共查询到20条相似文献，搜索用时 46 毫秒
1.
《中国兽医杂志》2015,(6)
为了对犬乳腺肿瘤分类,分析表皮生长因子受体-2(HER-2)与p53的过表达在肿瘤类型上的差异及影响,对所收集的犬乳腺肿瘤标本,进行石蜡切片、H.E.染色,划分肿瘤的病理类型,应用免疫组织化学法检测犬乳腺肿瘤中HER-2基因与p53基因的表达情况。结果 44例犬乳腺肿瘤病理组织切片中,共观察到纤维腺瘤、叶状肿瘤、导管乳头状瘤、浸润性导管癌、浸润性小叶癌(腺泡型)、小叶癌、黏液癌、髓样癌伴随淋巴细胞浸润、混合样癌9种类型;42例犬乳腺肿瘤的免疫组化结果中,HER-2在良性肿瘤中无表达,在恶性肿瘤中的表达率为38.9%,p53在犬乳腺肿瘤中的表达可达78.6%,而在恶性肿瘤中的表达率100%。结论 HER-2与p53的过表达有肿瘤类型的差异性。 相似文献
2.
《畜牧与兽医》2017,(4):106-110
为了探讨腱糖蛋白C(TNC)表达与犬乳腺肿瘤生物学关系,对23例犬乳腺肿瘤临床病例进行病理学观察及分类,然后采用免疫组化SABC方法检测TNC蛋白在23例犬乳腺肿瘤和2例犬正常乳腺组织中的表达情况。结果显示:23例病例中,良性肿瘤有7例,占30.43%;恶性肿瘤有13例,占56.52%;增生病变有3例,占13.05%。在良性肿瘤中良性混合瘤最多,为3例,其余2例为导管内乳头状腺瘤,1例复杂腺瘤及1例纤维腺瘤。恶性肿瘤中,5例未分化癌,3例癌肉瘤,2例筛状癌,2例固体癌,1例纤维肉瘤,1例混合瘤引起的癌。TNC蛋白主要表达于细胞外基质。犬恶性乳腺肿瘤中,TNC蛋白阳性表达率为46.15%(6/13);良性乳腺肿瘤中,其阳性表达率为42.85%(3/7);乳腺增生病变和正常乳腺组织中,未见其表达。研究结果表明,TNC蛋白可以为犬乳腺肿瘤的鉴别诊断提供参考。 相似文献
3.
犬的乳腺肿瘤是犬生殖系统最常见的肿瘤，占生殖系统肿瘤的82％。有良性肿瘤和恶性肿瘤之分。常发生于10～1l岁老年母犬，2岁以下的犬少发。纯种犬发病率高于杂种犬，无产仔犬高于产仔犬；肿瘤中恶性肿瘤约占40％，良性肿瘤主要有腺瘤、纤维腺瘤约占50％，此外肉瘤与混合瘤各占4％。乳腺肿瘤发生与激素有关，摘除后易复发或转移到其它脏器。治疗最佳方法是手术。结合本人实践要做好肿瘤切除几个要点，现报道如下： 相似文献
4.
5.
为探讨犬常见肿瘤的发病规律，试验收集哈尔滨地区2016-2017年宠物医院部分犬肿瘤病例71例，采用组织病理学方法对犬肿瘤进行病理学诊断，并对患病动物的品种、性别、年龄、发生部位和饮食情况与肿瘤发生关系进行统计分析。结果显示，71例确诊肿瘤病例中，恶性肿瘤28例，包括鳞状上皮细胞癌3例，基底细胞癌5例，乳腺癌14例，淋巴瘤2例，肾母细胞瘤、精原细胞癌、生殖细胞癌、侵袭性血管黏液瘤各1例；良性肿瘤43例，包括良性乳腺肿瘤15例，纤维瘤10例，平滑肌瘤5例，乳头状瘤和精原细胞瘤各3例，造釉细胞瘤、耵聍腺瘤、脂肪瘤、结节样增生、性索间质细胞瘤、颗粒细胞瘤、肛周腺瘤各1例。上述病例中，乳腺和皮肤是犬肿瘤的高发部位，分别占全部患犬的40.8%和34.0%；患病犬年龄1~16岁不等，平均发病年龄为9.1岁，7岁以后是肿瘤的高发期，中老年犬仍为主要发病群体，但低龄犬发病数量上升；各品种犬均可发生肿瘤，其中杂种犬发病率最高，其次是贵宾犬和京巴犬，且主要为乳腺肿瘤；饮食习惯主要以剩饭剩菜类和肉拌饭类为主，分别占患犬总数的39%和30%；部分肿瘤类型与犬的性别、是否绝育有关，如乳腺肿瘤多发生于未绝育的老年母犬。本研究结果为犬肿瘤的流行病学及诊断提供了参考依据，对犬肿瘤的预防和诊断有一定的借鉴意义。 相似文献
6.
为探讨犬常见肿瘤的发病规律,试验收集哈尔滨地区2016—2017年宠物医院部分犬肿瘤病例71例,采用组织病理学方法对犬肿瘤进行病理学诊断,并对患病动物的品种、性别、年龄、发生部位和饮食情况与肿瘤发生关系进行统计分析。结果显示,71例确诊肿瘤病例中,恶性肿瘤28例,包括鳞状上皮细胞癌3例,基底细胞癌5例,乳腺癌14例,淋巴瘤2例,肾母细胞瘤、精原细胞癌、生殖细胞癌、侵袭性血管黏液瘤各1例;良性肿瘤43例,包括良性乳腺肿瘤15例,纤维瘤10例,平滑肌瘤5例,乳头状瘤和精原细胞瘤各3例,造釉细胞瘤、耵聍腺瘤、脂肪瘤、结节样增生、性索间质细胞瘤、颗粒细胞瘤、肛周腺瘤各1例。上述病例中,乳腺和皮肤是犬肿瘤的高发部位,分别占全部患犬的40.8%和34.0%;患病犬年龄1~16岁不等,平均发病年龄为9.1岁,7岁以后是肿瘤的高发期,中老年犬仍为主要发病群体,但低龄犬发病数量上升;各品种犬均可发生肿瘤,其中杂种犬发病率最高,其次是贵宾犬和京巴犬,且主要为乳腺肿瘤;饮食习惯主要以剩饭剩菜类和肉拌饭类为主,分别占患犬总数的39%和30%;部分肿瘤类型与犬的性别、是否绝育有关,如乳腺肿瘤多发生于未绝育的老年母犬。本研究结果为犬肿瘤的流行病学及诊断提供了参考依据,对犬肿瘤的预防和诊断有一定的借鉴意义。 相似文献
7.
《畜牧与兽医》2015,(9):98-100
为探讨血清中肿瘤性标志物(TM)在犬肿瘤疾病的早期诊断、多种恶性肿瘤的诊断及肿瘤筛查中的应用价值,本试验选择7只健康犬作为对照组,以临床接诊的32例肿瘤患犬为试验组,分别测定犬血清中癌胚抗原(CEA)、甲胎蛋白(AFP)、糖类抗原25(CA125)、糖类抗原15-3(CA15-3)和肿瘤特异性生长因子(TSGF)等5项TM的含量。试验结果显示,与对照组相比,良性肿瘤组血清中AFP、CA125和CA15-3的含量显著升高(P0.05),恶性肿瘤组血清中AFP和CA125的含量显著升高(P0.05),TSGF极显著升高(P0.01);与良性肿瘤组相比,恶性肿瘤组血清中TSGF极显著升高(0.01);恶性肿瘤组的5项TM的阳性检出率均高于良性肿瘤组;TM联合检测其阳性检出率均高于单一TM检测阳性检出率;与乳腺及生殖器官肿瘤组相比,皮下及软组织肿瘤组血清中CA125和CA15-3含量显著降低(P0.05)。试验表明,血清TM具有一定的特异性,在不同组织肿瘤表达中存在较大的差异,可作为犬肿瘤性疾病的早期诊断依据,且TM联合检测可显著提高恶性肿瘤的检出率。 相似文献
8.
建立慢性砷暴露小鼠动物模型,通过实时定量PCR方法检测慢性砷暴露小鼠乳腺组织中ER、PR mRNA表达量。结果显示,(1)对照组mRNA表达量ER为0.0025±0.0011、PR为0.0190±0.0150;(2)0.05mg/L组ER为0.0085±0.0080、PR为0.0470±0.0180;(3)0.1mg/L组ER为0.0027±0.0003、PR为0.0210±0.0200;(4)0.2mg/L组ER为0.0037±0.0025、PR为0.0390±0.0130;(5)0.4mg/L组ER为0.0029±0.0018、PR为0.0072±0.0031。与对照组比较,各组砷暴露小鼠ER表达均升高,其中0.05mg/L组ER表达差异显著(P0.05);除0.4mg/L组表达降低外,其他各暴露组PR表达也升高,0.05mg/L组PR表达差异极显著(P0.01);0.2mg/L组的PR表达差异显著(P0.05)。结果表明,慢性砷暴露小鼠乳腺组织中ER、PR表达量较对照组发生改变,可能作为一种环境内分泌干扰物发挥雌激素效应进而产生毒性作用。 相似文献
9.
出生后发育时期济宁青山羊乳腺中ERα和PR的分布及其mRNA表达 总被引：1，自引：0，他引：1
本研究旨在探讨济宁青山羊出生后发育过程中乳腺组织内雌激素受体α(ERα)和孕激素受体(PR)免疫阳性细胞的分布及平均光密度和mRNA分子水平的表达规律.应用SP免疫组化法和图像分析方法检测ERα和PR在济宁青山羊乳腺内的分布;以GAPDH为内参基因,qRT-PCR方法检测ERα和PR mRNA的相对表达量.结果表明,ERα和PR阳性染色主要见于乳腺组织内的血管内皮细胞、基质细胞和腺管上皮细胞的细胞核中,偶见胞浆阳性着色,180日龄在腺泡上皮细胞亦可见PR阳性表达;ERα和PR平均光密度自出生～性成熟期呈现逐渐上升的趋势,且性成熟期显著高于出生当天和初情期(P＜0.05),但与180日龄相比无显著差异(P＞0.05).qRT-PCR结果显示,ERα mRNA的表达量自出生～性成熟期呈现逐渐上升趋势,性成熟期最高,极显著高于出生当天和初情期(P＜0.01),180日龄略有下降.PR mRNA表达量自出生当天～性成熟期较低且组间差异不显著(P＞0.05),180日龄的表达量最高且极显著高于其他的3组(P＜0.01).济宁青山羊出生后发育过程中,ERα对腺管伸长和上皮分化以及PR对腺管分支和腺泡发育起促进作用. 相似文献
10.
11.
12.
Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
13.
Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
14.
15.
在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
16.
17.
18.
19.
