共查询到18条相似文献，搜索用时 187 毫秒
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油菜素内酯（brassinosteroids,BRs）是一类重要的植物促生激素,参与调控植物生长发育。最近的研究表明,BRs能增加作物产量和增强作物抗逆性。在BRs信号转导过程中,蛋白激酶的磷酸化功能与转录因子的磷酸化和脱磷酸化过程是BRs信号重要的生化调控机制,其中起始BRs信号由胞外向胞内转导的蛋白激酶BRI1和 BAK1,以及BRs信号下游调控不同性状基因表达的转录因子BZR1和BZR2/BES1,是BRs信号途径中关键的功能基因。基于重要蛋白激酶和转录因子的蛋白结构和功能分析,通过不同氨基酸功能位点的基因定点突变和修饰技术,能实现BRs信号途径的功能研究与植物性状改良,从而提高植物对环境的适应性。综述了BRs信号途径与植物生长发育和环境胁迫的研究,期望为植物分子育种提供很好的借鉴。 相似文献
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油菜素内酯对拟南芥和水稻根毛发育的影响 总被引：1，自引：0，他引：1
油菜素内酯(Brassinosteroids,BRs)是植物生长发育所必需的一类植物激素。本研究以拟南芥和水稻为研究对象,借助BR相关突变体,通过外源施加BR及BR合成抑制剂研究BR和根毛生长发育的关系。结果表明:在双子叶模式植物拟南芥中,BR抑制根毛的生长发育;在单子叶模式植物水稻中,BR对的根毛的发育存在剂量反应,在一定的浓度范围内促进根毛的生长发育。 相似文献
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【目的】研究油菜素类固醇物质（BRs）在棉花纤维生长发育过程中的作用。【方法】通过棉花EST序列的筛选和整合，从陆地棉徐州142纤维中克隆了一个BRs合成酶基因GhDWF1。【结果】其cDNA序列全长1 849 bp，包含一个1 692 bp的开放阅读框，推导编码563个氨基酸，与水稻、玉米、豌豆、番茄和拟南芥的BRs合成酶DWARF1/DIMINUTO有较高的同源性，具有该类蛋白的典型结构。GhDWF1基因在开花当天的胚珠和纤维中表达最强，在根、幼茎和纤维发育的各个时期表达较高。与徐州142野生型相比，无绒无絮突变体胚珠中表达量较低。【结论】暗示GhDWF1基因的表达与棉花纤维的生长发育有密切关系。 相似文献
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油菜素甾醇类化合物（brassinosteroids 简称BRs）是一类多羟基甾体物质，主要由A、B、C、D四个环和侧链组成，其中B环可以是六元环或者是一个含氧内酯环。在植物中普遍存在，它们调控着植物的整个生长发育进程，本研究总结了BRs类似物的生物活性，以期为其合理开发提供理论依据。 相似文献
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从蛋白质组学角度对营养缺乏胁迫下水稻籽粒蛋白质组变化进行研究,以揭示营养缺乏胁迫下水稻籽粒蛋白质组的表达差异.水稻生育后期设清水及常规营养液培养2种处理,待水稻成熟时提取籽粒蛋白质,经双向电泳分离得到电泳图谱2张,应用Imagemaster2D Elite5.0软件对其进行蛋白质差异表达分析,共得到10个发生差异表达蛋白质点.蛋白质是细胞功能的执行者,受细胞功能或细胞环境变化的影响.因此,上述蛋白质发生变化的原因应与水稻在营养缺乏胁迫下籽粒生长发育特性有关. 相似文献
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[目的]克隆水稻黄嘌呤脱氢酶(Xanthine dehydrogenase,XDH)基因(OsXDH),分析其生物信息学特性及表达特性,为研究XDH在水稻生长发育和响应逆境胁迫中的调控机制提供理论依据.[方法]以粳稻品种日本晴为材料,采用同源克隆技术克隆OsXDH基因,应用生物信息学方法对其氨基酸序列进行分析.利用实时荧光定量PCR (qPCR)检测OsXDH基因的组织表达特性及逆境胁迫下的表达情况,并对不同转基因株系乳熟期剑叶OsXDH基因表达量、XDH活性和叶绿素含量进行比较分析.[结果]克隆获得OsXDH基因的开放阅读框序列(ORF)(GenBank登录号LOC4333171),其长度为4110 bp,编码1369个氨基酸.OsXDH蛋白分子量大小为150.23 kD,理论等电点(pI)为6.54,与小麦、高粱、玉米、谷子和油菜等作物XDH蛋白氨基酸序列的相似性分别为84.54%、84.07%、81.52%、76.35%和69.22%,表明XDH蛋白氨基酸序列具有高度保守性.OsXDH基因在水稻不同组织部位均有表达,灌浆期的表达量显著高于苗期和分蘖盛期(P<0.05),且受干旱、黑暗、高温和盐胁迫诱导高效表达.OsXDH过表达水稻转基因株系乳熟期剑叶的XDH活性和叶绿素含量高于野生型,OsXDH干扰转基因株系的XDH活性和叶绿素含量低于野生型.[结论]OsXDH基因受水稻生长发育和逆境胁迫因子诱导表达,推测其是调控水稻生长发育和响应逆境胁迫的关键基因. 相似文献
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油菜素内酯是植物体内一类重要的类固醇激素,参与调控植物生长发育过程。对拟南芥的大量研究基本阐明了BR信号转导网络中的主要通路。在BR信号转导过程中,蛋白质可逆磷酸化参与了多个重要环节的调控。该文综述了植物体内BR信号转导过程中蛋白质磷酸化的作用。 相似文献
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油菜素内酯在植物生长发育中的作用机制研究进展 总被引：2，自引：0，他引：2
油菜素内酯是一种十分重要的甾醇类激素,参与调控了植物多方面的生长发育过程。近几年,研究人员结合遗传学、基因组学、蛋白质组学和细胞生物学等方法,使油菜素内酯的研究取得了显著进展。主要针对油菜素内酯在促进植物细胞伸长、细胞分裂、生殖发育、植物光形态建成、植物抗逆性及向性生长等方面的分子机制研究进行了综述,以期为相关研究提供参考。 相似文献
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OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development 
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下载免费PDF全文 CHEN Chang-zhao WANG Ya-liang HE Meng-xing LI Zhi-wen SHEN Lan LI Qing REN De-yong HU Jiang ZHU Li ZHANG Guang-heng GAO Zhen-yu ZENG Da-li GUO Long-biao QIAN Qian ZHANG Qiang 《农业科学学报》2023,22(4):972-980
Photosynthesis occurs mainly in chloroplasts, whose development is regulated by proteins encoded by nuclear genes.Among them, pentapeptide repeat(PPR) proteins participate in organelle RNA editing. Although there are more than 450members of the PPR protein family in rice, only a few affect RNA editing in rice chloroplasts. Gene editing technology has created new rice germplasm and mutants, which could be used for rice breeding and gene function study. This study evaluated the functions of OsPPR9... 相似文献
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YE Shu-e LI Fang LI Xian-bi HONG Qi-bin ZHAI Yun-lan HU Ming-yu WEI Ting DENG Sha-sha PEI Yan LUO Ming 《农业科学学报》2015,14(10):1980-1991
Brassinosteroids(BRs), a class of steroidal phytohormones are essential for many biological processes in plant. However, little is known about their roles in fruit development. Tomato is a highly valuable vegetable and has been adopted as the model species for studying fruit growth, development, and ripening. To understand the role of endogenous BRs in the development of tomato fruit, the expression patterns of three homologues of DWF4 gene were investigated and the transgenic tomato plants were generated in which the Gh DWF4 gene from upland cotton(Gossypium hirsutum L.) was ectopically expressed. The contents of main quality components were analyzed in fruits of transgenic tomato line and non-transgenic line(control plant, CP) when the fruit was mature. Sl CYP90B3 that possesses high homology with Gh DWF4 preferentially expressed in mature fruit. Significantly higher contents of soluble sugar, soluble proteins, and vitamin C were obtained in fruit of transgenic tomato lines compared with those in the CP. Furthermore, overexpressing Gh DWF4 promoted fruit growth and ripening. The weight per fruit was increased by about 23% in transgenic lines. In addition, overexpressing Gh DWF4 promoted the germination of transgenic tomato seeds and hypocotyl elongation of seedlings. These results indicated that overexpressing Gh DWF4 gene in tomato could increase the contents of many nutrients in fruit and accelerate fruit ripening. It is suggested that increased endogenous BRs in fruit affect the growth and development of tomato fruit and therefore improved the nutrient quality of tomato. 相似文献
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冷调节蛋白是一类抗寒相关蛋白,其种类十分广泛。几种水稻冷调节蛋白也曾在水稻中被发现,但是目前深入了解的水稻冷调节蛋白种类较少。作者运用生物信息学方法,用已知的冷调节蛋白作为电子探针,预测出16种水稻新型潜在冷调节蛋白,并且使用NCBI-conserved domains、SWISS-MODEL和ExPASY ProtParam tool等在线软件预测它们的功能结构域和二、三级结构以及氨基酸组成,对其作为冷调节蛋白潜在家族成员的真实性、可靠性做出理论上的判断。分析表明,这16种水稻新型潜在冷调节蛋白,符合冷调节蛋白的一些基本的结构特征,具有其他冷调节蛋白相同的功能结构域,为这16种蛋白属于水稻冷调节蛋白家族提供了一些理论依据。 相似文献
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Brassinolide influenced rice (Oryza sativa L.) root growth in a concentration-dependent manner. Roots grown in 0.1 nmol/L BL were 28% longer than those in the control.
On the other hand, the roots grown in 1000 nmol/L BL were 65% shorter in comparison with those in the control. To investigate
how BL influences rice root growth, proteome analysis techniques were applied. After BL treatments, total proteins from rice
root were extracted separately. Extracted proteins were separated by two-dimensional polyacrylamide gel electrophoresis and
analyzed using an automated protein sequencer and mass spectrometer. There were 33 proteins that showed differences in the
accumulation levels as a result of treatments with BL. Proteins related to stress tolerance, enzymes, and cell structure were
mainly found in the root. There were many proteins regulated by other hormone or light also. Results suggest that the physiologic
functions of these proteins detected using powerful proteome analysis are implicated in root elongation triggered by BL. High-level
brassinosteriod (BR) indicated that IAA amidohydrolase, which can release active IAA from IAA pool was increased, and the
IAA level was so high that the root length was inhibited. 相似文献