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用改良的McCoy’s 5a无血清培养液 (含 3mML -谷氨酰胺、0 1%BSA、2 0ng/ml睾酮、2 5 μg/ml转铁蛋白、10 0ng/ml胰岛素和 4ng/ml硒 ) ,在 96孔培养板中 ,每孔 2 5 0 μl培养液的条件下 ,研究了维生素C和维生素E对腔前卵泡体外发育的影响。结果表明 :在培养液中添加 90 μM的维生素C有利于维持腔前卵泡体外生长时结构的完整性 ,显著提高牛腔前卵泡体外培养 10天的存活率 (P <0 0 5 ) ,但并没有发现对腔前卵泡的生长、发育和成腔有促进作用 (P >0 0 5 ) ;在培养液中添加3 0 μM的维生素E对Φ >13 0 μm的腔前卵泡及其卵母细胞有促进发育和促进卵泡腔形成的趋势 (P >0 0 5 ) ;在培养液中联合添加 12 μM维生素E和 96μM维生素C ,能显著提高牛腔前卵泡体外培养 10天的存活率 (P <0 0 5 ) ,并对牛腔前卵泡的生长和发育有一定的促进作用 ,但差异不显著 (P >0 0 5 )。 相似文献
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一、动物优势卵泡的选择 哺乳动物每个发情周期中有多个卵泡生长发育,腔前卵泡阶段不需要FSH的作用,可进行性地发育到有腔卵泡形成时期,FSH促使其形成腔。排卵前FSH的唯一作用是刺激雌二醇致敏的腔前卵泡~(1-4),同时使上个周期的有腔卵泡迅速发育起来,但并非所有的卵泡都能排卵,这就涉及到哪些卵泡终将成为优势卵泡的问题。 牛在每个发情周期中,有2～3个卵泡发育波,每个波中只有1～2个卵泡变为优势卵泡,其它卵泡则闭锁~(5-9)。目前认为FSH不足以支持所有排卵前卵泡发育,尽管同一时间发育的卵泡受相同血液循环供应,但每个卵泡微环境仍有差异,健康卵泡具有较高雌二醇/雄激素转换比率和较多FSH及LH等受体~(10-,11)。排卵前的 相似文献
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利用McCoy's 5a基础无血清培养系统研究了促卵泡素(FSH)、促黄体素(LH)2种促性腺激素对牛腔前卵泡的体外生长、存活及雌二醇(E2)分泌的影响.结果显示,培养液中单独添加LH对牛腔前卵泡生长影响不大,而单独添加50、100μg/L以上FSH对牛腔前卵泡直径增长和培养后期体外存活有明显的促进作用;当培养液中有10 μg/L LH存在时,2种促性腺激素协同刺激卵泡生长的作用更加明显.添加10 μg/L LH 50μg/L FSH对腔前卵泡体外生长、发育、存活及E2分泌均具有显著的促进作用(P<0.05). 相似文献
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哺乳动物腔前卵泡的研究进展 总被引：1，自引：0，他引：1
哺乳动物的卵巢中,含有数万个卵泡．其中绝大部分为原始卵泡、初级卵泡和次级卵泡等腔前卵泡。然而能发育到成熟并排卵的卵泡为数甚少,约99．9％的卵泡在腔前卵泡阶段闭锁、退化,这无疑是动物遗传和育种资源的极大损失。目前,卵母细咆是休外受精胚胎移植、性别控制、胚胎分割、动物克隆和转基凶等胚胎生物技术研究和开发必不可少的材料,卵母细胞来源匮乏成为制约这些技术研究进展的主要因素,因此卵巢腔前卵泡的开发无疑是解决这一难题的有效途径。本文介绍了腔前卵泡研究的历史和现状以及最新的研究情况．目的是为有关的研究工作提供有益的启示和参考。 相似文献
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《畜牧兽医学报》2020,(6)
旨在研究双调蛋白(AREG)对绵羊小腔卵泡卵母细胞体外成熟(IVM)的影响。本研究从屠宰场绵羊卵巢上采集小腔和中腔卵泡的卵母细胞进行试验,利用自发荧光检测卵母细胞的NAD(P)H和FAD~(++)水平,利用JC-1检测卵母细胞的线粒体膜电位;两种来源的卵母细胞经体外成熟后,比较其卵丘扩展指数(CEI)、第一极体排出率(MII%);比较AREG、AREG+GDF9、AREG+BMP15、AREG+GDF9+BMP15、GDF9+BMP15对小腔卵泡卵母细胞体外成熟后的CEI、MII%及卵母细胞线粒体膜电位的影响;检测了AREG+GDF9+BMP15对小腔卵泡卵母细胞体外成熟后的NAD(P)H和FAD~(++)水平及其受精后发育能力的影响。结果表明,成熟前,小腔卵泡卵母细胞的线粒体膜电位和FAD~(++)水平均显著低于中腔卵泡卵母细胞(P0.05);体外成熟培养后,小腔卵泡卵母细胞的CEI和MII%均显著低于中腔卵泡卵母细胞(P0.05)。与对照组相比,AREG+GDF9+BMP15显著提高了小腔卵泡卵母细胞体外成熟后的CEI、MII%和线粒体膜电位(P0.05),且与中腔卵泡卵母细胞组差异不显著(P0.05);另外,AREG+GDF9+BMP15显著提高了小腔卵泡卵母细胞体外成熟后的NAD(P)H和FAD~(++)水平(P0.05),且与中腔卵泡卵母细胞组差异不显著(P0.05)。与对照组相比,在成熟液中添加AREG+GDF9+BMP15可以明显提高小腔卵泡卵母细胞体外受精后的卵裂率和囊胚率(分别为(43.79±3.69)%、(28.54±4.31)%和(78.99±1.12)%、(47.46±2.50)%,P0.05),而且与中腔卵泡卵母细胞组无显著差异(P0.05)。综上表明,绵羊小腔卵泡卵母细胞的代谢水平及IVM质量较低, AREG在GDF9和BMP15的协同作用下可以显著提高小腔卵泡卵母细胞的代谢水平及IVM质量,并进一步提高小腔卵泡卵母细胞体外受精后的发育能力。 相似文献
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回顾哺乳动物（牛、羊、猪、小鼠、大鼠、兔以及仓鼠等）腔前卵泡的研究历史和现状并探讨腔前卵泡研究在珍稀濒危野生动物（猫科动物、犬科动物和灵长类动物等）上应用的可能性。 相似文献
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研究旨在找出一种水牛腔前卵泡的有效分离方法。采用酶消化结合机械法来分离水牛的腔前卵泡 ,并分别进行培养。来自屠宰场的水牛卵巢 ,先用剪刀去掉髓质部 ,用眼科手术剪刀将其剪碎 ,然后分别用浓度为 0 (CK )、0 0 2 %、0 0 4%、0 0 8%的胶原酶消化 2 0分钟 ,采集腔前卵泡 ,每个卵巢分别获得 3 3 5 6± 6 1 5、40 3 8±6 1 2、47 75± 6 84和 5 2 69± 6 1 5个腔前卵泡 ,试验组的数量明显多于对照组 ( p <0 0 5 )。体外培养 72h后的腔前卵泡存活率分别为 5 0 86%、43 5 5 %、43 0 6%和 2 7 3 1 % ,用 0 0 8%胶原酶消化的存活数显著低于其它组 ( p <0 0 1 )。用胶原酶分离水牛的腔前卵泡 ,适宜的浓度为 0 0 4%。 相似文献
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献13.
Ovine scrapie was first recorded in Cyprus in 1985. Subsequently four dairy goats kept in two mixed flocks with affected sheep developed characteristic clinical signs similar to those seen in sheep. Fifteen goats from the two flocks were examined histologically and neurological lesions consistent with a diagnosis of scrapie were found in the four animals and in three others which had subsequently developed early neurological signs. These lesions were similar to those of naturally-affected sheep although neuronal degeneration and vacuolation were more severe in some cases. 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献15.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献16.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献17.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
相似文献19.
Etsuko Ohta Yuko Nagayama Naoki Koyama Dai Kakiuchi Satoru Hosokawa 《Journal of toxicologic pathology》2016,29(1):31-38
Plasmodium spp. protozoa cause malaria and are known to infect humans and a variety of animal species including macaque monkeys. Here we report both our experience with malaria recrudescence in cynomolgus monkeys (Macaca fascicularis) in a toxicity study and the results of a survey on Plasmodium infection in cynomolgus monkeys imported to Japan for laboratory use. A cynomolgus monkey from the toxicity study presented with severe anemia and Plasmodium protozoa in erythrocytes on a thin blood smear and was subsequently diagnosed with symptomatic malaria. In this animal, congestion and accumulation of hemozoin (malaria pigment) in macrophages were noted in the enlarged and darkly discolored spleen. As a follow-up for the experience, spleen sections from 800 cynomolgus monkeys in toxicity studies conducted between 2003 and 2013 were retrospectively examined for hemozoin deposition as a marker of Plasmodium infection. The origin of the animals included Cambodia, China, Indonesia, and Vietnam. Hemozoin deposition was confirmed in 44% of all examined monkeys. Monkeys from Indonesia showed the highest incidence of hemozoin deposition (approx. 80%). A high prevalence of Plasmodium infection in laboratory monkeys was also confirmed with polymerase chain reaction (PCR) by using Plasmodium genus-specific primers. Although Japan is not a country with endemic malaria, it is important to be aware of the prevalence and potential impact of background infection with Plasmodium spp. and recrudescence of symptomatic malaria in imported laboratory monkeys on pharmaceutical toxicity studies. 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献