共查询到19条相似文献，搜索用时 187 毫秒
1.
试验旨在研究反季节给繁殖母羊补喂褪黑素（melatonin,MLT）及在MLT基础上补喂来曲唑（letrozole,LE）和N-甲基-D-天冬氨酸（N-methyl-D-aspartate,NMDA）对母羊血浆繁殖相关激素水平的影响,为调控母羊发情药物的开发及应用提供新思路。试验选取40只（3.0±0.5）岁经产萨福克母羊（平均体重为76.23 kg±12.25 kg）随机分为4组,每组10只,分别为对照组和试验Ⅰ、Ⅱ、Ⅲ组,分别饲喂基础日粮、基础日粮+MLT 10 mg/（只·d）、基础日粮+MLT 10 mg/（只·d）+LE 7 mg/（只·d）、基础日粮+MLT 10 mg/（只·d）+LE 7 mg/（只·d）+NMDA 300 mg/（只·d）。结果显示,与对照组相比,试验Ⅰ组母羊血浆中促卵泡素（FSH）、雌二醇（E2）、雌三醇（E3）及双氢睾酮（DHT）水平均极显著升高（P<0.01）,睾酮（T）水平显著降低（P<0.05）;试验Ⅱ组,母羊血浆MLT和LH水平均极显著升高（P<0.01）,FSH水平显著升高（P<0.05）;试验Ⅲ组,母羊血浆中MLT和促性腺激素释放激素（GnRH）水平均显著升高（P<0.05）。与试验Ⅰ组相比,试验Ⅱ组母羊血浆中MLT显著升高（P<0.05）,LH和T水平均极显著升高（P<0.01）。试验Ⅲ组与试验Ⅰ、Ⅱ组相比,FSH水平极显著降低（P<0.01）。综上所述,MLT和LE对母羊繁殖相关激素的释放有促进作用,且两者结合使用可达到调控母羊繁殖相关激素水平的效果;MLT、LE和NMDA组合应用对母羊繁殖激素调控效果不佳。 相似文献
2.
《中国畜牧兽医》2018,(12)
试验旨在研究反季节给繁殖母羊补喂褪黑素(melatonin,MLT)及在MLT基础上补喂来曲唑(letrozole,LE)和N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)对母羊血浆繁殖相关激素水平的影响,为调控母羊发情药物的开发及应用提供新思路。试验选取40只(3.0±0.5)岁经产萨福克母羊(平均体重为76.23kg±12.25kg)随机分为4组,每组10只,分别为对照组和试验Ⅰ、Ⅱ、Ⅲ组,分别饲喂基础日粮、基础日粮+MLT 10mg/(只·d)、基础日粮+MLT 10mg/(只·d)+LE 7mg/(只·d)、基础日粮+MLT 10mg/(只·d)+LE 7mg/(只·d)+NMDA 300mg/(只·d)。结果显示,与对照组相比,试验Ⅰ组母羊血浆中促卵泡素(FSH)、雌二醇(E2)、雌三醇(E3)及双氢睾酮(DHT)水平均极显著升高(P0.01),睾酮(T)水平显著降低(P0.05);试验Ⅱ组,母羊血浆MLT和LH水平均极显著升高(P0.01),FSH水平显著升高(P0.05);试验Ⅲ组,母羊血浆中MLT和促性腺激素释放激素(GnRH)水平均显著升高(P0.05)。与试验Ⅰ组相比,试验Ⅱ组母羊血浆中MLT显著升高(P0.05),LH和T水平均极显著升高(P0.01)。试验Ⅲ组与试验Ⅰ、Ⅱ组相比,FSH水平极显著降低(P0.01)。综上所述,MLT和LE对母羊繁殖相关激素的释放有促进作用,且两者结合使用可达到调控母羊繁殖相关激素水平的效果;MLT、LE和NMDA组合应用对母羊繁殖激素调控效果不佳。 相似文献
3.
本试验旨在研究补喂瓜氨酸对妊娠90 d湖羊母羊繁殖性能和泌乳性能的影响,为瓜氨酸在湖羊母羊中的应用提供科学依据。试验选取妊娠90 d的湖羊母羊50只,随机分成2组,每组25只。对照组饲喂基础饲粮,试验组在基础饲粮中添加瓜氨酸10 g/d进行补喂。试验一直进行到母羊产后45 d,记录羔羊初生重和45日龄体重。每组选取6只母羊,测定产后16 ~ 30 d泌乳量及乳成分变化,采集母羊产后第15、30、45天晨饲前颈静脉血样。结果表明：与对照组相比,试验组羔羊初生重极显著提高了19.6%（P < 0.01）|试验组羔羊45日龄体重显著提高了6%（P < 0.05）|试验组母羊产后第16 ~ 30天平均日泌乳量和总泌乳量分别极显著提高了19.6%（P < 0.01）|试验组母羊产后第1天乳蛋白率极显著提高了20%（P < 0.01）|试验组母羊产后第7 ~ 15天以及20 ~ 30天乳脂率分别极显著提高了21%和16.7%（P < 0.01）|试验组母羊产后第15天血浆中催乳素（PRL）含量极显著提高了167%（P < 0.01）,其血浆中孕酮（P4）含量显著降低207%（P < 0.05）|试验组母羊产后第15天和第45天血浆中促黄体生成素（LH）含量分别显著提高了86.3%和34.4%（P < 0.05）。综上所述,补喂瓜氨酸可提高羔羊初生重及母羊产后16 ~ 30 d日均泌乳量、乳成分和血浆中PRL、LH含量,降低血浆中P4含量,改善母羊繁殖性能和泌乳性能。
[关键词] 瓜氨酸|湖羊母羊|泌乳性能|繁殖性能 相似文献
4.
本试验旨在探究日粮中补喂N-甲基-D-天冬氨酸（NMDA）对湖羊哺乳期羔羊生长及生长轴相关激素的影响，为NMDA在哺乳期湖羊羔羊养殖中的应用提供科学依据。采用单因素多水平随机分组试验设计，选取平均体重为（5.6±1.15）kg、体况健康的7日龄羔羊32只为试验研究对象，随机分为4组，分别为CON组、1.25 NMDA组、2.5 NMDA组、5 NMDA组，每组8只。所有羔羊正常随母羊吮乳，7日龄开始补喂精料，自由采食。在此基础上，NMDA补喂水平分别为0、1.25、2.5、5 mg/kg BW只，进行为期38 d试验。结果表明：（1）在生长发育方面，补喂NMDA显著提高了湖羊哺乳期羔羊总增重，以1.25 mg/kg BW时效果最佳。（2）在血液生化指标方面，哺乳期羔羊补喂NMDA 14 d血浆中，1.25 NMDA组血浆中白蛋白含量显著高于CON组（P <0.05），提高了14.12%；各组血浆中胰岛素含量显著低于CON组（P <0.01）;5 NMDA组血浆中甘油三酯含量显著高于对照组及其他水平组（P <0.05）；各组血浆尿素含量与CON组相比分别降低了24.1%... 相似文献
5.
本试验旨在研究酵母肽和地衣芽孢杆菌对肉兔生长性能、养分表观消化率、屠宰性能、肉品质和肠道形态的影响。选取180只健康的35日龄断奶伊拉肉兔，随机分为5组，分别为空白对照组（饲喂基础饲粮）、抗生素组（饲喂基础饲粮+20 mg/kg恩拉霉素+150 mg/kg金霉素）、试验Ⅰ组（饲喂基础饲粮+250 mg/kg酵母肽）、试验Ⅱ组（饲喂基础饲粮+500 mg/kg酵母肽）、试验Ⅲ组（饲喂基础饲粮+500 mg/kg酵母肽+200 mg/kg地衣芽孢杆菌），每组6个重复，每个重复6只。预试期7 d，正试期28 d。结果显示：1）试验Ⅰ组肉兔腹泻频率显著低于空白对照组（P<0.05），且与抗生素组很接近（P<0.05）；试验Ⅰ组和抗生素组的死亡率最低；试验Ⅲ组的各项生长性能指标均劣于其他各组。2）试验Ⅰ组的粗蛋白质和粗脂肪表观消化率显著高于其他各组（P<0.05）；试验Ⅰ组和试验Ⅲ组的酸性洗涤纤维表观消化率显著高于空白对照组（P <0.05）。3）肉兔的屠宰性能在各组间均无显著差异（P>0.05），但与空白对照组相比，抗生素组和试验组的屠宰率均有升高的趋势（0.05... 相似文献
6.
《饲料工业》2016,(13)
试验旨在研究补饲日粮不同精粗比添加量对妊娠后期藏母羊生产性能及血清生化指标的影响,以确定母羊补饲日粮适宜精粗比。试验采用单因素完全随机区组设计,将900只体况良好,体重、胎次和配种时间接近的妊娠后期105 d藏母羊,随机分为3个处理,每个处理300只羊。分别饲喂3种不同精粗比(精料补充料麦青干草=46,55,64)的妊娠后期藏母羊补饲日粮,试验期45 d。结果表明:1随着精粗比例的提高,母羊分娩后失重越小,母羊的产羔率和羔羊的初生重也随之提高,羔羊的初生重Ⅱ组和Ⅲ组差异不显著(P0.05),但都极显著高于Ⅰ组(P0.01),饲喂精粗比为55和64的补饲日粮,母羊的生产性能较好;2随着精粗比例的提高,三组血清中总蛋白(TP)、白蛋白(ALB)、甘油三酯(TG)、总胆固醇(CHO)的含量差异不显著,Ⅱ组含量最高,只有血糖(GLU)含量随之升高,但Ⅱ组和Ⅲ组差异不显著(P0.05),饲喂精粗比为55补饲日粮,母羊的血清营养物质含量较高;3饲喂精粗比为55补饲日粮,经济效益最好。在本试验条件下,妊娠后期母羊补饲日粮的适宜精粗比(精料补充料和燕麦青干草日补饲量分别为75 g/只,DE和NDF水平分别为1.37 MJ和50.42 g)为55,45 d补饲成本为15.86元/只。 相似文献
7.
本试验旨在研究妊娠早期饲粮中添加N-氨甲酰谷氨酸（NCG）对母羊胚胎存活及相关血液指标的影响,并对其作用机制进行初步探讨。选择发情正常、健康状况良好的湖羊50只,随机分为2组（对照组和NCG试验组）,每组25只母羊,自配种当天开始给母羊分别饲喂基础饲粮（对照组）和基础饲粮+0.11%NCG（NCG组）,饲喂期38 d。测定妊娠第19和38天母羊血浆游离氨基酸、总一氧化氮合酶（TNOS）、内皮型一氧化氮合酶（eNOS）、诱导型一氧化氮合酶（iNOS）、一氧化氮（NO）、雌二醇、孕酮的浓度;38 d时每组各屠宰7只妊娠母羊,测定妊娠母羊的黄体数和活胎儿数等指标。结果显示,与对照组相比,妊娠第38天时,NCG组每只母羊总胎儿数增加了79.72%（P<0.05）,活胎儿数增加了79.72%（P<0.05）,胎儿形成指数增加了0.26（P<0.05）,胎水体积增加了72.89%（P<0.05）,活胎儿总重提高了71.13% （P<0.05）;NCG组妊娠第19天的甘氨酸、蛋氨酸、赖氨酸、精氨酸、NO、iNOS、eNOS和孕酮的浓度显著高于对照组（P<0.05）,NCG组妊娠第19天的瓜氨酸和脯氨酸极显著高于对照组（P<0.01）;NCG组妊娠第38天的精氨酸、脯氨酸和NO显著高于对照组（P<0.05）,NCG组妊娠第38天的iNOS和eNOS的浓度极显著高于对照组（P<0.01）。由此,妊娠早期饲粮中添加NCG提高了胚胎和胎儿的存活,增加了妊娠母羊第38天的总胎儿数和活胎儿数,改善了母羊的繁殖性能,其作用机制可能是NCG促进了母羊内源性精氨酸的合成,提高了母羊血浆精氨酸、NO和孕酮含量,改善了子宫内环境和营养供给,使之有利于胚胎着床和妊娠维持。 相似文献
8.
《饲料工业》2017,(3):18-22
为筛选能够提高辽宁绒山羊母羊繁殖性能的最适日粮锰水平,研究选择体况良好,体质量相近(34.16±0.90)kg的2.5岁母羊60只,随机分为4组,各组基础饲粮一致,锰补饲量分别是0、20、40、60 mg/kg DM,测定母羊空怀期(发情配种前)、妊娠1个月(第30 d)和妊娠4个月(第120 d)血清激素指标。结果显示,空怀期,补饲20 mg/kg DM锰组的FSH含量显著高于其他3组(P<0.05)。空怀期,补饲锰组的LH含量显著高于基础饲粮组(P<0.05);妊娠4个月,补饲60 mg/kg DM锰组血清LH含量显著高于补饲20 mg/kg DM锰组和基础饲粮组(P<0.05)。空怀期,补饲20、60 mg/kg DM锰组血清PROG含量显著高于基础饲粮组(P<0.05),妊娠1个月,补饲20 mg/kg DM锰组血清PROG含量显著高于40 mg/kg DM锰组(P<0.05)。空怀期和妊娠4个月,补饲20 mg/kg DM锰组血清MT含量显著高于基础饲粮组(P<0.05),妊娠1个月,补饲锰组的血清MT含量显著高于基础饲粮组(P<0.05)。空怀期,补饲20 mg/kg DM锰组的血清PRL含量显著低于基础饲粮组(P<0.05);妊娠1个月,补饲锰组的血清PRL含量显著低于基础饲粮组(P<0.05)。空怀期和妊娠4个月,补饲40、60 mg/kg DM锰组血清E_2含量显著高于其余两组(P<0.05);妊娠1个月,补饲锰组的血清E2含量显著高于基础饲粮组(P<0.05)。结果说明,空怀期补饲锰20 mg/kg DM(日粮锰水平59.84 mg/kg DM),妊娠期补饲锰60 mg/kg DM(日粮锰水平99.84 mg/kg DM)对提高辽宁绒山羊母羊繁殖性能的效果较好。 相似文献
9.
《饲料工业》2016,(5)
试验旨在研究绒山羊母羊日粮营养水平和羔羊早期补饲对羔羊生长发育及早期断奶的影响。将20只产单羔的哺乳母羊及其所产羔羊20只随机分为2组,每组10只母羊、10只羔羊,试验组母羊参照前苏联绒山羊母羊泌乳期饲养标准进行日粮配制,羔羊10日龄开始补饲;对照组母羊及羔羊按羊场原日粮配方饲喂,羔羊50日龄补饲,试验羔羊均随母羊自由哺乳。结果表明,试验组羔羊20、30、40、50、60日龄体重及平均日增重均极显著高于对照组(P0.01);试验组羔羊的颗粒料和苜蓿干草的平均日采食量极显著高于对照组(P0.01)。试验组各阶段母羊平均日采食量均极显著高于对照组(P0.01)。试验组母羊断奶时体重比产羔后体重增加了8.21%;对照组母羊断奶时体重比产羔后体重减少了13.12%。综合分析认为,通过合理调整母羊营养水平、羔羊适时补饲,可促进羔羊生长发育,缩短断奶日龄,保障母羊良好的种用体况。 相似文献
10.
旨在研究围产期补饲精补料对戈壁地区妊娠期放牧蒙古羊母羊增重、血液生理生化指标、泌乳性能以及羔羊生长性能的影响。选取20只体况相近、平均体重为（60±5.54）kg的妊娠期蒙古羊母羊, 按平均体重分为试验组和对照组, 每组10只。对照组按照传统补饲方法补饲玉米300 g/（只·d）, 试验组补饲精补料300 g/（只·d）。补饲试验预饲期5 d, 正饲期60 d。测定试验母羊初始体重和补饲试验末体重, 计算总增重;补饲试验结束时, 采集试验母羊血液样本, 测定血液常规指标和血清生化指标;从产后第15天起使用差重法测定母羊泌乳量, 持续到产后第85天;测定羔羊的初生重、90日龄重, 计算羔羊在90日龄内的日增重。利用统计学方法对对照组和试验组的上述指标进行分析。结果表明, 在补饲试验期间, 试验组母羊与对照组母羊的总增重差异不显著（P>0.05）, 试验组母羊的体重减少幅度比对照组母羊低1.71%;试验组母羊的各项血液常规指标和血清生化指标与对照组母羊相比差异均不显著（P>0.05）;整个泌乳期试验组母羊的泌乳量都高于对照组母羊, 其中, 产羔后75 d和85 d, 试验组母羊的泌乳量显著（P<0.05）或极显著（P<0.01）高于对照组母羊;与对照组羔羊相比, 试验组羔羊的90日龄体重和90日龄内日增重有所提高（P>0.05）。综上提示, 围产期补饲精补料能够提高放牧蒙古羊母羊的产后泌乳量, 并且有助于提升羔羊日增重。 相似文献
11.
ZHAO Guo-dong WANG Gen XIE Jing-long YANG Jian-tao TANG Xue-mei ZHANG Juan-li YANG Kai-lun 《中国畜牧兽医》2017,44(11):3229-3235
This study was aimed to investigate the effects of the different compatibility of medroxyprogesterone acetate (MPA) with prostaglandin(PG), N-methyl-D-aspartate (NMDA) and letrozole (LE) on plasma related hormones levels of reproductive ewes. Thirty-nine and 3-year-old multiparity Suffolk ewes with average weight (75.51+11.55)kg were selected,and assigned into 3 groups,test group Ⅰ:Basis diet+34 mg/d MPA+1 mL/ewe PG, test group Ⅱ:Basis diet+34 mg/d MPA+600 mg/d NMDA, test group Ⅲ:Basis diet+34 mg/d MPA+ 14 mg/d LE, respectively, the trial period lasted for 18 days. The results showed as follows:On the basis of supplemental feeding MPA with PG treated,the levels of FSH in plasma of ewes were extremely significantly increased (P<0.01); Treated with NMDA, the levels of E2, MLT,FSH and LH in plasma of ewes were extremely significantly increased (P<0.01), respectively;Treated with LE, the levels of MLT,LH and T in plasma of ewes were extremely significantly increased (P<0.01), respectively. This study suggested that MPA+NMDA and MPA+LE could be used as medicines for regulate and control oestrus of ewes, and the effect of MPA+NMDA was the best. 相似文献
12.
本文采用体外细胞培养和放射免疫测定法(RIA)的方法,研究了褪黑素(MLT)对季节性繁殖的蒙古母羊垂体细胞分泌促卵泡素(FSH)和促黄体素(LH)的作用。结果表明:当单独用递增的MLT(10、100、1000、2000pg/mL)处理原代垂体细胞时,随时间的延长FSH的分泌量极显著下降(P<0.01),但对LH的基础分泌没有影响;无论用10IU/mLhCG单独刺激,还是用不同剂量MLT与10IU/mLhCG共同刺激垂体细胞,FSH和LH的分泌都极显著高于对照组(P<0.01),但与MLT的剂量没有关系。 相似文献
13.
为揭示FSHβ和LHβ基因在小尾寒羊下丘脑-垂体-卵巢轴(HPOA)中的表达规律,深入了解其对小尾寒羊多羔的作用,本研究采用实时荧光定量PCR技术对6只小尾寒羊(FecB++型单、多羔羊各3只)的生殖组织及脑组织中FSHβ和LHβ基因的表达差异进行分析。结果表明:FSHβ和LHβ基因在大脑、小脑、下丘脑、卵巢、子宫、输卵管和垂体7种组织中均有表达,FSHβ主要在小尾寒羊下丘脑和卵巢高表达,LHβ在垂体高表达;FSHβ基因在小尾寒羊多羔群体下丘脑、卵巢、子宫、输卵管、垂体的表达极显著高于单羔群体(P<0.01),LHβ基因在小尾寒羊多羔群体下丘脑、卵巢、子宫、输卵管、垂体、小脑、大脑表达量均极显著高于单羔群体(P<0.01)。研究结果提示,FSHβ和LHβ基因可能参与小尾寒羊多羔性状调控。 相似文献
14.
The effect of the centrally acting α-adrenoceptor agonist, clonidine, on plasma LH and FSH was studied in oestradiol-primed and unprimed ewes and in oestrous ewes. In unprimed anoestrous ewes, clonidine stimulated LH and FSH release after a lag period of 18 h, and noradrenaline intracarotid injection or i.v. infusions immediately stimulated LH release. In oestradiol-infused anoestrous ewes, clonidine produced either a delay or inhibition of the gonadotrophin surge and noradrenaline i.v. infusion advanced the LH surge. In oestrous ewes treated with clonidine, there was marked delay in the LH surge, but the magnitude of the LH and FSH surges were unaffected. Intravenous administration of α-adrenoceptor blockers, phentolamine and phenoxybenzamine, blocked the oestradiol-induced gondotrophin surge in anoestrous ewes. The effect of phenoxybenzamine on gonadotrophin surge was dose dependent in oestrous ewes. Small doses (4 mg/kg i.v.) of phenoxybenzamine delayed the synchronous LH and FSH surges. There was complete blockade of the LH surge and partial blockade of FSH surges in ewes given phenoxybenzamine (8 mg/kg i.v.) before the expected synchronous gonadotrophin surges. After this experiment, the initial rise of plasma progesterone concentrations did not occur until day 6 of oestrous cycle. Administration of phenoxybenzamine before the expected second FSH surge had no effect on the second FSH surge. Gonadotrophin release induced by gonadotrophin-releasing hormone was attenuated by phenoxybenzamine, but not by clonidine. The results suggest that the LH surge is under α-adrenergic control and the first FSH surge is under partial α-adrenergic control, but the second FSH surge is not under α-adrenergic control. The results also suggest oestradiol modulation of α-adrenergic receptor action. 相似文献
15.
H C Connor P L Houghton R P Lemenager P V Malven J R Parfet G E Moss 《Domestic animal endocrinology》1990,7(3):403-411
Beef cows (n = 64) were slaughtered to evaluate effects of dietary energy and calf removal (CR) on hypothalamic and adenohypophysial endocrine characteristics. From d 190 of gestation until parturition, cows received maintenance (ME; n = 32) or low (LE; n = 32) energy diets (ME = 100%, LE = 70% NRC recommendations). After parturition, half (n = 16) of each prepartum diet group received low (LE; n = 32) or high (HE = 130% NRC; n = 32) energy diets. At 30 d postpartum, cows were slaughtered 0 or 48 hr after CR. Hypothalami [preoptic area (POA), hypothalamus (HYP), stalk-median eminence (SME)] and pituitaries were collected. Basal and K(+)-induced release of GnRH from SME, and pituitary luteinizing hormone (LH) and follicle stimulating hormone (FSH) did not differ among groups (P greater than .05). Hypophyseal LH was correlated (P less than .01) with body condition score (BCS) at parturition and slaughter (r = .36 and .47, respectively). Prepartum LE diet increased (P less than .05) met-enkephalin in POA compared to prepartum ME (.59 +/- .05 vs. .44 +/- .04 pmol/mg) regardless of postpartum diet or suckling status. Concentrations of beta-endorphin in combined HYP + POA were decreased (P less than .05) by 48 hr CR (15.1 +/- 1.1 vs. 18.1 +/- 0.7 fmol/mg).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
16.
The study was aimed to research the effects of Oxytropis glabra DC (O.glabra DC) poisoning on reproductive organs coefficent, reproductive performance and related gene expression in Hetian sheep ewes. The sera, hypothalamus, pituitary and ovary of Hetian sheep ewes cases poisoning of O. glabra DC were collected, the organ exponent and contents of GnRH, FSH, LH, E2 and P4 in serum were measured, then the mRNA expression of reproductive genes were measured. The result demonstrated that the index of hypothalamus, pituitary and ovary were extremely significantly higher than normal sheep (P < 0.01),the follicle number on the surface of the ovaries in poisoning groups were significantly lower than normal sheep (P < 0.05). The result of hematoxylin-eosin staining showed that the nuclear of neuronal cells in hypothalamus were pyknosis and hyperchromatic; The nuclear of cells in pituitary deformation and hyperchromatic, cytoplasm decreased; The primary oocyte in ovary dissolved, disappeared, interstitial blood vessel expanded. The content of GnRH, FSH, LH, E2 and P4 in serum in poisoned Hetian sheep ewes were extremely significantly lower than normal sheep (P < 0.01). The mRNA expression levels of Kiss-1, GPR54, ERα in hypothalamus, GnRHR in pituitary and FSHR and LHR in ovary were extremely significantly lower than normal sheep (P < 0.01). The result showed that O. glabra DC poisoning could affect Hetian sheep ewes reproductive system by affecting microstructure and ultrastructure of hypothalamus-pituitary-ovarian axis. 相似文献
17.
试验旨在探讨小花棘豆（Oxytropis glabra DC）中毒对和田羊母羊繁殖器官指数、性激素水平和相关基因mRNA表达量的影响。以小花棘豆中毒和田羊母羊为研究对象,采集血清后屠宰,采集试验羊的丘脑、垂体和卵巢组织,测定其脏器系数,检测血清中促性腺激素释放激素（GnRH）、促卵泡素（FSH）、促黄体素（LH）、雌二醇（E2）和孕酮（P4）含量的变化,并检测各组织中相关繁殖基因的表达。结果显示,小花棘豆中毒和田羊母羊丘脑、垂体和卵巢指数均极显著升高（P < 0.01）,且卵巢表面卵泡数显著下降（P < 0.05）,HE染色表明丘脑神经元细胞固缩、浓染;垂体中细胞核变形、浓染,胞浆减少;卵巢中初级卵母细胞溶解、消失,间质血管扩张。小花棘豆中毒和田羊母羊血清中GnRH、FSH、LH、E2和P4含量均极显著下降（P < 0.01）,丘脑中Kiss-1、GPR54、ERα mRNA,垂体中GnRHR mRNA和卵巢中FSHR、LHR mRNA表达量均极显著下降（P < 0.01）。结果表明,小花棘豆毒性成分可通过丘脑-垂体-性腺轴影响和田羊母羊的生殖系统。 相似文献
18.
Plasma concentrations of luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin (PRL) were determined over a 24-h period using radioimmunoassay in sheep injected with corn oil (control) or various doses of zeranol or estradiol-17 beta. Injection of .333, 1 or 10 mg of zeranol caused dose-related increases (P less than .01) in plasma PRL (peak levels at 12 to 18 h) and LH (peak levels at 12 to 20 h) in ovariectomized ewes. Similarly, PRL and LH increased following doses of 33 or 100 microgram of estradiol. Before the LH surge, plasma LH levels were significantly depressed (4 to 8 h). Plasma FSH levels were significantly decreased 4 to 8 h after zeranol and estradiol injection. Slight surges of FSH were observed at times similar to those of LH, but the peak level was never greater than control levels. Injection of 1 mg of zeranol or 100 microgram of estradiol into wethers resulted in a 24-h pattern of PRL secretion not significantly different of LH concentration and significantly prolonged inhibition of FSH secretion. These results indicate similarities in the effects of zeranol and estradiol on anterior pituitary hormone secretion within groups of animals of the same sex or reproductive state. Differences in secretion and plasma concentrations of LH, FSH and PRL due to underlying sexual dimorphism are maintained and expressed even when animals are challenged with structurally different compounds of varying estrogenic potencies. 相似文献
19.
Adult Suffolk ewes (n = 14) were treated on d 10 of the estrous cycle with anti-bovine luteinizing hormone (LH) antiserum. Control ewes (n = 10) were treated with normal horse serum. Estrous behavior and the number of corpora lutea and ovarian follicles were examined at the subsequent estrous cycle. Daily plasma concentrations of progesterone (P4), follicle stimulating hormone (FSH) and estradiol were determined before and after treatment. Ewes treated with antiserum had a higher (P less than .05) ovulation rate (2.7 +/- .2) than did controls (2.1 +/- .1). No differences were found in the numbers of large (greater than 5 mm) or small (less than 5 mm) follicles between treatment groups. Estrus was delayed (P less than .025) approximately .6 d/in ewes treated with antisera. Immunoreactive FSH increased (P less than .05) within 1 d after treatment and remained higher than the controls for 5 d. Peak estradiol concentrations occurred on d 17 for treated ewes compared with peak concentrations on d 15 or 16 for control ewes. The P4 concentrations were generally less (P less than .025) in treated ewes throughout the luteal phase of the treatment cycle. These data demonstrate that ovulation rate is increased in ewes treated with LH antiserum. The marked increase in plasma FSH suggests a possible mechanism whereby ovulation rate is enhanced. 相似文献