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1.
斑点免疫结合试验在霉形体研究中的应用 总被引：3，自引：1，他引：2
本文报道了将斑点免疫结合试验(DIBA)用于霉形体种的鉴定,细胞培养中霉形体感染的检测和鸡胚实验感染霉形体的检测,都取得了满意的结果。应用DIBA对11种霉形体培养物和36个自感染细胞和污染疫苗中分离的霉形体的培养物进行了鉴定,结果与直接分离培养物用生长抑制试验,间接免疫荧光检测的结果完全一致。应用口腔霉形体、猪鼻霉形体、精氨酸霉形体和莱氏无胆甾体等4种霉形体的抗血清以DIBA对36株细胞培养物进行检测的结果,证明其中15株感染口腔霉形体,1株感染莱氏无胆甾体,1株为口腔霉形体和莱氏无胆甾体的混合感染,与对照用的分离培养方法结果一致,还有1株用分离培养方法和DNA荧光染色证明有霉形体感染,经鉴定此株霉形体不属于此4种霉形体,故用DIBA未检出。以鸡毒霉形体和滑液霉形体实验感染鸡胚各18枚,以DIBA从这些鸡胚的卵黄液及尿囊液中可检出各自感染的霉形体种。结果说明,在霉形体研究中DIBA为一种快速、简便和特异的血清学方法。 相似文献
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国内火鸡体内霉形体的分离与鉴定 总被引：4，自引：0，他引：4
从北京和呼和浩特市4个火鸡场的95只火鸡、50只火鸡胚和42份精液样品中分离到15个分离物,用国际霉形体分类分会推荐的生化、遗传学和血清学方法证明其中有两个分离物是两种霉形体混合物,共17株霉形体。所有分离物在无抑菌剂的培养基上连续传代5次未出现细菌形态、对毛地黄皂苷敏感、不水解尿素、DNA的G C含量介于26-34mol％之间。生长抑制试验和间接表面免疫荧光技术的试验结果将其中的15个株分属于霉形体属的5个种,即鸡毒霉形体(Mycoplasma gallisepticum)4株、滑液霉形体(M.synoviae)2株、衣阿华霉形体(M.iowae)1株、鸡霉形体(M.gallinarum)和雏鸡霉形体(M.pullorum)各4株。其余2株也属霉形体属,但尚未鉴定到种。 相似文献
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鸡胚制活病毒疫苗中霉形体污染的研究 总被引：3，自引：1，他引：2
对32个鸡胚,80组(每20个鸡胚液混合物为一组)未加抗生素的新城疫疫苗半成品和14批成品疫苗(其中冻干苗12批和液体苗2批)的样品进行了霉形体污染的检查。结果从一个接种了新城疫病毒 LaSota 弱毒株的鸡胚中,9批半成品疫苗中和10批成品疫苗的样品中分离出了霉形体。共20个分离物,对其中12个进行了种属鉴定,12个分离物中有4个含有两种霉形体,因此共含有16株霉形体,即鸡毒霉形体8株,滑液霉形体7株和一株不属于这两种的霉形体。随机选出3个分离物进行了链霉素敏感性测定。测定结果表明这些菌株的抗药性大为增强,链霉素的最小抑制量为对照株的几十百倍,因而能抵抗疫苗中链霉素的作用而污染了疫苗。建议在产品检验中增加霉形体检测项目。在未能使用 SPF 鸡胚生产疫苗以前应尽速进行消灭污染的研究。 相似文献
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鸡源霉形体的分离和鉴定 总被引：2，自引：0，他引：2
对乌鲁木齐市2个大型鸡场鸡毒霉形体（Mycoplasma gallisepticum,MG）、滑液囊霉形体（M.synoviae,MS）感染情况用血清平板凝集试验（SPA）进行了血清抽样调查。结果表明，凝集抗体的阳性率分别为66.92%和35.90%，其中商品鸡场MG和MS的阳性率分别为82.50%和52.50%。从凝似MG、MS感染鸡的气管、肺、气囊、鼻裂和跗关节腔分离到19株分离物。经初步鉴定，分离物均为霉形体属成员。用生化和血清学及其他生物学方法，对这些分离物进行了鉴定。结果6株为MG，3株为MS，其余10株也属于霉形体。 相似文献
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运用DNA重组技术将鸡白细胞介素2基因和鸡毒霉形体H3株TM-1基因进行串联,插入到pET-30a（＋）质粒的EcoRⅠ和HindⅢ多克隆位点间,经PCR鉴定、双酶切鉴定和序列测定,表明已成功构建了含鸡毒霉形体H3株TM-1基因和鸡白细胞介素2基因的融合基因,将含有此融合基因的重组质粒命名为pET-30a（＋）-TM-1-IL-2.将此重组质粒转化E.coli BL21（DE3）菌株.经IPTG 37℃诱导表达4 h后,SDS-PAGE电泳结果显示,此融合基因得到了表达,所表达出的融合蛋白的分子质量约为43 ku,主要以包涵体形式存在.表达产物在尿素存在下经超声波处理,获得了纯化的融合蛋白.这为进一步研究鸡白细胞介素2及鸡毒霉形体的生物学活性奠定了基础. 相似文献
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内蒙古地区肉用仔鸡群中鸡毒霉形体感染情况调查及病原性研究 总被引：1，自引：0，他引：1
应用快速平板凝集试验（SPA）检测内蒙古地区有代表性的10个肉用仔鸡场毒霉形体（MG），平均阳性率为52.7%；对部分阳性鸡进行霉形体分离鉴定，分得的15株霉形体中有12株为MG，将其中的1株接种24日龄肉用仔鸡。证明有很强的致病性。 相似文献
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来源和种类分离数 1262 739 240 197 60 23 20 .3九”︸nU ︼勺精氨酸霉形体莱氏无胆街原体霉形体株70一159无胆街原体sp.牛霉形体乏黄无胆街原体牛眼霉形体 眼无胆角原体啮齿类 关节炎霉形体 肺霉形体禽 鸡败血霉形体 鸡霉形体狗 狗霉形体 霉形体Sp.血清型689总计0 .03八bQ甘O口O乙叮自0。210‘140 .070 .030 .11 9 5 4 5 a 2 31比…1…0分45 268了20百0 .070 14口腔霉形体人型霉形体发酵霉形体唾液霉形体颊霉形体 1929832 63 28 4 ‘卜。日159033.229.5 2 .2 1 .0 0 .1左 0 .072 1 .12 1.022799100 文希震摘译自: CRC Handbook Selics… 相似文献
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我们用微粒凝集反应试验对我国部分省市自治区的兽医研究所和农学院畜牧兽医系从猪呼吸道分离的63个地方菌株进行了培养特性、菌体形态和血清学的检查,证明目前我国猪肺炎霉形体同属于一种,我国猪肺炎霉形体与国际上标准菌株 J 株猪肺炎霉形体也同属于一个种;为进行免疫研究提供了有利条件。微粒凝集反应不仅可用于鉴定菌种(63个地方菌株其中55株为猪肺炎霉形体,5株为猪鼻霉形体,1株为猪滑液霉形体,2株需作进一步鉴定),而且可作为猪场诊断猪地方性肺炎可靠方法,有助于清除带菌猪。它具有特异性高、操作简便等优点。 相似文献
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Mycoplasmas have been implicated in certain clinical syndromes in ostriches and are associated with upper respiratory tract infections. As these infections result in production losses, they are of considerable economic importance to the South African ostrich industry. Although poultry mycoplasmas have been shown to infect ostriches, the existence of unique ostrich-specific mycoplasmas has been suggested. In this study, mycoplasmas were isolated from ostriches in the Klein Karoo, Central Karoo and Garden Route areas of the Western and Northern Cape Provinces of South Africa and identified using 16S rRNA gene sequencing. These sequences indicated that ostriches in these areas carry three unique mycoplasmas and were not infected with chicken mycoplasmas. Phylogenetic analysis of the 16S rRNA sequences of the three isolated ostrich mycoplasmas showed them to be quite divergent and to fall into two distinct phylogenetic groupings. Unique sequences within the 16S rRNA gene of the ostrich mycoplasmas were subsequently used for the development of specific primers for the detection and diagnosis of mycoplasma infections in ostriches. Chickens kept in close proximity to infected ostriches were not infected with these ostrich mycoplasmas. 相似文献
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Mycoplasmas were isolated from two of 43 nasal swabs taken from live horses, and from one of 28 tracheal swabs taken from slaughtered horses. The slaughtered horse that yielded mycoplasmas had no gross pathological changes in the respiratory tract, but the nasal isolations were made from horses with rhinitis. The three mycoplasmas could be distinguished by cultural characteristics, and probably they represent three different species. 相似文献
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In Brazil, mycoplasmas were isolated from the sinuses of Japanese quails (Coturnix coturnix japonica) from two commercial flocks affected with sinusitis. The major respiratory signs and gross lesions are described. Based on serological and biochemical results, the mycoplasmas isolated were identified as Mycoplasma gallisepticum. One of the isolates was pathogenic for chickens. 相似文献
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A study on the prevalence of mycoplasmas in pneumonic bovine lungs was performed on material submitted for diagnostic purposes at the Danish Veterinary Laboratory, Copenhagen. Among the 50 examined cases 43 (86.0%) were found to be infected with mycoplasmas. The predominant mycoplasmas were Ureaplasma spp. (72.0%), M. dispar (48.0%) and M. bovis (24.0%). Other mycoplasmas were M. bovirhinis (20.0%) and M. bovigenitalium (6.0%). Among the infected lungs multiple species infections were predominant (76.7%) over single species infections (23.3%) with M. dispar-Ureaplasma (25.6%), M. bovis-Ureaplasma (18.6%) and M. dispar-M. bovirhinis-Ureaplasma (11.6%) infections being the most frequently encountered combinations. There appears to be an increasing prevalence of M. bovis (24.0%) as compared to earlier reports (0.6-2.0%), thus calling for special attention upon this mycoplasma. Pulsed field gel electrophoresis (PFGE) analysis of 11 field isolates of M. bovis from 9 different farms revealed different profiles except for 2 isolates which were recovered from the same farm. Because mycoplasmas belonging to the 'M. mycoides cluster' were not encountered during this study; it appears that the Danish cattle population is still free from this group of mycoplasma in spite of their presence in some other European countries. 相似文献
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A group of six racing pigeons naturally infected with Mycoplasma columborale and M columbinum were housed in isolation and treated with tiamulin hydrogen fumarate in the drinking water for 35 days. Swabs from the oropharynx, the oesophagus and the trachea were negative for mycoplasmas at the end of this period. Mycoplasmas were recovered from two of the birds after a further nine days, and 13 weeks after the cessation of treatment mycoplasmas were recovered from all six birds. 相似文献
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SUMMARY Mycoplasmas were detected in the external ear canal of goats by swabbing and culture. Up to 108 colony forming units were recovered from single swabs. The resulting cultures were usually mixtures of mycoplasmas containing up to 5 species. The species present in sequential swabs varied. Pathogenic species (M.agalactiae, M.capricolum, M.mycoides subsp. capri, M.mycoides subsp. mycoides of the large colony (LC) type, M.putrefaciens) were isolated from the ears and in addition 3 untyped mycoplasmas G, U and V were often present. The same mycoplasmas were found in large numbers in the mites Psoroptes cuniculi and Raillietla caprae which were sometimes present in the external ear canal. The role of the mycoplasmas in the external ear canal as a source of infection and disease and of the mites in the spread of infection requires further elucidation. 相似文献
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The incidence of Mycoplasma dispar, Ureaplasma and conventional Mycoplasma in the pneumonic calf lung. 总被引：1，自引：1，他引：0 
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下载免费PDF全文 This report describes the incidence of Mycoplasma dispar, ureaplasma and conventional (large colony) mycoplasma isolated from the pneumonic lungs of groups of young calves and the identification to species level of mycoplasmas in mixed populations with the aid of the indirect fluorescent antibody test. Pneumonic lung tissue yielded one or more mycoplasma species from 88% of the 153 calves cultured. The mycoplasmas identified and percent of the calves with lungs positive for each species were: M. dispar (56%), ureaplasma (44%), Mycoplasma bovis (37%), Mycoplasma arginini (33%) and Mycoplasma bovirhinis (23%). Conventional mycoplasmas isolated from two calves (1%) could not be identified using the antisera available. 相似文献
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H H Sch?ttker-Wegner A Binder H Kirchhoff 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1990,37(6):436-441
In the present investigation 262 conjunctival swabs were taken from 178 cattle and examined for mycoplasmas. The isolation was possible from 111 swabs. Mycoplasmas were found in eyes with clinical symptoms of IBK (in 64 of 148 swabs investigated = 43.2%) as well as in healthy eyes (in 47 of 114 swabs investigated = 41.2%). Consequently a correlation between clinical findings and isolation of mycoplasmas could not be observed. Unfortunately 60 of 111 isolates could not be subcultivated after storage at -20 degrees C. Using the indirect immunofluorescence test 41 of the 42 surviving isolates were identified as M. bovoculi which before has not been isolated in the Federal Republic of Germany. One isolate was determined as A. laidlawii. The 17 M. bovoculi strains investigated for their biochemical reactions showed the same characteristics like the reference strain M. bovoculi M 165/69. In repeating examinations mycoplasmas could be isolated 5 times after one month and 14 times after 6 months. Cattle younger than 2 years were more often infected with mycoplasmas (62.5%) than older animals (19.4%). No difference, however, could be observed in the clinical manifestations of IBK between younger and older animals. Mycoplasmas were more frequently isolated in autumn (43.6%) than in spring (21.4%) and summer (29.3%). In most of the animals examined both eyes were colonized by mycoplasmas. No spiroplasmas could be detected in the 262 conjunctival swabs investigated. 相似文献
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L Stipkovits A N Marantidi E K Dzikidze R I Krylova J V Vulvovich 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(2):134-138
Monkeys of the species Presbitus cristata died of severe interstitial pneumonia 20-60 days after their transportation. The carcases were examined for the presence of mycoplasmas. Thirty-six out of 65 samples obtained from the liver, spleen, kidney, lung, lymph nodes, pharynx, bronchi and blood of 13 monkeys contained mycoplasmas biochemically and serologically identical with M. pneumoniae. The isolated strains were able to elicit a rise in body temperature and an elevation in the number of leucocytes in blood as well as development of interstitial pneumonia, colonization of the inner organs by the mycoplasmas and a serological response in the experimentally infected guinea-pigs. 相似文献
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对接种霉形体的原代细胞进行了电镜观察,结果如下:(1)进入细胞内的霉形体,不仅能以包涵体形式存在,也可以散在形式分布,同时还能进入粗面内质网等细胞器内.吸附在细胞膜上的霉形体、包涵体内的霉形体和散在于细胞质中的霉形体均可增殖,而进入细胞核内的霉形体未观察到增殖相.(2)在原代细胞内,正在进行出芽增殖的较大型霉形体内有一个线粒体.(3)进入细胞质中的霉形体,除具有出芽增殖和裂殖增殖形式外,在较大型霉形体内还观察到数个小球形霉形体.霉形体同时可以两种或3种方式增殖.(4)感染霉形体的细胞发生严重的空泡化,细胞体积增大. 相似文献