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乳酸环丙沙星(CiprofloxacinLacticacid)为第三代喹诺酮类药物,临床应用治疗畜禽各种常见细菌和支原体感染,以及某些难治性、重症全身性感染,效果较好。笔者根据临床需要研制了乳酸环丙沙星注射液。1 仪器与材料11 仪器UV-2401分光光度计(日本岛津),HHS214型电热恒温水浴锅(山东医疗器械厂);PYX-PHS型隔水式电热恒温培养箱(上海跃进医疗器械厂);PHS2C型pH计(上海雷磁仪器厂);MA110型电子分析天平(上海天平仪器总厂)。12 药品和试剂乳酸环丙沙星(… 相似文献
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1材料与方法
1.1材料
1.1.1设备与器材:生化培养箱、高压灭菌器、放大镜、玻璃平皿等.
1.1.2试剂:营养琼脂购自杭州天和微生物试剂有限公司公司. 相似文献
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规模猪场肥育室空气细菌总数测定 总被引:1,自引:1,他引:0
1材料与方法 1.1材料 1.1.1设备与器材:生化培养箱、高压灭菌器、放大镜、玻璃平皿等。1.1.2 试剂:营养琼脂购自杭州天和微生物试剂有限公司公司。1.2方法 1.2.1培养基制备:称取营养琼脂干粉,置三角烧瓶中,加入蒸馏水,混匀后灭菌,121℃15min。将灭菌后培养基冷却至60℃左右,倒平板。 相似文献
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<正>研究以鲜黄粉虫和脱脂无糖奶粉为原料,通过乳酸菌发酵的方法,研制出一种新型黄粉虫搅拌型酸奶,为开发黄粉虫食品提供了一条新途径。1材料与仪器设备1.1材料鲜黄粉虫,河北粮丰农业科技开发有限公司提供;伊利牌脱脂无糖奶粉、优质白砂糖、光明原味桶装酸牛奶(1.1 kg)、完达山牌牛奶等,市购。1.2仪器设备电热恒温培养箱、数显恒温水浴锅、冰箱、均质机、高速组织捣碎机、电子天平、电子万用炉等,唐山职业技术学院提供。 相似文献
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利普斯E50牛奶抗生素残留检测试剂对牛奶抗生素检测具有广谱性.此试剂不仅适合乳制品生产工厂对原料及成品半成品中抗生素的检测,更适合牧场和奶站对抗生素的源头控制,从而更有效地保证了乳品安全,使乳品真正达到"无抗"要求,符合消费者需求。而在检测过程中由于各个奶站配备的恒温培养设备不同,造成有时检测结果出现有差异。本试验就是将利普斯E50牛奶抗生素残留检测试剂运用三种不同的恒温培养设备进行检测,并读取结果,以验证其在是否存在检测结果的误差,并根据试验结果来总结经验体会,同时对该试剂的情况、使用方法及注意事项进行介绍。 相似文献
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黄体的主要功能是分泌孕酮,为动物妊娠所必需。尤其对于猪,黄体是整个妊娠期孕酮的惟一来源,猪的整个妊娠期都需要有黄体的支持。妊娠期间任何时候黄体功能被破坏,都会在24~36h内导致流产[1]。笔者在查阅文献时,罕见对猪黄体细胞的研究报道,故笔者参照相关文献方法[2~9],探索了长大杂母猪黄体细胞的分离和培养方法。1材料与方法1.1样品来源所用黄体均采自附近屠宰场4~6月龄健康长大杂母猪。1.2仪器CO2培养箱;超净工作台;电子天平;电热恒温水槽;台式离心机;倒置显微镜;压力灭菌消毒器。1.3试剂胶原酶,胎牛血清,M199培养干粉,台盼蓝,HEP… 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2%的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。 相似文献
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