共查询到18条相似文献，搜索用时 234 毫秒
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1998年8月美国北卡罗来那州的一位兽医报告,在一个对Ｈ1Ｎ1猪流感病毒作了常规免疫接种的猪群中发生了看来是非常严重的猪流感病例。母猪发病极为严重,多有死亡,并且因流产而造成极大的繁殖损失。实验室警觉起来:猪群中可能存在着一种新的猪流感亚型。对此,除了进行通常的快速抗原俘获ＥＬＩＳＡ试验进行证实以外,还分离到了病毒。分离到的病毒是双重重组猪流感病毒,它获得了人Ｈ3Ｎ2流感病毒的血凝素和神经氨酸苷酶,这种病毒在20年前就已经出现在了欧洲和亚洲。特别的是,在美国新分离到的这种病毒具有来自人流感病毒的血凝素、神经氨酸苷酶… 相似文献
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2009年发生于北美,流行于世界的流感疫情为A型流感病毒,H1N1亚型猪流感病毒毒株。该毒株包含有猪流感、禽流感和人流感三种流感病毒的基因片断,是一种可以人传染人的新型猪流感病毒,这种新型病毒由猪流感病毒演变而成。 相似文献
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猪流感病毒分型基因芯片的建立和初步应用 总被引：9，自引：0，他引：9
根据流感病毒血凝素（HA）和神经氨酸酶（NA）亚型基因序列间的差异性,分别设计H1、H3、H9、N1和N2的特异分型引物,并根据M基因设计A型流感病毒的通用引物。RT-PCR扩增猪流感病毒各亚型HA、NA和M基因的特异片段,克隆至pMD18-T构建重组质粒,制备探针。将各探针按一定的阵列点加到硝酸纤维素膜上,制成基因芯片。并对217份不同地区的样品进行检测。结果表明：该芯片可以同时检测待检样品中5种亚型A型流感病毒,并显示了较高的灵敏度和特异性,灵敏度比PCR高1个稀释度,比病毒分离高2个稀释度。该方法的建立为猪流感的检测及猪流感病毒各亚型在猪群中的流行病学调查提供了一种快速、灵敏和高通量的手段。 相似文献
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曹向英 《上海畜牧兽医通讯》2007,(4):43-44
猪流感（Swine influenza，SI）主要是由正粘病毒A型流感病毒引起的一种急性、高度接触性呼吸道传染病。临床上以呼吸困难、咳嗽、高热、妊娠母猪流产为主要特征。流感病毒极易发生变异，尤其是其表面血凝素和神经氨酸酶的抗原性转移可导致新亚型流感病毒的出现，自1918年至今，已发生过5次大变异，每次变异都造成大流行。为了解和掌握上海市松江地区规模场、养猪专业户及农民散养户猪流感的疫情状况， 相似文献
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猪流感病毒蛋白研究进展 总被引：4，自引：4，他引：0
猪流感（swine influenza,SI）是由猪流感病毒（swine influenza virus,SIV）引起的猪的一种传染病,其在世界各地的广泛存在和流行,给养猪业带来了巨大的经济损失。猪流感病毒属于正黏病毒科A型流感病毒属,作者就猪流感病毒蛋白,包括血凝素（HA）、神经氨酸酶（NA）、核蛋白（NP）、基质蛋白（M）、聚合酶蛋白（PA、PB1和PB2）和非结构蛋白（NS）进行简要概述,以期为猪流感病毒的致病机制、诊断、分子流行病学等方面的研究提供参考。 相似文献
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美国发现有禽流感基因的猪流感病毒HN 《畜牧与饲料科学》2008,29(1)
美国的研究人员已经发现了一种新的猪流感病毒H2N3,该病毒属于H2流感病毒组,是由禽流感和猪流感的基因分子共同组成的。科学家从猪流感 相似文献
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猪流感病毒研究进展 总被引：11，自引：1，他引：10
猪流感是由猪流感病毒引起的一种呼吸道传染病。目前 ,此病在世界各地都有发生 ,危害严重 ,经济损失巨大 ,并对人类的健康构成威胁。猪流感病毒属于正黏病毒科 A型流感病毒属 ,病毒粒子多形态。该病毒对热、消毒剂敏感 ,而对干燥和低温的抵抗力强大。其分子特性为多节段的 RNA病毒 ,由 8个片段组成 ,分别编码 1 0种蛋白质。猪流感病毒能够在多种动物的细胞上增殖 ,但病毒分离和疫苗生产时 ,经常采用鸡胚接种。病毒具有血凝活性 ,但不同毒株的抗原性无明显的区分。由于病毒受到抗体的压力很大 ,因此病毒的变异频繁 ,其机理涉及分子水平的抗原漂移和抗原转变。文章对病毒的理化、生物学特性、分子特性、病毒的蛋白和基因变异等方面的研究情况进行了综述 相似文献
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Zhou NN Senne DA Landgraf JS Swenson SL Erickson G Rossow K Liu L Yoon KJ Krauss S Webster RG 《Veterinary microbiology》2000,74(1-2):47-58
In late summer through early winter of 1998, there were several outbreaks of respiratory disease in the swine herds of North Carolina, Texas, Minnesota and Iowa. Four viral isolates from outbreaks in different states were analyzed, both antigenically and genetically. All of the isolates were identified as H3N2 influenza viruses with antigenic profiles similar to those of recent human H3 strains. Genotyping and phylogenetic analysis demonstrated that the four swine viruses had emerged through two different pathways. The North Carolina isolate is the product of genetic reassortment between human and swine influenza viruses, while the others arose from reassortment of human, swine and avian viral genes. The hemagglutinin genes of the four isolates were all derived from the human H3N2 virus circulating in 1995. It remains to be determined if either of these recently emerged viruses will become established in the pigs in North America and whether they will become an economic burden. 相似文献
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Bosworth B Erdman MM Stine DL Harris I Irwin C Jens M Loynachan A Kamrud K Harris DL 《Comparative immunology, microbiology and infectious diseases》2010,33(6):e99-e103
An alphavirus derived replicon particle (RP) vaccine expressing the cluster IV H3N2 swine influenza virus (SIV) hemagglutinin (HA) gene induced protective immunity against homologous influenza virus challenge. However, pigs with maternal antibody had no protective immunity against challenge after vaccination with RP vaccines expressing HA gene alone or in combination with nucleoprotein gene. 相似文献
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Antigenic characterization of an H3N2 swine influenza virus isolated from pigs with proliferative and necrotizing pneumonia in Quebec. 总被引：2，自引：0，他引：2 
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下载免费PDF全文 M H Bikour E Cornaglia J M Weber Y Elazhary 《Canadian journal of veterinary research》1994,58(4):287-290
A new strain of swine influenza A virus, designated A/Swine/Saint-Hyacinthe/150/90 has been isolated from pigs with severe proliferative and necrotizing pneumonia in Quebec. The antigenic characterization of the hemagglutinin was performed by hemagglutination inhibition test, immunoblot and indirect immunoprecipitation using polyclonal antisera. Only the last test was able to detect an antigenic relationship between the hemagglutinin of this isolate and an H3 subtype influenza virus. The immunoprecipitation test was a useful alternative for determining the hemagglutinin of influenza A virus subtypes. The neuraminidase inhibition test demonstrated a reactivity between the A/Swine/Saint-Hyacinthe/150/90 and antiserum against a N2 subtype influenza virus. Our results indicate that this new strain isolated for the first time in the porcine population of Canada is related to A/Sw/Hong Kong/76 H3N2 swine influenza virus. 相似文献
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Zhao G Fan Q Zhong L Li Y Liu W Liu X Gao S Peng D Liu X 《Research in veterinary science》2012,93(1):125-132
To investigate whether the 2009 pandemic H1N1 influenza A virus was still being transmitted in swine, a total of 1029 nasal swab samples from healthy swine were collected from January to May 2010 in Jiangsu province of China. Eight H1N1 influenza viruses were isolated and identified, and their full length genomes were sequenced. We found that all eight of the H1N1 viruses shared higher than 98.0% sequence identity with the 2009 pandemic virus A/Jiangsu/1/2009 (JS1). In addition, some of these viruses had D225G (3/8) mutations in the receptor binding sites of the hemagglutinin (HA) protein, indicating enhancement of their binding affinity to the sialic α2, 3Gal receptor. In conclusion, the 2009 pandemic H1N1 influenza A virus has retro-infected swine from humans in mainland China, and significant viral evolution is still ongoing in this species. 相似文献
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