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1.
为掌握豫南地区规模化猪场中猪繁殖与呼吸综合征病毒（PRRSV）和猪圆环病毒2型（PCV2）疫苗免疫场猪群抗体水平和非疫苗免疫场猪群病原感染情况,试验利用ELISA方法对该地区猪群进行了PRRSV和PCV2抗体水平检测。结果表明,在疫苗免疫猪群中,PRRSV和PCV2抗体合格率分别为68.8%和58.7%,规模越大的猪场合格率越高,200~500头母猪规模场抗体阳性率最高（分别为76.8%和77.4%）,50头母猪以下场最低（分别为48.3%和44.2%）,种猪的合格率均最高,而育肥猪群合格率均最低。在非疫苗免疫猪群中,PRRSV和PCV2抗体阳性率分别为55.6%和65.3%,PRRSV抗体阳性率最低的是100~200头母猪规模场（46.5%）,最高的是50头母猪以下规模场（68.8%）,断奶仔猪和育肥猪群抗体阳性率均在71%以上;随猪场规模越大,PCV2抗体阳性率越低,200~500头母猪规模场抗体阳性率为41.4%,50~100头规模场和50头以下场的阳性率分别为78.9%和78.6%,种公、母猪的PCV2抗体阳性率最低（分别为37.5%和40.4%）,断奶仔猪和育肥猪抗体阳性率较高（分别为82.2%和79.5%）。本研究反映了豫南地区猪繁殖与呼吸综合征（porcine reproductive and respiratory syndrome,PRRS）和猪圆环病毒病（porcine circovirus disease,PCVD）疫苗免疫猪场的疫苗免疫效果和非疫苗免疫猪场病原感染的实际情况和规律,为该地区PRRS和PCVD防制工作提供了理论依据和指导。 相似文献
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为了解贵州省某猪场几种病毒性疫病免疫抗体水平及感染情况,首先采用ELISA抗体检测方法,对该猪场送检的415份血清样本进行了猪口蹄疫(FMD)、猪瘟(CSF),猪繁殖与呼吸综合征(PFRS)、猪乙型脑炎(JE)、猪伪狂犬病(PR)、猪圆环病毒病(PCVD)及猪细小病毒感染(PPI)的抗体水平检测,结果:FMD、CSF、PRRS、JE、PR、PCVD及PPI抗体阳性率分别为95.4%,61.0%、74.0%、78.6%、89.4%、72.5%、42.9%;进一步对送检样品应用PCR/RTPCR进行病原检测,电泳结果FMDV、CSFV、PFRSV、JEV与PRV均未出现目的条带,PCV2与PPV出现预期大小条带,表明该猪场存在PCV2与PPV野毒感染,提示该猪场应对猪瘟抗体水平不合格的猪群进行补免,同时加强对猪圆环病毒病与猪细小病毒病的免疫预防。 相似文献
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为了解贵州省某规模化猪场4种猪常见病毒性疫病的抗体水平及感染情况,实验采用ELISA抗体检测法,对该猪场送检的370份血清进行了CSFV、PRRSV、PCV2和PRV 4种抗体水平检测。实验结果表明:该猪场种猪CSFV、PRRSV、PCV2和PRV抗体阳性率分别为100%、94.74%、100%、5.26%,保育猪CSFV、PRRSV、PCV2和PRV抗体阳性率分别为94.44%、83.33%、16.7%、5.56%;其中种猪CSF、PRRS、PCV2、PR已接种疫苗;保育猪CSF、PRRS、PR已接种疫苗,未接种PCV2疫苗,上述数据说明该猪场种猪CSF、PCV2、PRRS、PR免疫效果较好;保育猪CSF、PRRS、PR免疫效果较好,PCV2有野毒感染现象。该猪场种猪应在保育猪阶段做好PCV2的预防接种工作,同时做好猪场的疫病净化措施。 相似文献
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为有效评估益阳地区中小规模猪场猪群的免疫水平,选择78个中小规模猪场(存栏母猪30~150头)抽取312份血清样品,使用ELISA方法检测口蹄疫(FMD)、猪瘟(CSF)、猪繁殖与呼吸综合征(PRRS)、猪圆环病毒相关疾病(PCVAD)、猪伪狂犬病(PR)等5种猪群主要病原的抗体。结果显示,猪群FMDV的抗体平均合格率为60.26%,PR R SV的抗体平均合格率为77.56%,CSFV的抗体平均合格率为83.33%,PRV的抗体平均合格率为69.87%,PCVADV的抗体平均合格率为95.51%;对该地区代表猪场的免疫抗体水平有了基本认识。 相似文献
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为摸清当前安徽省猪群中PCV2感染情况及疫苗的免疫效果,本试验采集了12个猪场的289份猪扁桃体和289份猪血清样品,采用荧光定量PCR方法检测扁桃体中的PCV2,采用ELISA方法检测血清中PCV2抗体。试验结果显示:猪群PCV2感染率为11.8%,其中大型猪场、中型猪场、小型猪场感染率分别为8%、0%、26%,生产母猪、后备母猪、保育猪及育肥猪感染率分别为12%、16%、0%、17%;猪群PCV2抗体阳性率92.4%,其中大型猪场、中型猪场、小型猪场阳性率分别为96%、82%、98%,生产母猪、后备母猪、保育猪、育肥猪阳性率分别为100%、91%、82%、98%。安徽省猪群存在PCV2隐性感染,其中小型猪场及育肥猪群感染率较高;不同养殖规模、不同生长阶段猪群猪圆环病毒疫苗免疫效果良好。 相似文献
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《黑龙江畜牧兽医》2016,(4)
为了分析黑龙江某猪场仔猪发病情况与疫苗免疫情况的关系,试验采用PCR方法对患病仔猪病原进行了鉴定,同时随机抽检了该猪场425份猪血清,采用ELISA法对猪瘟(CSF)、伪狂犬病(PR)、猪圆环病毒(PCV-2)和猪繁殖与呼吸综合征(PRRS)疫苗免疫的抗体进行了检测。结果表明:发病仔猪系伪狂犬病毒(PRV)、圆环病毒(PCVD)和猪繁殖与呼吸综合征病毒(PRRSV)三种病原混合感染;种猪、后备母猪和妊娠母猪的四种疫苗抗体合格率均高于国家规定标准;21日龄仔猪的抗体合格率整体偏低;56日龄仔猪和100日龄仔猪的CSFV、PRRSV抗体合格率较高;100日龄仔猪的PRV抗体合格率偏低。 相似文献
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《养猪》2020,(4)
为分析评估贵阳市某规模化种猪场猪群健康状况,进一步推动全市规模化种猪场主要动物疫病净化,采用酶联免疫吸附试验(ELISA)和实时荧光PCR/RT-PCR试验分别对362份猪血清和猪扁桃体组织进行了猪口蹄疫(FMD)、猪瘟(CSF)、猪繁殖与呼吸综合征(PRRS)和猪伪狂犬病(PR)的抗体水平及病原检测。结果:FMD、CSF、PRRS和PR的免疫抗体阳性率依次为90.61%、94.75%、88.12%和96.96%;FMD和PR的野毒感染抗体阳性率分别为1.38%和3.59%;FMD、CSF、PRRS和PR的病毒核酸阳性率依次为0、0.83%、0和1.38%。试验结果表明,该规模化种猪场4种病毒性疫病的疫苗免疫效果较好,但存在CSF和PR野毒感染情况,建议今后应继续加强疫苗免疫,并立即对检出的CSF和PR病毒核酸阳性猪只进行淘汰处理,同时加强对FMD和PR感染抗体阳性猪只的隔离和监控。 相似文献
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为了解贵州省六盘水市某规模化猪场6种猪常见病毒性疫病的抗体水平及感染情况,试验采用ELISA抗体检测方法,对该猪场送检的589份血清进行了CSF、FMD、PRRS、PCVD、PPI及JE抗体水平检测。试验结果表明:该猪场CSF、FMD、PRRS、PCVD、PPI和JE抗体阳性率分别为83.19%、95.08%、84.21%、48.05%、44.14%、39.56%;其中CSF、FMD和PRRS已接种疫苗,PCVD、PPI和JE未接种疫苗,上述数据说明该猪场CSF、FMD和PRRS免疫效果较好,PCV2、PPV和JEV存在野毒感染现象,其中PCVD较为严重,提示该猪场应加强PCVD、PPI和JE的预防。 相似文献
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在2015-2016年期间,每季度对辖区内母猪存栏量50头以下、母猪存栏50头以上各3个猪场进行采样监测,每场采集20份血清样品,每季度采集血清样品120份,采用ELISA方法对2015-2016年梧州市规模猪场猪瘟(CSF)、猪繁殖与呼吸综合征(PRRS)、猪伪狂犬病(PR)及口蹄疫(FMD)免疫抗体进行动态监测,结果表明:从季节上比较分析,口蹄疫、猪瘟与猪伪狂犬病免疫抗体消长随季度呈现波浪式变化,其中第二、四季度分别比第一、三季度的抗体合格率高,猪蓝耳病免疫抗体第二、三、四季度抗体合格率比第一季度抗体合格率高;从年份比较分析,2016年口蹄疫、猪瘟、猪伪狂犬病与猪蓝耳病免疫抗体整体水平优于2015年抗体水平。 相似文献
10.
【方法】采用间接ELISA方法,对采自23个规模场户的419份血清,进行PRRSV变异株疫苗免疫抗体检测。【结果】17个场户免疫抗体合格率在70%以上,平均合格率为79.71%,其中种猪为90.52%、保育猪为80.17%,育肥猪为70.48%;11个灭活疫苗免疫场户中有6个场户免疫抗体合格率在70%以上,平均抗体合格率为71.48%,12个弱毒疫苗免疫场户中有10个场户免疫抗体合格率在70%以上,平均抗体合格率为87.55%。【结论】灭活疫苗免疫种猪、保育猪和育肥猪抗体合格率存在明显差异,弱毒疫苗免疫种猪和保育猪与育肥猪抗体合格率之间存在明显差异。 相似文献
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Breed differences for weight (CW), height (CH), and condition score (CS) were estimated from records (n = 12,188) of 2- to 6-yr-old cows (n = 744) from Cycle IV of the U.S. Meat Animal Research Center's Germplasm Evaluation (GPE) Program. Cows were produced from mating Angus and Hereford dams to Angus, Hereford, Charolais, Shorthorn, Galloway, Longhorn, Nellore, Piedmontese, and Salers sires. Samples of Angus and Hereford sires were 1) reference sires born from 1962 through 1970 and 2) 1980s sires born in 1980 through 1987. The mixed model included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and breedgroup as fixed effects for CW and CS. Analyses of weight adjusted for condition score included CS as a linear covariate. The model for CH excluded PL. Random effects were additive genetic and permanent environmental effects associated with the cow. Differences among breed groups were significant (P < 0.05) for all traits and were maintained through maturity with few interchanges in ranking. The order of F1 cows for weight was as follows: Charolais (506 to 635 kg for different ages), Shorthorn and Salers, reciprocal Hereford-Angus (HA) with 1980s sires, Nellore, HA with reference sires, Galloway, Piedmontese, and Longhorn (412 to 525 kg for different ages). Order for height was as follows: Nellore (136 to 140 cm), Charolais, Shorthorn, Salers, HA with 1980s sires, Piedmontese, Longhorn, Galloway and HA with reference sires (126 to 128 cm). Hereford and Angus cows with reference sires were generally lighter than those with 1980s sires. In general, breed differences for height followed those for weight except that F1 Nellore cows were tallest, which may in part be due to Bos taurus-Bos indicus heterosis for size. 相似文献
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In experiment 1, 6 pregnant mares received a concentrate that contained a trace mineral premix that provided 14.3 mg Cu, 40 mg Zn, 28 mg Fe, 28 mg Mn, 0.08 mg Co, 0.16 mg I, and 0.16 mg Se/kg concentrate (group A). Seven mares received the same concentrate plus 502 mg Zn and 127 mg Cu once daily (group B). No differences (P > .05) in foal growth data, or Cu, Zn, and Fe concentrations of mare milk, mare serum, or foal serum were observed. In experiment 2, 6 pregnant mares received the same concentrate as group A (group C), and 8 mares received the same concentrate fortified with 4× the trace mineral premix (group D). Group C mares had higher serum Zn concentration at 1 day (P < 0.01) and 56 days (P < 0.04). Group C mares had higher milk Fe concentration at 28 days (P < .01), and group D mares had higher milk Cu concentration at 56 days (P < .01). Group C foals had higher serum Cu concentration at 14 days (P < .03). The results from this study provide no evidence to indicate that supplementing late gestating and lactating mares with higher dietary trace mineral levels than those recommended currently by NRC has any influence on foal growth and development, or on the Cu, Zn, and Fe concentrations of the mare milk, mare serum, or foal serum. 相似文献
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Nadine Pa?lack Barbara Mainzer Monika Lahrssen-Wiederholt Helmut Schafft Richard Palavinskas Angele Breithaupt Jürgen Zentek 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):57-66
This study was conducted to measure the concentrations of strontium (Sr), barium (Ba), cadmium (Cd), copper (Cu), zinc (Zn), manganese (Mn), chromium (Cr), antimony (Sb), selenium (Se), and lead (Pb) in canine liver, renal cortex, and renal medulla, and the association of these concentrations with age, gender, and occurrence of chronic kidney disease (CKD). Tissues from 50 dogs were analyzed using inductively coupled plasma mass spectrometry. Cu, Zn, and Mn levels were highest in the liver followed by the renal cortex and renal medulla. The highest Sr, Cd, and Se concentrations were measured in the renal cortex while lower levels were found in the renal medulla and liver. Female dogs had higher tissue concentrations of Sr (liver and renal medulla), Cd (liver), Zn (liver and renal cortex), Cr (liver, renal cortex, and renal medulla), and Pb (liver) than male animals. Except for Mn and Sb, age-dependent variations were observed for all element concentrations in the canine tissues. Hepatic Cd and Cr concentrations were higher in dogs with CKD. In conclusion, the present results provide new knowledge about the storage of specific elements in canine liver and kidneys, and can be considered important reference data for diagnostic methods and further investigations. 相似文献
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《饲料工业》2019,(18):54-58
应用电感耦合等离子-质谱技术(ICP-MS),建立饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅等元素的测定方法。对饲料样品的前处理方法、仪器工作参数和11种元素标准曲线进行优化;并以加标回收、分析方法比对和重复测试说明方法的准确性和精密性。方法在0～1 000 ng/ml范围内线性良好,仪器检出限为0.557 7～5.072 ng/ml,具有良好的精密度,其回收率在88.1%～104.4%之间,相对标准偏差小于5.0%。同时与原子吸收和原子荧光方法进行比对,测定结果相近。所建立的方法简单、快速,可替代原子吸收和原子荧光方法测定饲料中的11种金属元素,为饲料的质量控制提供理想的元素分析方法。 相似文献
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K Reifenberg M Stahl U L?sch 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(3):191-198
A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichment of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity, and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays. 相似文献
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《The Journal of small animal practice》1975,16(1-12):143-154
Circular excised skin wounds in the thoracic and metatarsal regions of the dog were studied. A similar sequence of events took place in the two regions although differences did occur due to the different reactions of the tissues which surrounded the wounds. None of the wound cavities became filled with exudate during the early stages of healing. In the thoracic wounds the cavities were largely filled by the swelling and inward movement of adipose tissue. Epithelium first grew on the wound surface in the sector of the wound that was situated in the direction of hair flow. The average time to complete epithelization was similar in both sets of wounds. A zone of alopecia developed around the wounds. 相似文献