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在绵羊冷冻精液解冻液中添加复合VB提高冻精解冻后品质的研究 总被引：3，自引：2，他引：1
按照L9(34)正交表进行实验设计,将复合维生素B、亚硒酸钠维生素E、谷胱甘肽及抗坏血酸在绵羊冻精解冻液中进行单独或组合添加.6号(50.70%±4.32%)和7号解冻液的解冻后活率(55.35%±2.20%)除与4号解冻液(45.29%±4.11%)差异不明显外,均显著(P<0.05)或极显著(P<0.01)高于其它解冻液.结果表明在解冻液中添加复合维生素B可明显提高冻精的解冻后活率.推荐在绵羊冷冻精液的解冻过程中使用. 相似文献
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选择本地胎次基本相同的母山羊88只,分成两组,利用同期发情技术,分别用人工授精和本交的方法配种.结果表明,波尔山羊冻精配种受胎率为78.5%,本交配种受胎率为89.2%,两组差异不显著(P＞0.05).F1平均初生重3.15kg,本地山羊平均初生重1.81 kg,F1比本地山羊提高74.03%,差异显著(P＜0.05),F1 6月龄平均重35.53 kg,比本地山羊提高93.52%,差异极显著(P＜0.01).可见利用波尔山羊冻精改良当地山羊是一种行之有效的途径. 相似文献
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采用人用胎盘组织液、VB12和3%柠檬酸钠液分别对西门塔尔牛冻精进行解冻。前者解冻后的精子活力分别比后两种药液提高解冻后的精子活力0.070和0.072。采用直肠把握法对本地母黄牛进行输精,各组均输精18头次,试验组受胎率达77.78%,两个对照组的受胎率分别为61.11%和55.56%,试验组的受胎率比两个对照组的平均受胎率提高19.45%,经显著性检验,差异显著(P<0.05),但两个对照组之间的受胎率无显著性差异(P>0.05)。 相似文献
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<正> 对山羊冻精配种技术的研究报道较少,且进展缓慢,大多效果低而不稳。为了摸索提高山羊冻精配种的效果,我们在制作冻精颗粒的稀释液和解冻液配方,输精次数和方法上进行了探讨,以期获得较好的效果。1983年9月至12月,作了山羊冻精配种66只的观察试验,获得了情期受胎率60.97％,产羔率195.23％的效果,现简述于下: 材料与方法 (1)冻精的制作:经汉中地区牧研所初选后提供的稀释液配方:双馏水100毫升、乳糖5.5克。葡萄糖3.0克,柠檬酸三钠1.5克、卵黄一个、甘油5毫升、青链霉素各5—10万单位。对精液采用平衡前后两步稀释,甘油不参与平衡的方法,自作冻精颗粒。原精和解冻后的精子活力不低于0.8和0.3级。 (2)解冻液:采用西乡县畜牧兽医站生产的2.9％柠檬酸三钠2毫升安瓿解冻液。 (3)冻精颗粒分莎能和本地羊两种,输精对象 相似文献
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波尔山羊冻精解冻方法效果的分析 总被引：6，自引：1，他引：5
本文针对影响冻精配种受胎率的各种因素 ,总结分析了几年来在波尔山羊冻精配种中 ,采用不同解冻方法解冻冻精对精子活力、存活时间、顶体完整率、死活染色率以及受胎率几方面的影响 ,结果表明 ,80℃高温干解冻最理想 ,活力 0 3～ 0 4 ,存活时间 1 2～ 1 8h ;80℃高温干解冻优于 4 0℃低温湿解冻(P <0 0 5) ;干解冻效果与温度呈正相关关系 ,以 80℃为最好 ,湿解冻与温度呈负相关关系 ,以 4 0℃为最好 ;精子存活时间与受胎率呈正相关关系 ;一次性解冻一粒冻精活力最好 ,两项指标均超过一次解冻多粒冻精 (P <0 0 5) ,一次干、湿解冻 2、3、4粒冻精 ,各项指标间差异不显著 (P >0 0 5) ,且活力、存活时间、顶体完整率、活精子染色率等指标均达到冻精配种要求 (活力 0 3) ,实际配种情期受胎率达 50 %以上。因此 ,在实际生产中 ,可采用高温 ( 80℃ )干解冻和低温 ( 40℃ )湿解冻两种方法 ,一次性解冻 1～ 4粒冻精 ,既能保证受胎率 ,又可节省精液和时间 ,提高工作效率 相似文献
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波尔山羊改良沂蒙山区当地山羊的效果 总被引：1，自引：0，他引：1
临沂市自1999年以来用引进波尔山羊及其冻精对沂蒙山区当地山羊进行杂交改良,几年来的结果表明,其冻精配种情期受胎率为43.54%,鲜精20倍稀释配种平均受胎率为68.74%,产羔率达198%。初生个体重达3.37±0.74kg;其生理常值均在正常范围。周岁龄屠宰活重、屠宰率、净肉率分别比当地山羊提高76.43%、30.38%和32.21%,差异极显著(P<0.01)。这说明用波尔山羊改良沂蒙山区当地山羊,是一条发展肉羊生产的重要途径。 相似文献
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笔者从事山羊的冷冻精液人工授精技术推广数年,觉得在冻精质量较为可靠的前提下,影响受胎率的关键是解冻方法,输精时间及输精部位等技术环节。把握得当,一个情期输精2次,平均情期受胎率可达65%以上,做法和体会如下。1解冻山羊冻精的解冻以40~60℃快速解冻为宜。1.1颗粒解冻有两种方法1.1.1干解冻法试管洗净、灭菌后用解冻液冲洗,倒尽余液,置于45~60℃水浴锅或保温杯中预热至同温后,投入1粒冻精颗粒,迅速摇动试管,待颗粒溶化至2/3时从热水中取出试管,用双手迅速搓动使颗粒全部溶解均匀即成。1.1.2湿解冻法将盛有1 mL解冻液(一般用2.9%柠檬酸… 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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