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1.
应用１２５Ｉ固相放射免疫分析法，对２２头鲁西母黄牛产后期血浆孕酮（Ｐ４）的变化作了测定，以监测黄牛产后卵巢机能。结果表明，产后（１３．６±４．４）ｄ以前，母牛血浆Ｐ４浓度仍处于基础水平〔（０．３４±０．０４）～（０．５５±０．０６）μｇ／Ｌ〕，随后，Ｐ４开始升高，出现卵巢活动。代表卵巢活动的Ｐ４曲线可分为４种类型：Ⅰ型，产后（１０．６±３．９）ｄ后，曲线呈现３个正常的黄体周期；Ⅱ型，产后（１３．６±４．４）ｄ后，首先出现１个８．５ｄ左右的短黄体周期，接着出现２个正常的黄体周期；Ⅲ型，于产后（１２．５±３．９）ｄ后，先出现１个正常的黄体周期，继之出现１个短黄体周期和１个正常黄体期；Ⅳ型，产后（１２．５±２．５）ｄ后，出现不规则的黄体周期中，最低Ｐ４水平为（０．４５±０．１５）～（０．６０±０．３８）μｇ／Ｌ，最高浓度为（２．６５±１．９５）～（４．１７±２．３５）μｇ／Ｌ。试验表明，每隔４ｄ测定１次血浆Ｐ４浓度，可准确地判定母牛的发情周期 相似文献
2.
外源激素对间情期毕格犬发情及妊娠的影响 总被引：4，自引：0，他引：4
给６只间情期雌性毕格犬注射外源激素雌酮（Ｅ１）、孕马血清促性腺激素（ＰＭＳＧ）、人绒毛膜促性腺激素（ｈＣＧ）和雌二醇（Ｅ２），结果有５只犬发情，３只受孕，产仔１４只。发情前期、发情期与自然发情犬无明显差别。发情犬血浆ＬＨ高峰出现在阴门见有血样分泌物后（１１．６±１．６）ｄ；在ＬＨ高峰期血浆孕酮（Ｐ４）明显升高，其后１２ｄ，Ｐ４含量达峰值；８０％阴道上皮细胞角化后（６．４０±０．６８）ｄ出现ＬＨ峰 相似文献
3.
山羊黄体孕酮分泌的特点 总被引：9，自引：2，他引：7
在山羊发情周期的第１０ｄ和第１７ｄ，分别给２只和１只山羊安置颈静脉导管和后腔静脉导管，以２０ｍｉｎ间隔连续采样２４ｈ。然后，将１只黄体中期羊卵巢摘除；给另１只黄体中期羊经静脉导管注射１５－甲基ＰＧＦ２ａ，每小时注射１次，每次２４０μｇ，连续７次。３只羊都继续采样。每次都同时采取颈静脉和后腔静脉血样，用ＲＩＡ法测定血浆孕酮（Ｐ４）水平。结果表明，山羊血浆Ｐ４水平没有昼夜节律；后腔静脉血浆Ｐ４水平有明显的波动，并高于颈静脉血浆Ｐ４水平（Ｐ＜０．０ｌ）；黄体溶解和卵巢摘除后，颈静脉和后腔静脉血浆的Ｐ４水平先后降到１ｎｇ／ｍＬ以下。由此证明，山羊Ｐ４是由黄体波动性地分泌的。 相似文献
4.
用电感耦合等离子发射光谱分析（ＩＣＰ一ＡＥＳ）法对８只东北虎血清中１１种金属宏量和微量元素的含量进行了测定，８只东北虎各种元素平均含量的置信区间（μ）钠为２４２±８３（ｍｇ／ｄ１）、钾为１２８土４２（ｍｇ／Ｌ）、钙为４２上１７（ｍｇ／Ｌ）、铜为Ｏ．２６±Ｏ．Ｏ９（ｍｓ／Ｌ）、镁为１１．１土４．２（ｍｇ／Ｌ）、锌为１．７５±１．３１（ｍｇ／Ｌ）、铁为１．６±Ｏ．５（ｍｇ／Ｌ）、钴为１．５±０．２５μｇ／Ｌ）、锰为１３．２土３．８μｇ／Ｌ）、钼为１Ｏ．４士Ｏ．６７（μｇ／Ｌ）和铝为３９．１±３．３（μｇ／Ｌ）。本文首次报道了多只东北虎血清多种元素的生理参考值，为东北虎多方面的研究和应用提供了科学依据。 相似文献
5.
以１７β－雌二醇－６－人血清白蛋白对新西兰公兔进行主动免疫，用ＲＩＡ检测抗体滴度和１７β－雌二醇及睾酮浓度，并检测睾丸重量和间质细胞面积。结果，试验组各兔均不同程度地产生了１７β－雌二醇抗体，其血浆１７β－雌二醇浓度极显著地低于对照组〔（２０．３±２１．６）ｎｇ／Ｌ，（１６７．７±４９．６）ｎｇ／Ｌ，Ｐ＜０．００１〕，睾酮浓度极显著地高于对照组〔（７．３±７．１）μｇ／Ｌ，（０．５２±０．２９）μｇ／Ｌ，Ｐ＜０．０１〕。每侧睾丸重量和间质细胞面积均极显著地大于对照组〔（３．９４±０．９３）ｇ和（２５２．２５±８５．７８）μｍ２，（２．９８±０．７２）ｇ和（１６１．７９±３６．４５）μｍ２，Ｐ＜０．０１和Ｐ＜０．００１〕。提示１７β－雌二醇主动免疫可望成为提高雄性动物生育力的有效途径之一 相似文献
6.
克伦特罗在猪体内的生物利用度及药物动力学研究 总被引：20，自引：1，他引：19
６头体重４７．００±４．５７ｋｇ（Ｘ±Ｓ）的健康长白和大白杂交猪，拉丁方设计试验，按４ｍｇ／ｋｇ静注、肌注和内服克伦特罗，高效液相色谱法检测血浆中药物浓度，ＭＣＰＫＰ药代动力学程序处理药时数据。静注给药的药代动力学参数是：ｔ１／２α０．６２±０．１２ｈ，ｔ１／２β４．８７±１．５６ｈ，Ｖｄ（ａｒｅａ）４．４８±０．５６ｌ／ｋｇ，ＣｌＢ０．６３±０．１１ｌ／ｋｇ／ｈ，ＡＵＣ６．３９±１．２７μｇ／ｍｌ．ｈ。肌注给药的药动学参数是：ｔ１／２Ｋａ０．２２±０．１０ｈ，ｔ１／２α０．５６±０．２１ｈ，ｔ１／２β４．２５±１．１０ｈ，ｔｍａｘ０．６０±０．１３ｈ，Ｃｍａｘ１．２７±０．３５μｇ／ｍｌ，ＡＵＣ５．４８±１．２９μｇ／ｍｌ．ｈ，Ｆ８５．４０±４．６９％。内服给药的药动学参数是：ｔ１／２Ｋａ０．２８±０．１５ｈ，ｔ１／２Ｋｅ３．１５±０．３６ｈ，ｔｍａｘ１．４６±０．１９ｈ，Ｃｍａｘ０．６５±０．１３μｇ／ｍｌ，ＡＵＣ３．９３±０．９９ｍｇ／ｌ．ｈ，Ｆ６１．０２±１０．９０％。肌注给药的生物利用度与内服比较，差异极显著（Ｐ＜０．０１）。 相似文献
7.
应用放射免疫法测定枫泾（ＦＪ）和长白（Ｌ）青年母猪妊娠２８ｄ左右外周血清和胎儿羊水、尿囊液中硫酸雌酮（Ｅ１Ｓ）的含量；观察了两品种母猪胚胎存活数。结果，ＦＪ和Ｌ母猪血清、胎儿羊水及尿囊液中Ｅ１Ｓ含量（μｇ／Ｌ）分别为５．３±０．９９、８．０±１．０、３８．０±６．７和３．６±０．４６、５．６±０．９９、６１．４±１１．７，均处于较高水平，且两品种间无显著差异。尿囊液Ｅ１Ｓ含量极显著高于羊水和母体血清含量（Ｐ＜０．０１）；胚胎存活数（个），ＦＪ和Ｌ分别为１４．５±１．３和１０．４±１．３；血清Ｅ１Ｓ浓度与其胚胎存活数呈正相关：ｒＦＪ＝０．７４２６（Ｐ＜０．０１），ｒＬ＝０．４７４７（Ｐ＜０．０５）。上述结果表明，母猪妊娠早期孕体雌激素代谢功能很强，且以Ｅ１Ｓ形式存在于血清和胎水中。母猪血清Ｅ１Ｓ浓度不仅反映早期孕体分泌雌激素的功能，而且是预测早期胚胎存活数的有价值指标 相似文献
8.
模拟发情期（０～６ｄ）母牛外周血浆雌激素和孕酮变化水平，在添加相应水平１７β雌二醇和孕酮的ＴＣＭ－１９９液中，培养间情期牛输卵管上皮细胞（ＢＯＥＣ），５％ＳＤＳ－ＰＡＧＥ分析ＢＯＥＣ分泌物，发现上皮细胞受类固醇激素作用分泌的２类蛋白质分子量与自然发情期（０～６ｄ）分泌的特异蛋白相似。证明类固醇激素可以调节和启动间情期ＢＯＥＣ的分泌活动，当雌二醇浓度高达１ｍｇ／Ｌ时，即使不加孕酮，ＢＯＥＣ仍能分泌这 相似文献
9.
应用ＲＩＡ对１５只西农萨能奶山羊在繁殖季节和非繁殖季节乳汁中的Ｐ４和１７β－Ｅ２水平变化进行研究。结果表明：５～７月份,Ｐ４水平为０９３ｎｇ／ｍｌ±０１０ｎｇ／ｍｌ（０７７～１０９ｎｇ／ｍｌ）,１７β－Ｅ２为５６３３ｐｇ／ｍｌ±４２８ｐｇ／ｍｌ（５０３３～６４２４ｐｇ／ｍｌ）。此时期西农萨能奶山羊处于非繁殖季节。８月中旬以后,试验羊的Ｐ４、１７β－Ｅ２水平均出现与繁殖季节相一致的变化。说明在舍饲条件下,西农萨能奶山羊于８月中旬进入繁殖季节;在首次发情前２ｄ,Ｐ４出现特有的小分泌峰,而在发情期其水平降至很低。配种后,妊娠羊Ｐ４水平一直维持很高,而未孕羊再次出现发情周期的Ｐ４分泌范型,在周期的第１３ｄＰ４水平达峰值（１０９３ｎｇ／ｍｌ±３４５ｎｇ／ｍｌ）。１７β－Ｅ２水平在发情期达到峰值,Ａ组（ｎ＝１２）为８５２７ｐｇ／ｍｌ±１１６１ｐｇ／ｍｌ,Ｂ组（ｎ＝３）为８８０２ｐｇ／ｍｌ±１４３１ｐｇ／ｍｌ。在发情周期的其它时间,１７β－Ｅ２在小范围内波动。 相似文献
10.
西农萨能奶山羊妊娠期外周血浆孕酮、17β──雌二醇和皮质醇的变化 总被引：1，自引：0，他引：1
采用放射免疫分析法测定了１４只西在萨能奶山羊妊娠期外周血浆孕酮（Ｐ４）、１７β－雌二醇（１７β－Ｅ２）和皮质醇（Ｆ）水平。血浆Ｐ４水平在配种当日为０．５ｎｇ／ｍｌ，以后升高，妊娠２０天时为４．７ｎｇ／ｍｌ。在妊娠３０－５０天，Ｐ４水平有所下降，最低值在４０天，为３２ｎｇ／ｍｌ，Ｐ４水平从６０天开始一直维持在６．２ｎｇ／ｍｌ左右，到妊娠结束时降低到０．６ｎｇ／ｍｌ。血浆１７ｐ－Ｅ２水平在配种后保持稳定，从妊娠４０天开始稳定上升，在妊娠结束时达到峰值７９．８ｐｇ／ｍｌ，血浆下水平在配种后呈波动式下降，到妊娠６０天变为波动式升高，并在妊娠结束时达到峰值１７ｎｇ／ｍｌ。前言西农萨能奶山羊是经过数１０年选育的高产品种，在国内享有盛名。关于西农萨能奶山羊初情期［１］、发情周期［２，３］、分娩期［４］以及非繁殖季节［５］的内分泌状况已有研究报告，但对其妊娠期的内分泌变化迄今尚无资料。为了研究西农萨能奶山羊妊娠期主要生殖激素的变化情况，给奶山羊妊娠生理学及妊娠病理学提供基础资料，我们应用放射免疫分析法（ＲＩＡ）分析了西农萨能奶山羊妊娠期外周血浆孕酮（Ｐ４）、１７β－雌二醇（１７β－Ｅ２）和皮质醇（Ｆ）的水平。材料和方法在西? 相似文献
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12.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
相似文献13.
Heartworm in dogs in Canada in 1982 总被引：8，自引：8，他引：0
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1983,24(7):227-229
14.
Ovine scrapie was first recorded in Cyprus in 1985. Subsequently four dairy goats kept in two mixed flocks with affected sheep developed characteristic clinical signs similar to those seen in sheep. Fifteen goats from the two flocks were examined histologically and neurological lesions consistent with a diagnosis of scrapie were found in the four animals and in three others which had subsequently developed early neurological signs. These lesions were similar to those of naturally-affected sheep although neuronal degeneration and vacuolation were more severe in some cases. 相似文献
15.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献16.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
17.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献18.
19.
Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
20.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献