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1.
利用TP-M13-SSR标记构建苹果栽培品种的分子身份证 总被引：2，自引：0，他引：2
以国家果树种质兴城梨、苹果圃保存的314份来自19个国家的苹果栽培品种为试材,利用TP-M13-SSR标记,基于TP-M13-SSR指纹图谱构建苹果种质分子身份证。16对SSR引物在供试种质间共检测出等位基因357个,平均每对引物检测到22.3个。根据引物扩增等位基因数和Shannon’s指数,从1对引物开始逐步增加引物数量筛选可将供试材料全部区分的引物组合,最终确定6对核心引物可以区分全部供试苹果种质。基于供试苹果种质在6个SSR位点的指纹图谱,将等位基因编码成字符串获得分子身份证,利用条码技术其进一步转化成可被机器快速扫描的条码分子身份证,使每份种质具有可辨的分子身份证,达到利用最少、最特异引物区分最多苹果种质的目的。 相似文献
2.
《北方园艺》2020,(4)
以72个荷花品种为试材,采用荧光标记SSR结合毛细管电泳的方法,研究了不同荷花品种的遗传多样性,并构建了指纹图谱,以期为荷花种质资源的收集和分类鉴定提供依据。结果表明:利用9对SSR荧光引物,通过毛细管电泳技术对72个荷花品种进行检测,共检测到51个多态位点,多态位点数平均为5.67;多态性信息含量PIC值变化范围为0.34～0.76,平均值为0.56。利用其中6对引物CL04、SSR449、SSR412、CL01、CL16和CL10可区分72份供试品种。利用引物扩增带型组合法,构建了72个荷花品种的指纹图谱并进行聚类分析,在遗传相似系数0.535处供试荷花材料可分为三大类群。该研究结果可为荷花种质资源鉴定和分子育种提供参考依据。 相似文献
3.
利用开发的11对EST-SSR引物分析了33份猕猴桃种质资源的遗传多样性及其遗传关系。结果表明,11对EST-SSR引物在所有供试材料中均可扩增出清晰条带,其中有8对引物呈现多态性,多态性扩增率为72.7%,对33份种质材料的区分率达100%。8对多态性引物共检测到61个等位基因,每对引物可检测到的等位基因数为2-17,平均为7.6个。利用NTSYS-pc软件,以不加权成对算术平均法(UPGMA)对扩增结果进行聚类,谱系图显示,33份种质材料之间的相似系数在0.60～0.97,表明猕猴桃种质之间遗传关系相对来说不是很远。在相似系数0.73的水平上可将供试猕猴桃材料分为7个类群,其结果与传统的形态分类大体一致。从分子角度揭示了猕猴桃种质资源的遗传多态性及其遗传关系。可为猕猴桃种质改良提供理论依据。EST-SSR是非常有效和可靠的分子标记,可为猕猴桃分子育种及遗传连锁图构建奠定基础。 相似文献
4.
部分香蕉品种SSR指纹图谱的构建 总被引：6，自引：1，他引：5
采用SSR标记技术构建香蕉品种的分子指纹图谱,为香蕉品种鉴定提供依据。以56份香蕉种质为材料,从199对引物中筛选出5对多态性及稳定性较高的SSR引物进行PCR扩增。5对引物在56个品种中扩增出的多态性带数在11～16个,平均为13.8条;引物的多态信息含量(PIC)在0.8490～0.9022,平均0.8727。依据香蕉SSR带型特征,对每个引物生成的不同带型直接编号,简化香蕉SSR带型记录方法,并利用每个品种的带型编号,建立其DNA分子指纹图谱,5对引物可将56份供试香蕉材料完全区分开。表明SSR标记技术可用以鉴定香蕉种质。 相似文献
5.
利用SSR标记技术对12个黄桃品种（系）进行遗传多样性检测.结果发现,SSR标记能够揭示材料间的遗传多样性,从蔷薇科苹果属35对SSR引物中筛选出8对扩增稳定的特异性引物,构建其指纹图谱.8对SSR引物共扩增出78条DNA片段,其中44条具有多态性,多态性比率为54.6％,材料间的遗传相似系数在0.64 ～0.88之间,平均为0.759.通过聚类,从分子水平对黄桃品种（系）的遗传关系进行分析,并对12份黄桃种质材料进行了SSR标记分类,为黄桃种质资源的研究利用提供参考. 相似文献
6.
野生毛花猕猴桃果实表型性状及SSR遗传多样性分析 总被引：2，自引：0，他引：2
以江西省武功山境内的70份野生毛花猕猴桃种质资源为试材,对其果实表型性状、SSR遗传多样性及其亲缘关系进行分析和评价。根据UPOV（国际植物新品种保护联盟）公布的猕猴桃属品种测定标准对供试材料的果实表型性状进行观测。参照猕猴桃遗传连锁图谱,选用分布于中华猕猴桃基因组中的70对SSR引物对供试材料进行多态性分析。结果表明,在70对引物中,21对引物成功扩增出多态性片段。随机采集的70份野生毛花猕猴桃种质资源中,其果实表型性状和DNA分子水平均存在丰富的遗传多样性。基于UPGMA聚类分析将供试的野生毛花猕猴桃资源分为果实圆形和椭圆形混合组、果实椭圆形组以及果实圆柱形组。21对多态性高的SSR引物共检测出127个等位变异位点,变异范围在2 ~ 12之间,平均每对SSR引物可检测到6.04个等位位点。遗传相似系数（GS）变异范围为0.5306 ~ 0.9252。GS值在0.65水平上UPGMA聚类分析可将供试的野生毛花猕猴桃种质资源划分成Ⅰ、Ⅱ和 Ⅲ 共3个组,这与果实表型性状分析的结果基本一致。这些遗传变异丰富的种质资源可为猕猴桃育种提供有价值的材料。 相似文献
7.
【目的】应用荧光SSR分子标记构建柚类种质分子身份证，用于品种资源鉴定。【方法】收集22份柚类材料，筛选SSR荧光标记引物，采用荧光毛细管电泳检测技术分析扩增条带分子质量和等位基因，获得相应的扩增带型，从而对每份柚材料进行标记。采用个位阿拉伯数字和小写英文字母对不同等位基因分子质量大小进行编码，再按照引物扩增带型数由大到小顺序将各位点赋值编码的数字串联排序，构建供试样品的分子身份证。【结果】筛选出4对多态性较好的引物，使用这4对引物共检测到49个扩增带型和41个SSR等位位点，平均每对引物的有关带型数12.25个、位点数10.25个。【结论】通过扩增带型分析，构建了22份柚类种质的分子身份证，包括12条信息独特的身份证。遗传聚类与分子身份证分析结果一致，为柚类品种鉴定和保护提供了重要依据。 相似文献
8.
利用核基因组简单序列重复（Simple sequence repeat,SSR）DNA标记,对11份化州橘红及其相关种质的遗传多样性进行了研究。结果表明：6对SSR分子标记引物共扩增出32个等位基因,每对引物可扩增3～8个等位基因;11份种质在SSR位点上的PIC值介于0.1763到0.4400之间,平均为0.2761。聚类分析表明,在Nei s遗传相似系数0.85处,可将所研究的种质分为6组,其中有5组分布有化州橘红种质,表明化州橘红不同种质间有较大的遗传差异。 相似文献
9.
以33份暗褐网柄牛肝菌样品为试材,采用SSR标记技术,对其遗传多样性进行研究,以期为暗褐网柄牛肝菌商业菌株鉴定奠定基础。结果表明:15个SSR标记中有5个标记在供试样品中表现出多态性,每个标记检测到2~6个等位基因,平均3.20个,Shannon′s信息指数平均值0.76,多态性信息含量平均值0.43。聚类分析结果显示,在相似系数为0.99时,供试材料可被分为11个类群,对应11种多位点分子表型,与样品的来源有较好的对应关系。根据多位点分子表型构建了供试材料的SSR指纹图谱。 相似文献
10.
在全基因组水平利用分子标记技术,构建甜瓜种质核酸指纹库,建立早期、快速、准确的甜瓜种子纯度、真实性及资源的遗传血缘检测技术.
1 关键技术核心
筛选出用于构建甜瓜核酸指纹库的核心引物是关键技术问题.利用20份具有代表性的甜瓜品种(系)筛选1219对SSR引物,得到多态性的引物470对.综合考虑扩增条带统计难易、多态性信息含量(PIC)、整合遗传连锁图谱(ICUGI,2011,http:∥www.icugi.org/.)等多种因素,最终选择其中18对扩增多态性丰富的引物作为核心引物.经过18对引物扩增后,可以对供试甜瓜品种(系)中的每一份材料建立了一个能区别于其他供试材料的指纹图谱代码. 相似文献
11.
Anna Lisek Danuta Kucharska Agnieszka Głowacka Elżbieta Rozpara 《The Journal of Horticultural Science and Biotechnology》2017,92(5):484-492
The sour cherry is known to exhibit the phenomenon of gametophytic self-incompatibility which prevents self-fertilization. In sour cherry, besides self-incompatible cultivars, there often occur self-compatible cultivars. This is due to the occurrence of natural mutations of the S-RNase or SFB genes and, consequently, loss of functionality of S-alleles. Here we present the results of the identification of S-haplotypes of 21 cultivars of sour cherry from various regions of Europe. The analyses were performed using methods based on the amplification of intron I and intron II of the S-RNase gene and fragments specific to the individual alleles of the S-RNase or SFB genes. The tested cultivars were found to contain 15 S-haplotypes: S1, S1?, S4, S6, S6m, S6m2, S9, S12, S13, S13?, S26, S35, S36a, S36b, and S36b2. The most frequently occurring S-haplotypes were S13? (61.9%), S36a (57.1%), and S26 (47.6%). On the basis of the results, 17 of the 21 cultivars were deduced to be self-compatible. The results will be of use in the production of sour cherry fruit by facilitating the selection of suitable pollinating cultivars. The results are also expected to be useful in the breeding of new cultivars of sour cherry when selecting genotypes for crosses. 相似文献
12.
Volatile organic compounds (VOC) are emitted by many plants. In this study, sixty common plant species of the Vidarbha region of Maharashtra, India were examined for VOC (isoprene and monoterpene) emissions. Plant species VOC emission rates ranged from undetectable to 75.2 μg g?1 h?1. Dalbergia sissoo exhibited a maximum VOC emission rate of 75.2 μg g?1 h?1. Ozone forming potentials (OFP) of the sixty plant species were also estimated using the method of Benjamin and Winer (1998). Maximum ozone forming potential of 77 g O3 (tree)?1 d?1 was observed in the case of Mangifera indica. Out of 60 species, 26 species (43.3%) had low OFP (less than 1 g O3 (tree)?1 d?1), 18 species (30%) had medium OFP (less than 1–10 g O3 (tree)?1 d?1) and 16 species (26.7%) had high OFP (more than 10 g O3 (tree)?1 d?1). 相似文献
13.
金嘴蝎尾蕉切花苞片褐变的控制 总被引：3，自引：0，他引：3
研究了金嘴蝎尾蕉切花最佳采收时期和对其苞片褐变具有良好控制效果的预处理保鲜液及瓶插方法。结果表明: 金嘴蝎尾蕉以中蕾期采收最佳; 整个花枝水平放置, 完全浸泡于500 mg·L - 1 H3BO4或500 mg·L - 1 MgSO4中1 h后, 结合2 mg·L - 1 6-BA + 200 mg·L - 1 Al2 ( SO4 ) 3瓶插保鲜及0.1%聚乙烯醇喷洒花枝, 可显著控制苞片褐变, 保持组织较低水平pH值, 减缓膜透性的上升。其瓶插寿命分别达12 d和11.5 d, 显著高于对照的4 d。 相似文献
14.
京白梨等品种S 基因型鉴定及新基因S28 和S30 的核苷酸序列分析 总被引：8，自引：3，他引：8
根据梨S基因高度保守区C1和C3区, 设计1对引物P1和P2, 对梨品种的基因组DNA进行S基因特异扩增、克隆、测序, 并在GenBank中BLAST比较, 确定S 基因特异性片段, 对京白梨等6个供试自交不亲和品种的S基因型比对结果为: 白梨中的‘库尔勒香梨’为S21 S28 , ‘苹果梨’为S17S19 ; 砂梨中的‘台湾蜜梨’为S11 S22 ; 西洋梨中的‘葫芦梨’为Sa Sb; 秋子梨中的‘京白’为S16 S30 , ‘早梨18’为S4 S28。其中S28和S30为首次登录的新S 基因, 在GenBank的登录号分别为AY562394 (库尔勒香梨) 和AY876945 (京白) 。 相似文献
15.
Comparison of anthocyanins in non-blotches and blotches of the petals of Xibei tree peony 总被引：1，自引：0，他引：1
Jingjing Zhang Liangsheng Wang Qingyan Shu Zheng’an Liu Chonghui Li Jie Zhang Xiaolei Wei Daike Tian 《Scientia Horticulturae》2007
A comparison in non-blotches and blotches of 35 cultivars of Xibei tree peony was investigated to explore flower coloration of Xibei tree peony (the second cultivar group in Chinese tree peony). With high performance liquid chromatography (HPLC) analysis, six anthocyanins including peonidin 3,5-di-O-glucoside (Pn3G5G), peonidin 3-O-glucoside (Pn3G), cyanidin 3,5-di-O-glucoside (Cy3G5G), cyanidin 3-O-glucoside (Cy3G), pelargonidin 3,5-di-O-glucoside (Pg3G5G), and pelargonidin 3-O-glucoside (Pg3G) were detected in non-blotches and blotches of petals. Total anthocyanins (TA), total flavones and flavonols (TF), and the copigmentation index (CI) were also analyzed. Cyanidin-based glycosides, which accumulated abundantly at the basal petal, resulted in blotch formation. Some peculiar cultivars with only one kind of anthocyanins or without anthocyanins in non-blotches were found in this study. For example, ‘Feng Zi Xiu Se’, ‘Ou Duan Si Lian’, and ‘Xi Wang’ contained only Pn3G5G; ‘Bing Shan Cang Yu’ and ‘Jin Bo Dang Yang’ contained only Cy3G; while no anthocyanins were found in ‘Bing Shan Xue Lian’. Several cultivars were rich in Pg-based glycosides, such as ‘Ni Hong Huan Cai’, and ‘Ju Yuan Shao Nv’. These cultivars would be excellent parents for creating new cultivars with novel flower color in the future. 相似文献
16.
《Scientia Horticulturae》2005,104(2):169-178
The Japanese pear ‘Osa-Nijisseiki’ (S2S4SM) (SM = stylar-part mutant) is a self-compatible bud mutant that originated from self-incompatible ‘Nijisseiki’ (S2S4). The S4-allele of the pear is deleted and it shows unilateral incompatibility to cultivar with an S2S4 genotype. However, when pollen-tube growth was compared between cross-compatible [‘Osa-Nijisseiki’ × ‘Okusankichi’ (S5S7)], unilateral-compatible [‘Osa-Nijisseiki’ × ‘Kikusui’ (S2S4)], self-compatible (‘Osa-Nijisseiki’ × ‘Osa-Nijisseiki’), and unilateral-incompatible pollination (‘Kikusui’ × ‘Osa-Nijisseiki’), pollen-tube growth clearly showed the following order: cross-compatible > unilateral-compatible > self-compatible > unilateral-incompatible. This indicates that the ‘Osa-Nijisseiki’ style produces specific inhibitor(s) not only to S2- and S4-pollen but also “self-pollen”, because the phenotype of S4SM-pollen is the same as S4-pollen. Stylar protein analysis demonstrated that ‘Osa-Nijisseiki’ produces S2-RNase (RNase associated with S2-allele) together with a small amount of S4-RNase. The purified S4-RNase possessed almost the same inhibitory action on the growth of S4-pollen-tubes in vitro at 1 μg μl−1 as that from original ‘Nijisseiki’. These results suggest that the depressed growth of unilateral-compatible and self-pollen-tubes in ‘Osa-Nijisseiki’ is due to this biologically active S4-RNase. Growth of self-pollen-tubes may also be depressed by inhibitor(s) specific to “self-pollen” unrelated to S-alleles. 相似文献
17.
化学药剂对香椿休眠解除的影响及其机理 总被引：3，自引：0，他引：3
采用五元二次正交旋转组合设计, 探索6-BA、GA3 、(NH2) 2CS、ZnSO4 、KNO3 对香椿休眠解除的影响, 5 种化学药剂解除休眠的作用依次为ZnSO4 > (NH2) 2CS > GA3 > 6-BA > KNO3 。打破香椿休眠的最佳药剂组合是: 6-BA 20 mg/L + GA3 180 mg/L + (NH2) 2CS 1 % + ZnSO4 0.5 % + KNO3 2 %。用上述药剂处理可以使香椿提早23 d 萌芽。 相似文献
18.
《Scientia Horticulturae》2001,89(1):9-21
This paper analyses the response of vegetative growth of greenhouse tomato to both root-zone salinity and shoot-environment (potential transpiration), with the purpose of explaining the observed lack of effect on dry matter yield. A reference salinity (EC) of 2 dS m−1 was compared in three experiments with, respectively, 6.5, 8 and 9.5 dS m−1. Another experiment investigated specific effects of sodium chloride, by comparing two high-EC treatments (both 9 dS m−1), one with a high concentration of nutrients and one with addition of sodium chloride to a normal nutrient solution. The shoot-environment was either a “normal” climate regime or the same regime but with depressed potential transpiration, mainly by adaptation of the humidity set point. There was no detectable effect of the potential transpiration treatment, neither of the sodium chloride. Salinity effects on vegetative growth only showed up at EC exceeding 6.5 dS m−1. The most evident EC effect was a reduction of leaf expansion; individual leaf area was reduced by 8% per dS m−1 exceeding 6.5. This was partly compensated by a slight increase (2% per unit EC) in the number of leaves, which explains why cumulative plant leaf area decreased by about 7% per unit EC in excess of 6.5 dS m−1. Therefore, leaf area index (LAI) at the highest EC was reduced by some 20% compared to the LAI at an EC of 2 dS m−1. It is estimated that this would cause a reduction of less than 8% in light interception, and thus in dry matter produced. Indeed, differences observed in dry weight between the EC treatments were never significant. 相似文献
19.
Seven chilli landraces of Manipur belonging to three cultivated species of Capsicum (Capsicum annuum, Capsicum frutescens, and Capsicum chinense) form economically important food crops of the region. The genotypes were characterized using ten random amplified polymorphic DNA (RAPD) markers. The cluster analysis based on Jaccard's similarity coefficient calculated by UPGMA method differentiated the genotypes into two main cluster groups. One cluster represented the C. annuum genotypes while the other cluster represented the C. frutescens and the C. chinense genotypes. C. chinense genotypes were more close to C. frutescens genotypes. Genetic variation between the C. frutescens genotypes was more than among the C. annuum genotypes and the C. chinense genotypes were the least similar ones. 相似文献
20.
5种国兰( Cymbidium ) 的光合特性 总被引：12，自引：1，他引：12
通过气体交换和叶绿素荧光分析等方法研究了5种国兰(Cymbidium ) 的光合特性。其光饱和点、光补偿点、CO2 补偿点和CO2 饱和点表明它们具有C3 植物特征; 光合速率在3.0 ～5.9 μmol·m- 2 ·s- 1之间, 在低于2 000 μmol·mol- 1 CO2 浓度下, 光合速率受CO2 浓度影响较大; 5种国兰的光合能力、表观量子效率、羧化效率等都有所差异; 光饱和点在350～650μmol·m- 2 ·s- 1之间, 属于喜阴植物,其中春兰、建兰耐阴性相对较低, 墨兰、春剑耐阴性相对较高, 蕙兰居中。从暗处暴露到强光下, 5种国兰的光化学反应启动差异较大, 启动后的光化学效率差异也较大, 这可能与国兰不同种起源地的生态环境,适应性及进化有关。 相似文献