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利用EST数据库资源克隆家蚕Bombyx mori6-磷酸葡萄糖酸脱氢酶基因 总被引：3，自引：1，他引：2
以果蝇6-磷酸葡萄糖酸脱氢酶(6-[jps[jpg;icpmate dehydrogenase,6PGDH)基因cDNA序列为信息探针,对家蚕EST数据进行同源检索筛选,克隆到了家蚕6-磷酸葡萄糖酸脱氢酶基因的cD-NA序列,该基因全长2011bp.经RT-PCR克隆、序列分析验证,结果表明与电子克隆序列完全一致;该基因具有完整的开放阅读框(0RF),编码蛋白为483个氨基酸;通过与人、果蝇、家鼠、摇蚊、线虫的6PGDH蛋白序列比较,发现该基因具有高度的保守性. 相似文献
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葡萄糖-6-磷酸脱氢酶(glucose-6-phosphate dehydrogenase,G6PDH,EC1.1.1.49)是磷酸戊糖途径的关键限速酶,参与NADPH和磷酸核糖的合成,在植物响应生物胁迫和非生物胁迫方面有重要作用。研究了葡萄糖-6-磷酸脱氢酶(G6PDH)在紫花苜蓿中对干旱胁迫的响应。首先采用不同浓度的PEG模拟干旱胁迫处理紫花苜蓿幼苗,通过测定不同PEG浓度下紫花苜蓿幼苗的株高、根长、干重、过氧化氢(H2O2)、丙二醛(MDA)含量的变化筛选胁迫浓度来确定干旱胁迫的条件。其次,检测胁迫浓度下紫花苜蓿幼苗中G6PDH的活性变化。第三,通过添加G6PDH的抑制剂Na3PO4,观察干旱胁迫下紫花苜蓿幼苗的生长情况并测定MDA、H2O2含量以及G6PDH活性的变化。最后,综合分析G6PDH对干旱胁迫的响应。结果显示,不同浓度PEG处理下,紫花苜蓿幼苗的生长受到限制,例如株高、根长、鲜重、干重都随PEG浓度的增加而逐渐减小,MDA和H2O2的含量随PEG浓度的增加而增加,其中以15% PEG处理的影响最大;同时,也发现随PEG浓度的增加紫花苜蓿幼苗中G6PDH的活性也较对照增加,15%PEG处理时达最高值;故15%的PEG处理为干旱胁迫模拟最佳条件。通过添加G6PDH的抑制剂Na3PO4后发现,干旱胁迫下紫花苜蓿幼苗的生长明显地受到抑制,其叶片中MDA、H2O2含量分别较干旱胁迫下的增长28.4%、19.9%,G6PDH的活性也明显降低了49.4%。以上结果初步说明G6PDH可能参与调节了干旱胁迫引起的氧化胁迫。 相似文献
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采用聚丙烯酰胺凝胶垂直板电泳(PAGE)技术,对11株Ei meria maxima和1株E.tenella的孢子化卵囊,进行了乳酸脱氢酶(LDH)、葡萄糖磷酸异构酶(GPI)、葡萄糖-6-磷酸脱氢酶(G6PD)、葡萄糖磷酸变位酶(PGM)和苹果酸脱氢酶(MDH)的同工酶分析。试验结果显示,E.maxima和E.tenella在LDH、GPI、G6PD、PGM和MDH的酶谱上有明显差异,而在11株E.maxima之间无差异,表明E.maxima的酶变异相当保守。 相似文献
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致死基因是一个连锁基因织的一部分,该连锁基因组包括血型位点S和H、红细胞酶位点、磷酸己糖异构酶(phi)和6-磷酸葡萄糖酸脱氢酶(pgd)及原生质α_1B-蛋白糖原位点。知道了由一个或多个这样的连锁群构成的表现型,就有可能预测出致死基因型。据报道,魁北克长白猪,致死基因与phi-B和Pgd-B表型有关。它们可以作为强烈致死的血液标记。 相似文献
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为研究6-磷酸山梨醇脱氢酶基因在乳酸菌中超表达的影响,本试验根据副干酪乳杆菌的基因序列设计引物,用PCR的方法扩增6-磷酸山梨醇脱氢酶(gutF)基因,将其连接到乳酸菌表达载体pMG36e,并将重组质粒转化到副干酪乳杆菌及乳酸乳球菌中获得重组菌株;对重组菌株表达产物进行蛋白质水平的电泳检测,检测出表达了大约28kD的蛋白;使用山梨醇替代培养基中的葡萄糖,以未转化基因菌株做阴性对照,进行生长曲线的测定,结果表明,转化菌株能够利用在以山梨醇作为碳源的条件下,重组菌株的生长速度和最终菌浓度均优越于对照菌株。本研究通过超表达6-磷酸山梨醇脱氢酶蛋白,对于进一步研究乳酸菌的耐受性具有十分重要的理论基础。 相似文献
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柔嫩艾美耳球虫孢子发育三种阶段的同工酶研究 总被引：2，自引：0，他引：2
采用聚丙烯酰胺凝胶电泳(PAGE)研究柔嫩艾美耳球虫(Eimeria tenella)未孢子化卵囊,孢子化卵囊、孢子囊的乳酸脱氢酶(LDH)、葡萄糖-6-磷酸脱氢酶(G6PD)、异柠檬酸脱氢酶(IDH)、6-磷酸萄萄糖酸脱氢酶(6PGD)、磷酸葡萄糖变位酶(PGM)、碱性磷酸酶(ALP)和葡萄糖磷酸异构酶(GPI)等7种酶的同工酶,除IDH外,6种酶均显示出初步结果,孢子化卵囊和孢子囊6种酶的同工酶完全一致,未孢子化卵囊的LDH、G6PD、PGM、ALP、GPI 5种酶同工酶与孢子化卵囊、孢子囊一致,未孢子化卵囊的6PGD同工酶与孢子化卵囊、孢子囊呈现明显差异、讨论了球虫在孢子化过程中,一些酶的活性或同工酶发生变化的情况。 相似文献
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五种鸡球虫卵囊的同工酶研究 总被引：3，自引：0，他引：3
采用聚丙烯酰胺凝胶管状电泳，研究柔嫩艾美耳球虫、毒害艾美耳球虫、巨型艾美耳球虫、变位艾美耳球虫和堆型艾美耳球虫卵囊的乳酸脱氢酶、葡萄糖磷酸异构酶、葡萄糖－６－磷酸脱氢酶、碱性磷酸酶、６－磷酸葡萄糖酸脱氢酶的同工酶。结果显示５种球虫卵囊的５种同工酶酶谱能反映出虫种差异。作者认为同工酶技术有助于球虫种的分类。 相似文献
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Detection of linkage between genetic markers and genes that affect growth and carcass traits in pigs. 总被引：2，自引：0，他引：2
P A Clamp J E Beever R L Fernando D G McLaren L B Schook 《Journal of animal science》1992,70(9):2695-2706
Segregation of paternal marker alleles in the progeny of a single boar was used to estimate linkage between the marker genes and associations of these genes with quantitative trait loci (QTL). The sire was heterozygous at four polymorphic marker loci, haptoglobin (HP), glucosephosphate isomerase (GPI), phosphogluconate dehydrogenase (PGD), and esterase D (ESD), and sired 30 litters during an 8-mo period. Glucosephosphate isomerase and PGD were linked (theta = .09; P less than .005). The phase of these two loci in the sire was determined to be GPI A-PGD B, GPI B - PGD A. NO other linkages were detected. Growth (135 less than or equal to n less than or equal to 172) and carcass data (70 less than or equal to n less than or equal to 80) were analyzed assuming a fixed linear model. Least squares means were compared for differences in growth and carcass traits between pigs that inherited alternative paternal marker alleles. Pigs that inherited the GPI A allele from the sire had a 22-g higher daily live weight gain postweaning and reached 103 kg live weight in 2.6 fewer days than did pigs that inherited the GPI B allele (P less than .05), indicative of the presence of gene(s) that affect rate of gain linked to the GPI locus. Pigs that inherited the PGD B allele had a .14 unit higher score for muscle firmness (score ranged from 1 to 3 units) than pigs that inherited the PGD A allele (P less than .05). Pigs that inherited the HP 3 allele had a .06-kg higher weaning weight and a .11 lower ham muscle mass score than did pigs that inherited the HP 2 allele from the sire (P less than .05). No associations with quantitative traits were detected for ESD. 相似文献
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三河马运铁蛋白型的研究 总被引：4，自引：0，他引：4
采用聚丙烯酰胺凝胶电泳对 2 5匹三河马血清样品进行了分析。发现 1 2个运铁蛋白表现型 ,即DD ,F2 F2 ,RR ,DF2 ,DH2 ,DR ,F1 R ,F2 H2 ,F2 O ,F2 R ,H2 R和OR。其中F2 F2 ,DF2 和OR所占的比例最高 ,分别为 32 % ,1 6 %和 1 2 % ,其他表现型的比例都小于 8%。运铁蛋白型由 6个共显性常染色体等位基因控制 ,即TfD,TfF1 ,TfF2 ,TfH2 ,TfO 和TfR,其基因频率分别为 0 1 6,0 0 2 ,0 48,0 0 6 ,0 1 0和 0 1 8。基因杂合度 (H)和亲权排除概率 (PE)分别为 0 70和 0 47。 相似文献
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中国矮马血红蛋白多态性的初步研究 总被引：4，自引：0，他引：4
采用等电聚焦电泳对中国矮马进行了血红蛋白多态性的检测。结果表明 :在该位点共发现有 2种基因型 ,由 2个等位基因HbBⅡ 、HbBⅠ 控制。基因型BⅡ /BⅡ、BⅠ /BⅡ的频率分别为 0 62 5 0、 0 375 0 ,其中纯合型BⅡ /BⅡ为优势基因型。基因频率HbBⅡ 、HbBⅠ 分别为 0 81 2 5、0 1 875。其基因杂合度 (H)、个体鉴别概率 (Dp)和亲仔关系排除概率 (PE)分别为 0 30 4 7、 0 4690和 0 1 2 90。 相似文献
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中国矮马血液遗传标记的初步研究 总被引：3，自引：0，他引：3
采用聚丙烯酰胺凝胶电泳对16匹中国矮马血清中白蛋白（ALB）、酯酶（ES）、α1-B糖蛋白（A1B）和维生素D结合蛋白（GC）进行了检测。结果发现：在4个位点中，白蛋白（ALB）和酯酶（ES）位点呈现多态性，而α1-B糖蛋白（A1B）、维生素D结合蛋白（GC）2个位点均呈现单态性。在白蛋白（ALB）位点发现3个基因型，即AA、AB和BB，由等位基因ALB^A和ALB^B控制，其基因频率分别为0.3125和0.6875；在酯酶（ES）位点发现4个基因型，即FS、II、FF和FI，由等位基因ES^s、ES^1和Es^f控制，其基因频率分别为0.1875,0.281和0.5313。 相似文献
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Nyström PE Juneja RK Johansson K Andersson-Eklund L Andersson K 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》1997,114(1-6):363-368
SUMMARY: The effect of the genotypes of five different blood protein loci (α1B-glycoprotein, A1BG; glucose phosphate isomerase, GPI; phosphogluconate dehydrogenase, PGD; postalbumin 1A, PO1A; transferrin, TF) on early body-weight traits was studied in one large population of Swedish Yorkshire breed pigs. A highly significant association was observed, between the transferrin genotypes and the piglet body weights, at 6 and 9 weeks of age. The TF BB type pigs were heavier than those of TF AB types at 3, 6, and 9 weeks of age, by 130, 340, and 370 g, respectively. In the light of previously published data, it was discussed that TF is an additional chromosome 13 marker that may affect early body weights in pigs. The other four loci studied, located on chromosomes 6 and 7, did not show any significant effect. ZUSAMMENFASSUNG: Zusammenh?nge zwischen Transferrinlocus an Chromosom 13 und Ferkelgewichten Die Wirkung von fünf verschiedenen Blutproteinloci (αB-Glykoprotein, A1BG; Glukose Phosphat Isomerase, GPI; Phosphoglukonat Dehydrogenase, PGD; Postalbumin 1A, PO1A; Transferrin, TF) auf Ferkelwichte wurde bei Schwedischen Yorkshire Schweinen untersucht. Der Zusammenhang zwischen Transferrin Genotypen und 6 und 9 Wochen Gewichten war hochsignifikant, TF BB Ferkel waren bei 3, 6 und 9 Wochen Alter um 130, 340 und 370 g schwerer als TF AB Ferkel. In zusammenhang mit früheren Studien wird TF als ein weiterer Chromosom 13 Marker für Ferkelgewicht er?rtert. Die anderen vier Loci an Chromosomen 6 bzw. 7 zeigten keine signifikante Wirkung. 相似文献
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Suto J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(4):449-456
The objectives of this study were to characterize plasma lipid phenotypes and dissect the genetic basis of plasma lipid levels in an obese DDD.Cg-A(y) mouse strain. Plasma triglyceride (TG) levels were significantly higher in the DDD.Cg-A(y) strain than in the B6.Cg-A(y) strain. In contrast, plasma total-cholesterol (CHO) levels did not substantially differ between the two strains. As a rule, the A(y) allele significantly increased TG levels, but did not increase CHO levels. Quantitative trait locus (QTL) analyses for plasma TG and CHO levels were performed in two types of F(2) female mice [F(2)A(y) (F(2) mice carrying the A(y) allele) and F(2) non- A(y) mice (F(2) mice without the A(y) allele)] produced by crossing C57BL/6J females and DDD.Cg-A(y) males. Single QTL scan identified one significant QTL for TG levels on chromosome 1, and two significant QTLs for CHO levels on chromosomes 1 and 8. When the marker nearest to the QTL on chromosome 1 was used as covariates, four additional significant QTLs for CHO levels were identified on chromosomes 5, 6, and 17 (two loci). In contrast, consideration of the agouti locus genotype as covariates did not detect additional QTLs. DDD.Cg-A(y) showed a low CHO level, although it had Apoa2(b), which was a CHO-increasing allele at the Apoa2 locus. This may have been partly due to the presence of multiple QTLs, which were associated with decreased CHO levels, on chromosome 8. 相似文献
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Streptococcus suis type 2 is a pathogen responsible for diverse diseases in both pigs and humans. In order to understand the pathogenesis of the S. suis type 2 infection, the gene encoding a cell surface protein, 6-phosphogluconate-dehydrogenase (6PGD) of S. suis type 2 was cloned and sequenced, and recombinant 6PGD protein (r6PGD) was produced in a prokaryotic expression system. Sequence analysis of the cloned 6 pdg gene showed 82% similarity with Streptococcus pneumoniae 6 pdg at the nucleic acid level. Western blotting using r6PGD-specific antiserum confirmed the cell surface location of the 6PGD protein of S. suis type 2. The role of 6PGD in S. suis type 2 pathogenesis as an adhesin and its immunogenicity in mice was further investigated. The results showed that the recombinant protein interfered with the adhesion of S. suis type 2 to Hep2 and HeLa cells by 72% and 66%, respectively. Immunization of CD-1 mice with r6PGD increased the protective efficacy by 80% following intraperitoneal administration of a lethal dose of S. suis type 2. Immunization of CD-1 mice with r6PGD elicited a significant protective immune response, which demonstrated the importance of 6PGD to bacterial pathogenesis. Identification and characterization of the role of S. suis type 2 6PGD in adhesion and immunogenicity will allow us to use this protein to develop new antimicrobial therapies and/or vaccines. 相似文献
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Suto J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(8):1003-1006
Growth deficit (gd) is a recessive mutation that occurs spontaneously in the inbred NC/Sgn mouse strain. Because homozygotes (gd/gd) of both sexes are sterile, they must be produced by mating putative heterozygous carriers (+/gd) whose phenotypes are essentially the same as those of wild-type +/+ mice. The objectives of this study were to develop an efficient method that distinguished a gd allele from a wild-type allele and, if possible, to identify nucleotide substitutions responsible for the gd mutation. The location of the gd locus was estimated to be at 58.3 Mbp on chromosome 4, over which Musk is located. An A-to-G base substitution, which resulted in an M826V amino acid exchange, was identified within a tyrosine kinase domain of Musk. This base substitution disrupted a recognition site for NlaIII; this allowed for discriminating the gd allele from the wild-type allele using PCR-RFLP analysis. When 130 (C57BL/6J × NC/Sgn-gd) F(2) mice were genotyped by PCR-RFLP analysis, all 32 growth-retarded F(2) mice were judged to have the gd/gd genotype. Musk mutations are known to cause congenital myasthenia, which is accompanied by growth retardation, postnatal lethality, and development of a hunchback. These were the typical phenotypes of gd/gd mutants. Although we cannot rule out the possibility that the neighboring genes around the Musk locus are related to the gd phenotype, gd could possibly be classified as a mutant allele of Musk. 相似文献