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1.
Agrobacteriurn tumefaciens-mediated transformation (AtMT) method was used to study the genetic transformation in Rhizopus arrhizus of antisense expression vector pBI121-fad2 of VeFAD2 gene. We studied the key factors influencing transformation, such as A.tumefaciens strains,bacterial cell volume initially used, co-cultivation time, effective period and concentration of AS. The results shows that the ideal A.tumefaciens strain is AGL-1, the optimum bacterial cell volume initially used is 50-100 μL and co-culture 24 hours will get the most transformants. At co-culture period, AS induction is indispensable. It is beneficial to improve transformation ratio by adding to 200 μmol·L-1 AS at preincubate period and increasing AS concentration to 400-600 μmol·L-1 at co-culture period. It determined that antisense VeFAD2 gene has been integrated into the genome of R. arrhizus by PCR-Southern detection.  相似文献   

2.
In this study, we investigated the optimal concentration of nutrient-mist and NaHSO3 for cutting propagation of Juniperus squamata 'Blue Star' , as well as the variation in the contents of chlorophyll in leaves, and solubility carbohydrate,soluble protein and other nutrition in leaves and bases of cuttings during rooting. Cuttings of grade Ⅲ (the lenghth of cutting bases lignified from 0.1 to 0.2 cm) was treated by 1/4MS+NaHSO3(200 mg·L-1),and the rooting rate rose 35% and root qualities were improved compared with control experiment, for there were different effects among three grade cuttings. The treatment of grade Ⅲ cuttings also resulted in higher content of soluble sugar and starch of those bases during rooting, especially obviously different for fifteen days, but less effect on the chlorophyll and some nutrition content of cutting leaves.  相似文献   

3.
An improved method for selection of large electro-fused protoplasts ofPopulus alba by a micromanipulator was developed. The conditions for electric cell fusion treatment were optimized. For the best result, protoplasts with a cell density of 5 × 105/mL were treated with an alternate current (1 MHz, 200 V/cm) and pulsed with a direct current (2 kV/cm) for 100μs in 2.5 mM CaCl2 and 0.55 M mannitol. The electo-fused protoplasts were cultured in NH4NO3-free Murashige and Skoog’s medium containing 0.6 M of mannitol, 0.09 M sucrose, 1μM of 2,4-dichlorophenoxyacetic acid and 0.1μM of benzyladenine, the same medium used for protoplast culture, but at a very low cell density of 5–10 × 102/mL in a well of a 96-well culture plate. When cell aggregates derived from individual fused protoplasts were transferred to fresh medium with 0, 0.3 or 0.6 M mannitol, large colonies developed. In the shoot differentiation medium, the reaction of the calluses derived from large fused protoplasts towards the growth regulators differed from the non-fused ones. In medium containing 1μM each of naphthalene acetic acid andN-(2-chloro-4-pyridyl)-N′-phenylurea, growth of callus from electro-fused ones was not reduced by much compared to the control, but shoot differentiation was inhibited. Gibberellic acid (0.1–10μM) was beneficial to shoot regeneration; however, irregularly shaped leaves appeared at high gibberellic acid concentrations. Shoots regenerated were rooted in Murashige and Skoog’s medium containing 4μM of indole-3-butyric acid. Some plantlets obtained had a varied morphology. Based on the characteristics of growth, some cells derived from electro-fused protoplasts appear to be physiologically different from the non-fused one.  相似文献   

4.
Photosynthetic responses to a series of 1-min lightflecks (1,000μmol m−2 s−1) superimposed on a background with different duration (1, 5, and 10 min) and intensity (25 and 50μmol m−2 s−1) of low background photosynthetic photon flux density (PPFD) were measured in the leaves ofFagus crenata grown in a gap and understory of aFagus crenata forest in the Naeba Mountains. The two background PPFD intensities most frequently occurred in understory and gap sites respectively. The maximum net photosynthetic rate (P Nmax) and maximum stomatal conductance (g smax) were higher in the gap seedlings than in the understory seedlings. However, when the background PPFD was 25μmol m−2s−1, the net photosynthetic rate (P 25) and stomatal conductance (g s25) were almost the same between the gap and understory. When the background PPFD duration was 1-min, the net photosynthetic rate (P N ) at the end of each lightfleck increased progressively. When the background PPFD duration was 5- and 10-min, the increase inP N at the end of each lightfleck was less. This indicates that background PPFD duration is important to photosynthetic responses to lightflecks. The higher ratios ofP 25/P Nmax andg s25/g smax in the understory seedlings indicate that the understory seedlings can maintain relatively lower levels of biochemical and stomatal limitations than the gap seedlings under low light conditions. The ratios ofP N /P Nmax at the end of each lightfleck (IS) and light utilization efficiency of single lightflecks (LUE s) that showed the influence of lightflecks on carbon gain were higher in the understory seedlings than in the gap seedlings when the background PPFD was 25μmol m−2 s−1. This means that understory seedling are capable of utilizing fluctuating light more efficiently under low light conditions than the gap seedlings although the net carbon gain of single lightflecks (CG s) in the understory seedlings was not higher than that in the gap seedlings. There were no significant differences inIS andLUE s between understory seedlings at a background PPFD of 25μmol m−2 s−1 and gap seedlings at a background PPFD of 50μmol m−2 s−1. However,CG s in gap seedlings was higher than in understory seedlings. These results provide more evidence thatF. crenata acclimate to a natural light environment in respect to relative induction state at low background PPFD and can capture the fluctuating light at the same efficiency in both the gap and understory seedlings under natural light environments. This study was funded by the research project, Evaluation of Total CO2 Budget in Forest Ecosystems, coordinated by the Ministry of Agriculture, Forestry and Fisheries of Japan.  相似文献   

5.
Glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) was purified from the leaves of 8-week-old Populus suaveolens cuttings. The enzyme activity in the absence and presence of reduced dithiothreitol (DTTred) was determined. The results show that the G6PDH activity is not inactivated by pre-incubation with DTTred, indicating that the purified enzyme probably presented in cytosol of P. suaveolens. The catalytic characteristics and kinetic parameters of cytosolic G6PDH purified from P. suaveolens cuttings were also studied. The results show that G6PDH is characterized by K m value of 360 μmol·L−1 for G6P and 16 μmol·L−1 for NADP, a pH range of 7.3-8.9, and the maximum activity around pH 8.2. The enzyme activity is inhibited by various metabolites such as NADPH, NADH, GTP, UTP, ATP, AMP, ADP, CoA, acetyl CoA, fructose-6-phosphate (F6P), erythrose-4-phosphate (E4P), ribose-5-phosphate (R5P) and 3-phosphoglycerate (3-PG) (all at 1 mmol·L−1 except for NADPH and NADH) to different extents. NADPH is the most effective inhibitor of enzyme activity, with an inhibition of 72.0%. The addition of metal ions such as MgCl2, CaCl2 and KCl (all 1.0 mmol·L−1) to the standard reaction mixture has no remarkable influence on the cytosolic G6PDH activity. However, CdCl2 (1.0 mmol·L−1) causes high inhibitory effect on the enzyme activity. To explore the role of G6PDH on the enhancement of freezing resistance induced by freezing acclimation, the changes in the cytosolic G6PDH activity and freezing resistance (expressed as LT50) of P. suaveolens cuttings during freezing acclimation at −20 °C were investigated. The results reveal that freezing acclimation decreases LT50 of cuttings, and increases the activity of cytosolic G6PDH compared with control ones, while 2 d of de-acclimation at 25 °C result in a decrease in cytosolic G6PDH activity, and caused an increase in LT50. Furthermore, the change in cytosolic G6PDH activity is found to be closely correlated to the degree of freezing resistance of cuttings during freezing acclimation. It is suggested that cytosolic G6PDH may be involved in the induction of freezing resistance of cuttings. [Supported by the Foundation of State-designated Base for Biology Researching and Teaching in Beijing Forestry University]  相似文献   

6.
The region of West and Central Africa is endowed with high-value fruit trees and medicinal plants, which are currently traded locally as well as on regional and international markets. Unfortunately, they are all exploited from the wild and there has been little or no focussed effort to domesticate and cultivate them. Prunus africana is one of these important medicinal plant under domestication. A series of nursery experiments were conducted to assess the effects of rooting medium (sawdust, sand and a 50:50 mixture of sand and sawdust), auxin concentration (0, 50, 100, 150, and 200 μg IBA), and leaf area (0, 5, 10, 20, and 25 cm2) on rooting success of juvenile cuttings of P. africana. The percentage of cuttings rooted was significantly greater (P < 0.05) in sawdust (80%), than in sand alone (72%) or in mixture with sawdust (71%). Leaf area also significantly affected the percentage of rooting. Leafless cuttings did not root and were all dead by week 6, but in leafy cuttings rooting ability increased proportionally with leaf area up to 20 cm2 (79%). Larger leaf cuttings (25 cm2) rooted at the same level as those of 20 cm2. The cuttings with the largest leaves also had the greatest mean number of roots per cutting (14 roots cutting−1), while those with the smallest (5 cm2) leaf area produced the fewest roots (5 roots cutting−1). The application of auxin (IBA) promoted rooting (P < 0.05) up to an optimum application of 100–200 μg IBA per cutting, but 300 μg was supraoptimal. It can be concluded that P. africana is amenable to vegetative propagation. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

7.
Net light-saturated photosynthetic rate (Amax) of field grown cocksfoot (Dactylis glomerata L.) leaves in a radiata pine (Pinus radiata D. Don) silvopastoral system (Canterbury, New Zealand) was measured at different times under severe shade (85–95 μmol m–2 s–1 photosynthetic photon flux density, PPFD) and in full sunlight (1900 μmol m–2 s–1 PPFD). The aim was to integrate individual functions for Amax against air temperature (2 to 37 oC), water status, expressed as pre-dawn leaf water potential (ψlp) (-0.01 to −1.6 MPa), herbage nitrogen (N) (1.5 to 5.9%), regrowth duration (20 to 60 days) and time under shade (1 to 180 min) into a multiplicative model. The highest Amax value obtained was 27.4 μmol CO2 m–2 s–1 in non-limiting conditions with full sunlight. This value was defined as standardised dimensionless Amaxs = 1 for comparison of factor effects. The canopy temperature of the cocksfoot sward was up to 7.4 oC cooler than air temperature for plants under shade. Therefore, canopy temperature was used to predict Amax. The only interaction was between time under severe shade (5% of the open PPFD) and water stress (ψlp = −0.4 to −1.3 MPa) and this was included in the model. Validation of this model indicated 78% of the variation in Amax could be accounted for using these five factors by the addition of the interaction function. This model could be used to assist the prediction of pasture growth in silvopastoral systems through incorporation into a canopy photosynthesis model. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

8.
In order to clarify the occurrence of natural enemies of Juglans sigillata aphid, find scientific guidance for prediction and utilizing natural enemies to prevent and control of aphids, this study established a monitoring experimental area to study the population dynamics and the occurrence of natural enemies.The results showed that the peak period of aphid occurrence was from late May to early June, and there was only one peak period throughout the year. The main natural enemies were Harmonia axyridis, Coccinella septempunctata, Propylaea japonica (Thunberg), Episyrphus balteatus De Geer, Aphidius gifuensis Ashmead and Semachrysa. The Coccinellidae was the dominant natural enemy, and Harmonia axyridis was the dominant species with a dominance index of 0.494 7. The population of Harmonia axyridis had a close following effect with the population of aphids. The population number first increased with the number increase of aphids, and then decreased with the number decrease of aphids, which reached the peak later than the occurrence of aphids, and was consistent with the following effect of predator pests.  相似文献   

9.
Established method by reverse phase HPLC and electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis and hazelnut extract trace of taxol. Through the research, seven mass spectrum characteristics of taxol compounds were contested, and the taxanes database was initially established. It was found that the paciltaxel contents were 23.65 μg·mL-1 and 4.09 μg·mL-1 in leaves and stems respectively. The paciltaxel contents in different parts of hazelnut was slightly different, which were 0.61, 0.23 and 0.35 μg·mL-1 in hazel branch, leaf and leather. With these data it can quickly identify the known paclitaxel compounds in hazelnut extract, and can perform the unknown structure analysis to certain degrees. This analysis method is confirmed sensitive, reliable and fast.  相似文献   

10.
To develop a transformation protocol of Rosa hybrida ‘Samantha’ via Agrobacterium tumefaciens, the authors examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containing β-glucuronidase gene. The results indicate that explant, light condition, salt concentration and acetosyringone (AS) concentration in co-culture medium are the most important factors, and factors like co-culture temperature, co-culture period and bacteria density have a strong effect on the growth of bacteria and then T-DNA transfer. Optimized co-cultivation was performed by inoculation of embryogenic callus with bacteria at a density of OD600=0.5–0.8 for 20 min and co-culture in darkness under 23°C on medium with 1/2 MS salts and 300 μmol·L−1 AS for 3 d. [Supported by the National Natural Science Foundation of China (Grant No.30170666)]  相似文献   

11.
【目的】通过测序法分析兰考泡桐与白花泡桐和毛泡桐在叶绿体rps16序列上的遗传差异,旨在分析三者之间在叶绿体基因上的变化特点和规律,探讨其种间的遗传关系。【方法】选取兰考泡桐、白花泡桐和毛泡桐各15个样本,对其提取的DNA用PCR扩增获得特异片段,并将其纯化与测序。利用软件Clustal X 2.0对所得序列进行排序;运行MEGA 4软件,进行多序列比对,分析其序列特征,并计算出K2P遗传距离。【结果】(1)对获得的rps16序列进行测定分析,得兰考泡桐序列长度分别为932 933 bp;白花泡桐序列长度为932 bp;毛泡桐序列长度分别为916918 bp。对所得rps16序列进行排序后的长度为938 bp,平均GC含量为34.31%。3个种所代表的个体之间共有10个变异位点,占整个序列长度的1.07%。其中有9个变异位点属于碱基插入或缺失类型,占变异位点总数的90%,占整个序列长度的0.96%。有1个变异位点属于碱基替换类型,占整个变异位点总数的10%,占整个序列长度的0.11%。(2)整个rps16片段的序列共有10个变异位点,其中兰考泡桐与白花泡桐在总的变异位点上,具有一致的碱基位点9个,占总变异的90%。而兰考泡桐与毛泡桐相比,没有相同的碱基。【结论】根据三种泡桐的rps16序列的序列特征和变异位点的分析,表明在叶绿体遗传方面,兰考泡桐具有与白花泡桐更多相似的遗传物质,其亲缘关系较近。综上所述,推测兰考泡桐与白花泡桐可能来自同一母系遗传。  相似文献   

12.
[目的]探讨O_3浓度升高对两种亚热带树木幼苗的影响,并分析其敏感性差异及原因。[方法]本试验以1年生桢楠和闽楠幼苗为材料,采用开顶式气室(OTCs),研究未过滤大气、100 nmol·mol-1(E1)、150 nmol·mol-1(E2)O_3处理对光合作用、抗氧化能力和生物量的影响。[结果]研究表明:(1) O_3熏蒸主要通过非气孔限制因素降低了两树种的净光合速率(Pn)。其中对于桢楠,E2对Pn的不利影响大于E1。而对于闽楠,8月份E2的Pn大于E1,而9月份和10月份小于E1。(2) O_3熏蒸提高了两树种的抗氧化能力,表现为总酚含量和总抗氧化能力随着O_3浓度的升高逐渐升高,而E2的还原型抗坏血酸含量低于E1。(3) O_3熏蒸降低了两树种根、茎、叶及总干质量,及降低了闽楠的根茎比。[结论]O_3熏蒸降低了两树种的光合作用,提高了抗氧化能力,最终减少了生物量。根据Pn和生物量,确定闽楠较桢楠对O_3浓度升高更加敏感。两树种间的O_3敏感性差异与气孔导度和抗氧化物质的背景水平及其对O_3浓度升高的响应有关。  相似文献   

13.
崖柏属植物的核型分析   总被引：2，自引：0，他引：2       下载免费PDF全文
[目的]为了从细胞学角度探讨崖柏属植物分类与进化问题,[方法]采用根尖压片法对崖柏属5种植物进行核型研究。[结果]表明:崖柏和日本香柏的核型公式为2n=2x=22=18m(2SAT)+4sm,朝鲜崖柏、北美乔柏和北美香柏的核型公式为2n=2x=22=20m(2SAT)+2sm,5种植物的核型均属于1A类型。[结论]该属在柏科中可能处于比较进步的进化地位;通过进化趋势图分析发现,崖柏在该属中分化最晚,而朝鲜崖柏则较原始。  相似文献   

14.
[目的]研究外源ABA处理对H_2O_2胁迫下侧柏幼苗活性氧代谢系统的影响,探讨ABA调控侧柏氧化胁迫的可能作用机制。[方法]以侧柏幼苗为试验材料,采用水培方式,研究外施低浓度(0.5μmol·L~(-1))和高浓度(200μmol·L~(-1))ABA对100 mmol·L~(-1)H_2O_2胁迫下侧柏幼苗活性氧代谢的影响。[结果](1)100 mmol·L~(-1)H_2O_2胁迫48 h显著增加了侧柏幼苗叶片过氧化氢(H_2O_2)、丙二醛(MDA)、谷胱甘肽(GSH)和脯氨酸含量、抗氧化物酶(SOD和CAT)活性,而可溶性蛋白含量降低。(2)相较于高浓度200μmol·L~(-1)ABA,施加0.5μmol·L~(-1)ABA显著减少了H_2O_2胁迫下侧柏幼苗H_2O_2和MDA的积累,进一步提高了侧柏幼苗叶片SOD、POD和CAT活性,同时促进GSH、脯氨酸和可溶性蛋白的合成。(3)100 mmol·L~(-1)H_2O_2胁迫处理48 h,侧柏幼苗叶片活性氧代谢相关基因Cu/Zn-SOD、CAT、GR、APX、MDAR和GST表达水平较对照CK均有显著性提高;正常和H_2O_2胁迫下侧柏幼苗外施0.5μmol·L~(-1)ABA相较于200μmol·L~(-1)更有利于提高侧柏叶片活性氧代谢相关基因Cu/Zn-SOD、CAT、GR、APX、MDAR和GST的表达量。[结论]低浓度0.5μmol·L~(-1)ABA有效地增强抗氧化系统的防御能力,减弱幼苗的氧化胁迫和膜脂过氧化水平,从而降低活性氧对侧柏的伤害。  相似文献   

15.
The sustainability of plantation forests is closely dependent on soil nitrogen availability in short-rotation forests established on low-fertility soils. Planting an understorey of nitrogen-fixing trees might be an attractive option for maintaining the N fertility of soils. The development of mono-specific stands of Acacia mangium (100A:0E) and Eucalyptus grandis (0A:100E) was compared with mixed-species plantations, where A. mangium was planted in a mixture at a density of 50% of that of E. grandis (50A:100E). N2 fixation by A. mangium was quantified in 100A:0E and 50A:100E at age 18 and 30 months by the 15N natural abundance method and in 50A:100E at age 30 months by the 15N dilution method. The consistency of results obtained by isotopic methods was checked against observations of nodulation, Specific Acetylene Reduction Activity (SARA), as well as the dynamics of N accumulation within both species. The different tree components (leaves, branches, stems, stumps, coarse roots, medium-sized roots and fine roots) were sampled on 5–10 trees per species for each age. Litter fall was assessed up to 30 months after planting and used to estimate fine root mortality. Higher N concentrations in A. mangium tree components than in E. grandis might be a result of N2 fixation. However, no evidence of N transfer from A. mangium to E. grandis was found. SARA values were not significantly different in 100A:0E and 50A:100E but the biomass of nodules was 20–30 times higher in 100A:0E than in 50A:100E. At age 18 months, higher δ15N values found in A. mangium tree components than in E. grandis components prevented reliable estimations of the percentage of N derived from atmospheric fixation (%Ndfa). At age 30 months, %Ndfa estimated by natural abundance and by 15N dilution amounted to 10–20 and 60%, respectively. The amount of N derived from N2 fixation in the standing biomass was estimated at 62 kg N ha−1 in 100A:0E and 3 kg N ha−1 in 50A:100E by the 15N natural abundance method, and 16 kg N ha−1 in 50A:100E by the 15N dilution method. The total amount of atmospheric N2 fixed since planting (including fine root mortality and litter fall) was estimated at 66 kg N ha−1 in 100A:0E and 7 kg N ha−1 in 50A:100E by the 15N natural abundance method, and 31 kg N ha−1 in 50A:100E by the 15N dilution method. The most reliable estimation of N2 fixation was likely to be achieved using the 15N dilution method and sampling the whole plant.  相似文献   

16.
[目的]探讨南方红豆杉根际微生物草木樨中华根瘤菌(Sinorhizobium meliloti)CHW10B的溶磷特性及促生作用。[方法]利用液体发酵试验比较不同时间、碳源、氮源、碳氮比、培养温度等条件下CHW10B菌株的溶磷量,采用温室盆栽法研究该菌株对南方红豆杉生长的影响,并分析该菌株产嗜铁素、精氨酸脱羧酶、ACC脱氨酶及有机酸等成分的能力。[结果]该菌株分别在培养时间4 d、磷酸钙5.00 g·L~(-1)、初始p H值7.0、装液量1/2、NaCl为0.0 g·L~(-1)、温度30℃、碳源为葡萄糖、氮源为硫酸铵、碳氮比100∶1时溶磷能力最强。将该菌株接种于1年生南方红豆杉实生苗360 d后,苗木的地径、苗高、生物量比对照分别增长了19.53%、20.14%、25.39%。该菌株能够产嗜铁素、精氨酸脱羧酶、ACC脱氨酶(0.922 U·mg~(-1))和IAA(8.908 mg·m L~(-1)),并可分泌大量有机酸—葡萄糖酸(5 704.92μg·L~(-1))。[结论]草木樨中华根瘤菌CHW10B溶磷能力强,对南方红豆杉促生作用显著,适用于多种不同酸碱性土壤,可高效应用于南方红豆杉的人工栽培,具有被开发为生物肥料的潜力。  相似文献   

17.
[目的]为探讨苹果属植物无融合生殖分子机制。[方法]以苹果属平邑甜茶及杂种后代33#为试材,以苹果基因组CDS序列设计引物,通过PCR扩增技术克隆出SERK同源基因的cDNA全长序列,命名为MhSERK1和MhdSERK1(GenBank登录号JQ231273和JQ231272),利用实时定量RTqPCR的方法检测了这两个基因在平邑甜茶和杂种后代各组织和器官中的表达模式。[结果]序列分析显示MhSERK1和MhdSERK1编码区序列全长为1 899 bp和1 881 bp,分别编码632和626个氨基酸,其氨基酸序列与其他植物的SERK1同源基因所编码的氨基酸同源性都在80%以上,特别是与葡萄科龙眼品种同源性最高,高达92.56%,与模式植物拟南芥、烟草等植物的SERK同源基因都具有很高的同源性。实时定量PCR结果表明,在平邑甜茶和杂种后代不同组织、花器官中SERK1基因的表达量存在差异,其中在子房中的表达量最高,在营养生长的组织中表达量很低,在平邑甜茶花蕾期的子房中表达量最高。[结论]推测该基因在平邑甜茶和杂种后代的生殖发育过程中可能发挥重要作用。  相似文献   

18.
中间锦鸡儿CiDR1的克隆及干旱胁迫下的表达分析   总被引：1，自引：1，他引：0       下载免费PDF全文
[目的]研究并了解中间锦鸡儿CiDR1基因功能及其对干旱胁迫的响应,为抗性育种提供候选基因。[方法]通过RACE技术从中间锦鸡儿中克隆CiDR1基因的c DNA全长,利用生物信息学分析软件对其基因结构及功能进行分析和预测。再通过qRT-PCR技术对干旱胁迫后的幼苗中的CiDR1表达模式进行研究。[结果]从中间锦鸡儿中克隆到CiDR1基因的c DNA全长共计4 297 bp,Gen Bank登录号为KP277100。生物信息学分析表明,预测的CiDR1蛋白序列中含有1 243个氨基酸残基,具有抗病基因特征结构域TIR、NB-ARC、LRR等,其等电点为6.35,不稳定指数为42.91,不具备信号肽,为非分泌蛋白,定位于细胞质中。定量PCR检测发现,CiDR1基因在幼年期的茎中表达量较低,在成年期的叶片中表达量较高;在干旱胁迫处理后的幼苗中,CiDR1表达水平有明显下降,表明该基因的表达受干旱抑制,可能与中间锦鸡儿适应干旱相关。[结论]中间锦鸡儿在干旱胁迫后其根、茎和叶中CiDR1的表达均明显下降,表明CiDR1的表达受干旱抑制,可能与中间锦鸡儿适应干旱相关,进一步研究发现CiDR1在根、茎、叶中的表达水平可能受发育阶段调控。  相似文献   

19.
A new norlignan, (2R,3R,4S,5S)-2,4-bis(4-hydroxyphenyl)-3,5-dihydroxy-tetrahydropyran (1), together with 9 known compounds were isolated from the branches and leaves of Taxodium ascendens. Their structures were mainly determined on the basis of MS, IR, 1D and 2D NMR spectral evidences. Methanol extract showed inhibitory activity on carbonic anhydrase II with an IC50 value of 4.27 µg/ml, acetone extract and methanol extract inhibited activity of cathepsin B with IC50 values of 2.12 and 3.71 µg/ml, respectively.  相似文献   

20.
在转录组测序结果分析基础上,以山鸡椒cDNA 为模板,克隆得到山鸡椒1-脱氧木酮糖-5-磷酸还原异构酶DXR 基因cDNA 全长,以山鸡椒基因组DNA 为模板,设计引物、扩增拼接后获得山鸡椒DXR 基因全长,命名为LcDXR。序列分析表明,LcDXR cDNA 全长为1 501 bp,5'非编码区长34 bp,3'非编码区长53 bp,开放阅读框长1 413 bp,预测编码含有470个氨基酸残基的蛋白质,等电点为6.62,分子量为51.12 kD。LcDXR 基因全长为12 601 bp,其中外显子12 个,内含子11 个。对来自10个种源的LcDXR 基因编码区单核苷酸变异位点进行分析表明:在cDNA 区间内共发现10 个SNP(single nucleotide polymorphism)位点,其中有4 个单核苷酸变异导致了所编码的氨基酸的改变,为了分析氨基酸突变导致的蛋白质精细结构的变化,利用Swiss-PDB Viewer 模拟4 个突变位点氨基酸残基的替换。其中江西安远(AY)的突变Lys119Thr 引起了氢键的变化,推测可能对酶的活性产生影响。研究结果为深入研究山鸡椒脱氧木酮糖5-磷酸还原异构酶的活性和功能奠定了基础,同时为山鸡椒遗传育种提供理论依据。  相似文献   

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