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Fourteen developed microsatellite markers were characterized for their use in genotyping and diversity studies of olive varieties.
After optimisation of microsatellite assay and allele sizing, ninety-six alleles were found in nineteen varieties, with an
average of 6.8 alleles per locus. The characteristics of the microsatellite markers were used to identify markers that can
be reliably applied for variety genotyping. Such features were the generation of complex banding patterns supported by underlying
allele sequences, `short allele dominance', an unstable repeat structure and a low number of alleles. AFLP analysis was performed
on the same set of olive varieties using eight primer pair combinations. The genetic relationships among nineteen olive varieties
were compared on the basis of microsatellite and AFLP polymorphisms. Genetic distances between all pairwise combinations of
the varieties were calculated using Jaccard's coefficient of similarity and dendrograms were constructed by the UPGMA method.
The results of clustering analysis with both molecular systems showed the common genetic background of Tuscan varieties, and
genetic divergence within Slovene olive germplasm. Slovenian varieties ‘Buga’, ‘Štorta’ and ‘Samo’ might represent regionally
selected olives, while ‘Zelenjak’ and ‘Črnica’ are probably derived from the Central Italian region. The predominant local
‘Istrska belica’ was introduced to Slovenia independently from the other regional varieties and showed the lowest genetic
similarity with the other regional varieties.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
3.
Sequence-related amplified polymorphism (SRAP), simple sequence repeats (SSR), inter-simple sequence repeat (ISSR), peroxidase
gene polymorphism (POGP), resistant gene analog (RGA), randomly amplified polymorphic DNA (RAPD), and a morphological marker,
Alternaria brown spot resistance gene of citrus named as Cabsr caused by (Alternaria alternata f. sp. Citri) were used to establish genetic linkage map of citrus using a population of 164 F1 individuals derived between ‘Clementine’ mandarin (Citrus reticulata Blanco ‘Clementine) and ‘Orlando’ tangelo’ (C. paradisi Macf. ‘Duncan’ × C. reticulata Blanco ‘Dancy’). A total of 609 markers, including 385 SRAP, 97 RAPD, 95 SSR, 18 ISSR, 12 POGP, and 2 RGA markers were used
in linkage analysis. The ‘Clementine’ linkage map has 215 markers, comprising 144 testcross and 71 intercross markers placed
in nine linkage groups. The ‘Clementine’ linkage map covered 858 cM with and average map distance of 3.5 cM between adjacent
markers. The ‘Orlando’ linkage map has 189 markers, comprising 126 testcross and 61 intercross markers placed in nine linkage
groups. The ‘Orlando’ linkage map covered 886 cM with an average map distance of 3.9 cM between adjacent markers. Segregation
ratios for Cabsr were not significantly different from 1:1, suggesting that this trait is controlled by a single locus. This locus was placed
in ‘Orlando’ linkage group 1. The new map has an improved distribution of markers along the linkage groups with fewer gaps.
Combining different marker systems in linkage mapping studies may give better genome coverage due to their chromosomal target
site differences, therefore fewer gaps in linkage groups. 相似文献
4.
Jonathan Mkumbira Linley Chiwona-Karltun Ulf Lagercrantz Nzola Meso Mahungu John Saka Albert Mhone Mpoko Bokanga Leon Brimer Urban Gullberg Hans Rosling 《Euphytica》2003,132(1):7-22
Cassava roots, a major food in Africa, contain cyanogenic glucosides that may cause toxic effects. Malawian women farmers
considered fields of seemingly similar cassava plants to be mixes of both ‘cool’ and ‘bitter’ cultivars. They regard roots
from ‘cool’ cultivars as non-toxic. Roots of ‘bitter’ were considered to require extensive traditional processing done by
women to be safe for consumption. But curiously, these women farmers preferred ‘bitter’ cultivars since toxicity confers protection
against theft, which was a serious threat to the food security of their families. We studied how well these farmers comprehend
the effects of genetic variations in cassava when dealing with cyanogenesis in this complex system. Using molecular markers
we show that most plants farmers identified as belonging to a particular named cultivar had a genotype typical of that cultivar.
Farmers' ethno-classification into ‘cool’ and ‘bitter’ cultivars corresponded to a genetic sub-division of the typical genotypes
of the most common cultivars, with four-fold higher cyanogenic glucoside levels in the bitter cultivars. Examining morphology,
farmers distinguished genotypes better than did the investigators when using a standard botanical key. Undoubtedly, these
women farmers grasp sufficiently the genetic diversity of cassava with regard to cyanogenesis to simultaneously benefit from
it and avoid its dangers. Consequently, acyanogenic cassava – the breeding of which is an announced good of some cassava genetic
improvement programmes – is not a priority to these farmers. Advances in molecular genetics can help improve food supply in
Africa by rapid micropropagation, marker assisted breeding and introduction of transgenic varieties, but can also help to
elucidate tropical small-scale farmers' needs and skills.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
5.
Ioannis V. Ganopoulos Konstantinos Kazantzis Ioannis Chatzicharisis Irene Karayiannis Athanasios S. Tsaftaris 《Euphytica》2011,181(2):237-251
It is important to couple phenotypic analysis with genetic diversity for germplasm conservation in gene bank collections.
The use of molecular markers supports the study of genetic marker-trait associations of biological and agronomic interest
on diverse genetic material. In this report, 19 Greek traditional sweet cherry cultivars and two international cultivars,
which were used as controls, were grown in Greece and characterized for 17 morpho-physiological traits, 15 simple sequence
repeat (SSR) loci and 10 inter simple sequence repeat (ISSR) markers. To our knowledge, this is the first report on molecular
genetic diversity studies in sweet cherry in Greece. Principal component analysis (PCA) of nine qualitative and eight quantitative
morphological parameters explain over 77.33% of total variability in the first five axes. The SSR markers yielded a combined
matching probability ratio (MPR) of 9.569 × e−12. The 15 SSR loci produced a total of 92 alleles. Ten ISSR primers generated
91 bands, with an average of 9.1 bands per primer. Expected heterozygosity (gene diversity) values of 15 SSR loci and 10 ISSR
markers averaged at 0.683 and 0.369, respectively. Based on stepwise multiple regression analysis (MRA), SSR alleles were
found associated with harvest time and fruit polar diameter. Furthermore, three ISSR markers were correlated with fruit harvest
and soluble solids and four ISSR markers were correlated with fruit skin color. Stepwise MRA identified six SSR alleles associated
with harvest time with a high correlation (P < 0.001), with linear associations with high F values. Hence, data analyzed by the use of MRA could be useful in marker-assisted breeding programs when no other genetic
information is available. 相似文献
6.
Rosa Karla Nogueira Pestanana Edson Perito Amorim Cláudia Fortes Ferreira Vanusia Batista de Oliveira Amorim Larissa Santos Oliveira Carlos Alberto da Silva Ledo Sebastião de Oliveira e Silva 《Euphytica》2011,178(2):151-158
Bananas are tropical fruits grown worldwide playing a key role in market trade and especially used as main food source for
low income populations. In Brazil, bananas are mainly consumed in natura, occupying the second largest internal market. Nevertheless, this crop presents low availability of productive commercial
varieties with good agronomic characteristics. A strategy undertaken to solve this problem is the development of new cultivars
through conventional genetic breeding methods. However, this strategy presents some obstacles such as female sterility and
low number of seeds. In order to overcome these shortcomings, use of mutation induction aiming the selection of mutants with
desirable agronomic characteristics seems to have great potential for developing new cultivars. The objective of the present
work was to evaluate the genetic variability in putative banana ‘Pacovan’ (AAB genome, subgroup Prata Type) mutants submitted
to gamma ray irradiation, using a set of agronomical and molecular data (ISSR markers). The distance between the putative
‘Pacovan’ mutants varied from 0.26 to 0.64 with cophenetic correlation coefficient of 0.7669. Four mutants were selected based
on best agronomical characteristics and height. This data also shows that there is variability that can be explored after
the irradiation of ‘Pacovan’ banana mutants, which can be used in the genetic breeding program of banana aiming to develop
short new varieties that also present good agronomic characteristics. This is the first attempt to use combined data in order
to evaluate the genetic variability in putative banana mutants. 相似文献
7.
Bhawna Saxena Rajinder Kaur Satya Vrat Bhardwaj 《Journal of Crop Science and Biotechnology》2011,14(3):191-196
Genetic relationship and diversity among seven cabbage cultivars were analyzed using RAPD and SSR markers. These cultivars
are of great commercial value in India and are confirmed for their reaction to black rot caused by Xanthomonas campestris pv. campestris. However, so far the extent of genetic diversity and relatedness has not been studied in these cultivars. A total of 17 selected
RAPD primers generated 90 bands, 76 of which were polymorphic (84.44%). In addition, 27 selected SSR primers generated 67
amplified bands with 59 of which were polymorphic (87.6%). Though both the marker techniques were able to discriminate the
cultivars effectively, analysis of combined data of markers (RAPD and SSR) resulted in better distinction of cultivars. By
combining both the markers, a total of 157 bands were detected of which 135 bands (85.98%) were polymorphic, i.e. an average
of 5.95 bands per primer. High level of polymorphism (> 85%) recorded with two different marker systems indicated a high level
of genetic variation existing among the cultivars. Genetic relationship estimated using similarity co-efficient (Jaccard’s)
values between different pairs of cultivars varied from 0.21 to 0.77 in RAPD, 0.42 to 0.82 in SSR, and 0.43 to 0.89 with combined
markers. A high correspondence had been recorded between the values of genetic variations generated by UPGMA, clustering,
and scatter plot diagrams. The cultivars ‘January King Sel. Improved’ and ‘Golden Acre’ are highly divergent cultivars as
demonstrated by both the marker systems. 相似文献
8.
应用SSR和ISSR标记分析栽培香稻品种的遗传多样性 总被引：27，自引：0，他引：27
本研究利用24对SSR引物和36个ISSR引物,分析33份来源于亚洲10个国家的香稻品种的遗传多样性。分别获得93条和181条多态性片段,每个SSR座位可检测3～8个等位基因,平均为4．23个;每个ISSR引物可检测3～8个多态性位点,平均为5．03个。根据SSR和ISSR标记计算的品种间遗传相似系数分别在0．294～0．884之间和0．595～0．867之间。聚类分析表明,利用两种标记所得的聚类结果基本上一致,与品种所处的3种气候类型变化基本相符。进一步证实SSR和ISSR标记是研究水稻种质资源分类有效的工具。 相似文献
9.
Takehiko Shimada Hiroko Hayama Takashi Haji Masami Yamaguchi Masao Yoshida 《Euphytica》1999,109(3):143-147
We investigated the genetic diversity of 42 plum varieties by RAPD analysis. Twenty primers discriminated all plum varieties
excepting two synonymous pairs: 'Botankyou and ‘Kelsey’, and ‘Chairn’ and ‘Tragedy’. Two North American plums, ‘Beach Plum’
and ‘Glow’, were genetically distinct from the other plums by cluster analysis. Overlaps observed between the ‘European plum
group’ and the ‘Japanese plum group’, were perhaps due to intercrossing. We could also discriminate ‘Sordum’ from 'Late Sordum
and ‘Bansei Sordum’, although ‘Late Sordum’ and ‘Bansei Sordum’ are thought to be derived from bud mutants of ‘Sordum’.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
新疆自育陆地棉品种SSR遗传多样性分析 总被引：7，自引：2，他引：5
利用SSR标记对94份新疆自育陆地棉品种的基因组进行分析,研究新疆陆地棉品种的遗传多样性。结果: 从分布于棉花全基因组的206对SSR标记中筛选出54对具有稳定多态性的引物, 共检测出153个多态性位点, 每对引物的等位变异为2~6个,平均为2.93个;基因型多样性(H′)变幅为0.0439–0.7149,平均为0.4491;引物多态信息含量(PIC)为0.0430–0.6640,平均为0.3831。表明SSR标记在品种间可以反映较丰富的遗传多样性信息。94份品种间成对遗传相似系数变幅为0.3846~0.9835, 71.9%的品种相似系数在0.601~0.800内,反映出新疆陆地棉品种间的遗传相似性相对较高。根据UPGMA 聚类分析,在阈值为0.63时,将94份品种划分为2个类群,说明新疆陆地棉品种间遗传关系相对简单,品种的遗传基础相对狭窄,品种遗传组分差异较小,总体上遗传多样性不够丰富;分子聚类结果与品种本身遗传系谱背景和演变趋势吻合度较高,符合品种的真实特性。研究证明,自育品种在分子水平上差异不大,需要努力拓宽品种选育的遗传基础。 相似文献
11.
Manli Li Nana Yuyama Mariko Hirata Jianguo Han Yunwen Wang Hongwei Cai 《Euphytica》2009,170(3):327-338
A consensus genetic linkage map with 447 SSR markers was constructed for zoysiagrass (Zoysia japonica Steud.), using 86 F1 individuals from the cross ‘Muroran 2’ × ‘Tawarayama Kita 1’. The consensus map identified 22 linkage groups and had a total
length of 2,009.9 cM, with an average map density of 4.8 cM. When compared with a previous AFLP-SSR linkage map, the SSR markers
from each linkage group mapped to similar positions in both maps. Eight pairs of linkage groups from the AFLP-SSR map were
joined into eight new groups in the current map. This zoysiagrass consensus map contained 35 SSR markers exhibiting high homology
with rice genomic sequences from known chromosomal locations. This allowed synteny to be identified between Zoysiagrass linkage
groups 2, 3, 9, 19 and rice chromosomes 3, 12, 2, 7 respectively. These results provide important comparative genomics information
and the new map is now available for quantitative trait locus analysis, marker-assisted selection and breeding for important
traits in zoysiagrass. 相似文献
12.
N. F. Weeden M. Hemmatt D. M. Lawson M. Lodhi R. L. Bell A. G. Manganaris B. I. Reischs S. K. Brown G. -N. Ye 《Euphytica》1994,77(1-2):71-75
Summary Extensive linkage maps, consisting primarily of molecular markers, are being developed for apple, pear, and grape varieties.
The intrinsically high heterozygosity of outcrossing perennial species is utilized to produce segregating populations directly
from a cross between varieties. Nearly complete linkage maps have been generated for the apple varieties ‘Rome Beauty’ and
‘White Angel’. The map for ‘Rome Beauty’ contains 161 molecular markers, while that for ‘White Angel’ has 251 markers. Maps
for the pear varieties, ‘Bartlett’ and NY10353, also are being developed. Linkages conserved between the pear and apple genomes
have been identified. In grapes, maps for four varieties are available, the most extensive being those for ‘Cayuga White’
and ‘Aurore’. The apple maps have been used to investigate the genetic basis of morphological and physiological characters.
A gene controlling the presence of anthocyanins in the skin of the fruit is located on linkage group 3. Genes controlling
early bud break, branching habit, and production of root suckers have also been identified and mapped. 相似文献
13.
Sergio Pietro Ferrante Sergio Lucretti Silvia Reale Alessandro De Patrizio Loredana Abbate Nicasio Tusa Maria-Teresa Scarano 《Euphytica》2010,173(2):223-233
We report the accurate determination of the allelic configurations of a total of eight new citrus tetraploid hybrids by means
of SSR analysis, coupled with capillary electrophoresis, and PCR based dosage effects. Tetraploid hybrids were spontaneously
obtained from different interploid crosses (2x × 4x) between diploid ‘Femminello’ lemon and the allotetraploid somatic hybrid
(2n = 4x = 36) ‘Key’ lime + ‘Valencia’ orange, and between diploid ‘Wilking’ and ‘Fortune’ mandarins and an autotetraploid ‘Dancy’
mandarin (2n = 4x = 36). To understand the opportunity to employ them in further backcross programs, the cytological mechanisms underlying
their ploidy level were unambiguously determined using six SSR primers. PCR conditions were optimized and skewness in template/product
ratios were verified. Tetraploid allelic configurations were determined from PCR based dosage effects using electropherogram
peak heights to estimate the copy number per allele. In all the tetraploid hybrids we found out that diploginy (2n eggs) has occurred, contributing the extra haploid genome in the tetraploids. According to the marker genotypes, it was further
inferred that the 2n eggs in ‘Femminello’ lemon resulted from first division restitution (FDR), while in ‘Wilking’ and ‘Fortune’ mandarins 2n eggs occurred in second division restitution (SDR). These new genotypes, with their improved genetic female background, can
be therefore considered very valuable in our citrus genetic improvement program as pollen donors in backcrosses suitable to
eliminate negative traits. 相似文献
14.
Lei Zhang Zuozhou Li Yanchang Wang Zhengwang Jiang Shengmei Wang Hongwen Huang 《Euphytica》2010,175(1):133-143
Seedlings derived from an Actinidia interspecific cross between the hexaploid Actinidia chinensis var. deliciosa ‘Jinkui’ and the diploid male A. eriantha × A. chinensis var. chinensis ‘Chaohong’ hybrid were analyzed using flow cytometry, SSR markers and phenotypic observations. The results show that the
leaf vitamin C content of this hybrid population has a mid-parent heterosis. Separation of flower color in the progeny was
also observed, progeny with red flowers lighter than ‘Chaohong’, white flowers as in ‘Jinkui’ and intermediate types with
a red base to the petals and white margins were all present. Flow cytometry analysis confirmed that most of the progeny were
tetraploids, and molecular marker data showed that most of these tetraploid progeny had three alleles from the hexaploid parent
and one allele from the diploid parent. UPGMA analysis based on the SSR markers showed that the diploid parent was completely
separated from the hexaploid parent and all the progeny. 相似文献
15.
Vipin Kumar Shailendra Sharma Amit Kumar Sharma Shiveta Sharma K. Venkataramana Bhat 《Euphytica》2009,170(3):249-262
Morpho-agronomic traits and microsatellite markers were used to survey genetic diversity in 115 common bean genotypes that
included 70 Indian landraces, 24 released varieties and 21 exotic accessions. Twelve morpho-agronomic traits, namely, days
to 50% flowering, leaflet length, leaflet width, pod length, pod width, number of pods per plant, days to maturity, seed length,
seed width, number of seeds per pod, 100 seed weight and seed yield per plant were studied. Field data of two consecutive
years were subjected to multivariate analysis as proposed by Mahalanobis’s D2-statistics, Tochers method of clustering and combined analysis of variance. Seventeen microsatellite markers were also used
to examine genetic diversity at molecular level that showed polymorphic information content (PIC) in the range of 0.00–0.684.
Dendrograms based on Euclidean distances and UPGMA analysis showed the presence of majority of released varieties into single
cluster, which pointed toward their low genetic base in comparison to indigenous landraces and exotic germplasm. Significant
correlation existed between morphological genetic distance and microsatellite genetic distance tested by Mantel test (r = 0.876). 相似文献
16.
D. Kaemmer D. Fischer R.L. Jarret F.-C. Baurens A. Grapin D. Dambier J.-L. Noyer C. Lanaud G. Kahl P.J.L. Lagoda 《Euphytica》1997,96(1):49-63
Musa species are among the tallest monocotyledons and include major food-producing species. The principal cultivars, derived
from two major species Musa acuminata (‘A’ genome) and Musa balbisiana (‘B’ genome), are polyploid hybrids (mainly AAA, AAB
and ABB triploids), medium to highly sterile, parthenocarpic and clonally propagated. Bananas and plantains are crops to which
molecular breeding is expected to have a positive impact. In order to better understand banana genetics, more knowledge has
to be accumulated about the complex genome structure of hybrids and cultivars. Therefore, the aim of our work is to develop
molecular markers that are codominant, reliable, universal, highly polymorphic and that are applicable to collaborative Musa
germplasm genotyping and mapping. Two size-selected genomic libraries have been screened for the presence of simple sequence
repeats (SSR). Our data demonstrate that SSR are readily applicable to the study of Musa genetics. Our comprehensive analyses
of a significant number of banana sequence tagged microsatellite sites (STMS) will add to our knowledge on the structure and
phylogeny of genomes of the Musa species, and suggest that microsatellites be used as anchor markers for a banana genetic
core map. Additional markers, such as e.g. CAPS have also been tested in order to increase the detection of polymorphisms
exceeding that revealed by STMS technology. The utility of PCR-derived markers for collaborative genetic analyses of the banana
genome, and the transferability of 'streamlined’ laboratory techniques and data analysis to Developing Countries are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
17.
Harsukh Popatbhai Gajera Rinkal Kishorbhai Domadiya Sunil Vrajlal Patel Baljibhai Arjanbhai Golakiya 《Journal of Crop Science and Biotechnology》2014,17(2):79-88
The genetic variability and relationships among 11 cowpea genotypes representing two cultivars and nine elite genotypes were analyzed using 22 random amplified polymorphic DNA (RAPD) and nine inter-simple sequence repeat (ISSR) markers. ISSR markers were more efficient than RAPD assay with regards to polymorphism detection. But the average numbers of polymorphic loci per primer and resolution power were found to be higher for RAPD than for ISSR. Also, the total number of genotype specific marker loci, Nei’s genetic diversity, Shannon’s information index, total heterozygosity, and average heterozygosity were prominent in RAPD as compared to ISSR markers. The regression test between the two Nei’s genetic diversity indices showed low regression (0.3733) between ISSR and RAPD + ISSR-based similarities but maximum (0.9823) for RAPD and RAPD + ISSR-based similarities. The RAPD- and ISSR-generated cultivar- or genotype-specific unique DNA fingerprints able to identify the most diverse genotypes. A dendrogram constructed based on RAPD and ISSR combined data indicated a very clear pattern of clustering according to the groups (cultivars and elite genotypes). The results of principal coordinate analysis were comparable to the cluster analysis. Cluster analysis showed that most diverse genotypes (GP-125 — small size with good seed quality; GP-129, GP-90L — big size with poor seed quality) were separated from moderately diverse cultivars and genotypes. The genetic closeness among GP-129 and GP-90L, JCPL-42, and JCPL-107 could be explained by the high degree of commonness in these genotypes. 相似文献
18.
Quantitative analysis and QTL mapping for agronomic and fiber traits in an RI population of upland cotton 总被引：3，自引：1，他引：2
Jixiang Wu Osman Ariel Gutierrez Johnie N. Jenkins Jack C. McCarty Jun Zhu 《Euphytica》2009,165(2):231-245
Genetic mapping is an essential tool for cotton (Gossypium hirsutum L.) molecular breeding and application of DNA markers for cotton improvement. In this present study, we evaluated an RI population
including 188 RI lines developed from 94 F2-derived families and their two parental lines, ‘HS 46’ and ‘MARCABUCAG8US-1-88’, at Mississippi State, MS, for two years.
Fourteen agronomic and fiber traits were measured. One hundred forty one (141) polymorphic SSR markers were screened for this
population and 125 markers were used to construct a linkage map. Twenty six linkage groups were constructed, covering 125
SSR loci and 965 cM of overall map distance. Twenty four linkage groups (115 SSR loci) were assigned to specific chromosomes.
Quantitative genetic analysis showed that the genotypic effects accounted for more than 20% of the phenotypic variation for
all traits except fiber perimeter (18%). Fifty six QTLs (LOD > 3.0) associated with 14 agronomic and fiber traits were located
on 17 chromosomes. One QTL associated with fiber elongation was located on linkage group LGU01. Nine chromosomes in sub-A
genome harbored 27 QTLs with 10 associated with agronomic traits and 17 with fiber traits. Eight chromosomes in D sub-genome
harbored 29 QTLs with 13 associated with agronomic traits and 16 with fiber traits. Chromosomes 3, 5, 12, 13, 14, 16, 20,
and 26 harbor important QTLs for both yield and fiber quality compared to other chromosomes. Since this RI population was
developed from an intraspecific cross within upland cotton, these QTLs should be useful for marker assisted selection for
improving breeding efficiency in cotton line development.
Paper number J1116 of the Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Mississippi
State, MS 39762. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product
by USDA, ARS and does not imply its approval to the exclusion of other products or vendors that may also be suitable. 相似文献
19.
Kamal Foud Abdellatif Yehia Abdallah Khidr 《Journal of Crop Science and Biotechnology》2010,13(3):139-145
Genetic diversity of four new yellow single crosses, five new yellow three-way crosses, and five yellow inbred lines of maize
(Zea mays L.) was studied using different molecular (SSR, ISSR, and RAPD) and biochemical markers (seed storage protein content). All
markers were able to clearly separate the inbred lines in one cluster from the different types of hybrids. The correlation
among the different types of molecular markers was moderately high according to the Mantel’s test (e.g. 0.67 between SSR and
ISSR, 0.42 between SSR and RAPD, and 0.65 between ISSR and RAPD), indicating that the three techniques are efficient for evaluating
genetic diversity in the maize genotypes. The correlation of biochemical markers (seed storage protein SDS-PAGE) with SSR,
ISSR, and RAPD markers was 0.61, 0.74, and 0.48, respectively. It can be concluded that both molecular and biochemical markers
are efficient to study the genetic diversity in maize. Among the different types of molecular markers, SSR is a more accurate
marker-type because of its co-dominance and stability of results. It can also be said that biochemical and molecular markers
are positively correlated and the correlation ranged between moderate to high. This could suggest using both marker types,
separately or together, for genetic diversity studies in maize. 相似文献
20.
Rice blast resistance gene ‘Pi-z’ present in rice genotypes, Zenith and Fukunishiki, represents a potential source of blast resistance for the north-western
Himalayan region of India. We tested the reliability of microsatellite markers linked to Pi-z for assessing blast resistance phenotype in crosses of commercial importance. A new set of microsatellite markers linked
to Pi-z was also developed by exploiting the publicly available marker and genomic resources of rice. Of the three previously reported
markers for Pi-z, only MRG5836 was suitable for the marker assisted selection of Pi-z. Among the 17 microsatellites selected from the putative region of Pi-z locus, two, RM8225 and RM8226 cosegregated with MRG5836 and were located at distance of 1.2–4.5 cM from the gene. A new microsatellite marker ‘SSR236’ was developed from the (CT)16 repeat of PAC clone P0502B12, which exhibited closer linkage (0.6–1.2 cM) to Pi-z. Survey of the allelic diversity at the loci of the Pi-z linked microsatellite markers revealed that the Fukunishiki and Zenith type alleles were not present in majority of the local
indica rice genotypes. As these markers are polymorphic between the Pi-z donors and a great majority of local indica rices tested, they can be used as a selection tool in rice breeding programs aimed at improving the blast resistance of local
rices. 相似文献