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1.
《中国兽医学报》2019,(2):337-348
将60只昆明孕鼠随机分为3组,每组20只。A组为空白对照组,孕鼠妊娠1～14d灌胃玉米油。B组为双酚A(BPA)组,孕鼠妊娠1～7d灌胃玉米油,8～14d灌胃BPA(500mg/kg)。C组为菟丝子黄酮+BPA组,孕鼠妊娠1～7d灌胃1mg/kg菟丝子黄酮,8～14d灌胃BPA(500mg/kg)。母鼠产后21d剖杀,取子宫卵巢检测。组织学观察发现BPA导致子宫或卵巢组织结构损伤,预先给予菟丝子黄酮有一定缓解作用。免疫组化和免疫荧光方法检测均显示,BPA使母鼠AMH、StAR、Bax的表达水平显著升高,CYP11a1、Kitlg、Bcl-2的表达水平显著降低。菟丝子黄酮显著缓解BPA引起上述因子的变化和伤害,起到一定的保护作用。  相似文献   

2.
为研究黄芩苷对双酚A(Bisphenol A,BPA)所致子代小鼠生殖毒性的缓解作用。本试验将60只昆明孕鼠随机分为A、B、C共3组,每组20只。A组为对照组,孕鼠妊娠1~14d灌胃生理盐水同时饲喂普通鼠粮;B组为BPA组,孕鼠妊娠1~7d给予生理盐水,妊娠8~14d给予500mg/kg BPA;C组为黄芩苷+BPA组,孕鼠妊娠1~7d灌胃1mg/kg黄芩苷,妊娠8~14d给予500mg/kg BPA,待孕鼠自然分娩。观察记录仔鼠死亡情况。至仔鼠8周龄剖杀,分离血清,摘取睾丸或卵巢,ELISA试剂盒分析仔鼠血清睾酮(T)、雌二醇(E2)及黄体生成素(LH)水平,HE染色观察卵巢或睾丸组织结构的变化,免疫组化及免疫荧光方法检测仔鼠睾丸或卵巢Bax,Bcl-2蛋白的表达。结果显示:黄芩苷能够降低BPA致子代小鼠死亡率,升高仔鼠T(♂)、LH(♀)含量,降低仔鼠(♀)E2水平(P0.05)。HE染色结果表明,BPA组仔鼠睾丸组织损伤严重,间质细胞减少,卵巢组织结构空泡逐渐增多,颗粒数量逐渐减少。黄芩苷能改善BPA引起的睾丸及卵巢组织损伤。免疫组化结果显示,BPA对仔鼠睾丸或卵巢Bax蛋白表达无影响,但对睾丸或卵巢Bcl-2蛋白表达有降低作用。预先给于黄芩苷后能够升高仔鼠睾丸或卵巢Bcl-2蛋白表达,但对Bax蛋白表达无显著影响。免疫荧光结果发现,BPA升高仔鼠睾丸或卵巢组织中Bax阳性蛋白表达,降低Bcl-2阳性蛋白表达。黄芩苷降低睾丸或卵巢Bax蛋白表达,升高睾丸或卵巢Bcl-2蛋白表达。本试验结果表明,孕鼠妊娠期给予黄芩苷降低BPA引起的子代小鼠死亡率,平衡生殖激素水平和睾丸/卵巢中相关凋亡蛋白表达,缓解BPA染毒孕鼠对子代小鼠所致的生殖毒性。  相似文献   

3.
《中国兽医学报》2019,(11):2260-2265
为探究母鼠妊娠期暴露双酚A(bisphenol A,BPA)对子代断乳雄鼠生殖内分泌与睾丸DNA甲基化的影响。按0,2.5,5.0,10.0,20.0,40.0 mg·kg~(-1)·d~(-1 )BPA剂量在妊娠期(PND)1～17 d灌胃孕鼠,制备孕鼠BPA暴露模型。然后统计母鼠妊娠期流产率,仔鼠断乳时存活率,睾丸系数。放射免疫法检测血清中雌二醇(E_2)、睾酮(T)含量;酶联免疫吸附法检测血清中雌激素受体α(ERα)、雌激素受体β(ERβ)含量;实时定量荧光PCR法检测睾丸中DNA甲基转移酶1(Dnmt1)、DNA甲基转移酶3a(Dnmt3a)、DNA甲基转移酶3b(Dnmt3b)mRNA相对转录水平。结果显示,20.0 mg/kg BPA暴露可极显著增加断乳时仔鼠睾丸系数(P0.01);20.0,40.0 mg/kg BPA暴露可显著增加母鼠流产率(P0.05),极显著降低仔鼠存活率(P0.01);2.5,20.0 mg/kg BPA暴露可极显著提高仔鼠血清T和ERβ含量(P0.01);各BPA剂量均可极显著降低仔鼠血清雌二醇水平(P0.01),促进睾丸内Dnmt1转录,抑制Dnmt3a和Dnmt3b转录;血清中雌激素受体α含量与BPA剂量呈现非线性关系。表明BPA通过干扰子代雄鼠睾丸内DNA甲基转移酶(Dnmt)的转录,使睾酮、雌二醇及雌激素受体分泌紊乱,影响子代雄鼠的生殖机能。  相似文献   

4.
双酚A(BPA)是一种无处不在的环境雌激素,长期暴露或接触会影响动物的生殖功能。为了探究持续低剂量暴露BPA对雄鼠生殖器官和功能的影响,将妊娠0 d孕鼠随机分为7组,每组20只,分别为空白对照组,0.05 mg·kg~(-1)·d~(-1) BPA组,0.50 mg·kg~(-1)·d~(-1) BPA组,5.00 mg·kg~(-1)·d~(-1) BPA组,10.00 mg·kg~(-1)·d~(-1) BPA组,20.00 mg·kg~(-1)·d~(-1) BPA组, 50.00 mg·kg~(-1)·d~(-1) BPA组。自母鼠怀孕0 d起持续饮水染毒BPA至哺乳期结束,仔鼠21 d断奶后直接以继续饮水染毒至45日龄性成熟期,共染毒63 d。子代雄鼠于45 d处死。结果显示,染毒BPA剂量大于等于10.00 mg·kg~(-1)·d~(-1)时雄鼠血清BPA含量显著高于空白对照组(P<0.05),染毒BPA剂量大于等于20.00 mg·kg~(-1)·d~(-1)时睾丸组织BPA含量显著高于空白对照组(P<0.05)。H&E染色和睾丸器官指数测定结果显示染毒BPA剂量10.00 mg·kg~(-1)·d~(-1)以上导致睾丸生精小管萎缩,小管间隙变大,50.00 mg·kg~(-1)·d~(-1)以上导致子代雄鼠睾丸指数显著增大(P<0.05)。染毒BPA剂量在0.50 mg·kg~(-1)·d~(-1)以上时睾丸精子活力与密度相较于空白对照组均显著减少(P<0.05),而各染毒组精子畸形率均显著大于空白对照组(P<0.05)。染毒BPA剂量在0.50 mg·kg~(-1)·d~(-1)以上时睾丸生殖细胞核DNA损伤显著大于空白对照组(P<0.05)。染毒BPA剂量在0.05 mg·kg~(-1)·d~(-1)以上时睾丸雄激素受体(AR)表达量显著减少(P<0.05)。转录组测序结果显示染毒BPA可导致雄鼠睾丸剪切体U1亚基蛋白质C合成基因Snrpc和剪切体通用载体组件编码基因Hnrnpu均显著下调,使得mRNA的转录后修饰第一步即无法进行,剪切体功能受阻可能是BPA影响睾丸发育的重要原因。荧光定量PCR证实了转录组结果,并进一步证明了Hnrnpu对BPA的敏感性大于Snrpc。  相似文献   

5.
旨在了解皖南花猪睾丸组织脂联素受体mRNA与睾酮合成相关因子mRNA表达的发育性变化及其相关性,探讨脂联素与睾酮生成的关系.本研究用荧光实时定量PCR法检测0、30、45、90和180 d等不同日龄(各日龄样本数5头)皖南花猪睾丸组织脂联素受体(AdpR1、AdpR2)和LHR、CYP11A1、StAR mRNA的表达水平.结果,AdpR1、CYP11 A1和StAR mRNA的表达有极显著的发育性变化(P＜0.01),AdpR1 mRNA的表达先增加后降低,45 d达到最大值;CYP11A1和StAR mRNA的表达先升高后降低再升高,45 d达到最大表达量.LHR mRNA的表达也有显著的发育性变化(P＜0.05),整体表现为先升高后降低.AdpR1与CYP11A1 mRNA的表达量显著相关(r=0.587,P＜0.05);LHR与CYP11A1、StAR mRNA的表达量分别显著相关(r=0.528,P＜0.05;r=0.552,P＜0.05);CYP11 A1与StAR mRNA表达量极显著相关(r=0.709,P＜0.01).AdpR1mRNA在睾丸组织的高表达提示脂联素可能通过AdpR1介导对睾丸的调节作用,其发育性变化和CYP11A1 mRNA呈正相关,提示脂联素对睾酮的生成有一定的促进作用.  相似文献   

6.
为探究菟丝子黄酮(CCFs)对母鼠妊娠期暴露双酚A(BPA)导致的子代成年雌鼠生殖损伤的缓解作用,将50只妊娠母鼠随机分为5组,即空白对照组、BPA模型组、BPA+低剂量CCFs组、BPA+中剂量CCFs组、BPA+高剂量CCFs组,每组10只,分别在孕1～17 d灌胃0.2 mL含有相应剂量药物的玉米油。待仔鼠出生56日龄时,每组随机选取10只子代成年雌鼠,收集血液和卵巢,统计各组成年雌鼠的卵巢指数;检测各组子代成年雌鼠血清中促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)、黄体生成素(LH)的含量;检测每组卵巢组织中雌激素(E2)、孕酮(P4)、雌激素受体α(ERα)、雌激素受体β(ERβ)水平;检测卵巢组织活性氧(ROS)、超氧化物歧化酶(SOD)、丙二醛(MDA)活性;HE染色观察各组卵巢组织学改变;荧光定量PCR法检测卵巢组织Nrf2、NQO1、HO-1 mRNA的表达。结果显示,BPA模型组小鼠卵巢指数升高,GnRH、FSH、LH、E2和P4激素水平降低,卵巢组织中ER水平紊乱。BP...  相似文献   

7.
双酚A(BPA)是一种常见的环境类雌激素,对动物产生生殖毒性。为了探究长期双酚A暴露对卵巢发育的影响,将孕0 d昆明小鼠(8周龄,体质量25～30 g,SPF级)随机分为7组,每组4个重复,每个重复5只。A组每天饮水给予蒸馏水(0.00 mg/kg BPA)作为对照组,B组每天饮水给与0.05 mg/kg BPA,C组每天饮水给与0.50 mg/kg BPA,D组每天饮水给与5.00 mg/kg BPA,E组每天饮水给与10.00 mg/kg BPA,F组每天饮水给与20.00 mg/kg BPA,G组每天饮水给与50.00 mg/kg BPA。BPA饮水自母鼠孕0 d到仔鼠45 d性成熟,共计63 d。结果显示,BPA剂量≥0.50 mg/kg时,仔鼠血清和卵巢中BPA含量显著增加(P0.05),但仔鼠卵巢器官指数均无显著变化和病理损伤。TUNEL结果显示,染毒BPA≥10.00 mg/kg时卵巢颗粒细胞发生细胞凋亡(P0.05)。当染毒BPA剂量≥0.50 mg/kg时仔鼠卵巢ERα表达量显著增多(P0.05);低剂量BPA (0.05～10.00 mg/kg)显著降低ERβ表达,较高剂量(20.00～50.00 mg/kg)显著增加ERβ表达。转录组测序结果显示,BPA影响了卵巢核糖体通路,其中核糖体蛋白L21、Sa表达基因显著下调,核糖体蛋白L3、S2、L7a、S26、L26表达基因显著上调。这些基因的改变可能会导致胚胎生殖系统发育受阻和卵巢早熟。荧光定量PCR验证了转录组结果。本研究证实长期低剂量BPA暴露不会导致性成熟期小鼠卵巢器官指数变化和明显病理损伤,但会导致卵巢细胞凋亡和ER表达异常,BPA的生殖毒性可能与核糖体功能蛋白表达异常有关。  相似文献   

8.
《中国兽医学报》2019,(12):2422-2428
为探究母鼠妊娠期暴露双酚A(bisphenol A,BPA)对子代雌鼠生殖激素的影响及BPA与机体发生作用的分子机制。通过灌服不同剂量的BPA制造孕鼠BPA暴露模型,然后计算子代小鼠断乳时的死亡率、卵巢与子宫系数;放射免疫法检测血清中雌二醇(E_2)、孕酮(P_4)、卵泡刺激素(FSH)、黄体生成素(LH)水平;酶联免疫吸附试验(ELISA)检测血清雌激素受体α(ERα)、雌激素受体β(ERβ)含量;qPCR法检测卵巢DNA甲基转移酶1(Dnmt1),DNA甲基转移酶3A(Dnmt3A),DNA甲基转移酶3B(Dnmt3B),孕酮受体(PgR)及雌激素受体α(EsR1)mRNA表达。结果显示,子代雌鼠的相关指标与母鼠妊娠期BPA暴露存在非单调性剂量-反应关系;卵巢系数、E_2含量与BPA剂量呈现"U"型关系;子宫系数,血清P_4、FSH、LH水平,卵巢ERβ含量,卵巢Dnmt1、 Dnmt3A、 Dnmt3B mRNA相对转录水平与BPA剂量呈现"波浪式"关系;卵巢PgR、EsR1 mRNA相对转录水平与BPA剂量呈现"倒U"型关系;且BPA暴露组与空白组相比有极显著差异(P0.01)。这表明BPA可能通过增强子代雌鼠DNA甲基化酶的转录活性使生殖激素分泌紊乱,造成子代生殖损伤。  相似文献   

9.
为探究新疆伊犁鹅繁殖周期中血浆主要生殖激素、卵巢形态及卵泡发育的变化规律,试验以健康状况良好、体重相近的2岁伊犁鹅为试验对象,分别在产蛋期、就巢期及休产期各随机选取32只,采集1次血样,测定激素水平;在各时期分别选取5只采集卵巢组织,观测卵巢及卵泡发育情况。结果表明,在产蛋期,伊犁鹅的GnRH分别极显著高于就巢期和休产期16.78%和58.29%（P<0.01）;PRL显著低于就巢期13.00%（P<0.05）,极显著高于休产期28.94%（P<0.01）;FSH高于就巢期6.98%（P>0.05）,极显著高于休产期21.12%（P<0.01）;LH极显著低于就巢期8.38%（P<0.01）,极显著高于休产期16.84%（P<0.01）;E2水平分别极显著高于就巢期和休产期29.80%和112.40%（P<0.01）;P4水平分别极显著高于就巢期和休产期28.89%和30.34%（P<0.01）。产蛋期伊犁鹅的卵巢体积和重量均极显著大于就巢期和休产期（P<0.01）。产蛋期伊犁鹅成熟卵泡与就巢期和休产期相比明显较大,次级卵泡数量较多,初级卵泡数量较少;就巢期卵巢上生长及成熟卵泡发生闭锁,颗粒胞层收缩,向内凹陷,胞质出现空腔,各级卵泡出现萎缩;与就巢期相比,休产期卵巢上卵泡胞质空腔变大,向内凹陷程度进一步加深,大量原始卵泡均匀排列。由此可见,在产蛋期,伊犁鹅的GnRH、E2、LH反应活跃,对卵巢卵泡发育起主导作用;在就巢期,PRL、LH维持较高的分泌水平,协同维持就巢。  相似文献   

10.
将50只雄性昆明小鼠随机分为5组,A组（n=10）正常喂饲,B、C、D、E组（各10只）小鼠均腹腔注射50mg／kg·d的BPA,1次／d,连续注射7d,构造小鼠睾丸损伤模型,同时C、D、E组分别用100、200、400mg／kg·d浓度的Que灌胃7d,A组和B组给予等量生理盐水。记录小鼠的体征、体质量变化及试验结束时睾丸、附睾和精囊腺的质量;试剂盒检测血清中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GsH—Px）和丙二醛（MDA）的含量;酶联免疫法测定血清睾酮（T）、黄体生成素（LH）和促性腺激素释放激素（GnRH）的水平;对睾丸组织进行组织学观察。结果显示：D组200mg／kg·d小鼠体质量、各脏器质量均较B组有明显改善（P〈O．05或P〈0．01）,接近A组,C组和E组只有附睾质量变化显著（P〈0．05）;Que各剂量组血清中的SOD、GSH—Px活性均高于BPA组,差异极显著（P〈0．01）,其中D组抗氧化酶的活性最高;MDA测定结果显示,D组明显降低,差异极显著（P〈0．01）,E组也有所降低,差异显著（P〈0．05）;C、D、E组小鼠血清生殖激素水平均有一定程度的恢复,但D组各生殖激素指标均接近A组;组织学观察见各给药组睾丸生精小管生精细胞层数、精子数目均高于B组,尤以D组200mg／kg·d恢复最为明显。结果表明：Que能够促进BPA致小鼠睾丸生精功能障碍后生精功能的恢复,且最佳剂量为200mg／kg·d。  相似文献   

11.
This study was aimed to investigate the reproductive toxicity of bisphenol A (BPA) exposed to the mother on the offsprings mice. Forty pregnant Kunming mice were randomly divided into 4 groups, i.e. groups A, B, C and D with 10 mice in each group. Group A was the control group and the mice received conventional feeds, mice in groups B, C and D were given 50,500 and 2 500 mg/kg BW BPA in feedstuffs during the whole gestation period (from 1 d to parturition), respectively. The death rates of the offsprings were calculated every week. The offspring mice were sacrificed at 56 days of age (at puberty). The morphology of ovary and testicular tissues were observed with hematoxylin-eosin (HE) staining. The levels of estradiol (E2), follicle stimulating hormone (FSH), testosterone (T) in mice serum were detected with ELISA Kit. The protein levels of Bax and Bcl-2 in ovary or testicular tissues were detected with immunohistochemistry, and the StAR,CYP11a mRNA levels in testicular tissues, the AMH, Kitlg mRNA levels in ovary were measured using Real-time PCR. The results showed that exposure of BPA to the mother extremely significantly increased the mortality (P<0.01),and significantly reduced the testicular weight of offspring mice (P<0.05). Maternal exposure to BPA extremely significantly reduced the levels of T (♂) and FSH(♀) (P<0.01),and extremely significantly elevated E2 (♀) level in offspring mice (P<0.01). BPA exposure damaged the testicular with less leydig cells and ovarian tissues with more vacuoles and less corpus granules in offspring mice. Immunohistochemistry results revealed that maternal exposure to BPA increased the Bax protein level and decreased the Bcl-2 protein level of testicular and ovary tissues in offspring mice. BPA significantly reduced the StAR mRNA expression in male offsprings (P<0.05). However, the mRNA level of CYP11a in groups B and D extremely significantly decreased while group C showed an significant elevation in male offsprings (P<0.01). The expression levels of Kitlg mRNA in groups C and D were decreased extremely significantly in female offsprings (P<0.01), the AMH mRNA expression in groups C and D increased significantly (P<0.05). The conclusion indicated that pregnant mice exposed to different doses of BPA had harmful effects on survival rate in offspring mice, and impact the reproductive hormones, proteins and genes expression.  相似文献   

12.
The study was aimed to research the effects of Oxytropis glabra DC (O.glabra DC) poisoning on reproductive organs coefficent, reproductive performance and related gene expression in Hetian sheep ewes. The sera, hypothalamus, pituitary and ovary of Hetian sheep ewes cases poisoning of O. glabra DC were collected, the organ exponent and contents of GnRH, FSH, LH, E2 and P4 in serum were measured, then the mRNA expression of reproductive genes were measured. The result demonstrated that the index of hypothalamus, pituitary and ovary were extremely significantly higher than normal sheep (P < 0.01),the follicle number on the surface of the ovaries in poisoning groups were significantly lower than normal sheep (P < 0.05). The result of hematoxylin-eosin staining showed that the nuclear of neuronal cells in hypothalamus were pyknosis and hyperchromatic; The nuclear of cells in pituitary deformation and hyperchromatic, cytoplasm decreased; The primary oocyte in ovary dissolved, disappeared, interstitial blood vessel expanded. The content of GnRH, FSH, LH, E2 and P4 in serum in poisoned Hetian sheep ewes were extremely significantly lower than normal sheep (P < 0.01). The mRNA expression levels of Kiss-1, GPR54, ERα in hypothalamus, GnRHR in pituitary and FSHR and LHR in ovary were extremely significantly lower than normal sheep (P < 0.01). The result showed that O. glabra DC poisoning could affect Hetian sheep ewes reproductive system by affecting microstructure and ultrastructure of hypothalamus-pituitary-ovarian axis.  相似文献   

13.
试验旨在探讨小花棘豆（Oxytropis glabra DC）中毒对和田羊母羊繁殖器官指数、性激素水平和相关基因mRNA表达量的影响。以小花棘豆中毒和田羊母羊为研究对象,采集血清后屠宰,采集试验羊的丘脑、垂体和卵巢组织,测定其脏器系数,检测血清中促性腺激素释放激素（GnRH）、促卵泡素（FSH）、促黄体素（LH）、雌二醇（E2）和孕酮（P4）含量的变化,并检测各组织中相关繁殖基因的表达。结果显示,小花棘豆中毒和田羊母羊丘脑、垂体和卵巢指数均极显著升高（P < 0.01）,且卵巢表面卵泡数显著下降（P < 0.05）,HE染色表明丘脑神经元细胞固缩、浓染;垂体中细胞核变形、浓染,胞浆减少;卵巢中初级卵母细胞溶解、消失,间质血管扩张。小花棘豆中毒和田羊母羊血清中GnRH、FSH、LH、E2和P4含量均极显著下降（P < 0.01）,丘脑中Kiss-1、GPR54、ERα mRNA,垂体中GnRHR mRNA和卵巢中FSHR、LHR mRNA表达量均极显著下降（P < 0.01）。结果表明,小花棘豆毒性成分可通过丘脑-垂体-性腺轴影响和田羊母羊的生殖系统。  相似文献   

14.
To understand effects of Bisphenol-A (BPA) exposure on the reproductive organ across generations, we analyzed morphology of the uterus and ovary, and the methylation pattern of HOXA10 gene of the 2(nd) generation. Pregnant mice (F0) were treated with sc injection of BPA in sesame oil at various doses of 0-1,000 mg/kg Bwt on days 12-16 of gestation. Their offspring (F1) were bred by foster mice, and the offspring (F2) from F1 mice were prepared. That is, F1 mice experienced in utero BPA exposure during the developmental period of reproductive organs, while F2 mice did not at all. Using these F2 mice, the present study was carried out. Comparing to the control, the body weights in BPA exposure groups were significantly increased. Correlating with the increase of body weight, the relative weights of the ovary and uterus in each group were decreased. The histological analysis revealed expansion or emphraxis of the uterine lumen and partial loss of the uterine epithelium. Unmethylation of HOXA10 gene in the uterus was observed in the intron region. The present study suggested that BPA exposure to F0 mice could affect reproductive organ of F2 mice who were not exposed to BPA.  相似文献   

15.
This experiment was aimed to study the changes of reproductive hormone and regulation of follicle development during the reproductive cycle of Yili goose in Xinjiang. 2 years old Yili goose were chosen which were in good health and similar weight. 32 samples were randomly selected to take blood samples for hormone detection during the laying, broodiness and ceased periods, and 15 samples were randomly selected to gather ovarian tissue for the observation of the development of ovary and follicular. The results showed that during the laying period, the GnRH of the Yili goose was extremely significantly higher than the broodiness and ceased periods of 16.78% and 58.29% (P<0.01);PRL was significantly lower than the broodiness period of 13.00% (P<0.05), and was extremely significantly higher than the ceased period of 28.94% (P<0.01); FSH was higher than the broodiness period of 6.98% (P>0.05),and was extremely significantly higher than the ceased period of 21.12% (P<0.01); LH was extremely significantly lower than the broodiness period of 8.38% (P<0.01),and was extremely significantly higher than the ceased period of 16.84% (P<0.01); E2 were extremely significantly higher than that the broodiness and ceased periods of 29.80% and 112.40% (P<0.01); P4 levels were extremely significantly higher than the broodiness and ceased periods of 28.89% and 30.34% (P<0.01). The ovary volume and weight of Yili goose were extremely significantly higher than that of broodiness and ceased periods (P<0.01). In the laying period, the mature follicles of Yili goose were significantly larger than that of the broodiness and resting periods, the number of secondary follicles was more and the number of primary follicles was lesser. Ovarian growth and mature follicle atresia, granule layer shrinkage, inward depression, cytoplasmic cavity, follicular atrophy at all levels in the broodiness period. The follicle cytoplasmic cavity of the ovary was larger in the ceased period than broodiness period, and inward concave further deeper, a large number of primordial follicles evenly arranged. Thus, during the laying period, the responses of GnRH, E2 and LH of Yili goose were active and played a leading role on the development of ovarian and follicular. PRL and LH maintained a high level of secretion, cooperated to maintain the broodiness behavior during the broodiness period.  相似文献   

16.
为探讨日粮粗蛋白质水平对伊犁鹅的产蛋规律、繁殖激素分泌及生殖轴相关基因mRNA表达量的影响,本研究随机选取200只3岁伊犁鹅（年龄、饲养管理水平一致及体重相近）,随机分为4个组,每组10个重复。对照组及公鹅饲喂鹅场自配料（粗蛋白质水平11.20%）,试验组母鹅分别饲喂粗蛋白质水平为13.86%、15.20%和16.48%的日粮,其余营养指标基本一致。预试期1周,正试期9周。结果表明：①试验期内伊犁鹅的产蛋率呈波动变化,对照组伊犁鹅的产蛋率在第1~2周逐渐上升,第4~5周出现小幅度增长,其余阶段表现为下滑趋势;各试验组伊犁鹅的产蛋率在第1~2、4~5周出现较大幅度增长,且在第5周时达到产蛋高峰,此后逐渐下滑。15.20%粗蛋白质组伊犁鹅就巢率显著低于11.20%、13.86%粗蛋白质组。②与对照组相比,13.86%粗蛋白质组血清促性腺激素释放激素（GnRH）浓度极显著升高（P<0.01）,且血清雌二醇（E2）、促黄体素（LH）浓度均显著升高（P<0.05）;15.20%、16.48%粗蛋白质组血清GnRH、E2及LH浓度均极显著升高（P<0.01）,且血清促卵泡素（FSH）浓度显著升高（P<0.05）。各试验组血清催乳素（PRL）浓度均显著降低（P<0.05）。③与对照组相比,15.20%粗蛋白质组下丘脑GnRH、LHR基因表达量显著上调（P<0.05）,PRL、PRLR基因表达量显著下调（P<0.05）;垂体中LH基因表达量显著上调（P<0.05）;卵巢中LH与ESR2基因表达量显著或极显著上调（P<0.05,P<0.01）。综上所述,基于日粮粗蛋白质水平对伊犁鹅的产蛋率、就巢率、血清生殖激素浓度及生殖轴相关基因mRNA相对表达量影响的综合评估,建议产蛋期伊犁鹅日粮粗蛋白质水平为15.20%。  相似文献   

17.
本试验旨在研究莱菔硫烷(SFN)对染镉雄性小鼠生殖机能的保护作用.选择健康的清洁级雄性昆明系小鼠40只,随机分成4组,分别为对照组(H2O)、镉组(2.3 mg/kg CdCl2)、莱菔硫烷组(10 mg/kg SFN)、镉+莱菔硫烷组(10 mg/kg SFN+2.3 mg/kg CdCl2),连续给药10 d,于最后一次给药2 d后脱颈处死,检测小鼠睾丸组织病理学变化、小鼠睾丸及附睾的脏器系数、精液品质及血清睾酮浓度.同时检测睾丸组织内丙二醛(MDA)、谷胱甘肽(GSH)含量及总超氧化物歧化酶(T-SOD)活性的变化.结果表明,与对照组相比,镉组小鼠的睾丸组织发生病理学损伤,睾丸及附睾的脏器系数、精液品质及血清睾酮浓度均极显著降低(P<0.01),睾丸组织T-SOD活性、GSH含量也极显著降低(P<0.01),MDA含量极显著提高(P<0.01);莱菔硫烷组T-SOD活性和GSH含量显著升高(P<0.05),MDA含量与对照组相比差异不显著(P>0.05).与镉组相比,镉+莱菔硫烷组小鼠的精子活率、精子总数极显著增加(P<0.01),睾丸与附睾的脏器系数显著增加(P<0.05),小鼠睾丸组织GSH含量、T-SOD活性极显著提高(P<0.01),MDA含量极显著降低(P<0.01).以上结果指出莱菔硫烷对镉中毒雄性小鼠的生殖机能具有保护作用.  相似文献   

18.
The study was aimed to explore the protective effect of sulforaphane (SFN) on the reproductive function of male mice with cadmium poisoning.40 healthy clean grade male Kunming mice were randomly divided into four groups:control group (H2O),cadmium chloride group (2.3 mg/kg CdCl2),sulforaphane group (10 mg/kg SFN),sulforaphane + cadmium chloride group (10 mg/kg SFN+2.3 mg/kg CdCl2),and continuous administration for 10 d,all mice were executed by dislocated cervical vertebra at 2 d after the last administration,and then the pathologic changes of testicular tissues,organ coefficient of testicle and epididymis,sperm quality and concentration of testosterone were tested.Additionally,the contents of GSH and MDA,and the activities of T-SOD in testis were also detected at the same time. Compared with the control group,pathology damages were observed in cadmium chloride group,organ coefficient of testis and epididymis,sperm quality and levels of testosterone extremely significantly decreased (P<0.01),the activities of T-SOD and GSH content were extremely significantly decreased (P<0.01),and the concentration of MDA was extremely significantly enhanced (P<0.01).Compared with the control group,the activity of T-SOD and concentration of GSH in sulforaphane group were significantly increased (P<0.05),and the concentration of MDA was not significant different between the control group and sulforaphane group (P>0.05).While compared with the cadmium chloride group,the sperm motility rate and sperm total count in sulforaphane and cadmium chloride group were extremely significantly increased (P<0.01),the organ coefficient of testicle and epididymis was increased significantly (P<0.05),the concentration of GSH and activity of T-SOD in testicular tissue were extremely significantly increased (P<0.01),and the concentration of MDA was extremely significantly decreased (P<0.01).The results indicated that sulforaphane had the protection effect on reproduction function of male mice with cadmium poisoning.  相似文献   

19.
试验旨在研究反季节给繁殖母羊补喂褪黑素（melatonin,MLT）及在MLT基础上补喂来曲唑（letrozole,LE）和N-甲基-D-天冬氨酸（N-methyl-D-aspartate,NMDA）对母羊血浆繁殖相关激素水平的影响,为调控母羊发情药物的开发及应用提供新思路。试验选取40只（3.0±0.5）岁经产萨福克母羊（平均体重为76.23 kg±12.25 kg）随机分为4组,每组10只,分别为对照组和试验Ⅰ、Ⅱ、Ⅲ组,分别饲喂基础日粮、基础日粮+MLT 10 mg/（只·d）、基础日粮+MLT 10 mg/（只·d）+LE 7 mg/（只·d）、基础日粮+MLT 10 mg/（只·d）+LE 7 mg/（只·d）+NMDA 300 mg/（只·d）。结果显示,与对照组相比,试验Ⅰ组母羊血浆中促卵泡素（FSH）、雌二醇（E2）、雌三醇（E3）及双氢睾酮（DHT）水平均极显著升高（P<0.01）,睾酮（T）水平显著降低（P<0.05）;试验Ⅱ组,母羊血浆MLT和LH水平均极显著升高（P<0.01）,FSH水平显著升高（P<0.05）;试验Ⅲ组,母羊血浆中MLT和促性腺激素释放激素（GnRH）水平均显著升高（P<0.05）。与试验Ⅰ组相比,试验Ⅱ组母羊血浆中MLT显著升高（P<0.05）,LH和T水平均极显著升高（P<0.01）。试验Ⅲ组与试验Ⅰ、Ⅱ组相比,FSH水平极显著降低（P<0.01）。综上所述,MLT和LE对母羊繁殖相关激素的释放有促进作用,且两者结合使用可达到调控母羊繁殖相关激素水平的效果;MLT、LE和NMDA组合应用对母羊繁殖激素调控效果不佳。  相似文献   

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