首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 31 毫秒
1.
为了观察绵羊用多头蚴抗原免疫及感染后的抗体消长规律,为羊脑多头蚴病的免疫预防和免疫诊断提供依据,本试验应用多头蚴原头节可溶性抗原、囊壁可溶性抗原、囊液粗抗原致敏绵羊红细胞对绵羊免疫3次及虫卵攻击感染后的血清抗体进行间接血凝试验（IHA）检测。结果表明,原头节抗原免疫组、囊壁抗原免疫组、囊液抗原免疫组及原头节ES抗原免疫组在首次免疫后1周,抗体滴度迅速升高,第3次免疫后1周达到峰值,虫卵感染后开始下降,到感染后30周接近正常水平。多头蚴3种抗原对同种抗原免疫组血清检测敏感性、特异性优于其它抗原,原头节免疫组、囊壁免疫组、囊液免疫组抗体水平明显高于原头节ES抗原免疫组。  相似文献   

2.
许玮  杨倩 《中国兽医学报》2012,32(4):552-555,569
通过禽流感灭活抗原配合黏膜免疫佐剂鼻腔免疫乳鸽,研究鸽呼吸道各段抗体分泌细胞的分布和数量。结果显示,首免后第3、5周,应用CpG免疫后肺IgG分泌细胞面积显著高于对照组（P〈0.05）,首免后第5,7周,应用CpG免疫后肺IgA分泌细胞面积显著高于对照组（P〈0.05）;首免后第3、5、7周,应用灭活禽流感抗原免疫后,鸽呼吸道各部位IgG分泌细胞和IgA分泌细胞面积与对照组无显著差异;应用禽流感抗原配合CpG和胆酸钠免疫后鸽呼吸道各部位单位面积中IgA分泌细胞和IgG分泌细胞面积均显著或极显著高于对照组（P〈0.01,P〈0.05）。结果表明,灭活禽流感抗原配合黏膜免疫佐剂通过鼻腔免疫能够提高呼吸道中IgA分泌细胞和IgG分泌细胞的面积,增强局部呼吸道体液免疫应答水平。  相似文献   

3.
The serum antibody titers to diphtheria toxoid and human serum albumin were determined in 103 goat kids from lines selected for 12 yr for high or low antibody response to diphtheria toxoid. In the 12th yr, six groups of kids were immunized with different preparations of the antigens. In all groups but one, the antigens were emulsified in Freund's incomplete adjuvant with added sonicated Mycobacterium paratuberculosis. The groups received the following treatments: Group 1 was immunized with both antigens mixed in the same syringe, Group 2 got both antigens injected separately, Group 3 got both antigens injected separately, but with a lower concentration of M. paratuberculosis, Group 4 was immunized with diphtheria toxoid only, Group 5 was immunized with human serum albumin only, and Group 6 was immunized with both antigens mixed, but without any M. paratuberculosis. The animals were immunized at 4 wk of age, and the antibody titers were determined 3 wk later by ELISA and passive hemagglutination. The mean antibody titers to both antigens were different between the selected lines (P less than .03). There was no effect of separate vs combined injections of antigens. However, there were indications of antigen suppression or competition between the antigens. Animals receiving only one antigen seemed to mount a higher antibody response to that antigen than did animals immunized with two antigens.  相似文献   

4.
1-day-old SPF chickens and commercial Jingfen chickens were vaccinated with IBD immune complex(IC) vaccine, NDV La Sota vaccine were inoculated simultaneously every one week and every two weeks.NDV La Sota immunization alone was as the control group.At the 2nd, 3rd, 4th and 5th week post inoculation, blood samples were taken and the ND HI antibody were tested.Experimental chickens were challenged with high virulent NDV at the 5th week post inoculation, the protective rate of each group was calculated.The results showed the ND HI antibody were not significant different in the combined immunization of IBD IC vaccine priming and NDV La Sota vaccine boost and NDV La Sota vaccine alone immunization (P>0.05).The results indicated that IBD IC vaccine has no immunosuppression on NDV La Sota vaccine in SPF chickens and commercial Jingfen chickens.  相似文献   

5.
The ability of young pigs to be immunized during the postnatal period was studied. Eight groups of pigs born on the same day from 3 sows were injected with hen egg-white lysozyme in Freund's incomplete adjuvant at days 0, 1, 2, 3, 4, 5, 7, and 10 after birth. The serum antibody titers were determined each week. Results indicated that pigs injected within the first 3 days of life exhibited a delay of 10 days in the appearance of the humoral antibodies, compared with the antibody response observed in pigs injected later. Serum antibody titers were markedly lower in the early immunized pigs. The secondary immune response was similar in all pigs. This partial inhibition is not directly linked to the corticoids present in the serum at the immunization day. Possible reasons for this impairment of the humoral immune response were reviewed.  相似文献   

6.
Antigenic sharing among the most commonly bacterial pathogens such as Aeromonas hydrophila, Edwardsiella tarda and Pseudomonas fluorescens of Indian major carps has been studied using immunological reactions such as cross-agglutination, disc diffusion and indirect enzyme linked immunosorbent assay (ELISA). The data were analysed using statistical analysis (SAS), version 6.12. The results showed high antigenic similarities among the bacterial whole cells, whole cell lysates, somatic 'O' antigens, lipopolysaccharides (LPS) and extracellular products (ECP). However, few or no similarities were observed in ECP components of <20kD. The present study indicates a need to develop differential diagnostic methods based on serology by choosing the highly specific less cross-reactive ECP antigen.  相似文献   

7.
将90只大鼠随机分为5组,分别注射10μg.100μL-1 pcISI(T1)、50μg.100μL-1 pcISI(T2)、100μg.100μL-1(T3)pcISI、100μg空载体pcMV-S(V)和100μL生理盐水(S),以探讨在不使用免疫佐剂的情况下,不同剂量的双拷贝抑制素pcISI基因免疫对大鼠卵泡发育、产仔和生殖激素的影响。结果表明,加强免疫能提高抗体P/N值,加强免疫后各剂量组平均抗体P/N值大于2,T3组抗体P/N值显著高于T1和T2组(P<0.05)。T3组成熟卵泡数显著高于对照组(P<0.05),且抗体阳性鼠成熟卵泡数比阴性鼠多12.45个(P<0.05)。除了T1组外,其他2个剂量组产仔数和胎盘数均高于对照组(P<0.05)。抗体阳性鼠胎盘数比阴性鼠多5.09个(P<0.05),产仔数比阴性鼠多5.39个(P<0.05)。抑制素pcISI基因免疫大鼠后促卵泡素(FSH)、雌二醇(E2)和孕酮(P4)含量均高于对照组,T3组的FSH含量与对照组差异显著(P<0.05),T3组的E2含量与对照组差异极显著(P<0.01),各剂量组P4含量与对照组差异均不显著(P>0.05)。这些结果说明,在没有免疫佐剂的前提下,双拷贝抑制素pcISI基因免疫可产生抗体,促进FSH分泌和卵泡发育,增加产仔数。本试验条件下100μg.100μL-1是最佳免疫剂量。  相似文献   

8.
Twenty-eight dogs immunized to dinitrophenol-ascaris (DNP-ASC) at birth and then three times at two week intervals produced serum anti-DNP antibody. The IgM response was detected one week after primary immunization and lasted for up to five weeks. The IgE and IgG antibody response in general was not present until week three but persisted through the immunization schedule. Although variation in the level and duration of the antibody response was detected between individual dogs, each dog did have a response that included all three isotypes examined.  相似文献   

9.
试验旨在探究生长抑素（SS）和皮质抑素（CST）双表达DNA疫苗免疫杂交水牛育成牛的促生长效果,并对其安全性进行验证。将鉴定正确的pVGS/2SS-2A-S/CST-asd质粒电转入减毒猪霍乱沙门氏菌C500构建生长抑素和皮质抑素双表达活载体疫苗。将16头6~12月龄杂交水牛育成牛随机分为低（TL）、中（TM）、高（TH）剂量组及PBS对照组（NC）,每组4头,试验组和对照组分别用双表达疫苗和PBS进行鼻腔免疫,初免时每天09：00和15：00免疫2次,连续免疫3 d,2周后以相同程序加强免疫1次,对免疫后不同时期的增重效果和免疫应答水平等进行监测。免疫应答结果显示,各剂量组免疫后均引起了免疫应答,TL组在初免后2周产生的SS抗体水平高于TM和TH组,而TM组SS抗体阳性率最高,为100%,优于TL （75%）和TH组（50%）;初免后2周产生的CST抗体水平则呈现完全相反的趋势,抗体水平随免疫剂量下降呈下降的趋势;初免后7周SS抗体和CST抗体水平和阳性率整体呈下降趋势,但也有部分牛出现抗体升高趋势;免疫因子检测结果显示,初免后2周TL和TM组IL-4水平极显著高于对照组（P<0.01）,而免疫后7周对照组的IL-4水平显著高于TL组（P<0.05）,与其他各组之间差异均不显著（P>0.05）;对照组INF-γ水平在初免后2周显著高于TL和TM组（P<0.05）,免疫后7周对照组显著高于TL组（P<0.05）,与其他各组之间差异均不显著（P>0.05）;对比抗体阳性组和阴性组发现,初免后2周抗体阳性组的IL-4水平极显著高于阴性组（P<0.01）,初免后7周则显著低于对照组（P<0.05）,而抗体阳性组INF-γ在初免后2和7周均显著低于阴性组（P<0.05）。增重效果检测显示,初免后2周各试验组平均日增重均显著高于对照组（P<0.05）,而在其他各时期各组之间差异均不显著（P>0.05）,抗体阳性组仅在初免后2周显著高于阴性组（P<0.05）。激素检测结果显示,各试验组生长激素（GH）和胰岛素样生长因子-1（IGF-1）在初免后2周均极显著高于对照组（P<0.01）,而初免后7周TL组GH和IGF-1水平显著低于对照组（P<0.05）,其他各组之间均差异不显著（P>0.05）;抗体阳性组的GH和IGF-1水平在初免后2周极显著高于阴性组（P<0.01）,而初免后7周则极显著低于阴性组（P<0.01）。安全性检测结果显示,各时期采集的水样、土样、粪样中均未检测到目的片段,证实了疫苗的安全性。本试验结果表明,双表达疫苗免疫后机体能产生良好的免疫应答,能提高育成牛的平均日增重,且对环境未产生安全性问题。  相似文献   

10.
将山羊随机分为6组，即E／SA纽、E／SA＋cpG组（未乳化）、E／SA＋CpG组、E／SA＋IL-2组、E／SA＋IL-2＋CpG组、对照组，每组3只，分别于免疫前、免疫后第2周、免疫后第4周、感染后第1周及感染后第2周采集山羊外周全血及涂血片，IHA法检测抗体滴度的动态变化；ELISA法检测IFN-γ、TNF—a、IL-2、IL-4的表达水平；ANAE染色法检测T淋巴细胞的动态变化。结果显示，免疫后各免疫组的IFN-γ、TNF-a、IL-2、IL-4及T淋巴细胞水平显著高于对照组（P〈0．05），各免疫组的抗体水平均高于对照组。结果表明，E／SA可引起IFN—γ、TNF—a、IL-2、IL-4、T淋巴细胞及抗体水平的升高，说明E／SA免疫后可引起山羊较强的细胞免疫和体液免疫应答。  相似文献   

11.
选用4株带有K88、K99、987P、F41粘附素抗原的产肠毒素性大肠杆菌,分别接种于BBL、Minca、Slanetz和Minca培养基进行培养,将培养物加热提取粘附素抗原后,加入油佐剂制成四价亚单位疫苗。经成品检验合格后分别免疫小鼠和怀孕母猪,同时监测怀孕母猪抗体。最后1次免疫后15 d,分别用同源ETEC确定的攻毒剂量攻击。结果显示,经2次免疫后,K88、K99、987P、F41对小鼠的免疫保护率与1次免疫没有显著差异(P>0.05);对仔猪,1次免疫跟2次免疫均可显著地降低腹泻指数(P<0.05),2次免疫与1次免疫没有显著差异(P>0.05)。怀孕母猪免疫1周后(产前23 d)抗体开始上升,第2周(产前16 d)达到高峰。2次免疫后抗体迅速回升,第4周(产前2 d)达到最高峰,产后2 d抗体大幅度下跌,几近免疫前水平。二免母猪所产仔猪发病率明显低于一免母猪(P<0.05)。攻毒保护试验和抗体消长规律的结果表明,制备的仔猪大肠杆菌病K88-K99-987P-F41四价亚单位疫苗具有良好的免疫原性,能有效预防仔猪大肠杆菌病的发生。  相似文献   

12.
应用间接荧光抗体法检测鱼类嗜水气单胞菌的试验研究   总被引：6，自引：0，他引：6  
作者制备了鱼类嗜水气单胞菌（AH）－77株兔抗血清,建立了应用间接荧光抗体试验（IFA）检测鱼类嗜水气单胞菌病原的方法。该法能有效地检测人工感染鳟鱼体内的嗜水气单胞菌病原体,对杀鲑气单胞菌、荧光假单胞菌、爱德华氏菌不发生交叉反应。整个过程可在2．5h内完成,是一种有前途的鱼类嗜水气单胞菌快速诊断方法。  相似文献   

13.
This study aimed to assess systemic and mucosal immune responses of Atlantic salmon (Salmo salar) exposed to two protein-hapten antigens – dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) – administered using different delivery strategies. Fish were exposed to the antigens through different routes, and were given a booster 4 weeks post initial exposure. Both systemic and mucosal antibody responses were measured for a period of 12 weeks using an enzyme-linked immunosorbent assay (ELISA). Only fish exposed to both antigens via intraperitoneal (IP) injection showed increased systemic antibody response starting 6 weeks post immunization. No treatment was able to produce a mucosal antibody response; however there was an increase in antibody levels in the tissue supernatant from skin explants obtained 12 weeks post immunization from fish injected with FITC. Western blots probed with serum and culture supernatant from skin explants showed a specific response against the antigens. In conclusion, IP injection of hapten-antigen in Atlantic salmon was the best delivery route for inducing an antibody response against these antigens in this species. Even though IP injection did not induce an increase in antibody levels in the skin mucus, there was an increased systemic antibody response and an apparent increase of antibody production in mucosal tissues as demonstrated by the increased level of specific antibody levels in supernatants from the tissue explants.  相似文献   

14.
小鼠对温和气单胞菌口服缓释微球疫苗的免疫应答   总被引：2，自引：1，他引：1  
采用中华鳖温和气单胞菌(Aeromonas sobria,As)Z-1株灭活全菌液,以生物降解性高分子材料为载体,制成微球疫苗,口服免疫ICR小鼠,测定了血清中凝集抗体效价、血液中单核-巨噬细胞的吞噬活性以及对活菌攻击的免疫保护力.结果显示小鼠微球疫苗口服免疫后第4周,血清中凝集抗体效价和血液中单核-巨噬细胞的吞噬指数均可达到与灭活菌液注射组相当的水平(P＞0.05),明显高于灭活菌液口服组(P＜0.01);免疫后第7～12周,微球疫苗口服组血清凝集抗体效价显著高于灭活菌液注射组(P＜0.05);微球疫苗口服组和灭活菌液注射组的免疫保护力均为87.5%,而灭活菌液口服组不能提供有效的保护,与对照组死亡率相同(100%).结论认为,中华鳖气单胞菌口服微球疫苗具有缓释作用;以可生物降解的微球作为中华鳖气单胞菌口服疫苗的载体系统具有潜在的优势.  相似文献   

15.
The aim of this study was to investigate if immunization with the ferri-siderophore receptors FepA, FhuE, IroN and IutA could protect chickens against avian pathogenic Escherichia coli (APEC) infection. The antigens were administered as recombinant proteins in the outer membrane (OM) of E. coli strain BL21 Star DE3. In a first immunization experiment, live E. coli expressing all 4 recombinant ferri-siderophore receptors (BL21(L)) were given intranasally. In a second immunization experiment, a mixture of E. coli ghosts containing recombinant FepA and IutA and ghosts containing recombinant FhuE and IroN was evaluated. For both experiments non-recombinant counterparts of the tentative vaccines were administered as placebo. At the time of challenge, the IgG antibody response for BL21(L) and a mixture of E. coli ghosts containing recombinant FepA and IutA and ghosts containing recombinant FhuE and IroN was significantly higher in all immunized groups as compared to the negative control groups (LB or PBS) confirming successful immunization. Although neither of the tentative vaccines could prevent lesions and mortality upon APEC infection, immunization with bacterial ghosts resulted in a decrease in mortality from 50% (PBS) to 31% (non-recombinant ghosts) or 20% (recombinant ghosts) and these differences were not found to be significant.  相似文献   

16.
Cholera (and related) toxins (CT) when applied topically on unbroken skin induce systemic immune responses in mice, a procedure called transcutaneous immunization (TCI). The current study examined the capacity for TCI to induce systemic immune responses in sheep. Three groups (n=5 per group) were immunized at day 0 (priming) and day 28 (boosting) with 250 microg of CT in water by TCI, with 25 microg of CT in alum by intramuscular injection, or not immunized. Serum samples were taken at days 0, 28, 42, 56 and 70 after immunization for measurement of CT-specific IgG as well as CT-specific IgG1, IgG2, IgA and IgM antibodies by ELISA. After immunization, IgG, IgG1 and IgG2 antibody in immunized groups were significantly higher than in the control group, and boosting further increased these titres. IgG, IgG1 and IgG2 in the injection group were significantly higher than in the TCI group. There was a preponderance of IgG1 antibody, relative to IgG2, in both immunized groups. CT-specific IgA and IgM were detected in both immunized groups. Lymphocyte proliferation to CT was measured at day 90. A CT-specific lymphocyte proliferative response (stimulation index>2) was detected in all sheep from the injection group, in two sheep from the TCI group and in none of the controls. Results demonstrated that TCI induces primary and secondary antibody responses and specific proliferative responses to CT in sheep.  相似文献   

17.
An enzyme-linked immunosorbent assay (ELISA) using antibody to affinity-purified Oreochromis aureus immunoglobulin and antigens from the parasitic dinoflagellate amyloodinium ocellatum was developed. The ELISA was then used to evaluate the immune response of the tilapine fish to immunization with the parasite. Fish immunized with antigens of the dinospore stage, either live or sonicated, produced a specific immune response that was detectable by this ELISA. Combinations of serial dilutions of A. ocellatum antigen and fish anti-A. ocellatum serum were examined to determine which dilutions provided optimal differentiation of seropositive from seronegative fish. Fresh and heat-inactivated serum from both seropositive and seronegative fish produced similar results.  相似文献   

18.
魏氏梭菌类毒素疫苗研制及其免疫家兔抗体消长规律   总被引：4，自引：0，他引：4  
本研究分别从山东省的莱芜、泰安、潍坊的三个养兔场发生疑似梭菌性下痢的病兔体分离到三株魏氏梭菌，鉴定为A型。利用该分离菌株和A型魏氏梭菌标准株（CVCC37）所产外毒素经甲醛灭活并加入氢氧化铝胶佐剂吸附浓缩后制备了A型魏氏梭菌类毒素疫苗。对该疫苗分别进行了安全性检验、有效免疫剂量试验和接种家兔1～23周血清中抗毒素（Ab）消长规律研究。结果表明，魏氏梭菌外毒素用03％甲醛32h能够彻底灭活，并最大可能地保持其抗原性；制备的魏氏梭菌类毒素疫苗无毒副作用，安全可靠；用野毒株和标准株制备的类毒素疫苗对家兔的有效免疫剂量为2mL／只，免疫保护效果可靠；采用野毒株和标准株制备的类毒素疫苗接种家兔后第二周血清抗毒素效价迅速升高，到第四周达最高峰。分别为6．6一和7．125log2，较高抗体滴度维持约17周后缓慢下降，至23周时血清平均抗毒素滴度仍维持在4．0-和3．4log2以上，所以，该类毒素疫苗的免疫保护期可以设定为6个月。  相似文献   

19.
This study was designed to evaluate the synergistic effect of VA5 immunopotentiator on pigeon ND4416 inactivated vaccine.160 healthy pigeons were randomly divided into four groups,including ND4416 strain inactivated vaccine group (NDV group),ND4416 strain inactivated vaccine and immunopotentiator (VA5) mixed group (NDV+VA5 group),La Sota inactivated vaccine group (La Sota group) and normal saline as the control group (C group),to assess their vaccine efficacy against virulent pigeon NDV by serological analysis and animal testing.Pigeons sera were collected at different time points after immunization and measured the HI antibody titer of each group.The results showed that VA5 immunopotentiator significantly improved the serum antibody level of pigeons immunized with pigeon Newcastle disease inactivated vaccine (P<0.05).In addition,comparative test of spleen lymphocyte transformation was conducted at various time points after immunization.The results indicated that VA5 effectively stimulated the lymphocyte transformation of immune pigeon.Pigeons in each groups were challenged with ND4416 strain at the 30th,90th and 180th d after immunization.The results presented that the NDV+VA5 group had 100% protection rate and higher than La Sota group.The duration of immunization test showed that the antibody titer of NDV+VA5 group reached peak 11.20log2 at the 21st d,remained 7.50log2 at 180th d,and the protection rate remained 100% at 180th d.It indicated that VA5 immunopotentiator sustained the immune duration of pigeon NDV vaccine up to 180 d.Moreover,the in vitro detoxification test results suggested that VA5 immunopotentiator reduced the in vitro detoxification cycle of pigeons after challenge.Overall,this study suggested VA5 immunopotentiator could significantly improve the immune efficacy of pigeon Newcastle disease inactivated vaccine,which provided a basis for further enhancing the efficacy of Newcastle disease vaccine,as well increased experimental data for the application of immunopotentiators.  相似文献   

20.
试验旨在探究VA5免疫增强剂对鸽新城疫病毒（NDV）灭活疫苗（ND4416株）的免疫增效作用。选取160只健康鸽随机分为4组：ND4416株灭活疫苗组（NDV组）、ND4416株灭活疫苗与免疫增强剂（VA5）混合组（NDV+VA5组）、La Sota灭活疫苗组（La Sota组）及生理盐水空白对照组（C组）,进行免疫效力及免疫持续期试验。采用血凝抑制试验检测各组鸽免疫后不同时间点的血清抗体效价,结果显示,VA5免疫增强剂能够显著提高鸽NDV灭活疫苗血清抗体水平（P<0.05）。脾脏淋巴细胞转化试验结果显示,VA5能够有效刺激免疫鸽的淋巴细胞转化。在免疫后第30、90、180天,各组鸽子采用ND4416株进行攻毒试验,结果显示,NDV+VA5组在3个时间点的攻毒保护率均为100%,均高于La Sota组。免疫持续期试验结果显示,NDV+VA5组血清抗体水平在免疫后第21天达峰值11.20log2,第180天仍能达到7.50log2,攻毒保护率达100%,表明VA5免疫增强剂可延长鸽新城疫疫苗的免疫效力持续期至180 d。攻毒后体外排毒检测结果表明,VA5免疫增强剂能够缩短攻毒后鸽的体外排毒周期,减少体外排毒。综上,VA5免疫增强剂能够显著提高鸽NDV灭活疫苗的免疫效果,为研制效果更好的鸽NDV疫苗提供了基础依据,同时也为免疫增强剂的应用增加了试验数据。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号