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1.
本试验旨在研究从断奶到肥育结束,在饲粮中添加微生物植酸酶对猪钙、磷代谢及骨骼发育的影响。试验采用完全随机设计,将72头35日龄断奶的仔猪分为4组,每组3个重复,每个重复6头。试验在相同钙水平基础上设4个组,分别为:1)玉米-豆粕基础日粮(对照组);2)基础日粮 植酸酶-50%磷酸氢钙(处理1);3)基础日粮 植酸酶-75%磷酸氢钙(处理2);4)基础日粮 植酸酶-100%磷酸氢钙(处理3)。试验期按体重分3个阶段:8~20 kg、20~50 kg、50~90 kg,3个阶段植酸酶的添加量分别为750、500和250 U/kg。试验结果表明:(1)在8~20 kg和20~50 kg阶段,添加植酸酶的3个处理,钙、磷消化率显著高于对照组(P<0.05);血钙和血磷浓度,处理1和处理2与对照组无显著差异(P>0.05),处理3显著低于对照组(P<0.05)。在50~90 kg阶段,添加250 U/kg植酸酶代替100%磷酸氢钙,血磷、血钙浓度显著低于对照组(P<0.05);(2)在试验的3个阶段添加植酸酶同时降低饲粮无机磷水平,对血清碱性磷酸酶活性均无显著影响(P>0.05);(3)添加植酸酶使胃中植酸磷消化率显著提高(P<0.0 1),粪中磷排出量显著降低(8~2 0 kg、2 0~5 0 kg阶段,P<0.0 1;5 0~9 0 kg阶段,P<0.0 5);(4)添加植酸酶代替50%或75%磷酸氢钙,猪的掌骨灰分与采食正常磷日粮的对照组无显著差异,但代替100%磷酸氢钙组,猪的掌骨灰分和蹠骨强度则显著低于对照组(P<0.05);添加植酸酶代替50%磷酸氢钙组,猪的蹠骨强度显著高于对照组(P<0.05),代替75%磷酸氢钙组与对照组差异不显著(P>0.05)。总之,在断奶和生长阶段,在猪玉米-豆粕日粮中添加植酸酶可代替部分磷酸氢钙,促进了钙、磷消化利用,促进了骨骼生长,也促进了植酸磷的利用,降低了粪磷排出。  相似文献   

2.
以海蓝褐蛋鸡为试验对象,通过检测蛋鸡血清和肾脏线粒体自由基代谢的变化,研究不同来源高钙日粮对青年蛋鸡血清和肾脏线粒体自由基代谢的影响。选40日龄海蓝褐蛋鸡120羽,随机分成3组,每组40羽,分别饲喂正常钙日粮(以磷酸氢钙形式含钙0.9%,对照组)、石粉高钙日粮(含钙3.78%,试验组1)、磷酸氢钙高钙日粮(含钙3.78%,试验组2),试验期65d。于试验65d,每组随机选取蛋鸡12羽,观察其病理学变化,并分离血清和肾脏线粒体,检测自由基代谢变化。结果显示:试验期间,高钙日粮组均出现青年蛋鸡的异常死亡。试验65d时,与正常对照组相比,石粉高钙组和磷酸氢钙高钙组的左肾重、右肾重、两侧肾总重及肾/体质量比均升高显著或极显著(P〈0.05或P〈0.01),并导致肾脏出现不同程度的病理学损伤;高钙日粮组可导致蛋鸡血清及肾线粒体MDA、NO含量及XOD活性升高(P〈0.05或P〈0.01),T-AOC、GSH-PX及SOD活性下降(P〈0.05或P〈0.01);高钙组组间比较,石粉高钙组表现更严重的组织损伤,血清T-AOC活性降低,与磷酸氢钙高钙组比较差异显著(P〈0.05)。结果提示:高钙日粮可导致青年蛋鸡生长发育的缓慢,肾脏肿大,血清及肾线粒体的自由基生成增多及抗氧化功能的下降。  相似文献   

3.
A case of hypocalcemia was presented with a concurrent hyperphosphatemia. Normal renal function tests excluded primary renal insufficiency. The diet was thought to contain a normal ratio of calcium and phosphorus and, therefore, nutritional secondary hyperparathyroidism was not considered. The radiographs showed normal bone density and the dog was not lame. Concentrations of immunoreactive parathyroid hormone were measured in an attempt to classify the etiology. The dog was successfully managed with calcium supplementation and vitamin D(3) or oral calcium alone.  相似文献   

4.
将120羽40日龄伊沙蛋鸡随机分成3组,每组40羽,分别为:对照组(根据禽类营养需求饲喂正常日粮,含钙0.9%),试验组1(石粉高钙日粮组,含钙3.78%),试验组2(磷酸氢钙高钙日粮组,含钙3.78%),进行为期65d的饲养试验。试验后65d,对各组鸡作血清中钙、磷、尿酸、肌酐和尿素氮的测定,并用免疫组织化学方法检测肾脏中p53蛋白的表达情况。结果表明,高钙组鸡肾脏都有一定程度的损伤,与对照组相比,试验组1中血清尿酸、尿素氮,肌酐显著升高(P〈0.05或P〈0.01),血清磷极显著降低(P〈0.01);试验组2中血清尿素氮极显著升高(P〈0.01),血清磷极显著下降(P〈0.01),血清钙、尿酸差异不显著(P〉0.05);与试验组1相比较,试验组2血清磷极显著升高(P〈0.01),血清钙、尿素氮、尿酸、肌酐差异不显著(P〉0.05)。试验组1鸡肾小管上皮细胞(LLC-PK1)中黄色颗粒很多,p53呈强阳性;试验组2鸡LLC-PK1中黄色颗粒较多,p53呈中等阳性。结果提示,不同钙源高钙日粮能致青年蛋鸡肾损伤,并能够促进肾小管上皮细胞p53蛋白的高表达。  相似文献   

5.
An experiment was conducted to evaluate the effects of dietary boron (B) on growth performance, bone mechanical properties, and calcium (Ca) and phosphorus (P) metabolism in pigs. Thirty-six barrows were weaned at approximately 21 d of age and randomly assigned to receive one of three dietary treatments. Treatments consisted of 1) low-B basal diet (control), 2) basal + 5 mg B/kg diet, and 3) basal + 15 mg B/kg diet. Boron was supplemented as sodium borate. Barrows remained on their respective experimental diets throughout the nursery (35 d) and growing (30 d) phases of production. Blood samples were obtained from each barrow at the end of each phase. Following the 30-d growing period, eight barrows per treatment were transferred to stainless steel metabolism crates. Barrows had an adjustment period of 7 d, followed by a 7-d total collection of urine and feces. All barrows were fed at 90% of the previous ad libitum grower intake of the control animals during the adjustment and collection periods. At the end of the 7-d collection period, barrows were killed and femurs and fibulas were harvested for the assessment of bone mechanical properties. During the nursery phase, ADG and ADFI were increased (P < 0.05) by B supplementation. Boron did not affect (P = 0.34) feed efficiency during the nursery phase. During the growing phase, ADG and ADFI were increased (P < 0.05) by B supplementation. Boron did not affect (P = 0.97) feed efficiency during the growing phase. Boron did not affect (P = 0.44) bone ash percentage, but B supplementation increased (P < 0.05) bone ash P. Ultimate shear force of the fibula was increased (P < 0.05) in barrows supplemented with 15 mg B/kg diet compared to barrows fed diets supplemented with 5 mg B/kg diet. Apparent absorption and retention of Ca and P were not affected (P > 0.05) by dietary B. These data indicate that B supplementation to pigs can increase growth and bone strength without greatly affecting Ca and P metabolism.  相似文献   

6.
Background:Previous studies with broiler have shown dietary supplementation with multi-enzyme complex containing non-starch polysaccharides(NSP) degrading enzymes and phytase is efficient in releasing phosphorus(P),calcium(Ca),energy and amino acids from corn-soybean meal diets or corn-sorghum diets,hence compensating considerable levels of nutrients in formulation.Notwithstanding,such potentials have not been well defined in duck nutrition.Giving China being the largest duck producing country,we conducted this study to establish adequate specifications of major nutrients along with multi-enzyme complex to meat duck from day-old to slaughter,focusing on performance,utilization of nutrients and bone mineralization.Five dietary treatments were:Positive control(PC,T1):the nutrients concentration of diet for 1 to 14 d of age were apparent metabolizable energy(AME) 2,800 kcal/kg,crude protein(CP)19.39%,Ca 0.85%,available phosphorus(avP) 0.42%;for 15 to 35 d of age these parameters were AME2,900 kcal/kg,CP 16.47%,Ca 0.76%,avP 0.38%;Negative control 1(NC1,T2),the AME and digestible amino acids(DAA)were reduced by 70 kcal/kg and 2.0%,avP and Ca by 1.0 g/kg from PC diet;Negative control 2(NC2T4),the downspec from PC diet was AME 100 kcal/kg,DAA 2.5%,avP 1.5 g/kg and Ca 1.2 g/kg;The enzyme complex was added at the same dosage(200 ml7 1,000 kg) on NCI(T3) and NC2(T5) diets.Results:Comparing with the ducks fed on T1,T3 and T5 diets,the birds fed on NC2 diet showed the lowest(P 0.05)body weight(d 14 and 35),feed intake(d 35),tibia ash,Ca and P contents(d 14 and 35),and the utilization of nutrients(P 0.05).The supplementation with the enzyme complex to the NC diets restored growth rate,utilization of nutrients and bone mineralization to the level of the PC diet,and increased AME by 60 kcal/kg and117 kcal/kg,respectively for the NCI and NC2 diets.Conclusion:These results suggest that down-spec AME by 100 kcal/kg,DAA by 2.5%,avP by 1.5 g/kg and Ca by1.2 g/kg caused detrimental effects on duck performance compared with those fed on the PC diet,and these performance losses can be compensated by the addition of the multiple-enzyme complex.  相似文献   

7.
1. In a 42-d feeding trial, 264 one-d-old, as hatched, Cobb 400 broiler chickens (6 pens per group, n = 11 per pen in a 2?×?2 factorial arrangement) were fed on two concentrations of dietary calcium (Ca) (9.0 and 7.5 g/kg in starter, 7.5 and 6 g/kg in grower phases) and supplemental phytase (0 and 500 U/kg diet).

2. During d 0–21, the high Ca + phytase diet improved body weight. During d 0–42, feed intake was increased by the low Ca diet and decreased by phytase supplementation. Feed conversion ratio during d 0–21 was improved by the high Ca + phytase diet.

3. At d 42, Ca in duodenal digesta was reduced by low dietary Ca and supplemental phytase. High dietary Ca reduced P in duodenal and jejunal digesta. Phytase reduced digesta P and increased serum P concentration.

4. Relative tibia length decreased with low dietary Ca and increased with phytase. The robusticity index of tibia was improved by the low Ca diet and phytase supplementation. Phytase supplementation increased tibia ash and concentrations of Ca, magnesium (Mg), manganese (Mn), copper (Cu), zinc (Zn) and iron (Fe) in tibia. The low Ca diet increased Mg, Mn and Fe and reduced Cu and Zn in tibia.

5. It was concluded that 7.5 g Ca/kg during weeks 0–3 and 6 g Ca/kg during weeks 3–6 sustained broiler performance and bone ash, while phytase supplementation facilitated tibia mineralisation, particularly during the grower phase.  相似文献   

8.
1. In a 6-week experiment with broilers, the effect of diet supplementation, using prebiotic fructans, on the performance and biomechanical and geometrical parameters of the tibia and femur bones was evaluated. 2. A total of 240, 1-d-old, Ross 308 chickens were randomly assigned to 1 of 6 treatments, each comprising 5 replicate cages, with 8 birds (4 male and 4 female) per cage. A 2 x 3 factorial arrangement was employed, with two dietary concentrations of calcium and available phosphorus (standard: 9 x 4/9 x 2 g Ca/kg and 4 x 3/4 x 0 g P available/kg; or reduced: 8 x 3/8 x 1 g Ca/kg and 3 x 7/3 x 5 g P available/kg, for the starter/finisher feeding phases, respectively), and with three diets supplemented by selected prebiotics (none; inulin, 7 x 0 g/kg; oligofructose, 7 x 0 g/kg). 3. The concentration of Ca and available P in the diet had no effect on performance parameters at 21 and 42 d of age. At 42 d, reducing the dietary concentrations of Ca and P significantly decreased such biomechanical parameters of bones as tibia stiffness (by 9 x 2%); and femur breaking strength (by 5 x 8%) and stiffness (by 5 x 1%). The diet with a lower concentration of Ca and P negatively affected such geometrical parameter of the tibia as the cross section area, but had no effect on cortex thickness, or bone weight and length. 4. At 21 or 42 d of age, broilers fed on diets supplemented with inulin or oligofructose displayed a performance similar to those fed on the unsupplemented diet. The prebiotics under study also had no statistically significant effects on the biomechanical and geometrical parameters of either bone. There were no significant Ca and P concentration x prebiotics interaction effects, on either performance parameters or on bone quality indices. 5. It was thus concluded that inulin and oligofructose do not improve the performance and bone quality of broilers, either when fed on diets with a standard concentration of Ca and available P, or with reduced concentrations of these macrominerals.  相似文献   

9.
1. A 6-week study was conducted to investigate the effects of phytase and hydroalcoholic extract of Withania somnifera root (WS) on productive performance and bone mineralisation of laying hens in the late phase of production.

2. Diets were arranged factorially (3?×?2?×?2) and consisted of a positive control with adequate Ca (4·37%) and nonphytate P (NPP; 0·39%) and a negative control diet with Ca (4·06%) and NPP (0·36 %); three concentrations of Withania somnifera (0, 65 and 130?mg/kg diet); and two concentrations of microbial phytase (0 and 300 U/kg diet).

3. A total of 144 72-week-old Hy-Line W36 laying hens were randomly assigned to the 12 treatment groups. Each treatment was replicated 4 times (4 x 3 hens). Egg production and egg weight were recorded daily, while feed intake and egg quality traits were recorded every two weeks. Bone quality traits were evaluated at the end of experiment.

4. Withania somnifera supplementation increased egg production and lowered egg weight only in the second two weeks of the experiment. Addition of phytase significantly depressed specific gravity of the eggs for the entire experiment period. No dietary treatment effects were observed on egg shell thickness and yolk weight.

5. Withania somnifera at 130?mg/kg did not affect feed intake. The hens fed on the positive control diet had higher albumen weight than the negative control diet in the second two-week period. Supplementation of the positive control diet with 65?mg/kg Withania somnifera in the absence of phytase significantly improved shell weight compared with the negative control (5·779 vs. 5·273?g respectively).

6. Supplementing Withania somnifera significantly improved Ca and P retention in tibia bone. In addition, an increase in tibia bone P was observed with phytase supplementation. There were significant interactions between Withania somnifera content and phytase for tibia bone Ca and P.

7. The results of this experiment indicated that dietary Withania somnifera has beneficial effects on tibia bone Ca and P content, and phytase improved tibia bone P retention without adverse effects on productive performance.  相似文献   

10.
The objective of this study is to establish a new rabbit model of primary hyperparathyroidism (PHPT) induced by high-phosphate diet. One hundred twenty rabbits were divided into two groups of 60 each. The treatment group was fed a high-phosphate diet (Ca:P = 1:7) and the control group was given a normal animal diet (Ca:P = 1:0.7) for 1 to 6 mo. Serologic examinations, including parathyroid hormone (PTH), calcium and phosphorus levels, blood urea nitrogen, creatinine, and uric acid, and the histologic examination, including parathyroid, kidney, and bones, were performed at the end of each month for 6 mo. Compared with the control, serum PTH levels in the treatment groups were elevated at all six time points, whereas serum calcium levels were reduced, and serum phosphorus levels remain unchanged over the course of the first 3 mo. Serum calcium levels were increased, whereas serum phosphorus levels were reduced at 4, 5, and 6 mo. Parathyroid histopathological examination showed no change during the first month, whereas 60% of the animals exhibited mild hyperplasia starting at 2 mo, and 90% of the animals in the treatment group exhibited mild-to-moderate hyperplasia with gland enlargement starting from 3 mo through the end of the study. Histopathological examination of the kidneys showed no change at 1 mo, but focal parenchymal inflammation with calcium deposition was observed in the treatment groups at 2 to 6 mo. Fibrous tissue of the bone extended toward the cortex, and fibrosis was evident at the third month. The fibrous cells were found to be concentrated mainly on the inner and outer membranes of the bone cortex, and the amount of fibrous tissue increased as the disease progressed. We conclude that a new rabbit animal model of PHPT can be successfully created by the administration of a high-phosphate diet. This animal model can be used in various future studies related to PHPT.  相似文献   

11.
本试验旨在研究低蛋白质饲粮添加α-酮戊二酸(α-ketoglutarate,AKG)对生长猪氮以及钙、磷代谢的影响。试验选用初始体重为(34.98±2.18)kg的健康三元杂交(杜×长×大)猪18头,按体重相近、性别比例相同原则随机分为3组,每组6个重复,每个重复1头。各组AKG的添加水平分别为0(对照组)、1%和2%。先进行为期14 d的饲养试验,观察生长性能;然后进行消化代谢试验,试验期为7 d,前5 d为预试期,后2 d为粪尿收集期(全收粪法)。结果表明:1)1%AKG添加组有改善35~45 kg生长猪生长性能的趋势,具体表现为提高平均日增重(P=0.194 2)和平均日采食量(P=0.258 3),降低料重比(P=0.419 7)。2)随着AKG添加水平提高,尿氮含量有下降趋势(P=0.143 2),粪氮、总氮含量及总氮排放率极显著降低(P0.01),氮表观消化率和蛋白质净利用率极显著提高(P0.01)。与对照组相比,1%与2%AKG添加组尿氮含量分别降低了13.31%和41.88%,粪氮含量分别降低了18.73%和54.69%,总氮排放率分别降低了20.57%和50.00%,氮表观消化率分别提高了2.60%和6.32%,蛋白质净利用率分别提高了2.68%和6.51%。3)2%AKG添加组钙食入量、粪钙和粪磷含量显著低于对照组(P0.05),而钙表观消化率和磷表观消化率却显著高于对照组(P0.05);尿钙和尿磷含量有低于其他各组的趋势,但各组间差异不显著(P0.05)。由此可见,在35~45 kg的生长猪饲粮中添加1%~2%AKG可有效降低机体氮、钙、磷排放,提高氮及钙、磷的利用率和日增重。  相似文献   

12.
本文以鹅为试验动物,采用直接法和差量法研究鹅饲用鸡肉骨粉的营养价值.试验采用单因素完全随机设计,选取260日龄健康五龙鹅和青农灰鹅各42只(公),各设1个对照组和6个试验组,每组6个重复,试验鹅单笼饲养.对照组采用直接法,饲粮中不添加玉米淀粉;6个试验组采用差量法,分别在饲粮中添加15%、25%、45%、65%、75%、85%的玉米淀粉替代鸡肉骨粉.正试期3d,饲喂试验饲粮,然后排空24 h后,连续3d强饲无氮饲粮,每天强饲100 g,采用全收粪法.结果表明:1)五龙鹅和青农灰鹅品种内6个试验组的真代谢能(TME)及粗蛋白质(CP)、粗脂肪(EE)、部分氨基酸(AA)代谢率显著(P<0.05)或极显著(P<0.01)高于对照组;粗纤维(CF)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、钙(Ca)、磷(P)代谢率与对照组相比差异不显著(P>0.05).2)差量法组间比较结果表明,75%玉米淀粉添加组五龙鹅和青农灰鹅的TME及CP、EE、部分AA代谢率均显著高于其他试验组(P<0.05),85%玉米淀粉添加组的各项营养成分代谢率显著低于75%玉米淀粉添加组(P<0.05).CF、NDF、ADF、Ca、P代谢率各组间差异不显著(P>0.05).3)鸡肉骨粉对青农灰鹅TME及CP、部分AA代谢率显著(P<0.05)或极显著(P<0.01)高于五龙鹅;五龙鹅各组CF代谢率显著高于青农灰鹅(P<0.05).试验表明,采用差量法测定鸡肉骨粉的鹅营养成分代谢率数据准确性较高,优于直接法,并且以75%玉米淀粉添加比例效果最好;鸡肉骨粉的鹅营养成分代谢率品种间存在一定的差异性.  相似文献   

13.
Two experiments were conducted to adapt the results of previous studies from our laboratory on calcium and nonphytate phosphorus (NPP) requirements to feeding intervals more similar to the commercial broiler industry. Feeding periods of 0 to 14, 14 to 35, 35 to 42, and 42 to 56 d were used. In the first experiment, a positive control diet similar to industry levels (0.45% NPP and 1.0% Ca, 0.40% NPP and 0.9% Ca, 0.35% NPP and 0.8% Ca, and 0.30% NPP and 0.8% Ca for the various feeding periods, respectively) was compared with various combinations of Ca and NPP levels, with phytase supplementation used in some diets with low NPP. It was observed that birds fed starter diets with 0.40% NPP followed by 0.30% during the grower phase grew as well with equivalent feed conversion as those fed the positive control diet. Feeding less than 0.30% during the grower period resulted in excessive mortality over the duration of the study. In the second experiment, consisting of 2 consecutive trials in which birds were housed beginning on new softwood shavings, adjustments were made in the NPP and Ca levels used in the first experiment; diets were fed with or without phytase supplementation. At the conclusion of the second trial, samples of litter were evaluated for total and soluble P contents. All of the modified diets supported BW gain, feed conversion, livability, and bone parameters that did not differ significantly from that of birds fed the positive control diets. Total and soluble P contents of the litter were significantly reduced by the modified diets fed with or without phytase. Use of the modified diets resulted in significant savings in dietary costs associated with reduced levels of P supplementation.  相似文献   

14.
In a 6 x 6 Latin square arrangement, sheep of 41 kg body weight were fed myristic acid [C14:0; 50 g/kg dry matter (DM)] supplemented to two basal diets of forage : concentrate ratios of 1 : 1.5 and 1 : 0.5 and adjusted to dietary calcium (Ca) contents of either 4.2 or 9.0 g/kg DM (the latter only together with C14:0 supplementation). Various variables of energy, fatty acid and Ca metabolism were determined in combined digestibility and respiratory chamber measurements. With C14:0 addition the energy loss via the faeces increased (p < 0.05, post hoc test) without affecting energy digestibility of the complete diet. The apparent digestibility of supplemented C14:0 was higher (p < 0.01) with approximately 0.8 in the forage-based diet than in the concentrate-based diet (approximately 0.6). The elevated levels of plasma C14:0 were mainly accompanied by reduced C18:0 and C18:1 levels. The estimated apparent content of metabolizable energy (ME) of added C14:0 was either 24.5 MJ/kg (concentrate-based diet) or 32.1 MJ/kg (forage-based diet). Extra Ca equalized these differences between basal diets and ME contents amounted to 33.0 MJ/kg on average. As expected from corresponding slight shifts in energy metabolizability, the total efficiency of ME utilization increased (p < 0.1) with C14:0. The lower level of dietary Ca was still within the range recommended, but adding C14:0 to the concentrate-based diet reduced Ca retention in the body of the sheep from 0.9 to -0.1 g/day because of an impaired (p < 0.05, post hoc test) net Ca absorption, whereas no effect was found with the forage-based diet. With C14:0 addition, plasma total phosphorus (P) and serum calcitrol levels increased (p < 0.05, post hoc test) while Ca concentrations did not clearly reflect the reduced net Ca absorption. Increasing the dietary Ca content prevented adverse effects on Ca retention in the concentrate-based diet and improved Ca retention in the forage-based diet. In conclusion, the C14:0 supplementation reduced Ca availability in concentrate-based diets while an additional supply of Ca improved Ca and energy retention. Consequently, Ca supply should exceed recommended levels in diet types where dietary lipids are likely to reduce Ca availability and a compromise in basal diet type has to found to be able to profit best from the energetic value and the methane-suppressing properties of C14:0.  相似文献   

15.
Interactions between calcium (Ca) and phosphorus (P) were studied in young chickens fed on diets high in n-alkane-grown yeast and in chicks fed on control soya-fishmeal diets for 14 d. Additions of inorganic Ca to diets containing 300 g yeast/kg caused increases in body-weight gain, gain:food ratio and bone mineralisation up to a total dietary concentration of 13.9 g Ca/kg. At all additions of Ca, bone mineralisation was inferior in yeast-fed chicks compared with control chicks. Supplementation of high Ca diets (16.8 g Ca/kg) with inorganic P led to further improvements in body-weight gain, food intake and food utilisation of chicks fed on high-yeast diets. Bone mineralisation also improved but was always inferior in the yeast-fed chicks compared with control chicks. It was concluded that Ca and P supplementation was necessary in high-yeast diets due to low dietary Ca concentrations and low availability.  相似文献   

16.
Sixteen Bonsmara calves (4 males, 12 females) between 10 and 18 months of age were blocked according to age and sex and randomly assigned to 2 groups. They were offered licks containing bone meal and salt (50:50 ratio) (control) and bone meal and ammonium sulphate (NH4SO4) at 1.25, 2.5, 5, 10, 15, and 18% (treatment) to evaluate the effects of dietary anions on bone phosphate (P) concentration. Bone P concentration was significantly (P < 0.05) higher in the NH4SO4 group compared with the control group, indicating that NH4SO4 was able to increase the P content of bone at each of the 6 concentrations used in the lick relative to the control animals, thereby improving the P status of the animals. Ammonium sulphate at 15% and 18 % in the lick also significantly (P < 0.05) increased bone P compared with the lower concentrations of NH4SO4. Bone calcium (Ca) fluctuated as a result of the acidogenic lick. There was absorption of Ca when P was being resorbed and resorption of Ca when P was being absorbed into and out of bone. Bone Ca:P ratio ranged from 3.2 to 6.4 among the control group and 1.6 to 4.3 among the treatment group. Animals receiving the acidogenic lick had a higher percentage ash compared to the control group for most of the experimental period. Bone magnesium (Mg) fluctuated in response to the acidogenic lick, and it was difficult to show a relationship between bone Mg and Ca or P. The overall mean cortical bone thickness was significantly (P < 0.05) greater in treatment (1.60 mm) compared with control (1.43 mm) calves and this was also true at sampling periods 2, 4, 5 and 6. Bone thickness followed bone P and not bone Ca. Results from this research indicate that the addition of ammonium sulphate to a lick had a beneficial effect in improving the P status by increasing bone P and improving the mineral status of bone by increasing the thickness of cortical bone and percentage ash.  相似文献   

17.
Thirty-two crossbred barrows were used to investigate the effects of dietary Ca:total P (tP) ratios in phytase-supplemented diets on the apparent absorption of P and Ca in the small intestine, cecum, and colon. Three Ca:tP ratio treatments (1.5:1, 1.3:1, or 1.0:1) were created by adjusting the amount of ground limestone added to the basal low-P grower (.39% tP including .07% added inorganic P) and finisher (.32% tP without added inorganic P) diets. All low-P ratio diets were supplemented with Natuphos phytase at 500 units/kg. A positive control diet without phytase supplementation contained adequate P and Ca to meet dietary requirements. At 123 kg, the pigs were slaughtered and the contents of ileum, cecum, and colon were collected. Lowering the dietary Ca:tP ratio in the diets containing phytase linearly increased (P < .01) the apparent absorption (% and g/d) of P in the small intestine, but Ca absorption was not affected. Pigs fed the low-P diet with a Ca:tP ratio of 1.0:1 had an apparent absorption (g/d) of P or Ca similar to that of pigs fed the control diet, which was adequate in Ca and P. Averaged across all diets, the apparent absorption of P was highest when measured at the cecum, and the apparent absorption of Ca was highest when measured at the colon. In conclusion, lowering the dietary Ca:tP ratio to 1.0:1 in a low-P diet containing phytase increased the apparent absorption of P in the small intestine. Furthermore, a significant amount of P was absorbed in the cecum.  相似文献   

18.
To evaluate the effect of reduced calcium (Ca) and available P (aP) levels in starter diets on growth performance and tibia, sternum and serum characteristics, and to assay the association between Ca and P (aP) intakes and these variables, 600 one-day-old broiler chicks (Ross 308) were used in a 21-days trial. Broilers were randomly allotted to five treatments with five replicate pens. Chicks were fed on one of the five starter diets that were formulated with a 10% reduction in the Ca and aP contents, starting from the control diet and hence, named as C (0.96% Ca, 0.45% aP), L1 (0.85% Ca and 0.42% aP), L2 (0.77% Ca and 0.38% aP), L3 (0.68% Ca and 0.34% aP) and L4 (0.61% Ca and 0.31% aP). Declining dietary Ca and aP levels did not affect body weight and levels of Ca, P and alkaline phosphatase in serum, but reduced linearly feed intake, the feed conversion ratio and the bone weight and ash content. The Ca and aP intakes and Ca:aP ratio displayed a positive correlation with both tibia and sternum weights, and the mass of the bones were equally sensitive to dietary Ca and aP levels. In conclusion, the body weight of young broilers was irresponsive to changes in the skeleton when exposed to early dietary Ca and aP restrictions (up to 0.61% Ca and 0.31% aP) and the mechanostat had limits due to the association between Ca and aP intakes, and bone properties.  相似文献   

19.
Eight pregnant heifers (primiparous cows) and seven pregnant cows in their second, third and forth pregnancies (multiparous cows) were assigned to two groups and fed either a low calcium (Ca) diet (Ca, 0.46%) or a high Ca diet (Ca, 0.86%) ad libitum from 3 weeks before the expected calving date to 3 days after parturition. All cows were examined for a change in dry matter intake (DMI), plasma minerals and bone turnover around parturition. The dietary Ca level did not affect the DMI in both primiparous and multiparous cows. The DMI of primiparous cows was significantly lower than that of multiparous cows (P < 0.05) in both the low and high dietary Ca groups. The dietary Ca level did not affect the concentrations of plasma Ca, phosphorus, magnesium and parathyroid hormone throughout the experimental period. Plasma phosphorus in primiparous cows was significantly higher (P < 0.05) than that of multiparous cows around parturition. Plasma Ca and magnesium tended to be higher (P < 0.10) in primiparous cows. The dietary Ca level did not affect the plasma osteocalcin (OC) level measured as bone formation or the urinary deoxypyridinoline (DPD) excretion measured as bone resorption before parturition in both primiparous and multiparous cows. After parturition, the plasma OC level was lower than it was before parturition in multiparous cows fed a low Ca diet, and in primiparous cows. There were no significant differences in urinary DPD excretion between each group before and after parturition. Both the plasma OC level and urinary DPD excretion of primiparous cows were significantly higher than those of multiparous cows in both the low and high dietary Ca groups.  相似文献   

20.
1. The objective of this study was to evaluate the effects of total removal of dietary inorganic phosphorus and reduced energy and protein, without and with phytase supplementation, on the performance, egg quality and bone composition of laying hens. 2. Lohmann pink-shell hens were randomly assigned at 56 weeks of age to 5 treatments for 20 weeks as follows: (1) a positive control (PC) with 155 g CP/kg, 11·09 MJ ME/kg, calcium (Ca) 3·40% and non-phytic phosphorus (NPP) 0·26%, (2) a negative control (NC1) diet based on PC diet with Ca decreased to 3·30% and NPP to 0·14%, (3) NC2 diet was formulated on the basis of NC1 diet with 152·7 g CP/kg, 10·90 MJ/kg, (4) NC1 and (5) NC2 supplemented with phytase (300 FTU/kg) each. 3. Feed intake, hen-day or hen-housed egg production, egg number per hen-housed, and final body weight were depressed with NC1 and NC2 diets, but restored by phytase inclusion. There were no significant differences between the dietary treatments for feed conversion efficiency, rates of cracked and broken eggs, egg-shell thickness or egg-shell strength. Mortality was significantly increased by NC2 diet without phytase. Tibia ash was significantly decreased by both NC1 and NC2 diets. Bone strength, and Ca and P contents in tibia ash were significantly increased by phytase inclusion in the NC1 diet. 4. In conclusion, the NC1 and NC2 diets significantly depressed performance and tibia quality, but the addition of phytase (300 FTU/kg) significantly improved performance and tibia integrity.  相似文献   

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