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1.
抗球虫新药癸氧喹酯在鸡体内的残留试验 总被引：1，自引：0，他引：1
建立了高效液相色谱-荧光法(HPLC-FLD)来检测肉鸡肌肉、肝脏、肾脏和皮肤/脂肪组织中癸氧喹酯的残留量。将癸氧喹酯以0.05,0.2,0.6μg/g的含量分别添加到空白组织中,测得肌肉、肝脏、肾脏和皮肤/脂肪组织的回收率大于75%,变异系数低于9%。用该方法测定肌肉、肝脏、肾脏和皮肤/脂肪组织中癸氧喹酯的最低检测限为0.01μg/g。试验鸡以30mg/L的癸氧喹酯的剂量连续7d饮水给药,停药后各组织中癸氧喹酯的残留量逐渐下降;停药第3天后肌肉中检测不到癸氧喹酯,停药后第5天所有组织中均检测不到残留药物。 相似文献
2.
高效液相色谱法测定猪血浆及组织中喹赛多及其脱二氧代谢物 总被引：1，自引：0，他引：1
建立了测定猪血浆及肝脏、肾脏、肌肉等组织中的喹赛多及其代谢物脱二氧喹赛多的高效液相色谱(HPLC)法.血浆样用甲醇沉淀蛋白后离心取上清液进样测定.组织样先用乙腈匀浆,用正己烷脱脂后作HPLC检测.色谱柱为ODS C18柱;流动相血浆样测定为乙腈水(2080),组织样测定为甲醇水(4258),流速为1.0 mL/min;紫外检测波长305 nm.药物工作液质量浓度范围为0.005～0.500 mg/L时,血浆样品及组织样品测定条件下药物质量浓度与响应值均具良好线性关系,相关系数＞0.999.血浆中药物质量浓度为0.02、0.10、0.50 mg/L时,喹赛多及脱二氧喹赛多的回收率均大于70%,组织中药物含量为0.05、0.20、1.00μg/g时,肌肉样品的回收率均大于70%,肝脏、肾脏样品则为50%～80%.本试验条件下,喹赛多及其脱二氧代谢物的最低检出质量浓度,血浆样品分别为0.01、0.02mg/L,组织样品2种检测物均为0.025μg/g.测定了工作液3种质量浓度0.01、0.05、0.25 mg/L的仪器精密度,日内相对偏差＜8.0%,日间相对偏差＜17.0%. 相似文献
3.
建立了鸡组织(肌肉、肝脏、皮肤/脂肪)中马杜霉素残留的高效液相色谱荧光检测法。肌肉、肝脏样品经异辛烷提取,皮肤/脂肪样品经乙腈提取,Florisil固相萃取柱净化,丹磺酰肼衍生后上机测定。采用Supelco C18色谱柱分离,以乙腈-7 mmol/L硫酸氢化四丁胺溶液(85∶15,V/V)为流动相,荧光检测器检测,激发波长为260 nm,发射波长为510 nm。结果显示,在0.15~7.2μg/mL浓度范围内,线性关系良好;组织添加回收率均大于70%,定量限为0.03 mg/kg。 相似文献
4.
《中国家禽》2020,(7)
为建立一种高效液相色谱-串联质谱法检测鸡蛋和鸡组织(肝脏、肾脏、肌肉、脂肪)中恩诺沙星、环丙沙星的残留,鸡蛋和鸡组织(肝脏、肾脏、肌肉、脂肪)经酸化乙腈提取,正己烷除脂净化,以0.1%甲酸水溶液和乙腈为流动相进行梯度洗脱,同位素内标法定量。结果显示:恩诺沙星、环丙沙星均在线性范围内呈现良好线性关系,相关系数(R2)均大于0.99;恩诺沙星、环丙沙星在组织中的检测限为1μg/kg,定量限为5μg/kg;两种药物的平均回收率范围为75.5%～106%,批内、批间变异系数均小于11.2%。研究表明,该方法样品前处理简单,灵敏度高,适用同时检测鸡组织(肝脏、肾脏、肌肉、脂肪)和鸡蛋中恩诺沙星、环丙沙星残留。 相似文献
5.
盐酸沙拉沙星在肉鸡组织中的残留 总被引：7，自引：1，他引：6
建立了肉鸡组织中盐酸沙拉少星的HPLC测定方法，并测定了肉鸡单次内服（每1kg体重10mg)和连续5d混饮（每1L饮水50mg）给药后组织中的沙拉沙星残留特征。组织样品用乙腈-氨水匀浆，离心后取上清液用正已烷-乙醚除脂，水相用磷酸酸化后作HPLC检测。色谱柱为Hypersil C18柱，此外检测波长280nm。流动相为乙腈-2%西丁基溴化铵（体积比10：90），用磷酸调pH值至2.5。肝脏、肾脏、肌肉中沙拉沙星的检测限为0.05μg/g，组织样品回收率均大于90%。鸡单次内服盐酸沙拉沙星后，24h内各组织中均可检出药物，48h后肝脏、肾脏、肌肉中药物残留量均低于0.05μg/g；混饮停药后，6h内肝脏、肾脏中可检出残留药物，12h后3种组织中残留量均低于0.05μg/g。结果表明，盐酸沙拉沙星的肉鸡组织中消除迅速，连续治疗剂量用药后组织中无药物蓄积。 相似文献
6.
长效盐酸多西环素注射液在猪体内残留的消除规律 总被引：1，自引：0，他引：1
在常规饲养条件下,对35头健康成年猪按10 mg/kg体质量的剂量肌肉注射10%长效盐酸多西环素注射液,给药2次,给药间隔时间为48 h。第2次给药后12 h及2、5、9、14、192、5 d分别屠宰5头猪,分别采取每头猪的肌肉、肝脏、肾脏、皮肤+脂肪和注射位点肌肉等5种组织,用高效液相色谱法进行残留量测定。结果表明:在第2次给药后19 d,多西环素在各组织均能检测到,且残留均低于残留限量。多西环素残留浓度大小顺序:注射部位(肾脏(肝脏(皮脂(肌肉。采用WT1.4软件制定的统计方法来处理猪组织中药物浓度-时间数据,以制定休药期。 相似文献
7.
建立高效液相色谱荧光检测法测定牛羊组织中碘醚柳胺残留量。牛羊组织中残留的碘醚柳胺,经乙腈-丙酮溶液提取,阴离子交换固相萃取柱净化,高效液相色谱-荧光法测定,外标法定量。碘醚柳胺在10～5000 ng/mL的浓度范围内呈现良好的线性关系,碘醚柳胺在牛羊肌肉、肝脏、肾脏、脂肪中的检测限均为2.5 μg/kg,定量限均为5 μg/kg,牛羊肌肉、肝脏、肾脏、脂肪中15～500 ng/g添加浓度范围内的回收率为77.4%～93.8%,批内批间RSD值均<15％。 相似文献
8.
采用平衡透析法结合反相高效液相色谱法（RP—HPLC）测定鸡血浆中喹胺醇的蛋白结合率。色谱条件为ZORBAX Eclipse XDB C18色谱柱（250mm×4．6mm,5μm）,流动相为乙腈：水（40：60,v／v）,流速1．0mL／min,检测波长352nm。结果显示,喹胺醇浓度为1．1、5．5、22．0μg／mL缓冲液中鸡血浆的蛋白结合率分别为84．41％、75．30％、66．96％;透析平衡时间约为30h。表明喹胺醇的鸡血浆蛋白结合率较高,药物浓度与血浆蛋白结合率之间呈反比关系。 相似文献
9.
建立了高效液相色谱串联质谱（HPLC—MS／MS）法用于乙酰甲喹及其6种主要代谢物的检测。将65只健康白羽鸡分为两组,10只为空白对照组,其余为试验组。试验组施以20mg／kgb．w．灌胃乙酰甲喹悬浊液,1d2次,连续3d。在给药结束后2、4、6、12、16、24、30、36、48、72、120h分别宰杀5只鸡,采集血液和可食性组织（肌肉、肝脏、肾脏和皮脂）样品。结果表明：乙酰甲喹原药在组织和血浆中迅速消除;乙酰甲喹的代谢物广泛存在于鸡的可食性组织和血浆中;代谢物的残留消除过程较为复杂,代谢物在肌肉和肝脏中残留量较多,在皮脂中残留时间最长。研究结果将有助干榍示7．酷甲喹存鸡体内的砖留消除期,律． 相似文献
10.
为了测定牛肌肉、肝脏、肾脏组织中双氯芬酸钠的残留量,研究建立了一种用于该检测的高效液相色谱-串联质谱(LC-MS/MS)方法。试验将样品中加入乙腈提取液重复提取2次,超声波辅助提取,提取液中加入C18、PSA混合吸附剂进行净化。色谱柱采用C18反相柱对药物进行分离,洗脱液采用乙腈-0.1%甲酸水进行等度洗脱,配备电喷雾离子源,药物在正离子模式下电离,多反应监测模式(MRM)分段扫描测定检测结果,并采用内标法定量分析。结果表明,双氯芬酸在0.5～100μg/L浓度范围内有良好的线性关系,相关系数大于0.99;在牛的肌肉、肝脏和肾脏组织中检测限(LOD,S/N=3)为0.5μg/kg,定量限(LOQ,S/N=10)为1.0μg/kg。在3个浓度添加水平下,平均回收率为97.7%～114%,日内变异系数0.5%～4.7%,日间变异系数为0.9%～7.5%。该方法操作简便、结果准确,满足兽药残留检测方法规定要求,运用于快速准确测定牛肌肉、肝脏、肾脏等组织中双氯芬酸钠的残留。 相似文献
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Ovine scrapie was first recorded in Cyprus in 1985. Subsequently four dairy goats kept in two mixed flocks with affected sheep developed characteristic clinical signs similar to those seen in sheep. Fifteen goats from the two flocks were examined histologically and neurological lesions consistent with a diagnosis of scrapie were found in the four animals and in three others which had subsequently developed early neurological signs. These lesions were similar to those of naturally-affected sheep although neuronal degeneration and vacuolation were more severe in some cases. 相似文献
13.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
18.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献19.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
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In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
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