共查询到20条相似文献，搜索用时 187 毫秒
1.
采用酸性。α－醋酸萘醋酶（ＡＮＡＥ）反应标记淋巴细胞的细胞化学方法，石蜡连续切片，分别做ＡＮＡＥ染色和Ｈ·Ｅ、Ｗｉｌｄｅｒ法染色，对艾鼬脾脏进行组织结构及Ｔ淋巴细胞分布的研究。结果表明：艾鼬脾脏被膜较薄，被膜下淋巴组织呈弥散性分布．脾小梁不发达：白髓是以中央动脉周围淋巴鞘和脾小结的形式散布在红髓之间；牌小结未见生发中心；边缘区不发达，淋巴细胞排列疏松，在边缘区内分布有较多椭球，椭球体积较小；红髓发达，脾窦宽大。ＡＮＡＥ阳性反应淋巴细胞主要分布在中央动脉周围淋巴鞘、脾小结、边缘区和椭球内也有少量分布。 相似文献
2.
用核酸的组织化学方法对梅花鹿巴氏杆菌病脾脏Ｔ．Ｂ淋巴细胞及其ＤＮＡ和ＲＮＡ的分布进行研究。结果表明：Ｔ－淋巴细胞主要分布在中央动脉周围淋巴鞘、脾小结外周、边缘区及椭球内。Ｂ－淋巴细胞主要存在于脾小结生发中心和脾索中。ＤＮＡ和ＲＮＡ的分布未见异常，即ＤＮＡ存在于淋巴细胞核，ＲＮＡ存在于淋巴细胞的胞浆中，脾脏被膜、小梁平滑肌肿胀，排列疏松，脾髓内含有大量血液。红髓发达，脾索中浆细胞增多，窦腔内网状细胞增殖，有大量黄血盐沉着。白髓不发达，被膜下有弥散性淋巴组织，生发中心不明显；中央动脉淋巴鞘发达。边缘区不明显。鞘毛细血管数量不多，体积也比较小。 相似文献
3.
一岁龄犬淋巴器官的组织学和组织化学研究 总被引：1，自引：0，他引：1
对４只１岁龄犬的胸腺、淋巴结和脾进行了组织学和组织化学研究，并以酸性α－醋酸萘酯酶法显示Ｔ淋巴细胞的分布。结果表明：１岁龄犬胸腺发达；ＡＮＡＥ阳性淋巴细胞主要分布在胸腺小叶的皮质部；髓质内淋巴细胞数量较少，但分布有胸腺小体。淋巴结内分布有生发中心的次级淋巴小结，ＡＮＡＥ阳性反应的淋巴细胞分布在淋巴小结周围的套层及淋巴结的副皮质区。脾脏被膜和小梁发达，白髓以典型的脾小结和动脉周围淋巴鞘的形式散布在红 相似文献
4.
5.
6.
双峰驼脾脏的组织形态学观察 总被引：5，自引：0，他引：5
骆驼脾脏的形状和其它动物明显不同，为镰形，外缘呈锯齿状。其被膜很厚，富含平滑肌和弹性纤维，平滑肌层比结缔组织层厚，主要由相互垂直的两层构成。次级平滑肌性小梁发达，相互交织成网状，构成红髓的主要支架。白髓有典型的脾小结和动脉周围淋巴鞘，无边缘窦。边缘区明显，分布在脾小结、动脉周围淋巴鞘周围，可见大量吞噬含铁血黄素、ACP活性很强的巨噬细胞，开口于此的中央动脉分支十分丰富。鞘毛细血管主要分布在边缘区，其次是脾索的滤过区，白髓发达的个体更为多见，常见红细胞从椭球漏出，但胞质中未见含铁血黄素，ACP呈色反应呈阴性。脾索滤过区可见大量的毛细血管和吞噬有含铁血黄素、ACP活性很强的巨噬细胞。脾窦十分发达，窦壁细胞ACP和AKP均呈强阳性，窦腔中也有上述巨噬细胞。淋巴细胞在脾脏中的分布和其它动物及人相似。上述结果证实，骆驼脾脏属于免疫功能很强的防御性脾。 相似文献
7.
运用ＢＡ免疫组化技术与ＨＥ染色进行对比研究，探讨了实验性攻击马立克氏病病毒（ＭＤＶ）后鸡体内病毒抗原分布与病变特点之间的关系。结果表明，ＭＤＶ能引起胸腺髓质区、脾动脉周围淋巴鞘、法氏囊生发中心淋巴细胞坏死、网状细胞增生，开逐渐在全身各部位产生多发性淋巴样细胞增生灶。病毒抗原阳性反应与网状细胞增生明显相关。ＭＤＶ抗原阳性细胞主要包括网状细胞，浓缩碎裂的淋巴细胞及浸润的淋巴样细胞，这些细胞浆和胞核均哇 相似文献
8.
9.
运用ＢＡ免疫组化技术与ＨＥ染色进行对比研究，探讨了实验性攻击马立克氏病病毒（ＭＤＶ）后鸡体内病毒抗原分布与病变特点之间的关系。结果表明，ＭＤＶ能引起胸腺髓质区、脾动脉周围淋巴鞘、法氏囊生发中心淋巴细胞坏死、网状细胞增生，并逐渐在全身各部位产生多发性淋巴样细胞增生灶。病毒抗原阳性反应与网状细胞增生明显相关。ＭＤＶ抗原阳性细胞主要包括网状细胞、浓缩碎裂的淋巴细胞及浸润的淋巴样细胞。这些细胞胞浆和胞核均呈强阳性着染。 相似文献
10.
驯鹿主要周围免疫器官的组织学研究 总被引：1，自引：0，他引：1
应用HE染色法对驯鹿主要周围免疫器官－－脾及淋巴结作组织学观察，脾脏表面覆盖有浆膜，浆膜下是一层较厚的致密的结缔组织，构成被膜，被膜与实质内的脾小梁相连，实质由白髓和红髓两部分组成，白髓和红髓是相间排列，二之间的分界不明显，白髓主要由脾小结构成，脾小结由中型淋巴细胞组成，且有生发中心，脾小结中有1－2条有2－3个皱襞的中央动脉，偏于脾小结的一侧有动脉周围淋巴鞘，在白髓的外周有边缘区，边缘区内有1－2个鞘动脉。红髓由脾索和脾窦组成，脾索成索状，由网状细胞及中，小型淋巴细胞组成，脾窦不规则，其内皮细胞扁平状，核呈长梭形，脾窦内可见大量的红细胞存在。淋巴结是由被膜，实质中的皮质和髓质构成，淋巴结表面覆有薄层结缔组织被膜，皮质由淋巴小结，皮质淋已窦和副皮质区构成，髓质由髓索和髓窦构成。 相似文献
11.
Zhang W Nasu T Hosaka YZ Yasuda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(4):405-411
The distribution and population of immunocompetent cells in bovine hemal node, mesenteric lymph node and spleen were analyzed comparatively by immunohistochemistry and flow cytometry. Many CD8(+) cells, CD172a(+) cells and γδ T cells were found in the lymphatic cord along the sinus of the hemal node and the splenic red pulp. A few CD8(+) cells and γδ T cells were distributed diffusely in the paracortex and medullary cord of the mesenteric lymph node. Many germinal centers were recognized in the lymphatic regions such as the cortex and white pulp of these lymphoid organs. The populations of CD8(+) cells and γδ T cells in the hemal node and the spleen were higher than those of the mesenteric lymph node. In addition, the populations of CD21(+) cells and MHC class II(+) cells in the hemal node and the mesenteric lymph node were higher than those of the spleen. The results suggest that the hemal node has an important role in both cellular and humoral immunity as well as the lymph node and the spleen in cattle. 相似文献
12.
选取健康成年雌雄猕猴各3只,采用免疫组织化学SP法研究了雌激素受体(ER)在脾脏中的分布,观察猕猴脾脏中ER的表达及性别差异.结果显示,ER免疫阳性反应物主要分布于脾脏红髓区,脾小结相对较少,而动脉周围淋巴鞘、血管内皮、脾小粱等组织结构内仅有少量分布.ER阳性产物主要定位于细胞核中.部分存在于胞浆和胞膜上.雌性猕猴脾脏中的阳性细胞数量显著高于雄性,表达强度也较雄性强.这表明,ER参与雌激素对脾脏的免疫功能调控.ER阳性产物分布特点表明雌激素发挥作用主要是通过经典基因组机制,同时也通过非基因组机制途径.而ER表达的明显性别差异提示体内雌激素水平可能对脾脏中B淋巴细胞的功能有正调节作用. 相似文献
13.
Reona IKEGAMI Yoshimasa TANIMOTO Miori KISHIMOTO Hideshi SHIBATA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):199-202
The rabbit, which is widely used as an experimental animal and is also popular as a companion animal, has a
flat and elongated spleen with the longitudinal hilus running along its visceral surface. The spleen receives
via the hilus an arterial supply that is essential for splenic nutrition and normal functioning. However, the
distribution and variation of the arteries to the spleen have not been studied in detail. This study
investigated anatomical variations of splenic arterial supply in 33 New Zealand White rabbits with a colored
latex injection into arteries. We also examined whether the length of the spleen correlated with the number of
the splenic branches of the splenic artery. The splenic artery always arose as the first independent branch of
the celiac artery and ran along the splenic hilus to usually provide 6 (range, 3 to 10) splenic branches to
the spleen. There was a moderate correlation (R=0.6) between the number of splenic branches
and the longitudinal length of the spleen. The splenic branches often arose as a trunk or trunks in common
with short gastric arteries. The number of common trunk(s) was usually 1 (range, 0 to 4). The data showed that
the pattern and number of arterial branches to the spleen varied according to the individual animal,
suggesting that such variations should be considered when performing experimental and veterinary surgical
treatments in rabbits. 相似文献
14.
The ultrasonographic appearance, location and size of the spleen in 30 healthy female Saanen goats are described. The intercostal spaces of the left thoracic wall were scanned with a 5.0 MHz linear transducer in standing goats. The appearance of the splenic parenchyma, the position of the ultrasonographically visible dorsal and ventral margins of the spleen and the distance between them, the thickness of the spleen and the diameter of the splenic vessels were determined. The spleen could be visualised in at least one examination position and it always lay between the rumen and abdominal wall. The spleen bordered the lung dorsally and was located adjacent to the left abdominal wall in the last intercostal space and area immediately caudal to the last rib. The spleen had an echogenic capsule, and its parenchyma showed a homogenous fine echotexture / echo pattern throughout the whole visible part of the spleen. The splenic vessels were seen within the parenchyma. The visible dorsal margin of the spleen ran from cranioventral to caudodorsal. The distance from the dorsal margin of the spleen to the midline of the back was greatest in the 8th intercostal space (19.7 ± 4.19 cm) and smallest in the region immediately caudal to the last rib (7.0 ± 1.07 cm). The size of the spleen was smallest in the 8th intercostal space (3.1 ± 1.24 cm) and greatest in the 11th intercostal space (8.7 ± 2.78 cm). The thickness of the spleen ranged from 2.2 ± 1.14 cm caudal to the last rib to 6.4 ± 1.78 cm in the 11th intercostal space. 相似文献
15.
作者对３４头４～６月龄猪进行了猪丹毒的人工感染试验。试验分三组，每组又分免疫组和非免疫组。用四系强毒和自然野毒作为攻毒用菌种，静脉注射１００～５００亿活菌／头，接种３４头猪。２７头发病，死亡１５头，１～４天死亡９头；８头脾切面白髓周围出现“红晕”，出现率８８．８％；５～９天死亡猪脾切面眼观和组织学检查无“红晕”。本研究结果表明，只有病程为１～４天的特急性型死亡猪脾切面白髓切面白髓周围有“红晕”。本 相似文献
16.
刺五加多糖对雏鸡脾脏中T、B淋巴细胞定位分布的影响 总被引：1，自引：1，他引：0
为了研究雏鸡注射不同浓度刺五加多糖(ASPS)后脾脏中T、B淋巴细胞定位分布的变化情况,将60只1日龄雏鸡随机分为3组:对照组、低剂量组和高剂量组,每组20只,10日龄起对低、高剂量组雏鸡分别皮下注射免疫50和200 mg/m L的ASPS 0.2 m L,对照组雏鸡以相同方式注射等体积的生理盐水,连续注射4 d。于末次免疫后第7天和第14天,从每组中分别随机选取5只雏鸡,取其脾脏制作冰冻切片,采用免疫组织化学方法检测CD3+T淋巴细胞和Bu-1+B淋巴细胞的定位分布。结果显示,与对照组比较,低、高剂量组在注射后的7 d和14 d ASPS均能增加脾脏中CD3+T淋巴细胞和Bu-1+B淋巴细胞的数量,由CD3+T淋巴细胞构成的脾脏特征性结构-动脉周围淋巴鞘(PALS)的面积也显著增加,红髓中CD3+T淋巴细胞也明显增多,分布范围逐渐增加;同时,由Bu-1+B淋巴细胞构成的椭球周围淋巴鞘(EALS)的面积也明显增加,红髓和白髓中的浆细胞数量显著增多,并在注射后14 d时出现生发中心。与对照组比较,高剂量组和注射后14 d时上述变化趋势更为明显。结果表明,ASPS能够增加脾脏中T、B淋巴细胞的数量,并且能够影响其在PALS、EALS等脾脏特征性结构中的定位分布,从而从组织学角度进一步证明ASPS对鸡免疫功能具有显著的增强作用。 相似文献
17.
应用石蜡切片、ELISA等方法,通过对小鼠脾脏病理组织学观察以及脾脏指数、IL-2、IFN-γ和TNF-α等重要细胞因子的检测,研究猪血凝性脑脊髓炎病毒（Hemagglutinatingencephalomyelitisvirus,HEV）感染不同周龄（4、6周龄）BALB／c小鼠后的免疫动态变化特点。结果显示,无论是4周龄还是6周龄小鼠,在被HEV感染后的1～3d,脾小体生发中心增大并在红髓有多量浆细胞出现,第4天脾小体开始缩小,其周边和中央动脉周围有多量淋巴细胞聚集,到第5天脾小体生发中心消失并在其周边和中央动脉周围淋巴细胞增多。另外,脾脏指数和血清中的TNF-α、IFN-γ、IL-4浓度均呈现先升高后降低的趋势,IL-2含量变化不明显,而IL-10含量较少几乎检测不到。结果表明,HEV感染初期,小鼠对侵入的病毒做出一定的免疫应答,但是随着病毒复制,病毒量的增大,小鼠的免疫反应受到抑制。同时不同周龄小鼠的被检指标降低或升高程度及持续时间不同,表明HEV的免疫、发病与小鼠感染年龄之间存在一定相关性。 相似文献
18.
19.
AMIYA K. PATNAIK PHILIP H. LIEBERMAN† E. GREGORY MACEWEN‡ 《The Journal of small animal practice》1985,26(1):23-31
A 6-year-old male Rhodesian Ridgeback dog developed splenosis following splenectomy for a rupture of the spleen. The dog did well for 4 years following splenectomy but then developed progressive hepatomegaly, leading to the death of the animal. Grossly the abdomen was filled with masses of tissue arising from the liver. Multiple nodules of various sizes were seen in the remainder of the liver and attached to the mesentery, diaphragm and peritoneal wall. Histologically, the lesions contained splenic tissue consisting of both red and white pulp, and were characterized by all the normal cell elements of the spleen, including erythropoietic tissue. The proportion of red to white pulp was, however, abnormal, and the fibromuscular septae and sheath arteries were not obvious. In the smaller lesions, and in the margins of the large lesions, splenic tissue replaced the hepatic tissue except for the bile ducts. The multiple, intrahepatic nodules indicated splenic vein embolism. 相似文献
20.
Genna Atiee Heather Kvitko‐White Kathy Spaulding Mark Johnson 《Veterinary radiology & ultrasound》2014,55(3):310-314
Histoplasmosis is the second most common fungal infection reported in the cat. The disseminated form involving lung, liver, lymph nodes, spleen, and bone marrow is a frequent manifestation of the disease. Limited information is available in the literature regarding the ultrasonographic appearance of the spleen in cats with disseminated or splenic histoplasmosis. A retrospective review of splenic ultrasound images from 15 cats confirmed to have histoplasmosis by splenic aspirates was performed. Size, echotexture, echogenicity, margin appearance, presence of nodules, and the overall shape of the spleen were reported in each case. Splenomegaly was documented in all cases (15/15) and a hypoechoic appearance of the spleen was documented in 14/15 of cases. The spleen was diffusely and uniformly affected in 14/15 (six homogenous and eight with a subtle mottled appearance) and had discrete nodules in 1/15 cats. Histoplasmosis should be included in the differential list for an enlarged and hypoechoic spleen in cats with consistent clinical findings. Additionally, ultrasound guided splenic aspirate may be a useful method to obtain a cytology sample for diagnosis. 相似文献