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将114只15日龄“伊莎”公鸡,随机分为两组(实验和对照组),每组57只。实验组以15勒克斯的照度,9小时明;3小时暗:5小时明:7小时暗的间歇光照周期补加光照,使每天的光照时间达14小时,对照组仅接受自然光照。结果表明:不同日龄实验组鸡的体重均大于相应的对照组,而且在55日龄和75日龄时两组鸡的体重间相差极显著;各日龄段,实验组鸡群的料肉比均略低于相应的对照组;实验组鸡的射精量、精子密度略高于对照组,一次射精的精子总数显著的多于对照组,而死精子百分数低于对照组。 相似文献
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将72只日龄为14d的“星布罗”肉用仔鸡随机分为实验和对照两组,每组36只。实验组接受强度为151x的1L:3D间歇性白炽灯光照,对照组为自然光照。两组的饲喂条件相同。结果:与对照组相比,实验组体重略高而料肉比略低,实验组鸡胰腺A、B细胞的内分泌颗粒减少,A颗粒粒芯周围出现大致均匀的晕轮,B颗粒粒芯呈现不典型的针状、棒状或环状,PP细胞的内分泌颗粒明显增大、增多,胞浆中的粗面内质网、高尔基复合体和线粒体等数量增多。两组鸡的D细胞无明显差异。34日龄的实验组鸡血浆胰岛素含量明显高于对照组,且与体重呈正相关。 相似文献
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通过观测不同光照下肉仔鸡自由基和抗氧化酶指标的变化以探讨光照对其的影响。240羽AA肉仔鸡分为对照组(C)、光照1组(L1)和光照2组(L2),于7～30日龄期间分别采取24L:0D、22L:2D和20L:4D的光照措施,30日龄后均恢复为连续光照。从14～42日龄每周随机抽取各组鸡10羽采集血清,检测SOD、GSH-PX和MDA的含量。结果表明,从14～42日龄,间歇光照提高了肉仔鸡SOD、GSH-PX的活性而降低了MDA的含量,但仅L2组的SOD于35日龄显著高于C组(P<0.05),其余各值与对照组间均无显著差异(P>0.05)。试验结果提示L1和L2两种间歇光照能够起到提高肉仔鸡的抗氧化酶活性和降低自由基的作用,但效果不显著。 相似文献
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不同光照制度对黄羽肉鸡蛋白质沉积及血浆中甲状腺激素浓度的影响 总被引：3，自引：0，他引：3
试验选用5周龄石歧杂公鸡450只 ,随机分成5组 ,每组90只 ,每组3个重复 ,每重复30只鸡 ;Ⅰ组为对照组 ,Ⅱ、Ⅲ、Ⅳ、Ⅴ为试验组 ,Ⅰ组给予自然光照 ,Ⅱ、Ⅲ、Ⅳ、Ⅴ组分别给予12L(明) :12D(暗)、6L:6D、3L:3D、2L:2D人工间歇光照。试验期鸡的基础日粮含代谢能12.93MJ/kg,CP16.78 %。鸡只自由采食和饮水 ,记录统计每日采食量。从40日龄开始 ,每隔2周称重 ,且从每重复中随机抽取6只鸡 ,其中3只用以采血制血浆 ,用RIA法测血浆中甲状腺素(T4)、三碘甲腺原氨酸(T3)浓度 ;另3只鸡 ,采用闷杀法 ,用以测定机体蛋白质含量。结果表明 :(1)在沉积蛋白质总量、蛋白沉积率方面 ,第Ⅴ组在各时期均最高 ,且显著(P<0.05)或极显著(P<0.01)地高于对照组。(2)对照组T3 浓度随日龄的增大而呈下降趋势 ,T4 浓度与此相反 ,有上升趋势 ;试验各组T3 浓度低于对照组 ,而T4 浓度高于对照组 ;其中除第Ⅳ组外 ,其它试验组的T3 浓度在54、68、82日龄均显著低于对照组(P<0.05) ,第Ⅲ、Ⅴ组在各时期T4 浓度显著高于对照组(P<0.05)。结果提示 :人工间歇光照能促进蛋白质的沉积(其中以2L :2D沉积效率最高) ,还能降低血浆T3 浓度 ,提高T4 浓度 相似文献
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单色光对肉鸡睾丸形态结构的影响 总被引：2，自引：0，他引：2
采用LED（Light-emitting diodes）灯作为光源,比较单色光对雄性AA肉鸡睾丸形态结构的影响,并对其内在机理进行探讨。将48只刚出壳的AA肉鸡分为4个处理组,试验组为红光组（660 nm）、绿光组（560 nm）和蓝光组（480 nm）,对照组为白光组（400-760 nm）,每组12只,自由采食。光照强度为15 lx,光照制度23 h∶1 h（L：D）。49日龄利用放射免疫法检测血清中甲状腺激素和睾酮的含量,并测量体重、睾丸重、睾丸体积、曲精细管面积和生精细胞百分数。结果表明,蓝、绿光对睾丸的影响较显著,蓝、绿光组的体重、睾丸重、睾丸体积、曲精细管面积和生精细胞百分数显著高于红、白光组7.22%-32.37%（P〈0.05）,且蓝光组T3、T4和睾酮水平比其他光组高14.36%-26.20%。结果提示蓝光比其他单色光更能促进甲状腺激素和睾酮的分泌,进而影响肉鸡睾丸的形态结构。 相似文献
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Testosterone concentrations in the semen plasma of the cocks of the laying type were analyzed. The levels in semen plasma were substantially lower than in peripheral blood and were found at the interval of 1.58 to 13.30 nmol . 1(-1), the average values amounted to 5.5 nmol . 1(-1). At the repeated samplings of the same animals, considerable variability of hormone levels was observed, which suggested a possibility of the pulsation character of the changes in the androgen also in this biological material. The correlation coefficients between the testosterone levels in semen plasma and ejaculate volume, as well as between the sperm concentration were positive, however, statistically insignificant. The relation between testosterone and the weight of testes was at the significance limit. 相似文献
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Danikowski S Sallmann HP Halle I Flachowsky G 《Journal of animal physiology and animal nutrition》2002,86(11-12):376-382
This study was an attempt to find whether the reproductive performance of cocks would be influenced by oral administration of different amounts of dietary vitamin E over a long period of time. For that purpose 60 cocks were divided into five dietary groups of 12 animals each, and supplemented with 0 (control group), 100, 1,000, 10,000 or 20,000 IU alpha-tocopherol/kg diet, respectively, over a period of 12 months. The effect on semen parameters and biochemical parameters measured in pooled semen samples and the weight of the testes were determined. The weight of testes decreased with increasing amounts of supplemented vitamin E. Volume, pH, colour, consistency and motility were not influenced by the diets, but density of ejaculate (sperm/microl), total amount of spermatozoa and morphology of sperm were significantly lowered by increasing amounts of supplemented vitamin E. The alpha-tocopherol concentration in ejaculates increased significantly in relation to the diet whereas phospholipid content and thiobarbituric acid-reactive substances (TBARS) of the semen samples decreased significantly with increasing vitamin E supplementation. The reproductive performance of cocks was negatively influenced by high doses of vitamin E although decreased TBARS indicated rising oxidative defence. 相似文献
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Testosterone concentration in the seminal plasma of cocks 总被引：1，自引：0，他引：1
Testosterone concentrations in the seminal plasma of cocks ranged from 0.46 to 5.05 ng/ml and were substantially lower than in blood plasma. No significant variation was noted in seminal plasma testosterone concentrations during the light phase of the day, whereas the concentration in blood declined over this period. Spermatozoal concentration and seminal testosterone decreased in the third sample of the semen collected sequentially at 3 h intervals. Testosterone concentrations in seminal plasma (1.57 +/- 0.17 ng/ml) and in the semen from the ductus deferens (1.34 +/- 0.24 ng/ml) were not significantly different. 相似文献
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种公鸡日粮中添加蜂花粉的效用研究 总被引：3，自引：0，他引：3
研究蜂花粉对人工采精中采不出精液的公鸡的繁殖性能及内分泌机能的影响。结果表明:日粮中添加2%的蜂花粉,能显著改善公鸡的精液量、精子活力、精子密度和有效精子数(P<0.05),对精子畸形率影响不大(P>0.05),有效缓解血浆T水平的下降(P<0.05),对E2水平无明显影响;与对照组相比,添加蜂花粉后试验组血浆T3含量升高(P<0.05)、T4含量(P<0.05)和皮质醇含量(P>0.05)下降,说明蜂花粉改善了热应激公鸡的甲状腺功能和肾上腺功能。 相似文献
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2 groups of 20 cocks each were selected at random from non-dwarf White Leghorn (28 weeks post-hatch) and dwarf Krishna-J (38 weeks post-hatch) genotypes. The treated groups comprised 10 White Leghorn and 10 Krishna-J cocks. The remaining birds served as controls. 8 weeks prior to furazolidone treatment, semen was collected from both control groups at regular 4-day intervals, for 4 weeks. Cocks of the treated groups of both genotypes were administered furazolidone (0.14 g/bird/day) for 7 consecutive days. Semen was collected from all cocks at regular 4-day intervals for 4 weeks. Semen from the cocks of the same group was pooled. The pooled ejaculate volume and sperm density did not differ significantly in the 2 genotypes. The semen output as well as sperm density increased along with progressive attainment of sexual maturity. Furazolidone treatment caused significant reduction in semen volume as well as sperm concentration in either genotype. 相似文献
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This study was conducted to determine the pathological changes in testes and epididymides and plasma testosterone levels of adult roosters during experimentally induced aflatoxicosis. In the study, 24 months of age, 32 Babcock breeder males were used, and they were divided into four groups each containing 8 animals. The groups were designed as follows; group 1: Control, no aflatoxin (AF), group 2: 5 ppm (parts per million) total aflatoxin (AF; B1, B2, G1, G2), group 3: 10 ppm AF and group 4: 20 ppm AF in the diet, and the birds were fed for 8 weeks. Grossly, it was seen that the testes of all AF-treatment groups birds were significantly (P < 0.001) atrophied when compared with those of control birds. Histopathologically, there was no spermatogenesis in the testes of 4, 5 and 6 cocks fed on a diet containing AF 5, 10 and 20 ppm, respectively. Furthermore, abnormal spermatozoa were observed in some of AF-treatment groups (in 2 cases in each of 5 and 10 ppm AF-treated groups, and in one case in 20 ppm AF-treated group). There were also mononuclear cell infiltration and/or focal lymphoid cell accumulation in the intertubular areas of the testes and epididymides in all AF-treatment groups. In conclusion, it has been shown that AF might totally or partially (dose related) suppress spermatogenesis, cause abnormality in spermatozoa and atrophy in testes. Furthermore, there was degeneration and desquamation in the epithelium and decrease in the size and thickness of the germinative layer of the seminiferous tubules, and lowered plasma testosterone levels in adult roosters. 相似文献
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Phenotypic Correlations of Testes Size with Semen Traits and the Productive Traits of Young Boars 
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下载免费PDF全文 The aim of this study was to investigate whether there is a relationship of young boar testes size with semen traits and with productive traits. The dimensions (length, width and volume) of each testis and semen traits (semen volume, percentage of sperm with progressive motility, sperm concentration, total number of sperm in semen, percentage of sperm with normal acrosome, percentage of sperm with major and minor morphological defects, osmotic resistance test value and activity of aspartate aminotransferase in seminal plasma) were determined on 120 young boars aged 6 months. At 180 day of age, the boars backfat thickness and leanness (by ultrasonic apparatus) and body weight were also measured. The average daily gain was determined in the period from 70 to 180 days of age of the boars. The results showed that the sperm concentration, total number of sperm in semen and percentage of progressive motile sperm were a significantly positively correlated with width and volume of the left (p ≤ 0.01) and right testis (p ≤ 0.05) and with total volume of both testes (p ≤ 0.01). But the highest values of correlation coefficients were found between the width of the left testis and sperm concentration, total number of sperm in semen and percentage of progressive motile sperm. A correlations of dimensions (length and width) and volume of testes with other semen traits were very low and statistically non‐significant. The volume of testes (left and right testis and total testes) was significantly positively correlated with body weight at 180 days of age and daily gain (p ≤ 0.01), but lower correlation coefficient was between left testis and daily gain (p ≤ 0.05), whereas correlations were low and non‐significant with leanness and backfat thickness. 相似文献
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本文用Jaswant酶测定法和组织学方法就日粮锰对公鸡琥珀酸脱氢酶(SDH)活性及睾丸曲细精管中精子密度的影响进行了研究。结果表明,缺锰的Ⅰ组(添加Mn 0ppm)或高锰的Ⅳ组(添加Mn 300ppm)致使血清、肝脏和睾丸SDH活性下降。组织学观察表明:Ⅰ、Ⅳ组与Ⅲ组(添加Mn 100ppm)相比性成熟延迟,睾丸曲细精管精子数降低(P<0.01)。睾丸SDH 活性与曲细精管精子数相关系数r-0.9493,提示日粮中锰缺乏或过多造成曲细精管精子生成下降的重要原因之一是由于锰影响了睾丸SDH活性。 相似文献
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Birds obtained by embryo engineering are used to study embryo development and to produce transgenic birds. As this method of producing birds still generate strong emotions of the public opinion head ornaments, testes and semen characteristics of sex chimera roosters were examined to check whether they differ from chickens obtained by non‐manipulated methods. Measurements of head ornaments, testes and semen were correlated with each other. Semen quality factor (SQF) was calculated, as well as the level of fluctuating asymmetry (FA) of bilateral traits (wattles and testes). Positive correlation was found for comb width and wattle length and comb thickness and sperm concentration. Semen characteristics and FA did not exceed the level encounter in other chicken lines. Results obtained indicate that germline chimeras are similar in appearance of secondary sexual traits, and semen and testes characteristics to chickens produced in non‐manipulated way. 相似文献
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Alghamdi AS Troedsson MH Xue JL Crabo BG 《American journal of veterinary research》2002,63(6):880-885
OBJECTIVE: To compare the effect of semen extender and seminal plasma on postthaw motility and filtration through a glass wool-Sephadex (GWS) filter for frozen stallion semen. SAMPLE POPULATION: 7 stallions from which we collected > or = 3 ejaculates/stallion. PROCEDURES: 4 experiments were conducted to evaluate postthaw quality of frozen stallion semen. Kenney extender was compared with glucose-EDTA extender by use of various dilution rates that resulted in differing concentrations of seminal plasma. Stallions known to produce semen with poor postthaw quality were used to investigate whether a particular extender or dilution rate could improve ability of such semen to survive freeze-thaw procedures. RESULTS: Use of Kenney extender as the centrifugation extender significantly improved postthaw motility and GWS filtration, compared with glucose-EDTA. Extending semen at a dilution of 1:3 was significantly better than 1:1 for both motility and GWS filtration. In addition, including seminal plasma at a concentration of 5% in the cryopreserved semen resulted in significantly higher yield of spermatozoa after GWS filtration, compared with complete removal of SP or use of seminal plasma at 25%. Lastly, semen with poor postthaw quality had significantly improved postthaw quality in regard to motility and GWS filtration when semen was frozen with seminal plasma at a concentration of 5%, compared with semen frozen with seminal plasma at a concentration of 25%. CONCLUSIONS AND CLINICAL RELEVANCE: Use of Kenney extender at a high dilution (> or = 1:3) immediately after collection of semen can improve postthaw quality of frozen stallion semen. 相似文献