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1.
Tension and intracellular free calcium concentration [( Ca2+]i) were measured simultaneously in single smooth muscle cells isolated from the anterior byssus retractor muscle (ABRM) of Mytilus edulis that were loaded with the fluorescent Ca2+ indicator fura-2. Electrical stimulation evoked a transient elevation of [Ca2+]i associated with a "catch" contraction. During the catch state, however, [Ca2+]i was effectively at its resting level and was unaffected by 5-hydroxytryptamine, which induced a rapid relaxation from catch. The results indicate that a maintained high [Ca2+]i is not required for the maintenance of catch tension in intact ABRM and that there was no significant change in [Ca2+]i upon abolition of catch. 相似文献
2.
Calcium ion release in mechanically disrupted heart cells 总被引：5，自引：0，他引：5
In cardiac muscle fibers which have had their sarcolemma disrupted intracellular stores of calcium ions can be released by the same chemical stimuli which cause their release from the sarcoplasmic reticulum of skinned skeletal muscle fibers. These stimuli are increases in calcium or caffeine concentrations and substitution of chloride for propionate or sodium for potassium in solutions bathing the fibers. 相似文献
3.
By use of phenazine methosulfate and the "ncubation mixture film method," lactate dehydrogenase activity has been demonstrated in the dystrophic muscle fibers of strain 129 mice. The results indicate that for demonstration of lactate dehydrogenase activity in dystrophic muscle fibers phenazine methosulfate is necessary. This finding is typical for the "white" muscle fibers in the normal muscle and suggests that the dystrophy affects primarily the "white" muscle fibers. 相似文献
4.
Regenerative calcium release within muscle cells 总被引：29，自引：0，他引：29
Free calcium appears to trigger the release of stored calcium from the sarcoplasmic reticulum of skinned skeletal muscle fibers immersed in solutions with a low concentration of magnesium ion. 相似文献
5.
Calcium-sensitive cross-bridge transitions in mammalian fast and slow skeletal muscle fibers 总被引：8，自引：0，他引：8
The fundamental mechanism underlying the differing rates of tension development in fast and slow mammalian skeletal muscle is still unknown. Now, in skinned (membrane-permeabilized) single fibers it has been shown that the rate of formation of the strongly bound, force-producing cross-bridge between actin and myosin is calcium-sensitive in both fast and slow fibers and that the rate is markedly greater in fast fibers. The transition rates obtained at high calcium concentrations correlated with myosin isoform content, whereas at low calcium concentrations the thin filament regulatory proteins appeared to modulate the rate of tension development, especially in fast fibers. Fiber type-dependent differences in rates of cross-bridge transitions may account for the characteristic rates of tension development in mammalian fast and slow skeletal muscles. 相似文献
6.
Histochemical phosphorylase activity in regenerating muscle fibers from myophosphorylase-deficient patients 总被引：2，自引：0，他引：2
Fresh frozen sections of mature skeletal muscle fibers from patients with genetically determined "absence" of skeletal muscle phosphorylase (McArdle's disease) have no histochemical phosphorylase activity. That regenerating muscle fibers, in vitro and in vivo, from such patients do have histochemical phosphorylase activity present suggests a loss of enzyme activity with fiber maturity. 相似文献
7.
Mammalian motor units: physiological-histochemical correlation in three types in cat gastrocnemius 总被引：20，自引：0，他引：20
The correlation among a variety of physiological properties and the histochemical characteristics of muscle fibers belonging to single motor units in a mixed mammalian muscle is directly demonstrated. The population of motor units making up the cat gastrocnemius was classified into three nonoverlapping groups on the basis of a combination of physiological parameters. The muscle fibers belonging to motor units of each physiological type exhibited a distinctive histochemical profile, such that the three basic histochemical "fiber types" exactly matched the three physiologically defined groups. Within each individual motor unit, the muscle fibers were histochemically uniform. 相似文献
8.
Calcium currents in skeletal muscle fibers of an insect, Carausius morosus, inactivate under depolarization. This inactivation depends on the current being carried across the membrane by calcium ions, rather than strontium or bariumions. 相似文献
9.
Membrane of crayfish muscle fibers becomes selectively permeable to sodium when the calcium concentration of the bathing medium is reduced. Removal of calcium or its reduction below 1 or 2 millimole per liter causes large transient depolarizations up to 70 millivolts in amplitude. They resemble pro longed action potentials and occur only in the presence of sodium. The responses are abolished when tris(hydroxymethyl)aminomethane or lithium is substituted for sodium, and are blocked by tetrodotoxin even in the presence of sodium. 相似文献
10.
Deuterium oxide: inhibition of calcium release in muscle 总被引：1，自引：0，他引：1
Calcium release, measured as luminescence of the protein aequorin, was measured simultaneously with membrane potential and isometric tension in single muscle fibers of the barnacle (Balanus nubilus). Deuterium oxide inhibited calcium release and isometric tension but did not affect membrane potential, a result consistent with the postulate that deuterium oxide inhibits the coupling between excitation and contraction. 相似文献
11.
用琥珀酸脱氢酶染色法对不同生长阶段(4,8,12,16周龄)固始鸡胸肌和腿肌的肌纤维类型进行了分析,并对肌红纤维与中间型肌纤维面积之和(SR+M)、中间型肌纤维面积(SM)和白肌纤维面积(SW)进行了测定。结果表明,腿肌肌纤维类型有3种,即红肌纤维,白肌纤维和中间型肌纤维,而胸肌肌纤维类型有2种,即白肌纤维和中间型肌纤维。在固始鸡的生长过程中,腿肌的白肌纤维比例逐渐降低,胸肌的白肌纤维的比例先升后降。公鸡腿肌中SR+M／SW值在第4,8与12周龄间差异均不显著(P>0．05),在第12周龄与16周龄间差异显著(P< 0．05),在第8周龄与第16周龄间差异极显著(P<0. 01);母鸡腿肌中SR+M／SW值在各周龄间差异均不显著 (P>0．05);公鸡胸肌中SM／SW值在各周龄间差异均不显著(P>0．05);母鸡胸肌中SM／SW除在第8周龄和第12 周龄间差异不显著(P>0．05)外,在其他各周龄间差异均极显著(P<0．01)。腿肌中的红肌纤维和中间型肌纤维以及胸肌中的中间型肌纤维在公鸡肌肉中的含量均高于母鸡。 相似文献
12.
【目的】研究猪肌肉肌纤维特性与肌肉品质的关系及品种、性别差异。【方法】国内外相关文献的收集、汇总及分析。【结果】①肌纤维的形成是一个非常复杂的生物学过程,肌纤维数目、大小及类型是肌纤维的基本特性。②肌纤维类型的划分,目前最常用的是根据肌纤维中MyHC基因的多态性表达对肌纤维进行分类。③出生前,MyHC异构体按照胚胎期→胎儿期→成熟期顺序表达,出生后,肌纤维类型具有相互转化的特性。④不同类型肌纤维由于代谢特征存在明显差异,因此会影响肌肉色泽、pH、系水力等肌肉品质性状。肌纤维大小、密度与肌纤维类型密切相关,因此也会影响肌肉品质。⑤不同品种猪肌纤维特性有明显差异,因而表现出不同的肌肉品质。为了适应各自生长发育及生理需要,猪不同性别间肌纤维特性也有所不同。【结论】猪肌肉肌纤维特性直接影响肌肉品质的优劣,品种、性别不同,肌纤维特性的形成有一定差异,表现出不同的肌肉品质。 相似文献
13.
In 29 acutely psychotic patients (mostly schizophrenic), histochemical abnormalities of a myopathic type were demonstrated in skeletal muscle biopsies from 13 and were generally correlated with elevation of the "muscle" type isoenzymes of creatine phosphokinase in the patients' serum. The incidence was much higher than found in normal controls, hospitalized neurotic psychiatric patients, or parents of acutely psychotic patients. A diazo-coupling type of "alka-line phosphatase" reaction was particularly useful in identifying abnormal muscle fibers. 相似文献
14.
宁夏几个主要品种猪背最长肌组织学特性的研究 总被引：2，自引：0，他引：2
从肌肉的组织结构出发,用组织学、化学与肉质测定方法,系统测定了宁夏5个主要猪品种的背最长肌肌内的脂肪含量、羟脯氨酸含量、肌肉的剪切力、肌纤维直径、肌束内肌纤维根数、肌纤维密度、肌纤维间距和肌大束间距.结果表明,梅山猪、宁夏黑猪和杜洛克猪肌内脂肪含量高于长白猪和约克夏猪(P<0.05).梅山猪和宁夏黑猪肌肉的剪切力、羟脯氨酸含量、肌纤维直径和肌纤维间距均低于杜洛克猪、长白猪和约克夏猪(P<0.05),肌束内肌纤维根数(P>0.05)、肌纤维密度和肌大束间距高于上述3个国外品种(P<0.05). 相似文献
15.
本研究旨在揭示斑马鱼下颌再生中神经和肌肉的关系。通过HE染色技术、免疫荧光组织化学等实验技术分别观察斑马鱼下颌神经和肌肉的再生过程,考察神经和肌肉的关系。研究结果显示,截面附近的神经纤维在剪切后5 h降解,剪切后2 d时完全消失。剪切后3 d,整个芽基所在位置已出现新生的神经纤维。剪切5 d时,芽基上布满大量的神经纤维。肌纤维在剪切后12 h已发生断裂,剪切后5 d形成芽基细胞并分化成各种前体细胞如肌肉前体细胞,剪切后9 d出现新生的肌细胞,剪切后14 d新生的肌细胞与新生的神经纤维建立神经肌肉接头联系,30 d后成为成熟的肌肉组织。结果表明:如果斑马鱼1/3的下颌被人为切除,下颌肌肉可以实现功能性再生,同时神经极可能在肌肉再生中发挥作用。但是如果切除部分超过1/3,则下颌无法再生复原,其原因有待进一步研究。 相似文献
16.
Frog skeletal muscle fibers: changes in electrical properties after disruption of transverse tubular system 总被引：12，自引：0，他引：12
In muscle fibers which have been exposed for 1 hour to a Ringer solution containing 400 millimolar glycerol and then returned to plain Ringer solution, the transverse tubular system is disrupted. At the same time the membrane capacitance is markedly reduced and hyperpolarizing current pulses no longer produce a slow, progressive increase in potential (creep). The large capacitance of muscle and the phenomenon of "creep" must both depend on an intact transverse tubular system. 相似文献
17.
为研究狮头鹅和乌鬃鹅在不同生长阶段肌纤维的发育规律,分别选取健康的１、４０和１２０日龄乌鬃鹅和狮头鹅,屠宰并取其胸肌和腿肌做石蜡切片,观察并测定两种鹅不同部位不同时期肌纤维的直径和横截面积．结果表明两种鹅１日龄胸肌肌纤维差异不明显,腿肌肌纤维横截面积和直径差异显著（Ｐ＜０.０５）;４０日龄两种鹅胸肌肌纤维横截面积差异显著（Ｐ＜０.０５）,但胸肌直径和腿肌的直径和横截面积差异不显著（Ｐ＞０.０５）;１２０日龄两种鹅胸肌肌纤维和腿肌肌纤维的横截面积和直径差异极显著（Ｐ＜０.０１）．研究结果表明乌鬃鹅肌肉纤维在１至４０日龄期间生长发育迅速,但４０日龄后肌纤维生长潜力不如狮头鹅． 相似文献
18.
渗钙对‘伏’苹果衰老,过氧化物酶和IAA氧化酶活性的影响 总被引：4，自引：0，他引：4
渗钙处理明显保持“伏”苹果的硬度,降低衰败指数和呼吸速率。对照（渗水）果实的阴离子过氧化物酶（ＰＯＤ）和阳离子过氧化物酶活性稳步下降,钙处理后最初５ｄ时,ＰＯＤ活性显著增强。随果实衰老,处理与对照的酶活性逐渐接近,末期（采用２５ｄ）时上述酶活性的差别消失,同时,钙处理可提高果实的ＩＡＡ氧化酶活性,这种效应可持续２０ｄ。此外发现,钙处理果实阴离子ＰＯＤ和阳离子ＰＯＤ同工酶中皆比对照多１条酶带（Ｒｆ０ 相似文献
19.
Sarcomeric oscillations in frog skeletal muscle fibers 总被引：1，自引：0，他引：1
Brief asynchronous, small-amplitude, cyclic, longitudinal displacements of the striations of frog skeletal muscle fibers were observed with ordinary light microscopy after application of caffeine and certain quaternary ammonium compounds. With time these oscillations became synchronized and evolved into peristaltic-like movements. The oscillations were influenced by sarcomere length, temperature, external concentration of calcium ions, membrane potential, and disruption of the transverse tubules. 相似文献
20.
Phosphate release and force generation in skeletal muscle fibers 总被引：28，自引：0，他引：28
Rapid laser pulse-induced photolysis of an adenosine triphosphate precursor in muscle fibers abruptly initiated cycling of the cross-bridges. The accompanying changes in tension and stiffness were related to elementary mechanochemical events of the energy-transducing mechanism. When inorganic phosphate was present at millimolar concentrations during liberation of adenosine triphosphate in the absence of calcium, relaxation was accelerated. Steady active tension in the presence of calcium was decreased but the approach to final tension was more rapid. These results suggest that, during energy transduction, formation of the dominant force-generating cross-bridge state is coupled to release of inorganic phosphate in a reaction that is readily reversible. 相似文献