高,免疫原性好,免疫保护率明显高于应用ND—Ⅰ系疫苗免疫的鸡群。对首免用Laso ta 系活苗和油乳剂灭活苗皮下注射0.1ml 的35日龄、70日龄肉仔鸡攻以ND 强毒,保护率为百分之百。对在120日龄免疫用新城疫油苗肌肉注射0.5ml 的蛋用种鸡在免疫后11个月以ND 强毒进行攻击,保护率亦为百分之百。 相似文献
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为探究ND局部免疫对蛋鸡循环抗体的影响,本研究应用鸡新城疫冻干苗COL-30、Lasota系,对不同分组的鸡群进行点眼局部免疫的效果进行比较。结果说明:不同剂量的新城疫弱毒疫苗通过点眼局部免疫后,在连续17天的循环抗体检测期间,每组的平均抗体滴度和不免疫的对照组一样,在一定的范围内(9.3log2～10.9log2)波动,即无论是Lasota还是COL-30弱毒苗,不同剂量的免疫抗体变化与同批的对照组抗体相比,相同的疫苗不同的免疫剂量抗体效价相比,差异均不显著(P>0.05)。由此可见,局部免疫并不会对原有的血液循环抗体(特别是高抗体)鸡群产生不利的影响。 相似文献
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法氏囊活性肽对新城疫弱毒疫苗免疫效果影响的研究 总被引：20，自引：0，他引：20
本研究通过鸡两次注射法氏囊活性肽后,接种新城疫弱毒疫苗,观察法氏囊活性肽对新城疫弱毒疫功免疫效果的影响,结果表明,纯化的法氏囊活性肽能够提高新城疫弱毒疫苗所诱导产生的血清抗体水平,试验组比对照线平均高１～２个滴度,使血清母源新城疫抗体水平维持较高水平,促进法氏囊的生长发育,对抗传染性法氏囊弱疫苗（ＩＢＤ）对法氏囊的损伤,和（或）促进损伤后的法氏囊的修复,对脾脏的生长发育同有影响。 相似文献
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Nadine Pa?lack Barbara Mainzer Monika Lahrssen-Wiederholt Helmut Schafft Richard Palavinskas Angele Breithaupt Jürgen Zentek 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):57-66
This study was conducted to measure the concentrations of strontium (Sr), barium (Ba), cadmium (Cd), copper (Cu), zinc (Zn), manganese (Mn), chromium (Cr), antimony (Sb), selenium (Se), and lead (Pb) in canine liver, renal cortex, and renal medulla, and the association of these concentrations with age, gender, and occurrence of chronic kidney disease (CKD). Tissues from 50 dogs were analyzed using inductively coupled plasma mass spectrometry. Cu, Zn, and Mn levels were highest in the liver followed by the renal cortex and renal medulla. The highest Sr, Cd, and Se concentrations were measured in the renal cortex while lower levels were found in the renal medulla and liver. Female dogs had higher tissue concentrations of Sr (liver and renal medulla), Cd (liver), Zn (liver and renal cortex), Cr (liver, renal cortex, and renal medulla), and Pb (liver) than male animals. Except for Mn and Sb, age-dependent variations were observed for all element concentrations in the canine tissues. Hepatic Cd and Cr concentrations were higher in dogs with CKD. In conclusion, the present results provide new knowledge about the storage of specific elements in canine liver and kidneys, and can be considered important reference data for diagnostic methods and further investigations. 相似文献
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Breed differences for weight (CW), height (CH), and condition score (CS) were estimated from records (n = 12,188) of 2- to 6-yr-old cows (n = 744) from Cycle IV of the U.S. Meat Animal Research Center's Germplasm Evaluation (GPE) Program. Cows were produced from mating Angus and Hereford dams to Angus, Hereford, Charolais, Shorthorn, Galloway, Longhorn, Nellore, Piedmontese, and Salers sires. Samples of Angus and Hereford sires were 1) reference sires born from 1962 through 1970 and 2) 1980s sires born in 1980 through 1987. The mixed model included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and breedgroup as fixed effects for CW and CS. Analyses of weight adjusted for condition score included CS as a linear covariate. The model for CH excluded PL. Random effects were additive genetic and permanent environmental effects associated with the cow. Differences among breed groups were significant (P < 0.05) for all traits and were maintained through maturity with few interchanges in ranking. The order of F1 cows for weight was as follows: Charolais (506 to 635 kg for different ages), Shorthorn and Salers, reciprocal Hereford-Angus (HA) with 1980s sires, Nellore, HA with reference sires, Galloway, Piedmontese, and Longhorn (412 to 525 kg for different ages). Order for height was as follows: Nellore (136 to 140 cm), Charolais, Shorthorn, Salers, HA with 1980s sires, Piedmontese, Longhorn, Galloway and HA with reference sires (126 to 128 cm). Hereford and Angus cows with reference sires were generally lighter than those with 1980s sires. In general, breed differences for height followed those for weight except that F1 Nellore cows were tallest, which may in part be due to Bos taurus-Bos indicus heterosis for size. 相似文献
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In experiment 1, 6 pregnant mares received a concentrate that contained a trace mineral premix that provided 14.3 mg Cu, 40 mg Zn, 28 mg Fe, 28 mg Mn, 0.08 mg Co, 0.16 mg I, and 0.16 mg Se/kg concentrate (group A). Seven mares received the same concentrate plus 502 mg Zn and 127 mg Cu once daily (group B). No differences (P > .05) in foal growth data, or Cu, Zn, and Fe concentrations of mare milk, mare serum, or foal serum were observed. In experiment 2, 6 pregnant mares received the same concentrate as group A (group C), and 8 mares received the same concentrate fortified with 4× the trace mineral premix (group D). Group C mares had higher serum Zn concentration at 1 day (P < 0.01) and 56 days (P < 0.04). Group C mares had higher milk Fe concentration at 28 days (P < .01), and group D mares had higher milk Cu concentration at 56 days (P < .01). Group C foals had higher serum Cu concentration at 14 days (P < .03). The results from this study provide no evidence to indicate that supplementing late gestating and lactating mares with higher dietary trace mineral levels than those recommended currently by NRC has any influence on foal growth and development, or on the Cu, Zn, and Fe concentrations of the mare milk, mare serum, or foal serum. 相似文献
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《饲料工业》2019,(18):54-58
应用电感耦合等离子-质谱技术(ICP-MS),建立饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅等元素的测定方法。对饲料样品的前处理方法、仪器工作参数和11种元素标准曲线进行优化;并以加标回收、分析方法比对和重复测试说明方法的准确性和精密性。方法在0～1 000 ng/ml范围内线性良好,仪器检出限为0.557 7～5.072 ng/ml,具有良好的精密度,其回收率在88.1%～104.4%之间,相对标准偏差小于5.0%。同时与原子吸收和原子荧光方法进行比对,测定结果相近。所建立的方法简单、快速,可替代原子吸收和原子荧光方法测定饲料中的11种金属元素,为饲料的质量控制提供理想的元素分析方法。 相似文献
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K Reifenberg M Stahl U L?sch 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(3):191-198
A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichment of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity, and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays. 相似文献
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《The Journal of Applied Poultry Research》2017,26(2):295-306
The present context investigated the comparative study on the supplementation of antibiotic, probiotic, organic acid, vitamin C, and herbal extract after vaccination into drinking water and their effects on performance, carcass quality, blood biochemical parameters, immune system, and intestinal flora in broiler chicks for 42 days. A total of 420 one-day-old male broiler chicks (Ross 308) were randomly assigned into 7 treatments with 3 replicates (pens) per treatment and 20 male chicks for each replicate (pen). The experimental treatments consisted of drinking water (control, without additive); drinking water + antibiotic sulfamet; drinking water + C-Vet-50; drinking water + antibiotic sulfamet + C-Vet-50; drinking water + probiotic Primalac; drinking water + butyric acid; and drinking water + extract of Echinacea purpurea Moench (coneflower). There were no differences observed among the treatments for feed intake, but during the whole experimental period, the highest body weight gain was found in the chicks fed with drinking water + antibiotic sulfamet + 50 cc vitamin C (P < 0.05). There were no differences (P > 0.05) observed among the treatments for feed conversion ratio (P > 0.05). Moreover, there were no differences reported among treatments for carcass characteristics at the end of the experiment. Among the treatments, drinking water + 50 cc vitamin C, and drinking water + extract of E. purpurea reduced (P < 0.05) the levels of cholesterol, triglycerides, and low-density lipoproteins. Drinking water + 50 cc vitamin C, drinking water + Primalac, and drinking water + extract of E. purpurea increased (P < 0.05) the lymphocytes count and decreased the heterophils count and heterophil:lymphocyte ratio. The highest Escherichia coli count and lowest Lactobacillus count in ileal content of the broilers were observed in the control group (P < 0.05). The additives used in this study may be incorporated in the drinking water of broiler chickens as growth promoters and for improved performance. A further, wider supplementation study is required to understand the performance, immune system, variation in the intestinal microbial counts, and any other possible alteration in the intestinal biota of the broilers. 相似文献
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《The Journal of small animal practice》1975,16(1-12):143-154
Circular excised skin wounds in the thoracic and metatarsal regions of the dog were studied. A similar sequence of events took place in the two regions although differences did occur due to the different reactions of the tissues which surrounded the wounds. None of the wound cavities became filled with exudate during the early stages of healing. In the thoracic wounds the cavities were largely filled by the swelling and inward movement of adipose tissue. Epithelium first grew on the wound surface in the sector of the wound that was situated in the direction of hair flow. The average time to complete epithelization was similar in both sets of wounds. A zone of alopecia developed around the wounds. 相似文献