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从表现出血性败血症临床症状的斑马、白唇鹿、黑鹿和长颈鹿中分离出8株多杀性巴氏杆菌（Pasteurellamuhtocida,Pm）,采用Pm种特异性的KMT1/KMT2引物分别与荚膜血清群特异性的Cap A1/Cap A2、Cap B1/CapB2、Cap D1/Cap D2引物组合来鉴定分离到的菌株,并与间接血凝试验及金黄色葡萄球菌抑制试验的结果相比较,证实PCR鉴定方法与传统的生化反应鉴定结果完全一致。荚膜PCR分型结果与间接血凝试验金黄色葡萄球菌抑制试验结果完全一致,这说明多重PCR方法可用于多杀性巴氏杆菌菌种及荚膜血清型的鉴定,我国野生草食动物多杀性巴氏杆菌病中存在多个荚膜血清型。 相似文献
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鹿源多杀巴氏杆菌荚膜分型的研究 总被引：2，自引：1，他引：1
采用Ｃａｒｔｅｒ苹膜群鉴定法将分离于我国鹿的３８株多杀巴氏杆菌鉴定为三个苹膜型,其中Ｂ型占６８．４％,Ａ型占１８．４％,Ｄ型占５．３％,３株未能确定型,占７．９,证实荚膜Ｂ型是我国鹿多杀巴氏杆菌流行的主要血清群。 相似文献
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禽源多杀性巴氏杆菌多位点序列分型研究 总被引：1，自引：0，他引：1
为了解国内禽源多杀性巴氏杆菌流行情况,对分离自18省份的84株多杀性巴氏杆菌采用荚膜多重PCR分型和多位点序列分型对其血清型和基因型进行鉴定。结果表明:禽源多杀性巴氏杆菌主要以血清A型为主,占96.4%(81/84);多位点序列分型可将禽源多杀性巴氏杆菌分为5种ST型,其中ST129为主要流行型,占94.0%(79/84)。本研究为我国禽源多杀性巴氏杆菌的流行病学监测和基因多样性提供了数据支持。 相似文献
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为了确定西南民族大学动物医学实验室分离的鸭源、猪源、牛源和山羊源多杀性巴氏杆菌(Pm),即Q1、Z1、N1、Y1株的荚膜血清型及其致病性,试验采用PCR方法对分离的这4株不同动物源多杀性巴氏杆菌的种属和荚膜血清型进行鉴定,对目的基因测序分析,并用Balb/c小鼠对分离菌的致病性进行研究。结果表明:4株菌均为多杀性巴氏杆菌;鸭源Q1株为荚膜血清A型、猪源Z1株和牛源N1株为荚膜血清B型、山羊源Y1株为荚膜血清D型;对目的基因测序后与GenBank上已公布的相应荚膜血清型比对,同源性为98%～100%;不同动物源多杀性巴氏杆菌对Balb/c小鼠的致病性研究显示,猪源荚膜血清B型多杀性巴氏杆菌对Balb/c小鼠有较强的致病力。 相似文献
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近年来,羊多杀性巴氏杆菌病在内蒙古自治区呼伦贝尔市时有发生。为了解呼伦贝尔市羊源多杀性巴氏杆菌荚膜血清型流行状况,通过建立多杀性巴氏杆菌分子生物学诊断方法,以羊病变组织为研究对象,对呼伦贝尔市羊源多杀性巴氏杆菌,进行荚膜血清群分子流行病学调查,同时应用K-B纸片琼脂扩散法,对分离菌株进行药物敏感性试验,以找到针对本地区流行株的敏感药物。结果显示:从采集的35份病羊肺组织病料中,分离出12株B群、9株D群多杀性巴氏杆菌,未分离出其他血清群;分离菌株对青霉素、链霉素耐药率最高,对氯霉素、四环素、诺氟沙星、环丙沙星敏感。结果表明,呼伦贝尔市流行的羊源多杀性巴氏杆菌以B群、D群为主,且分离菌株对青霉素、链霉素产生了较高的耐药性,需指导和监管抗菌药物的合理使用。本研究查清了本地区流行的优势多杀性巴氏杆菌血清群,找到了针对性敏感药物,这为该市羊巴氏杆菌病防控提供了有效的技术支撑,也对多杀性巴氏杆菌病流行病学监测和基因多样性研究奠定了基础。 相似文献
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本文概述了在荚膜群鉴定后,利用国际多杀性巴氏杆菌菌体型参考菌株,按照Namioka等人菌体分型方法对从我省各地分离的116株猪多杀性巴氏杆菌进行菌体型鉴定的过程。结果:02型10株、05型73株、06型5株、08型28株。依菌体型和荚膜群的鉴定结果,本省猪多杀性巴氏杆菌血清型为:5:A52株,占44.8％,8:A18株,占15.5％,6:B3株,占2.6％,2:D10株,占8.6％及5:21株、6:-2株,8:-10株。5:A在本省两个大城市和八个地区广泛存在,目前,它是引起本省猪肺疫的重要血清型。8:A在各地区分布仅次于5:A。对于过去引起流行性猪肺疫的6:B在多数地区还未分离到。据文献报道,一般2:D对猪致病力弱而不稳定。 相似文献
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hyaD为A型多杀性巴氏杆菌荚膜多糖合成相关基因,为探讨该基因对多杀性巴氏杆菌毒力及其免疫保护特性的影响,本研究利用同源重组方法,构建了牛源A型多杀性巴氏杆菌CQ2株（PmCQ2）的hyaD基因缺失株（ΔhyaD）。结果发现,与野生株相比,ΔhyaD的荚膜产生量及其感染后在脏器中的细菌定殖量均显著下降,其毒力显著降低。细胞试验发现,ΔhyaD更易黏附于巨噬细胞,被吞噬数量显著多于野生株,致使巨噬细胞相关炎性因子表达显著上调。hyaD基因的缺失,可调控与荚膜合成、LPS合成转运、铁转运等相关的基因表达显著下调,促使相关保护性抗原基因表达显著上调。以制备的PmCQ2株和ΔhyaD株灭活苗免疫小鼠（加强免疫1次）,免疫后第21天分别采用同源和异源多杀性巴氏杆菌攻毒,ΔhyaD株免疫小鼠肺组织感染后24 h无明显或轻微病理损伤,对牛源A型、B型和F型多杀性巴氏杆菌的免疫保护率分别为100%、100%和80%,对兔源、猪源和禽源A型多杀性巴氏杆菌的免疫保护率分别为90%、100%、100%;而野生株PmCQ2除对牛源A型多杀性巴氏杆菌的保护率在80%以上外,对牛源B型和F型及兔、猪、禽源A型多杀性巴氏杆菌均无明显交叉保护作用。研究结果表明,hyaD基因可通过调控荚膜产生及毒力相关因子表达影响菌株毒力;hyaD基因缺失可调控相关交叉保护性抗原表达,赋予菌株交叉免疫保护特性。该研究为多杀性巴氏杆菌通用型疫苗的研发提供了参考。 相似文献
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河西走廊牛源A型多杀性巴氏杆菌灭活疫苗的制备及临床效果检测观察 总被引：1，自引：0，他引：1
根据牛源荚膜血清A型多杀性巴氏杆菌的培养特性,本研究采用脑心浸液肉汤（BHI）培养基静止培养24 h,每2h震荡一次的方法进行培养,将培养的A型多杀性巴氏杆菌灭活后制成A型多杀性巴氏杆菌灭活疫苗,免疫小白鼠和日本大耳白家兔.结果显示：A型灭活苗的保护率为100％;将制备的灭活疫苗进行肉牛安全性试验,均未发现过敏反应等异常情况;在河西走廊嘉峪关、酒泉、张掖、金昌和武威5市应用对网牛春（2～3月）、秋（10～11月）两季分别免疫注射制备的A型多杀性巴氏杆菌灭活疫苗,剂量为体重100 kg以上6 mL/头,100 kg以下4 mL/头的方法进行了较大规模的临床效果观察,免疫牛均未发现不良反应,实验组牛巴氏杆菌病发病、死亡率分别分别比对照组低2.4、1.99个百分点,差异极显著（p＜0.01）. 相似文献
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Pasteurella multocida serotype B:3,4 isolated from a fallow deer in England was used as a vaccine to prevent haemorrhagic septicaemia. The deer strain was less virulent for calves than typical serotype B:2 of haemorrhagic septicaemia strains. It elicited antibodies in cattle that protected mice against serotype B:2 infection. The live deer vaccine containing 2 X 10(7) viable organisms per dose was used to immunise calves. Six months after vaccination, five of six calves were protected against serotype B:2 challenge. Two calves challenged nine months after vaccination survived the same challenge. The live vaccine was more efficacious than an alum precipitated vaccine in protecting calves against B:2 challenge. 相似文献
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Pasteurella multocida belonging to somatic serotype 1 and capsular type A has been known to cause avian cholera in domestic poultry. Pasteurella multocida serotype 1 has also been isolated from raptorial birds. However, the capsular type for these raptorial isolates remains unknown. Moreover, the virulence of these raptorial isolates for domestic poultry has not been determined. The objectives of this study were to determine the capsular type of raptorial P. multocida serotype 1 isolates and to determine if these isolates were virulent for domestic chickens. Study chickens were inoculated with one of three P. multocida isolates. Isolate WESO-1 was obtained from a western screech owl (Otus kennicottii) and isolates RTHA-2 and RTHA-4 were isolated from two red-tailed hawks (Buteo jamaicensis). These isolates were given by either the oral, intravenous, or intraocular route. Control birds were given brain-heart infusion broth. The capsular serotypes of three isolates were also determined. The RTHA-2 and RTHA-4 isolates belonged to P. multocida capsular type A. The WESO-1 isolate belonged to capsular type F. Results also demonstrated that, for the isolates examined, the intraocular route did not cause mortality in chickens. There was mortality in all groups for the intravenous route. However, various mortality patterns were observed when P. multocida was given orally for the three different isolates. The RTHA-4 isolate (serotype 1:A) was the most virulent for domestic chickens. The WESO-1 isolate (serotype 1:F) was the least virulent for chickens among the raptorial isolates examined. 相似文献
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2013年5月从山东某大型长毛兔场患有以鼻炎和化脓性肺炎为主要临床特征的病免中分离到1株细菌,经细菌培养、染色镜检、生化试验、PCR鉴定、动物试验证实分离菌株为荚膜血清A型巴氏杆菌;药敏试验结果表明,分离菌株对替米考星、恩诺沙星、氟苯尼考、头孢噻呋钠、阿莫西林等药物敏感;通过设计3个试验组:巴氏杆菌本场疫苗免疫试验组、恩诺沙星防治试验组、巴氏杆菌本场疫苗免疫与恩诺沙星联用试验组,每组500只长毛兔,连续观察30 d,结果发现巴氏杆菌本场疫苗免疫与恩诺沙星联用试验组对该病的防控取得了较为理想的防治效果. 相似文献
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Amna B El Tayeb Teresa Y Morishita Elisabeth J Angrick 《Journal of veterinary diagnostic investigation》2004,16(2):121-125
The goal of this study was to characterize Pasteurella multocida isolated from rabbits. Five hundred and fifty-three apparently healthy rabbits were sampled for this study. Nasal swabs were collected from each rabbit for P. multocida isolation and identification. Isolates were further characterized by capsular and somatic antigens and genomic DNA fingerprinting. Thirty-nine P. multocida isolates were recovered from 553 rabbits (7%). Capsular typing was done by depolymerization of P. multocida capsule by Staphylococcus aureus hyaluronidase and by disc diffusion with mucopolysaccharidase enzymes (heparinase III, chondroitinase AC, and hyaluronidase). Thirty-one (79%) of the isolates were capsular type A, and 8 isolates (21%) had untypable (UT) capsules. The gel-diffusion precipitin test was used to determine the somatic type of P. multocida isolates. Nineteen isolates were somatic serotype 3 (49%), 12 were serotype 1 (31%), 1 was serotype 2, 2 were serotype 5, 2 were serotype 12 with a weak reaction to antiserum raised against serotype 7 (5%), and 1 was serotype 4. Two of the isolates (5%) were UT. Restriction endonuclease analysis of the DNA of the isolates revealed 7 distinct profiles by digestion with HindIII, and 12 profiles were obtained with HpaII, whereas digestion with EcoRI did not differentiate between any of the P. multocida DNA isolates studied. The DNA restriction endonuclease enzyme HpaII was found more useful for differentiating between DNA fingerprints of P. multocida rabbit isolates. However, no correlation between capsular type, somatic serotypes, and DNA fingerprints was seen in this study. 相似文献
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猪源多杀性巴氏杆菌的生物学鉴定与荚膜PCR分型 总被引：8，自引：1，他引：8
从表现猪萎缩性鼻炎临床症状的猪群中分离出13株多杀性巴氏杆菌(Pasteurella multocida,Pm),采用Pm种特异性的KMT1-KMT2引物进行PCR扩增,结果与传统的生化反应鉴定完全一致。基于对甘露醇、卫茅醇、山梨醇、海藻糖的发酵能力和产生鸟氨酸脱羧酶的特性,Q_1、Q_3、Q_6、Q_7、Q_(10)、Q_(13)和C_(48-1)鉴定为Pm多杀亚种(Pm subsp.multocida);Q_2、Q_4、Q_5、Q_8、Q_9、Q_(11)、Q_(12)鉴定为Pm败血亚种(Pm subsp.septica)。对13株Pm分离物采用A型、B型和D型引物进行PCR扩增,8株鉴定为A血清型(61.5%);5株鉴定为D血清型(38.5%);没有发现B血清型的菌株。同时对金黄色葡萄球菌抑制试验、中性吖啶黄沉淀试验与荚膜PCR分型的相关性进行了讨论。 相似文献
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The serological identity of 234 strains of Yersinia pseudotuberculosis recovered from domestic animals and birds in New Zealand was determined by slide agglutination test. Thirty strains were also examined by tube agglutination test. The strains were isolated from cattle (56), sheep (8), deer (117), goats (13), pigs (7), rabbits (6), guinea pigs (5), and aviary species of birds (22). All strains were isolated from animals or birds which had died or shown signs of ill health and amongst which diarrhoea was a common feature. Serotype I accounted for 23% (53) of strains, serotype II for 13% (30) of strains and serotype III for 64% (151) of strains. It was concluded that further investigations on the prevalence and serological identity of strains recovered from clinically healthy animals mav provide useful information in assessing the significance of various serotypes as a cause of disease in livestock. 相似文献
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为鉴定一株从雁鸭脏器内分离到的革兰氏阴性细菌,本研究对该菌进行分离培养、细菌16S rRNA序列比对、动物试验和药物敏感性试验。结果表明该分离菌与多杀性巴氏杆菌(P.mutocida)(AF224297)同源性达99.88%,毒力强,能够致死家兔、小鼠和鸡,对氧氟沙星等药物敏感。分离菌的荚膜抗原血清型特异性基因PCR产物与荚膜血清A型P.mutocida hyaD-hyaC基因同源性达99.9%,确定该菌株为荚膜血清A型P.mutocida。本研究首次从雁鸭体内分离到A型P.mutocida。 相似文献
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Thirty-eight clinical isolates of Pasteurella multocida, recovered from a continuous flow, farrow-to-finish swine herd, were characterized by capsular serotyping and restriction endonuclease analysis (REA) in order to study the epidemiology of P. multocida pneumonia. Twenty-three of the 38 isolates obtained in the study belonged to serotype A. They displayed three REA patterns after digestion with HpaII, of which one designated A-3 represented 70% of the samples. The remaining 15 isolates were serotype D. Four different REA patterns were observed in the type D isolates. The REA type D-1 was most prevalent and accounted for 47% of the serotype D isolates. All serotype A isolates were nontoxigenic, whereas five (33%) of the serotype D isolates were toxigenic. Vertical transmission of P. multocida could not be demonstrated, and was probably not a major route of infection. The results of this study suggest that strains of P. multocida virulent for pigs exist and cause swine pneumonic pasteurellosis in continuous flow herds by horizontal transmission. 相似文献