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1.
用高浓度CO2气体胁迫处理药材甲Stegobium paniceum和烟草甲Lasioderma serricorne成虫,通过终点比色法测定不同处理时间下两种昆虫乙酰胆碱酯酶AChE的活力变化及其动力学响应,分析高浓度CO2气调胁迫与AChE性质之间的关系。结果表明,气调处理18h,药材甲和烟草甲比活力分别由对照1.221μmol/mg pro./30min和1.856μmol/mg pro./30min上升到2.231μmol/mgpro./30min和2.135μmol/mg pro./30min。处理6h内,二者AChE比活力均迅速升高,药材甲AChE在处理9~18h活力上升趋于平缓,而烟草甲AChE活力则在处理6~15h内稍微降低。药材甲和烟草甲分别在处理6h和9h内,其AChE动力学参数Km下降到最低点1.830μmol/L和7.632μmol/L,随着处理时间延长,其Km值回升,至18h逐步恢复至对照组水平。高浓度CO2气调胁迫处理亦导致药材甲和烟草甲AChE Vmax的改变。 相似文献
2.
研究了药材甲(Stegobium paniceum)、烟草甲(Lasioderma serricorne)和咖啡豆象(Araecerus fasciculatus)3种中药材储藏期害虫谷胱甘肽转移酶的酶活性,探讨了该酶在CO2气调胁迫下的应激反应。结果表明,药材甲、烟草甲和咖啡豆象的谷胱甘肽转移酶比活力分别为(0.339 2±0.015 5)、(0.966 0±0.259 3)nmol/(min•mg)和(0.370 2±0.032 8)nmol/(min•mg)。CO2气调对3种昆虫GSTs的影响研究结果表明,在CO2气调处理3、6、9 h时,药材甲、烟草甲和咖啡豆象成虫的GSTs酶比活力均有一定程度的升高。可见,CO2气调亚致死处理对3种实验昆虫GSTs有一定的诱导激活作用。研究结果对于储藏物害虫气调杀虫机制的分析有指导意义。 相似文献
3.
为了解NaN3诱变和盐碱胁迫对苜蓿愈伤组织生长和生理特性的影响，利用叠氮化钠（NaN3）诱变和盐碱（NaCl、NaHCO3、Na2CO3）胁迫处理“中苜1号”苜蓿愈伤组织，测定相关生长和生理指标。结果表明：随着NaN3和盐碱浓度的增加，愈伤组织的存活率降低，在NaN3浓度为3.0 mmol·L-1（处理8 d），愈伤组织存活率为54.55%，此NaN3浓度和处理时间为NaN3诱变半致死浓度；在200 mmol·L-1盐碱浓度胁迫下（胁迫12 d），愈伤组织存活率低于5%，此盐碱浓度和胁迫时间为各盐碱致死浓度；除低浓度NaCl胁迫组外，随着NaN3和盐碱胁迫浓度的增加，愈伤组织生物量降低，NaN3降低范围为60.85%~81.88%，NaCl胁迫组降低了13.90%~65.63%，NaHCO3胁迫组降低了21.71%~67.37%，Na2CO3胁迫组降低了61.54%~73.20%，盐碱对愈伤组织生物量的影响为NaCl＞NaHCO3＞Na2CO3。经NaN3诱变和盐碱胁迫，愈伤组织可溶性蛋白、可溶性糖和脯氨酸含量显著增加，MDA含量和电导率降低，NaN3、NaN3+NaCl、NaN3+Na2CO3、NaN3+ NaHCO3各处理可溶性蛋白含量分别增加了47.07%、53.57%、22.31%、47.69%，可溶性糖含量分别增加了24.79%、162.86%、58.36%、109.54%，脯氨酸含量分别增加了47.78%、162.86%、80.00%、107.25%，MDA含量分别降低了2.35%、11.17%、3.64%、5.54%，相对电导率分别降低了15.13%、17.96%、16.79%、17.28%。NaN3诱变促进愈伤组织渗透调节物质积累和降低膜的透性。 相似文献
4.
研究了药材甲（Stegobium paniceum）、烟草甲（Lasioderma serricorne）和咖啡豆象（Araecerusfasciculatus）3种中药材储藏期害虫谷胱甘肽转移酶的酶活性,探讨了该酶在CO2气调胁迫下的应激反应。结果表明,药材甲、烟草甲和咖啡豆象的谷胱甘肽转移酶比活力分别为（0．3392±0．0155）、（0．9660±0．2593）nmol／（min·mg）和（O．3702±0．0328）nmol／（min·mg）。CO2气调对3种昆虫GSTs的影响研究结果表明,在CO2气调处理3、6、9h时,药材甲、烟草甲和咖啡豆象成虫的GSTs酶比活力均有一定程度的升高。可见,CO2气调亚致死处理对3种实验昆虫GSTs有一定的诱导激活作用。研究结果对于储藏物害虫气调杀虫机制的分析有指导意义。 相似文献
5.
为了研究蚕豆在混合盐碱胁迫下的生理变化,试验采用3种盐碱胁迫方式KCl/NaCl、KCl/Na2CO3、KCl/Na2SO4处理2周,测定蚕豆鲜重、丙二醛和脯氨酸含量、抗氧化酶活性等生理指标。结果表明,随着盐碱胁迫时间的延长,蚕豆鲜重表现为先升高后下降趋势,丙二醛和脯氨酸含量呈上升趋势,其中脯氨酸含量在处理11d时急剧增加;随胁迫时间的延长,4种抗氧化酶SOD、POD、CAT、APX活性增加幅度呈先升高后降低趋势,其中处理11d时,酶活性增加幅度顺序是SOD＞ CAT＞ APX或POD。结论:蚕豆盐碱胁迫中,SOD酶活性发挥首要作用,脯氨酸是一种重要的渗透物质。 相似文献
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为探讨多胺提高作物抗渗透胁迫的生理机制，以甜瓜品种‘齐甜2号’为材料，采用营养液法培养甜瓜幼苗，以15% PEG-6000模拟渗透胁迫，研究1 mmol·L-1亚精胺（Spd）对不同渗透胁迫时间下甜瓜幼苗的生长、活性氧代谢、抗氧化酶活性、渗透调节物质和非酶抗氧化剂的影响。结果表明：渗透胁迫后4 d，与胁迫处理相比，1 mmol·L-1 Spd处理使甜瓜幼苗生物量提高11.34%，根系活力提高24.20%；叶片和根系H2O2含量、O2产生速率、MDA含量和相对电解质渗透率降低，叶片中分别降低30.72%、29.99%、29.34%和14.62%，根中分别降低29.33%、25.31%、28.23%和20.34%。Spd诱导甜瓜叶片和根系中抗氧化酶活性增加，与胁迫处理相比，叶中SOD、POD、CAT和APX抗氧化酶活性分别提高25.54%、28.70%、27.72%和28.41%，根中分别提高23.35%、27.38%、25.06%和20.34%。与渗透胁迫处理相比，Spd处理在渗透胁迫后16 d甜瓜幼苗叶片脯氨酸、可溶性糖和可溶性蛋白的含量分别增加46.51%、49.72%和40.25%；渗透胁迫后16 d幼苗叶片ASA含量增加62.50%，渗透胁迫后12 d幼苗叶片GSH含量增加37.17%，ASA/DHA和GSH/GSSG分别提高74.46%、60.95%。说明在渗透胁迫下1 mmol·L-1浓度Spd能够促进甜瓜幼苗生长，稳定生物膜系统，增强甜瓜幼苗抗氧化能力，有效缓解渗透胁迫对甜瓜幼苗伤害的作用。 相似文献
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为探讨信号分子过氧化氢(H2O2)对干旱胁迫下裸燕麦生理响应的调控机制，以砂培白燕7号幼苗为材料，研究了喷施0.01 mmol·L-1 H2O2对17.8%聚乙二醇6000模拟的干旱胁迫下叶片光合特性、活性氧代谢、渗透调节物质积累和幼苗生长的影响。结果表明：喷施H2O2后，干旱胁迫下的裸燕麦幼苗叶片叶绿素a、叶绿素b、总叶绿素、类胡萝卜素含量和叶绿素a/b较对照(喷H2O后干旱胁迫)分别提高了20.0%、12.9%、18.5%、8.1%和24.3%，叶片净光合速率、气孔导度、胞间CO2浓度和水分利用效率分别提高了20.6%、25.9%、12.0%和33.8%，蒸腾速率降低了9.7%；喷施H2O2还使干旱胁迫下裸燕麦幼苗叶片的过氧化氢酶、抗坏血酸过氧化物酶活性和抗坏血酸含量分别提高了113.5%、102.9%和13.8%，而超氧化物歧化酶、过氧化物酶活性及O—[KG-1][JX*3]·[JX-*3]2、H2O2、丙二醛含量和细胞膜相对透性分别下降了21.5%、60.4%、29.3%、38.4%、19.7%和14.2%，可溶性蛋白质、可溶性糖、游离氨基酸和脯氨酸含量分别上升了10.5%、40.3%、13.8%和17.2%，幼苗株高、根长、植株鲜重、植株干重和相对含水量分别提高了14.8%、25.0%、20.1%、11.0%和5.93 %，但谷胱甘肽含量没有显著变化。表明外源H2O2能够通过降低膜脂过氧化程度和促进渗透溶质积累，改善叶片光合功能和植株水分状况，从而减轻干旱对裸燕麦幼苗生长的抑制，增强裸燕麦的抗旱性。 相似文献
8.
研究了浓度为10%、30%、50%、70%和90%的CO2对药材甲成虫和幼虫的毒力,结果表明,不同浓度的CO2对药材甲毒力不同,其毒力随浓度升高而增强;同时,药材甲成虫和幼虫对气调胁迫敏感性不同。在10%CO2浓度下,药材甲成虫的LT50、LT99分别为22.39、92.36 h,幼虫分别为442.13、1853.56 h;在90%CO2浓度下,成虫的LT50、LT99分别为6.89、15.83 h,幼虫分别为18.76、60.58 h。另外,不同浓度CO2致死作用下,药材甲成虫体内的多糖均低于幼虫,可溶性蛋白质含量均高于幼虫(90%浓度除外)。尤其在50%、70%及90%CO2浓度致死下,药材甲成虫的多糖含量显著低于幼虫,成虫分别为7.65、9.08和10.45μg/头,幼虫分别为8.12、9.94和11.02μg/头;在30%和70%浓度下,药材甲成虫的可溶性蛋白质含量显著高于幼虫,成虫分别为127.66和155.98μg/头,幼虫分别为110.46和142.25μg/头。随着CO2浓度的升高,药材甲对多糖及蛋白质的消耗逐渐减少,但多糖的消耗总是高于蛋白质,幼虫对多糖、蛋白质的消耗也总是高于成虫。成虫和幼虫对能源物质的消耗均在10%浓度下最高,90%浓度下最低。10%浓度下,成虫对多糖和蛋白质的利用率分别为68.53%、51.02%;幼虫分别为71.87%、59.66%;90%浓度下,成虫对多糖和蛋白质的利用率分别为22.74%、7.05%;幼虫分别为33.89%、18.07%。因此,相对于蛋白质,多糖是药材甲应对气调逆境的优先能源物质;相对于成虫,幼虫对气调具有较高的耐受性。 相似文献
9.
CO2浓度升高与增温对马铃薯产量 及品质的复合影响 总被引：1，自引：0，他引：1
于2016—2017年在黄土高原半干旱区，利用新型开顶式气室（OTC），开展CO2浓度升高与大气增温对马铃薯产量及品质的影响试验，研究CO2浓度增加和温度升高对马铃薯发育过程、产量及品质的复合影响。结果表明：增温+CO2浓度升高复合处理的叶片净光合速率比增温处理高36.3%，较对照高34.7%；马铃薯叶片的水分利用率表现为增温+CO2浓度升高复合处理高于对照47.4%,高于单独增温处理43.4%;马铃薯实际产量和块茎蛋白质含量表现为CO2浓度上升+增温处理高于对照12.9%、37.0%，高于单独增温处理59.7%、6.3%。在增温的同时增加CO2浓度，使得马铃薯叶片净光合速率增加，水分利用率增大，有机物积累增多，经济产量提高。 相似文献
10.
在盆栽条件下，以耐冷性不同的2个小豆(Vigna angularis）品种龙小豆4号（耐冷型）和天津红（冷敏型）为材料，于苗期在人工气候室进行低温（4℃，持续12 h）及预喷施外源脱落酸（abscisic acid，ABA）处理，旨在研究苗期低温胁迫下外源ABA对小豆叶片逆境生理指标（H22O22、丙二醛（MDA）、抗氧化酶活性、可溶性物质）和产量因子的调控效应。结果表明：幼苗期低温处理12 h后，龙小豆4号和天津红小豆叶片H22O22含量分别提高34.82%和27.10%；MDA含量分别提高65.56%和83.79%；SOD和POD活性分别提高6.67%和18.48%、45.53%和102.44%；脯氨酸含量分别提高33.12%和24.41%。低温处理导致龙小豆4号百粒重下降2.58%，与喷施清水相比，低温处理12 h后天津红的单株荚数和百粒重分别下降18.55%和10.61%(P＜0.05）。喷施外源ABA具有抵御低温的作用，外源ABA促进龙小豆4号和天津红叶片脯氨酸含量提高9.13%和19.76%；H22O22含量降低7.63%和8.36%；MDA含量降低10.18%和20.27%；SOD和POD活性分别提高9.06%和13.57%、8.26%和28.51%，同时百粒重分别提高8.56%和8.92%。低温胁迫下，不同品种小豆的生理应激存在显著差异；喷施外源ABA能够有效缓解低温胁迫对小豆的伤害。 相似文献
11.
三种植物次生物质对B型烟粉虱羧酸酯酶活性及其对药剂敏感度的影响 总被引：1，自引：1，他引：0
采用人工饲料添加法,研究了不同浓度的槲皮素、2-十三烷酮和葫芦素B 3种植物次生物质对B型烟粉虱Bemisia tabaci羧酸酯酶(CarE)活性的影响,同时比较研究了用3种植物次生物质处理后敌敌畏、脱叶磷、灭多威、乐果和氧乐果5种药剂对CarE 的抑制中浓度(I50)的变化。结果表明: 0.01~1.0 mg/mL的槲皮素对B型烟粉虱成虫CarE活性均具有明显的诱导增加作用,最高为对照的4.32倍; 0.1和0.5 mg/mL的2-十三烷酮处理使CarE活性比对照分别下降了22%和58%; 0.75~30.0 mg/L的葫芦素B对烟粉虱CarE活性均表现为抑制作用;用1.0 mg/mL的槲皮素处理24 h后,敌敌畏和脱叶磷对烟粉虱CarE的I50值分别增加为对照的20.05和3.16倍; 3.75 mg/L 的葫芦素B处理后,敌敌畏和灭多威对CarE 的I50值分别增加为对照的25.94、2.45倍; 1.0 mg/mL的2-十三烷酮处理后敌敌畏、脱叶磷和乐果对CarE 的I50值分别降低了89%、83%和90%。 相似文献
12.
为了获得极长链霉菌Streptomyces longissimus SL01菌株发酵的最佳培养基配方和发酵条件,采用单因素和正交试验法对SL01菌株的发酵液配方进行了优化,采用离体枝条和果实病斑抑制试验检测了其配方优化效果,并测定了SL01抑菌活性物质对温度、酸碱度和紫外光照射的稳定性。最终获得最佳发酵液的配方（质量分数）为:小米粉2%、酵母粉1%和MgSO4·7H2O 0.15%。以在28℃、初始pH 7.0、接种量2%、装液量50 mL/250 mL和转速180 r/min的条件下培养7 d得到的发酵液抑菌活性最强。采用优化的培养基配方发酵所得的SL01发酵液对苹果枝条和果实上的苹果树腐烂病的防效,分别比对照提高了39.86%和10.15%。所得发酵液在低于40℃、pH在6~10或紫外光照射12 h时,抑菌活性仍较为稳定。研究表明,通过培养基优化,可以显著提高SL01发酵产物的抑菌活性。 相似文献
13.
BACKGROUND: Vector‐borne plant disease management can be enhanced by deployment of antifeedants in addition to the use of broad‐spectrum neurotoxic insecticides. The effects of pymetrozine on Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, feeding behaviour, survival and transmission of Candidatus Liberibacter asiaticus (Las), the presumed causal pathogen of huanglongbing, were investigated. RESULTS: Pymetrozine applied at 52 and 104 µg mL?1 to citrus plants [Swingle citrumelo (X Citroncirus webberi Ingram and Moore)] modified the feeding behavior of ACP and increased the amount of time spent performing non‐penetration behaviors while decreasing the time spent performing ingestion behaviors compared with the controls 1 day after treatment. However, the antifeedant effect of pymetrozine subsided 5 days after application. Pymetrozine reduced the survival of both adults and nymphs on treated plants compared with the control. However, it had a greater impact on survival of nymphs than on survival of adults. Pymetrozine applied at 52 and 104 µg mL?1 on Las‐infected ‘Valencia’ sweet orange plants [Citrus sinensis L. (Osbeck)] reduced acquisition (12 and 21% respectively) and transmission (11 and 18% respectively) of Las by feeding ACP adults compared with the controls; however, these reductions were not statistically significant. CONCLUSIONS: Pymetrozine exhibited moderate antifeedant effects by modifying the feeding behavior of ACP adults with short residual activity. The impact of pymetrozine on survival of nymphs was greater than on adults at the higher concentrations tested. Pymetrozine also reduced the acquisition and transmission of Las by feeding ACP adults up to 21 and 18%, respectively, compared with untreated controls. Copyright © 2010 Society of Chemical Industry 相似文献
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BACKGROUND: In the present investigation, the effect of Candidatus Liberibacter asiaticus (Las), a bacterium considered to be responsible for causing huanglongbing (HLB) disease in citrus, on the physiology of its vector, the Asian citrus psyllid (ACP) Diaphorina citri Kuwayama, was determined. Specifically, the effects of Las infection on the susceptibility of ACP to selected insecticides were determined. Furthermore, total protein content and general esterase activity were quantified in Las‐infected and uninfected ACP to gain insight into the possible mechanism(s) responsible for altered susceptibility to insecticides owing to Las infection. RESULTS: LC50 values were significantly lower in Las‐infected than in uninfected ACP adults for chlorpyrifos and spinetoram. Furthermore, there was a general trend towards lower LC50 values for three other insecticides for Las‐infected ACP; however, the differences were not statistically significant. Total protein content (µg mL?1) was significantly lower in Las‐infected (23.5 ± 1.3 in head + thorax; 27.7 ± 1.9 in abdomen) than in uninfected (29.7 ± 2.1 in head + thorax; 35.0 ± 2.3 in abdomen) ACP. Likewise, general esterase enzyme activity (nmol min?1 mg?1 protein) was significantly lower in Las‐infected (111.6 ± 4.5 in head + thorax; 109.5 ± 3.7 in abdomen) than in uninfected (135.9 ± 7.5 in head + thorax; 206.1 ± 23.7 in abdomen) ACP. CONCLUSION: Susceptibility of ACP to selected insecticides from five major chemistries was greater in Las‐infected than in uninfected ACP. The lower total protein content and reduced general esterase activity in Las‐infected than in uninfected ACP may partly explain the observed higher insecticide susceptibility of Las‐infected ACP. Copyright © 2010 Society of Chemical Industry 相似文献
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The effect of botanical insecticides and bacterial toxins on gut enzyme activity of larvae of the rice leaffolderCnaphalocrocis medinalis (Guenée) (Insecta: Lepidoptera: Pyralidae) was investigated. Gut enzyme activities were affected by botanical insecticides
and bacterial toxin individually and in combination. When fed a diet of rice leaves treated with botanical insecticides and
bacterial toxins, in bioassays the activities of gut tissue enzymes — acid phosphatases (ACP), alkaline phosphatases (ALP)
and adenosine triphosphatases (ATPase) — of rice leaffolder larvae were affected. When combined, the effect was more severe
at a low concentration. Larvae that were chronically exposed to botanical insecticides and bacterial toxins showed a reduction
in weight (59–89%) and exhibited a significant reduction in ACP, ALP and ATPase activities. The combination ofBacillus thuringiensis kurstaki and botanical insecticides caused a decrease of twofold in enzyme activity even at reduced concentration. A synergistic effect
was found when botanical insecticides and bacterial toxins were combined at low doses. These effects were most pronounced
in early instars. Clear dose-response relationships were established with respect to enzyme activity. In conclusion: (i) biopesticides
are relatively safe and biodegradable; (ii) a synergistic effect of botanical insecticides and bacterial toxins was found;
(iii) less expensive, readily available and naturally occurring biopesticides could be an alternative for organic and inorganic
pesticides in controlling RLF.
http://www.phytoparasitica.org posting Sept. 28, 2004. 相似文献
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S. Senthil Nathan K. Kalaivani Paul Gene Chung 《Pesticide biochemistry and physiology》2005,81(2):113-122
The effect of neem (Azadirachta indica) limonoids azadirachtin, salannin, deacetylgedunin, gedunin, 17-hydroxyazadiradione, and deaceytlnimbin on gut enzyme activity of the rice leaffolder larvae was investigated. When fed a diet of rice leaves treated with limonoids in bioassays, gut tissue enzymes—acid phosphatases (ACP), alkaline phosphatases (ALP), and adenosine triphosphatases (ATPase) activities of rice leaffolder (Cnaphalocrocis medinalis) larvae are affected. Azadirachtin was most potent in all experiments. Larvae that were chronically exposed to limonoids showed a reduction in weight (59-89%) and exhibited a significant reduction in ACP, ALP, and ATPase activities. These results indicate neem limonoids affects gut enzyme activities. These effects are most pronounced in early instars. 相似文献
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为明确烟粉虱Bemisia tabaci(Gennadius)在大气CO2浓度和温度双因子胁迫下的生理响应,以CO2浓度和温度为作用因子,研究了4种不同组合处理下烟粉虱成虫体内超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽转移酶(GST)、乙酰胆碱酯酶(Ach E)活性的变化。结果表明:常温处理下,CO2浓度升高烟粉虱体内POD和GST活性分别增加87.6%和295%,SOD和CAT活性分别降低22.4%和28.2%;高温处理下,CO2浓度升高烟粉虱体内Ach E和GST活性分别增加103.6%和167.5%,CAT活性降低31.6%;常CO2浓度处理下,温度升高烟粉虱体内POD和SOD活性分别增加46.2%和18.2%,CAT活性降低35.8%;高CO2浓度处理下,温度升高烟粉虱体内Ach E和SOD活性分别增加75.3%和40.3%,CAT活性降低38.9%。表明CO2浓度和温度升高是导致烟粉虱体内SOD、POD、GST和Ach E活性升高的主要原因,并且SOD和POD活性变化受到CO2和温度的交互影响。烟粉虱可能通过改变体内保护酶或解毒酶的活性来适应CO2浓度和温度升高的环境。 相似文献
18.
Lectin fromGlycine max L. was extracted and purified by affinity chromatography using asialofetuin-linked porous amino-activated silica beads. The
concentration-dependent effect of lectin was studied on freshly laid eggs (0–8 h old) of the melon flyBactrocera cucurbitae (Coquillett); lectin failed to influence egg hatching. However, treating second instar larvae (64–72 h old) with increasing
concentrations of lectin significantly reduced the development period, number of pupae and number of emergingB. cucurbitae, and was negatively correlated with the increase in the lectin concentration. The LC50 value, 54μg ml−1, was calculated on the basis of adult emergence. Treatment of the larvae (64–72 h old) with the LC50 concentration resulted in a decrease in pupal weight. The activity of three hydrolase enzymes (esterases, acid and alkaline
phosphatases), one oxidoreductase (catalase) and one group transfer enzyme (glutathione S-transferase) was assayed in second
instar larvae at the LC50 concentration of lectin after exposure for 24, 48 and 72 h. The activity of esterases increased significantly (P<0.01) at the three exposure intervals, whereas the activities of the three other hydrolyses and the transferases were significantly
suppressed (P<0.01).
http://www.phytoparasitica.org posting Sept. 13, 2006. 相似文献
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采用叶片药膜法,使用亚致死浓度(LC10、LC25)的氯虫苯甲酰胺对小菜蛾Plutella xylostella(L.)3龄幼虫连续处理5代后,试虫对氯虫苯甲酰胺的敏感度分别比敏感品系下降了57.3% 和67.7%,同时对多杀菌素的敏感度也分别下降了60.2% 和51.5%,但对毒死蜱和高效氯氰菊酯的敏感度变化不明显。采用该浓度的氯虫苯甲酰胺分别处理小菜蛾3龄幼虫24、48和72 h,可诱导其羧酸酯酶(CarE)比活力上升,但对细胞色素P450 O-脱乙基酶(ECOD)、谷胱甘肽S-转移酶(GSTs)和芳基酰胺酶(AA)有明显的抑制作用;连续处理5代后,小菜蛾CarE和ECOD的比活力显著高于对照组,分别为对照组的1.16、1.40倍和1.65、1.56倍,但GSTs和AA的比活力则分别比对照下降了11.0%、27.5%和43.6%、52.5%。结果表明,小菜蛾对氯虫苯甲酰胺产生抗性的风险较高;羧酸酯酶和多功能氧化酶可能与小菜蛾对氯虫苯甲酰胺的敏感度下降有关。 相似文献